Tissue Engineering. Part B, Reviews最新文献

{"title":"Regenerative Potential of Platelet-Rich Plasma-Loaded Scaffolds in Endometrial Damage: A Meta-Analysis.","authors":"Seyedeh-Tarlan Mirzohreh, Zohreh Mohammadi, Yalda Jahanbani, Mahshid Dehghan, Parvin Bastani Alamdari, Armin Imanzadeh Ghazijahani, Soodabeh Davaran, Michael Robert Doran","doi":"10.1177/19373368261448723","DOIUrl":"https://doi.org/10.1177/19373368261448723","url":null,"abstract":"<p><p>This systematic review and meta-analysis evaluated the efficacy of platelet-rich plasma (PRP)-loaded scaffolds for endometrial regeneration in preclinical <i>in vivo</i> models. Endometrial damage, manifesting as endometrial thinning or adhesion formation within the uterine cavity, is a major cause of infertility. While PRP is a promising regenerative therapy, its short half-life limits its effectiveness. The hypothesis was that combining PRP with a hydrogel-based scaffold would provide sustained growth factor release and improve regenerative outcomes compared with monotherapies. The review was conducted per PRISMA guidelines and registered in PROSPERO (CRD420251004274). A comprehensive search of PubMed, Scopus, Web of Science, and Cochrane databases was carried out through April 2025. Seven studies in rodent models met the inclusion criteria. The meta-analysis demonstrated that PRP/scaffold combinations significantly enhanced key outcomes over untreated controls, including endometrial thickness (standardized mean difference [SMD] = 2.85, <i>p</i> < 0.001), gland density (SMD = 4.11, <i>p</i> < 0.001), angiogenesis (SMD = 6.13, <i>p</i> < 0.001), fibrosis reduction (SMD = -5.21, <i>p</i> < 0.001), and embryo implantation rates (SMD = 4.04, <i>p</i> < 0.001). Injectable hydrogels were particularly effective. Compared with PRP alone, the PRP/hydrogels demonstrated improved performance across the majority of structural and functional parameters, though statistical significance varied, partly attributable to variability in scaffold materials and PRP preparation methods. These results suggest that PRP-loaded scaffolds represent a promising approach for improving both structural and functional endometrial regeneration. The therapeutic success is closely tied to the scaffold's degradation kinetics and its ability to preserve PRP bioactivity. This evidence supports the further development of standardized, degradable PRP-scaffold systems for clinical translation in treating endometrial infertility.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261448723"},"PeriodicalIF":4.3,"publicationDate":"2026-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148273189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Round Window Membrane Permeability for Inner Ear Drug Delivery: A Systematic Review.","authors":"Agathe Bedoux, Pauline Ropero, Yohan Bouleau, Raphaël Devillard, Laura Gemini, Damien Bonnard, Olivia Kérourédan","doi":"10.1177/19373368261451024","DOIUrl":"https://doi.org/10.1177/19373368261451024","url":null,"abstract":"<p><p>Sensorineural hearing loss is a major public health concern, yet its treatment remains limited by the anatomical complexity and biological barriers protecting the cochlea. Among these, the round window membrane (RWM) constitutes a key interface for local drug delivery to the inner ear. However, passive diffusion via intratympanic injection is often insufficient, particularly for hydrophilic, large, or negatively charged molecules such as gene therapy vectors. This systematic review aimed to evaluate biomechanical and biochemical strategies to enhance RWM permeability for more efficient and targeted drug delivery to the inner ear, including direct permeability modulation of the RWM properties or indirect enhancement mechanisms increasing drug delivery without altering intrinsic membrane permeability. Following Preferred Reporting Items for Systematic Reviews and Meta-Analysis guidelines, a comprehensive literature search was conducted using the Scopus, MEDLINE/PubMed, Cochrane, and CINAHL electronic databases. <i>In vivo</i> studies and clinical trials involving biochemical or biomechanical strategies to enhance RWM permeability were included. Risk of bias was assessed using the SYRCLE (Systematic Review Center for Laboratory Animal Experimentation) tool. Out of 1776 screened articles, 89 met the inclusion criteria. Four biochemical approaches and three biomechanical strategies were identified, respectively: (1) hydrogels, thermogels, and emulsions, (2) nanosystems, (3) microsystems, and (4) permeabilizers, and (1') sonoporation, (2') acoustic stimulation, and (3') magnetic systems. Most studies reported improved drug delivery to the inner ear or therapeutic efficacy. While earlier research focused on hydrogels, thermogels, emulsions, permeabilizers, and acoustic stimulation for small molecules such as corticoids and antioxidants, recent studies increasingly explore nanosystems, microsystems, sonoporation, and magnetic methods to facilitate the delivery of larger agents, including gene therapy. This review also highlights that while many strategies are already available and effective in animal models, further research is essential to facilitate the clinical translation of both existing and emerging delivery methods.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261451024"},"PeriodicalIF":4.6,"publicationDate":"2026-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148240165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogels as Carriers for Periodontal Ligament Stem Cells in Bone Repair: A Systematic Review.","authors":"Tiago Schlindvein de Araujo, Eduardo Trota Chaves, Thiago Marchi Martins, Wellington Luiz de Oliveira da Rosa","doi":"10.1177/19373368261450070","DOIUrl":"https://doi.org/10.1177/19373368261450070","url":null,"abstract":"<p><p>Hydrogels have emerged as promising delivery vehicles for periodontal ligament stem cells (PDLSCs) due to their biocompatibility and resemblance to the native extracellular matrix. The objective of this systematic review was to evaluate the efficacy of hydrogels as delivery vehicles for PDLSCs in animal models of bone regeneration. This systematic review is reported according to the (Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines and included only <i>in vivo</i> studies evaluating PDLSCs encapsulated in hydrogels for the regeneration of periodontal, alveolar, mandibular, calvarial, or orthodontic defects. Searches were conducted in PubMed, Scopus, Embase, and Web of Science until March 2025. The risk of bias was assessed using the Systematic Review Centre for Laboratory Animal Experimentation tool. Eleven studies were included. Hydrogels included gelatin methacrylate, alginate, chitosan-based matrices, collagen, and thermosensitive poly (lactic-co-glycolic acid)-polyethylene glycol-poly (lactic-co-glycolic acid), functionalized or not with growth factors, bioactive peptides, or nanoparticles. Quantitative microcomputed tomography (micro-CT) analyses assessed bone volume/total volume, bone mineral density, and trabecular thickness. Osteogenic induction typically involved the use of dexamethasone, β-glycerophosphate, and ascorbic acid. Histology confirmed new bone formation and, occasionally, vascularization. In periodontal defects, five studies showed enhanced bone fill and periodontal ligament-like tissue formation. Alveolar bone models reported improved mineralization and neovascularization in two studies. Mandibular defects, evaluated in large animals, exhibited trabecular bone formation. One calvarial and one orthodontic bone loss model reported increased bone density and accelerated deposition, respectively. Due to substantial methodological heterogeneity, a qualitative synthesis was performed. The risk of bias was generally high or unclear in the blinding and allocation domains. The main limitations of this review included heterogeneity across animal models, hydrogel formulations, and outcome measures. Hydrogel-encapsulated PDLSCs promote bone regeneration across diverse defect types, particularly when delivered via injectable and functionalized scaffolds in animal models.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261450070"},"PeriodicalIF":4.6,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148212496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preconditioning Mesenchymal Stem Cells to Enhance Performance in the Degenerate Nucleus Pulposus.","authors":"Auhin Kumar Maparu, Karthikeyan Rajagopal, Keerthana Iyer, Robert L Mauck, Lachlan J Smith","doi":"10.1177/19373368261450742","DOIUrl":"10.1177/19373368261450742","url":null,"abstract":"<p><p>Degeneration of the lumbar intervertebral discs is a leading cause of low back pain, a condition that affects a substantial proportion of the global population. The earliest degenerative changes typically manifest in the central nucleus pulposus (NP) region of the disc and are characterized by elevated local inflammation and a cell-mediated reduction in proteoglycan content and transition to a fibrotic extracellular matrix. The application of adult mesenchymal stem cells (MSCs) to arrest and reverse degenerative changes in the NP has received significant interest due to the availability and superior safety profile of these cells compared with other therapeutic cell types. Preclinically and clinically, however, the efficacy of MSC-based disc treatments is mixed, due in part to the limited ability of these cells to survive and function within the degenerate NP microenvironment. A potential strategy to improve the <i>in vivo</i> survival and anabolic performance of MSCs in the degenerate NP is preconditioning prior to implantation using a variety of biochemical and biophysical stimuli. In this review, we present an overview of existing techniques used for preconditioning MSCs to enhance their therapeutic performance in the degenerate NP. We outline respective advantages and challenges associated with each of these techniques and provide recommendations for their further refinement.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261450742"},"PeriodicalIF":4.3,"publicationDate":"2026-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13339917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148164490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Recent Applications of Stem Cell-Derived Exosomes and Hydrogels in Neurological Disorders.","authors":"Nabil Ajwad, Muzaimi Mustapha, Zamzuri Idris, Si-Yuen Lee","doi":"10.1089/ten.teb.2024.0353","DOIUrl":"10.1089/ten.teb.2024.0353","url":null,"abstract":"<p><p>Neurological disorders such as Alzheimer's disease, Parkinson's disease, and stroke pose significant challenges for conventional therapy due to the complexities of the blood-brain barrier (BBB) and the restricted delivery of drugs to the central nervous system. Exosomes, a type of small extracellular vesicle secreted by nearly all cell types, hold substantial promise as delivery vehicles for therapeutic agents in treating these conditions. Notably, stem cell-secreted exosomes have emerged as particularly effective due to their regenerative potential and natural ability to cross the BBB. Similarly, hydrogels have gained recognition as versatile biomaterials capable of supporting sustained release and targeted delivery of therapeutics. The combination of the regenerative properties of stem cell-derived exosomes (SC-Exos) with the structural and functional benefits of hydrogels offers a promising approach for enhancing neurogenesis, modulating neuroinflammation, and facilitating tissue repair. This review explores the origin, structure, and modifications of exosomes as well as the synthesis and incorporation methods of hydrogels in the therapeutic context for debilitating neurological disorders. It highlights recent advancements in using SC-Exos and hydrogels for therapeutic delivery, addressing both current challenges and future applications. Improving our understanding of hydrogels loaded with SC-Exos for cargo transportation and neural tissue regeneration may pave the way for novel therapeutic strategies.Impact StatementIn this review, we critically appraise the innovative use of stem cell-derived exosomes (SC-Exos) combined with hydrogels for treating neurological disorders, focusing on their dual role in therapeutic cargo delivery and tissue regeneration. We provide a comprehensive overview of the current methods for exosome isolation, stem cell sources, cargo loading techniques, type of hydrogels and their synthesis, exosome-hydrogel incorporation methods, and preclinical applications. This article also offers insights into the current advancements, future trends, and challenges in SC-Exos-loaded hydrogels for neural regeneration and suggests improvements for future research.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"177-190"},"PeriodicalIF":4.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding Intervertebral Disc Cell Populations: Challenges in Isolation and Phenotype Definition.","authors":"Joana R Ferreira, Maria Leonor Moura, Sofia Pilão, Ana Luisa Castro, Morena Fiordalisi, Catarina Leite Pereira, Joana Caldeira, Raquel M Gonçalves","doi":"10.1089/ten.teb.2024.0350","DOIUrl":"10.1089/ten.teb.2024.0350","url":null,"abstract":"<p><p>Degenerative disc disease (DDD) is the main cause of back pain, one of the most impactful musculoskeletal disorders. In the last decades, significant advances have emerged in the field of intervertebral disc (IVD) biology. Despite cumulative knowledge on cell heterogeneity, there is a lack of understanding about the molecular signature of different IVD cell populations and their potential as therapeutic targets. This is not only due to progress in single-cell analysis being recent, but also to difficulties and lack of standardization in the isolation of distinct IVD cell populations based on the phenotypic markers used. Herein, we review the literature on IVD resident cells and the main challenges faced in the source, isolation, and culture of IVD cell subsets that are yet to be overcome. The profiling of IVD subpopulations and their cellular dynamics with development, aging, and disease progression will certainly advance the knowledge on IVD homeostasis and associated diseases, while contributing to the development of game-changing, personalized targeted therapies.Impact StatementDegenerative disc disease (DDD) is a major cause of back pain, with significant societal impact. While advances in intervertebral disc (IVD) biology have revealed cellular heterogeneity, understanding molecular signatures, and therapeutic potential of IVD cell populations remains limited. Addressing challenges in isolating and profiling these cells could transform IVD pathophysiology, enabling the development of personalized, targeted therapies to revolutionize DDD treatment.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"221-237"},"PeriodicalIF":4.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue Engineering for Oral Mucosa Biofabrication: A Systematic Review and Meta-Analysis.","authors":"Miguel A Martin-Piedra, Manuel Albendin-Moreno, Adriana Olivares-Abril, Sara V J Paez-Yepes, Mario Rivera-Izquierdo, David Sánchez-Porras, Ricardo Fernández-Valadés, Antonio España-López, Miguel Alaminos, Ingrid Garzon","doi":"10.1177/19373341251359112","DOIUrl":"10.1177/19373341251359112","url":null,"abstract":"<p><p>Tissue engineering of oral mucosa has emerged as a promising alternative for reconstructing oral lesions. This systematic review and meta-analysis evaluated advancements in the biofabrication of artificial oral mucosa, focusing on its components, methods, and outcomes. A total of 57 studies were included, primarily preclinical <i>in vitro</i> research. The predominant cell sources were primary oral keratinocytes and fibroblasts, with collagen being the most utilized biomaterial. The immersion and air technique was the main biofabrication method. The meta-analysis revealed an average epithelial thickness of 73.18 μm and a maturation score of 5.13/6. <i>In vivo</i> studies indicated a trend toward greater epithelial stratification compared with <i>in vitro</i> studies. The presence of cellularized stroma, decellularized scaffolds, and custom growth factors correlated with increased epithelial thickness although without statistically significant differences. This study provides a comprehensive overview of the current state of tissue-engineered oral mucosa, highlighting its clinical potential.Impact StatementThis systematic review and meta-analysis provides the most comprehensive synthesis to date of biofabrication strategies for tissue-engineered oral mucosa (TEOM). By critically evaluating 57 studies, it evaluates key design variables-such as biomaterials, cell sources, and culture techniques-and their influence on epithelial thickness and maturation. The findings highlight the translational relevance of TEOM for future clinical applications.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"202-220"},"PeriodicalIF":4.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144856424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tendon-Bone Healing: Synergistic Role of Platelets and Mesenchymal Stem Cells in Tissue Engineering.","authors":"Yajie Wu, Xu Wang, Daohong Zhao, Ruke Lin, Xinfu Zhang, Xibei Lin","doi":"10.1089/ten.teb.2024.0333","DOIUrl":"10.1089/ten.teb.2024.0333","url":null,"abstract":"<p><p>Repair of the tendon-bone interface (TBI) remains a significant clinical challenge due to its complex biomechanical environment and hierarchical structure. Conventional surgical approaches often fail to fully reestablish native tissue architecture and function. In recent years, tissue engineering strategies have increasingly emphasized the application of mesenchymal stem cells (MSCs) and platelet-derived products to promote regeneration. MSCs possess multilineage differentiation potential and immunomodulatory capabilities, making them attractive candidates for TBI repair. Platelets, through their rich secretome, orchestrate essential regenerative processes such as cell recruitment, angiogenesis, and immune modulation. This review explores the molecular crosstalk between MSCs and platelets, critically examines current approaches utilizing platelet-rich plasma (PRP)-MSC combinations and platelet-derived exosome therapies and underscores the urgent need for standardization to optimize therapeutic outcomes in PRP-MSC-based regenerative strategies.Impact StatementThis review underscores the critical interplay between platelets and mesenchymal stem cells (MSCs) in tendon-bone healing, highlighting their synergistic roles in promoting osteogenesis, chondrogenesis, angiogenesis, and anti-inflammatory responses. By elucidating the molecular and cellular mechanisms governing these interactions, this work sheds light on potential regenerative therapies that leverage platelet-derived growth factors and MSCs. Such insights aim to optimize healing outcomes for tendon-bone injuries, providing a foundation for innovative clinical strategies that restore the unique and complex structure of the tendon-bone interface.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"191-201"},"PeriodicalIF":4.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multimodal Bioelectronics in Neuromodulation: Liquid Metals, Magnetoelectric, and High-Entropy Oxides.","authors":"Bo Li, Yifan Wang, Kaige Mao, Zheng Tian, Rui Gao, Jiping Dong, Xianzheng Wang, Junyao Cheng, Kai Song, Bing Wu, Xiumei Wang, Zheng Wang","doi":"10.1177/19373368261450074","DOIUrl":"https://doi.org/10.1177/19373368261450074","url":null,"abstract":"<p><p>Multimodal bioelectronic materials have emerged as a promising platform for synergistic neuromodulation, addressing the increasing clinical demand for precise and safe neural interventions. This review highlights recent advances in three pivotal classes of functional materials-liquid metals, magnetoelectric coupling materials, and high-entropy oxides-that offer unique physicochemical properties and versatile fabrication techniques tailored for neural interfaces. We first discuss the clinical significance and advantages of multimodal materials in neuromodulation, followed by an in-depth analysis of the structural characteristics, synthesis methods, and neurointerface applications of these materials. Integrating the latest theoretical models and experimental findings, we elucidate how these materials enable the synergistic application of electrical, magnetic, and mechanical stimuli to enhance neuromodulation efficacy. Despite their promising potential, challenges remain in optimizing biocompatibility, long-term stability, and functional integration. Finally, we provide a forward-looking perspective on the future directions and hurdles for the deployment of multimodal bioelectronic materials in neural disease therapies and intelligent neural interfaces. This review aims to foster a deeper understanding and inspire further innovation in the interdisciplinary field of neuromodulation.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261450074"},"PeriodicalIF":4.6,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148017378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calcium Hydroxylapatite for Facial Rejuvenation: Properties, Mechanisms, and Clinical Applications.","authors":"Yuling Huang, Xinrui Ma, Jiasong Guo","doi":"10.1177/19373368261451012","DOIUrl":"https://doi.org/10.1177/19373368261451012","url":null,"abstract":"<p><p>Calcium hydroxylapatite (CaHA) has evolved from hard tissue applications to become a prospective bioactive filler for facial rejuvenation, offering both immediate volumization and long-term regenerative benefits. This review synthesizes literature up to 2025 to summarize the material properties, mechanisms of action, and clinical applications of CaHA-based fillers, evaluating their safety and efficacy. CaHA-based fillers provide structural support and stimulate collagen synthesis by activating fibroblasts, leading to sustained improvements in skin quality, volume restoration, and contour enhancement. Clinical evidence confirms the effectiveness of both pure CaHA and composite formulations combining CaHA with hyaluronic acid, which integrate rapid correction with prolonged regeneration. However, risks such as nodule formation, technical challenges, and the absence of reversal agents require consideration. CaHA-based fillers represent a significant advancement in minimally invasive facial rejuvenation by merging mechanical support with biological regeneration. Future research should focus on optimizing particle design, standardizing injection protocols, and developing multifunctional carriers to enhance safety and predictability.Impact StatementAs a traditional material for bone and dental repair, calcium hydroxylapatite (CaHA) has surprisingly emerged as a bioactive soft-tissue filler for facial rejuvenation. This review clarifies why this inorganic material can stimulate collagen, restore volume, and improve skin quality, bridging its regenerative mechanisms with clinical practice. By summarizing current applications, efficacy evidence, and safety considerations, we aim to enhance professional understanding, promote wider adoption, and inspire further translational research in minimally invasive aesthetic medicine.</p>","PeriodicalId":23134,"journal":{"name":"Tissue Engineering. Part B, Reviews","volume":" ","pages":"19373368261451012"},"PeriodicalIF":4.6,"publicationDate":"2026-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148006821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}