Scientia Horticulturae最新文献

{"title":"Genome-wide identification and analyses of the AHL gene family in banana (Musa acuminata) and the functional analysis of MaAHL43 in fruit ripening","authors":"Junhui Dong,&nbsp;Linbing Xu,&nbsp;Hongyun Zeng,&nbsp;Xingyu Yang,&nbsp;Yuanli Wu","doi":"10.1016/j.scienta.2024.113948","DOIUrl":"10.1016/j.scienta.2024.113948","url":null,"abstract":"<div><div>Banana (<em>Musa acuminata</em>) is one of the most important and popular fruit crops worldwide. The AT-hook motif nuclear localized (AHL) is a conserved transcription factor family involved in the regulation of plant growth and development and stress response. However, the information of <em>MaAHL</em> genes remain largely elusive. In this study, a total of 48 <em>MaAHL</em> genes were identified based on <em>Masu acuminata</em> genome, which were distributed unevenly across 11 chromosomes. Phylogenetic analysis showed that the MaAHL proteins could be divided into two clades of A and B and subdivided into three types. The <em>cis</em>-regulatory elements in the promoter regions of <em>MaAHL</em> genes were mainly involved in diverse functions including plant growth and development, phytohormones responses, and stress response process. Expression profile analysis showed that <em>MaAHL</em> genes responded to osmotic stress and <em>Fusarium oxysporum</em> infection. In addition, the expression of clade-A <em>MaAHL</em> genes were generally downregulated while clade-B <em>MaAHL</em> genes were up-regulated during fruit ripening in banana. RT-qPCR showed that the expression of <em>MaAHL43</em> gene was significantly up-regulated in middle and late stages of fruit ripening. MaAHL43 is located in nuclear and has no transactivation activity in yeast. Overexpression of <em>MaAHL43</em> gene could promote banana fruit ripening, indicating MaAHL43 might a regulator of fruit ripening in banana. Taken together, this study would provide valuable information for further studies on the functions of <em>MaAHL</em> genes, and providing a new target for further research on the regulation network of banana fruit ripening.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"341 ","pages":"Article 113948"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142988975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Auxin regulates bulbil development by affecting carbohydrate metabolism in Lilium lancifolium","authors":"Mengdi Wang ,&nbsp;Jiamin Li ,&nbsp;Wenqiang Pan ,&nbsp;Qianzi Sang ,&nbsp;Jiahui Liang ,&nbsp;Xuemin Zhang ,&nbsp;Shengnan Kang ,&nbsp;Mingfang Zhang ,&nbsp;Xiuhai Zhang ,&nbsp;Xiaonan Yu ,&nbsp;Yunpeng Du","doi":"10.1016/j.scienta.2025.113972","DOIUrl":"10.1016/j.scienta.2025.113972","url":null,"abstract":"<div><div>Lily bulbils originate from the leaf axils of the middle and upper stems of lilies and play an important role in the reproduction of triploid <em>Lilium lancifolium</em>. The development process of lily bulbils results from cell division and expansion, but the roles of plant hormones and carbohydrate metabolism remains unclear. In this study, we treated <em>L. lancifolium</em> with exogenous indole-3-acetic acid (IAA) and the auxin polar transport inhibitor N-1-naphthylphthalamic acid (NPA) and analyzed morphology, transcriptomics, and gene function during bulbil development. The IAA treatment increased the bulbil diameter and total weight per plant, promoting bulbil development. By constructing an association network of characters and modules, we found that bulbil diameter was significantly negatively correlated with the black module genes and positively correlated with the yellow module genes, which are related to plant hormone and carbohydrate metabolism. We identified key genes in the bulblet system due to bulbil and bulblet development similarities. Silencing the <em>LlSAUR36</em> and <em>LlIAA10</em> genes in the auxin signaling pathway inhibited bulblet development. In the carbohydrate metabolism pathway, we identified two key genes using silencing methods, <em>LlTPS1</em> and <em>LlCSLC5</em>, resulting in phenotypes similar to <em>LlSAUR36</em> and <em>LlIAA10,</em> which inhibited bulbil development. In conclusion, auxin signaling engages in the bulbil development of <em>L. lancifolium</em> by regulating key carbohydrate metabolism genes. This study provides a molecular basis for developing lily bulbils and offers useful clues for the future development of new lily bulbil production methods.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"341 ","pages":"Article 113972"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142989384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PeTB1/2 and PeSLR1 mediate the mechanism of PePIN1b involved in axillary bud dormancy in Phalaenopsis","authors":"Yang Meng ,&nbsp;Guoren He ,&nbsp;Qiyu Zhang ,&nbsp;Mengyi Wang ,&nbsp;Yuxia Lou ,&nbsp;Feng Ming","doi":"10.1016/j.scienta.2025.113949","DOIUrl":"10.1016/j.scienta.2025.113949","url":null,"abstract":"<div><div>The complex architecture of plants, which has a profound impact on their productivity, is shaped by multiple factors, among which branching patterns play an important role. <em>Phalaenopsis</em>, with its exquisite floral beauty and abundant blooms, heavily depends on axillary buds for the development of its blossoms. This study involved the decapitation of <em>Phalaenopsis</em>, followed by a 14-day phase during which axillary buds at various developmental stages—pre-dormancy (S0), dormancy (S1), and dormancy release (S2)—selected for microscopic observation and RNA sequencing. The research revealed <em>PeTB1</em> and <em>PeTB2</em>, members of the TCP class II CYC/TB1 clade, as a key regulator of axillary bud dormancy. The functional validation of these genes, achieved through Virus-Induced Gene Silencing (VIGS), highlighted their essential and overlapping roles in the dormancy process. Intriguingly, during the dormancy release phase, a decrease in auxin levels within the axillary buds was observed, which could be attributed to the auxin efflux transporter PePIN1b. Furthermore, the application of exogenous gibberellic acid (GA) expedited dormancy release, and transcriptome analysis identified <em>PeSLR1</em>, a key DELLA protein gene in the GA signaling pathway, as a key player. The silencing of <em>PeSLR1</em> induced premature dormancy release in the axillary buds, reinforcing its regulatory importance. Notably, PeTB1, PeTB2, and PeSLR1 demonstrated complex interactions both <em>in vivo</em> and <em>in vitro</em>. Particularly, PeTB1 and PeTB2 suppressed the expression of <em>PePIN1b</em>, an auxin transporter gene, and PeSLR1 amplified this inhibitory effect of PeTB1. The mechanism of axillary bud dormancy in <em>Phalaenopsis</em> offers a theoretical basis for enhancing traits in <em>Phalaenopsis</em> production.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"341 ","pages":"Article 113949"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142989517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Localized sensing data-driven efficacy evaluation of heat stress mitigation techniques in ‘Honeycrisp’ apple cultivar","authors":"Basavaraj R. Amogi ,&nbsp;Rakesh Ranjan ,&nbsp;Nisit Pukrongta ,&nbsp;Lav R. Khot ,&nbsp;Bernardita V. Sallato ,&nbsp;Miguel Rene Mogollón ,&nbsp;Carolina A. Torres","doi":"10.1016/j.scienta.2025.113992","DOIUrl":"10.1016/j.scienta.2025.113992","url":null,"abstract":"<div><div>This study was conducted in a commercial ‘Honeycrisp’ apple orchard in 2021 and 2022 season to understand the effectiveness of cyclic evaporative cooling (conventional), continuous fogging, netting and combination of fogging and netting (fognet) in mitigating heat stress on fruits and associated effects on post-harvest fruit quality. Heat stress on fruits was quantified continually during each season using air (T_air) and fruit surface temperature (FST) data collected by a localized crop physiology sensing system. Results indicated that heat stress on fruits in terms of pre- and post-harvest fruit quality was governed by the time for which fruits were subjected to T_air &gt; 32 °C and resultant total heat accumulation. Additionally, effectiveness of heat stress mitigation was influenced by fruits exposure time to direct solar radiation, in season fruit size and crop load. Except netting, all other treatments were effective in removing accumulated heat and maintaining FST below 45 °C fruit sunburn threshold. Although effective, conventional treatment failed to maintain FST below threshold during extreme heat hours (T_air &gt; 32 °C) suggesting adjustments needed in its cyclic operations. FST and T_air data were then contrasted against fruit size, crop load, and percent sunburn at harvest. Similarly, data was contrasted for postharvest performance evaluation, disorders (bitter pit, soft scald) and maturity, until six months of storage. With higher T_air, FST, and smaller fruits, netting delayed fruit maturity in both seasons. Significant difference was observed in bitter pit and soft scald incidence between netting and other treatments only in 2022 season. Insignificant differences in maturity and disorders between fogging, fognet, and conventional implied that fognet has no specific advantage over fogging and conventional cooling technique.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"341 ","pages":"Article 113992"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CsWRKY51, a novel WRKY transcription factor of Camellia sinensis, participates in plant architecture and glutamine accumulation","authors":"Linmu Chen ,&nbsp;Hongwei Dai ,&nbsp;Yiwei Weng ,&nbsp;Kai Zhang ,&nbsp;Shuting Zheng ,&nbsp;Yuanbo Huo ,&nbsp;Lianyu Yuan ,&nbsp;Huarong Tong","doi":"10.1016/j.scienta.2025.113983","DOIUrl":"10.1016/j.scienta.2025.113983","url":null,"abstract":"<div><div>The <em>WRKY</em> genes, belonging to one of the largest families of transcription factors (TFs) in plants, play critical roles in regulating diverse biological processes. In this study, we identified a novel gene from the WRKY IIc subfamily, designated as <em>CsWRKY51</em>, in the tea plant, and confirmed its nuclear localization. Phenotypic analyses of <em>CsWRKY51</em>-overexpressing (OE) plants revealed multiple abnormal traits, including dwarfism, curled leaves, and increased branching and flowering. Additionally, the accumulation levels of amino acids, such as glutamine, asparagine, arginine, and methionine, were significantly elevated in <em>CsWRKY51</em>-OE plants, while chlorophyll content was greatly reduced. Transcriptional profiling indicated that several regulatory pathways, including phytohormone biosynthesis and signaling, photosynthesis, chlorophyll metabolism, carbon metabolism, amino acid metabolism, and nitrogen metabolism were significantly activated in the <em>CsWRKY51</em>-OE plants. Furthermore, the silencing of <em>CsWRKY51</em> in both green and etiolated tea shoots resulted in a significant reduction in glutamine accumulation, accompanied by a substantial decrease in the expression levels of genes encoding glutamine synthetase (GS), glutamate synthase (GOGAT), and glutamate dehydrogenase (GDH). Taken together, these comprehensive analyses demonstrated that <em>CsWRKY51</em> significantly influenced plant aerial architecture, chlorophyll accumulation, and the GS/GOGAT cycle, all of which were implicated in the regulation of glutamine accumulation. This study provides new insights into the functional characterization of <em>CsWRKY</em> genes and the molecular mechanisms underlying plant architecture and glutamine metabolism.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"341 ","pages":"Article 113983"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative strategies for alleviating chromium toxicity in tomato plants using melatonin functionalized zinc oxide nanoparticles","authors":"Shabnam Sharma ,&nbsp;Vaseem Raja ,&nbsp;Sushma ,&nbsp;Aashaq Hussain Bhat ,&nbsp;Naveen Kumar ,&nbsp;Abdulaziz Abdullah Alsahli ,&nbsp;Parvaiz Ahmad","doi":"10.1016/j.scienta.2024.113930","DOIUrl":"10.1016/j.scienta.2024.113930","url":null,"abstract":"<div><div>This study investigates the biosynthesis, characterization, and application of melatonin-functionalized zinc oxide nanoparticles (ZnO NPs@MT) in alleviating chromium (Cr) toxicity in tomato plants. The biosynthesis of ZnO NPs@MT was achieved using leaf extract from <em>Achillea millefolium</em>. The characteristic properties of ZnO NPs@MT were analyzed using UV–Vis spectroscopy, FESEM, XRD, and HR-TEM. UV–Vis analysis revealed a distinct absorption peak at 366 nm, confirming the formation of pure ZnO NPs@MT with a band gap of 3.39 eV SEM and HR-TEM investigations showed uniformly dispersed rod-shaped particles with an average length of 154 nm and a breadth of 24 nm. XRD patterns confirmed the hexagonal crystallinity of the highly pure ZnO NPs@MT synthesized via the green method. Treatment with ZnO NPs@MT effectively mitigated Cr-induced stress in tomato plants. Cr spiked plants treated with ZnO NPs@MT exhibited a significant increase in root and shoot lengths by 119.5% and 134.7%, respectively. Photosynthetic parameters, including chlorophyll content, CO₂ assimilation rate, and stomatal conductance, improved markedly under Cr stress. Antioxidant enzyme activities were enhanced, bolstering the oxidative stress response. Furthermore, ZnO NPs@MT application reduced electrolyte leakage, malondialdehyde, and hydrogen peroxide levels, indicating decreased cellular damage. Increased concentrations of proline and glycine betaine were also observed, contributing to enhanced stress tolerance. Overall, the application of ZnO NPs@MT demonstrates a promising strategy for improving stress resilience in tomato plants, underscoring their potential in sustainable agricultural practices.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"341 ","pages":"Article 113930"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiochemical properties, fruit pathology and shelf life of bananas under pre-harvest bunch spray, post-harvest hand dipping and storage period","authors":"Swosti Debapriya Behera ,&nbsp;Manoranjan Neog ,&nbsp;Stuti Debapriya Behera ,&nbsp;Basudev Behera ,&nbsp;Sanat Kumar Dwibedi ,&nbsp;Alok Nandi ,&nbsp;Nishita Pathak","doi":"10.1016/j.scienta.2025.113977","DOIUrl":"10.1016/j.scienta.2025.113977","url":null,"abstract":"<div><div>Losses during the transport and marketing of banana fruits account for 25-50 %. An experiment was conducted at Jorhat, India in 2017-18 and 2018-19 to study the effects of treatments on the shelf life of bananas. The experiment comprising two bunch spray (pre-harvest) treatments, <em>viz.</em> B₁-Calcium chloride 2 % twice and B₂: Tracel 2 % twice, six solutions for hand dipping (post-harvest), <em>viz.</em> H₁: 1 % chitosan, H₂: 30 ppm benzyl adenine, H₃: 2 % calcium chloride, H₄: 1 % garlic extract, H₅: 150 ppm gibberellic acid and H₆: Control (Distilled water) and four storage periods(days), <em>viz</em>., S₁: 3, S₂: 6, S₃: 9 and S₄: 12 was tried in factorial completely randomized design with three replications. Lower values of physiological loss in fruit weight (PLFW), moisture content (MC), pulp: peel ratio, total soluble solids (TSS), pH and pedicel and peel Pectin methyl esterase (PME) specific activity and higher values of titratable acidity (TTA) and ascorbic acid (AsA) content were recorded under pre-harvest bunch spray of Tracel than CaCl₂ and hand dipping in various solutions than hand dipping in distilled water. At 12^th day of storage, B₂ H₅ and B₁ H₅ were the promising treatments with the minimum PLFW, MC, TSS, and pH and the maximum TTA. The treatment B₂ H₅ recorded the maximum fruit shelf life (15 days) under ambient conditions. Fruit shelf life registered significant and positive correlation with TTA and AsA. The results would help in the development of a suitable strategy to prevent post-harvest losses and boost the economy of banana farmers and traders.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"341 ","pages":"Article 113977"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NLDETR-YOLO: A decision-making method for apple thinning period","authors":"Xiangyu Wang ,&nbsp;Tiebin Yang ,&nbsp;Zhenyu Chen ,&nbsp;Jianjianxian Liu ,&nbsp;Yan He","doi":"10.1016/j.scienta.2025.113991","DOIUrl":"10.1016/j.scienta.2025.113991","url":null,"abstract":"<div><div>Utilizing machine vision to accurately and swiftly remove surplus young fruit during the apple thinning phase is essential for managing crop load and estimating apple production. Fruit thinning operations help balance the nutrition of fruit trees, reduce the risk of tree disease, and prevent the phenomenon of biennial bearing. At this stage, young fruits are characterized by their small size, susceptibility to being obscured, similar colors, and indistinct details, making thinning decision-making difficult. To address these challenges and improve apple thinning decision-making in natural scenes, we developed the NLDETR-YOLO detection model. Firstly, the Efficient Hybrid Encoder from the RT-DETR model is integrated into the feature pyramid (Neck) of YOLOv5n, addressing the optimization difficulties and robustness issues in the YOLO detector that arise from the need for NMS post-processing. Secondly, we introduced the NLBlock attention mechanism, which allows the convolution operation to capture larger dependency relationships. Finally, the loss function to the Slide-loss function was modified to address the imbalance between simple and difficult samples. The experimental results indicate that the proposed method achieved precision (P), recall (R), and mean average precision (mAP) of 80.6 %, 81.2 %, and 86.9 % respectively without overfitting. These represent improvements of 1.9 %, 0.7 %, and 1.4 % compared to the baseline network, with minimal changes to parameters, GFLOPS, and model size. The enhanced NLDETR-YOLO algorithm demonstrates high robustness and real-time detection performance, enabling rapid and accurate thinning decision-making and providing effective support for the automated management of apples during the thinning period in natural environments.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"341 ","pages":"Article 113991"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143348977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light and substrate composition control root exudation rates at the initial stages of soilless lettuce cultivation","authors":"Brechtje R. de Haas ,&nbsp;Eva Oburger ,&nbsp;Marie-Christine Van Labeke ,&nbsp;Emmy Dhooghe ,&nbsp;Danny Geelen","doi":"10.1016/j.scienta.2025.114006","DOIUrl":"10.1016/j.scienta.2025.114006","url":null,"abstract":"<div><div>Plant root exudation is an inherent metabolic process that enhances various functions of the root system like the mobilization of nutrients and interactions with surrounding microbial communities. In soilless crop production, roots are temporally submerged in a nutrient solution affecting the root exudation process. In this study, we asked whether root exudation in soilless cultures is affected by culturing method and substrate composition, important factors determining the root microbial ecosystem. Exploration of different growth conditions revealed that the effect of light quality depended on the substrate used. The impact of light quality and substrate was assessed by growing soilless lettuce in 100 % red light (660 nm), 100 % blue light (450 nm), and white light (full-light spectrum) in deep flow culture, or in 100 % perlite, 100 % potting soil, or mixtures of both growing media. Root exudates were collected at different time points after transplanting. The root exudation rate declined with plant age in all culturing conditions, underscoring its importance during the early stages of development. The total carbon root exudation rate was influenced by light conditions and substrate composition at the earliest timepoint of the culture but not at later growth stages. The total carbohydrate exudation rate was significantly higher under pure blue and red light compared to white light. The impact of light depended on the presence of perlite in the substrate. The total phenolic compound exudation rate was most strongly influenced by the substrate composition and reached the highest level in either pure potting soil or pure perlite. Light and growing media influence the exudation rate at the early stage, suggesting that exudation is an adaptive process of the soilless lettuce culture.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"341 ","pages":"Article 114006"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143348978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of the pigment formation-regulating CYP450 family gives new insights into color improvement in Bougainvillea","authors":"Rong Sun,&nbsp;Shan Liu,&nbsp;Yi Zheng","doi":"10.1016/j.scienta.2025.113997","DOIUrl":"10.1016/j.scienta.2025.113997","url":null,"abstract":"<div><div><em>Bougainvillea</em> is a popular ornamental plant, with modification of flower color is a primary breeding objective. Flower color can be attributed to three classes of pigment: flavonoids, carotenoids, and betalains. The cytochrome P450 monooxygenase gene family plays important roles in pigment biosynthesis. In <em>Bougainvillea</em>, although the CYPs involved in betalains biosynthesis has been reported, the CYPs related to flavonoids and carotenoids biosynthesis have not been characterized. Here, whole-genome analyzes identified 270 <em>BbCYPs</em> in <em>Bougainvillea</em>, which were divided into eight clans via phylogenetic analysis. By exploring the structure, physicochemical properties, chromosome localization and motif composition of BbCYPs, as well as evolutionary relationships with CYPs in other plants, we preliminarily screened 13 potential BbCYPs related to pigment biosynthesis. The expression levels of these genes except <em>BbCYP1</em>, at different developmental stages showed an upward trend. Correlation analysis results indicated that betalains and flavonoids may play major roles in <em>Bougainvillea × buttiana</em> ‘Mrs Butt’ bracts color formation. Among 13 <em>BbCYPs,</em> the <em>BbCYP40</em> and <em>BbCYP220</em> genes exhibited the strongest correlation with betalains and flavonoids, respectively. <em>BbCYP85</em> gene exhibited stronger correlation with carotenoids than <em>BbCYP162</em> gene. These findings provide valuable genetic resources for improving <em>Bougainvillea</em> bract color.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"341 ","pages":"Article 113997"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}