JiaWei Hou , Xu Cai , Yihan Xu , Jungen Kang , Xiaowu Wang , Jian Wu
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引用次数: 0
Abstract
Cabbage (Brassica oleracea var. capitata), a world-widely cultivated leafy vegetable, is characterized by its dense-leaved head formation. Premature bolting, where flowering occurs before head formation significantly reduces cabbage quality and yield. In this study, we employed bulked segregant analysis (BSA) and linkage analysis to identify the quantitative trait loci (QTL) associated with flowering time variation in cabbage. Our analysis revealed a major QTL - designed as qFT2.1, located on chromosome C02. The QTL spans a 12.8 kb region that encompasses five genes. Further analysis identified BoFLC2 (an ortholog of AtFLC) as the candidate gene for qFT2.1. Comparative sequence analysis revealed a 215 bp-insertion in the first intron of BoFLC2 in the early-flowering parental line, along with a 3 bp-insertion and three missense mutations in the second exon. RNA-sequencing revealed that BoFLC2 expression in non-flowering individuals (extremely late flowering) was significantly higher than that in the early-flowering individuals. Analysis of the BoFLC2 sequence across 37 published B. oleracea genomes revealed six haplotypes that are likely associated with flowering time domestication in B. oleracea. Our results suggest that BoFLC2 as a major regulator in flowering time control, has been under selection during the domestication of B. oleracea.
期刊介绍:
Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.