黄角WRKY基因家族的综合分析及XsWRKY30在盐胁迫下的功能特征

IF 4.2 2区 农林科学 Q1 HORTICULTURE
Yu Hui, Weijie Gao, Gaiping Wang, FangFang Fu, Guibin Wang, Fuliang Cao, Xiaoming Yang
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引用次数: 0

摘要

WRKY转录因子作为高等植物中进化保守的调控因子,通过调控转录重编程维持代谢稳态,促进盐胁迫下的适应性反应。然而,黄角(XsWRKY)转录因子(TFs)在盐胁迫下的综合鉴定、表征和功能分析仍然缺乏。黄角是一种多年生木本油料植物,以其卓越的耐盐性而闻名。我们采用全基因组挖掘方法研究了XsWRKY TFs在盐胁迫下的表达谱和生物学功能。该分析确定了43个XsWRKY tf,它们都具有高度保守的结构域。系统发育分析将这些tf分为三个不同的亚群,同一亚群内的基因显示相似的基序组成。共线性分析表明,部分XsWRKY基因起源于片段重复,突出了其结构域的保守性。XsWRKY基因启动子顺式元件分析揭示了与激素信号、非生物应激反应和发育过程相关的调控元件。盐胁迫条件下的转录组学分析突出了不同的表达模式,XsWRKY30成为盐胁迫反应的关键调节因子。实验验证证实XsWRKY30具有转录激活活性,并且定位于细胞核。XsWRKY30在烟草中的瞬时表达可显著降低丙二醛(MDA)的积累,而在拟南芥幼苗和黄角愈伤组织中的稳定过表达可提高抗氧化酶活性和脯氨酸水平,同时MDA含量显著低于野生型对照。此外,过表达xswrky30的拟南芥幼苗和黄角愈伤组织的Na + /K +比值降低,反映了盐胁迫下离子稳态的改善。多个盐胁迫响应基因在转基因品系和愈伤组织中均有上调,表明XsWRKY30在盐胁迫耐受中发挥了正向调控作用。综上所述,这些发现表明XsWRKY30过表达可以缓解渗透胁迫下的氧化损伤和Na⁺的毒性,为XsWRKY TFs在黄角鱼耐盐性中的进化保护和功能意义提供了新的认识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comprehensive analysis of WRKY gene family in yellowhorn (Xanthoceras sorbifolium Bunge) and functional characterisation of XsWRKY30 in salt stress
WRKY transcription factors, as evolutionarily conserved regulators in higher plants, orchestrate transcriptional reprogramming that maintains metabolic homeostasis and facilitates adaptive responses under salinity stress. However, a comprehensive identification, characterisation, and functional analysis of XsWRKY transcription factors (TFs) in response to salt stress remains lacking in yellowhorn (Xanthoceras sorbifolium), a perennial woody oilseed species renowned for its remarkable salt tolerance. We employed a genome-wide mining approach to investigate the expression profiles and biological functions of XsWRKY TFs in response to salt stress. This analysis identified 43 XsWRKY TFs, all possessing a highly conserved domain. Phylogenetic analysis classified these TFs into three distinct subgroups, with genes within the same subgroup displaying similar motif compositions. Collinearity analysis revealed that some XsWRKY genes originated from segmental duplications, highlighting the conservation of their domains. Promoter cis-element analysis of XsWRKY genes revealed regulatory elements associated with hormonal signalling, abiotic stress responses, and developmental processes. Transcriptomic profiling under salt stress conditions highlighted distinct expression patterns, with XsWRKY30 emerging as a key regulator in the salt stress response. Experimental validation confirmed that XsWRKY30 exhibited transcriptional activation activity and was localised to the nucleus. Transient expression of XsWRKY30 in tobacco significantly reduced malondialdehyde (MDA) accumulation, while stable overexpression in Arabidopsis seedlings and yellowhorn calli enhanced antioxidant enzyme activities and proline levels, accompanied by a marked decrease in MDA content compared to wild-type controls. In addition, XsWRKY30-overexpressing Arabidopsis seedlings and yellowhorn calli exhibited a reduced Na⁺/K⁺ ratio, reflecting improved ion homeostasis under salt stress. Several salt stress-responsive genes were also upregulated in the transgenic lines and calli, suggesting that XsWRKY30 played a positive regulatory role in salt tolerance. Overall, these findings demonstrated that XsWRKY30 overexpression alleviated oxidative damage and Na⁺ toxicity under osmotic stress, providing new insights into the evolutionary conservation and functional significance of XsWRKY TFs in yellowhorn salt tolerance.
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来源期刊
Scientia Horticulturae
Scientia Horticulturae 农林科学-园艺
CiteScore
8.60
自引率
4.70%
发文量
796
审稿时长
47 days
期刊介绍: Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.
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