JiaWei Hou , Xu Cai , Yihan Xu , Jungen Kang , Xiaowu Wang , Jian Wu
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引用次数: 0
摘要
白菜(Brassica oleracea var. capitata)是一种世界广泛种植的叶类蔬菜,其特点是其浓密的叶状头状结构。过早抽苔,即在结穗前开花,会显著降低白菜的品质和产量。本研究采用散装分离分析(BSA)和连锁分析(QTL)对白菜开花时间变异相关的数量性状位点进行了鉴定。我们的分析揭示了一个主要的QTL -设计为qFT2.1,位于染色体co2上。QTL横跨12.8 kb的区域,包含5个基因。进一步分析发现BoFLC2 (AtFLC的同源基因)是qFT2.1的候选基因。比较序列分析显示,在早花亲本系BoFLC2的第一个内含子上有一个215 bp的插入,在第二个外显子上有一个3 bp的插入和三个错义突变。rna测序结果显示,BoFLC2在未开花个体(极晚开花个体)中的表达量显著高于早开花个体。对37个已发表的甘蓝基因组中BoFLC2序列的分析揭示了6个可能与甘蓝开花时间驯化相关的单倍型。结果表明,BoFLC2作为花期调控的主要调控因子,在甘蓝驯化过程中得到了选择。
Identification of BoFLC2 as a major gene controlling flowering time in cabbage (Brassica oleracea var. capitata)
Cabbage (Brassica oleracea var. capitata), a world-widely cultivated leafy vegetable, is characterized by its dense-leaved head formation. Premature bolting, where flowering occurs before head formation significantly reduces cabbage quality and yield. In this study, we employed bulked segregant analysis (BSA) and linkage analysis to identify the quantitative trait loci (QTL) associated with flowering time variation in cabbage. Our analysis revealed a major QTL - designed as qFT2.1, located on chromosome C02. The QTL spans a 12.8 kb region that encompasses five genes. Further analysis identified BoFLC2 (an ortholog of AtFLC) as the candidate gene for qFT2.1. Comparative sequence analysis revealed a 215 bp-insertion in the first intron of BoFLC2 in the early-flowering parental line, along with a 3 bp-insertion and three missense mutations in the second exon. RNA-sequencing revealed that BoFLC2 expression in non-flowering individuals (extremely late flowering) was significantly higher than that in the early-flowering individuals. Analysis of the BoFLC2 sequence across 37 published B. oleracea genomes revealed six haplotypes that are likely associated with flowering time domestication in B. oleracea. Our results suggest that BoFLC2 as a major regulator in flowering time control, has been under selection during the domestication of B. oleracea.
期刊介绍:
Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.