综合转录组学和植物激素在发育时间点上的见解来破译花椒的花性别决定

IF 4.2 2区 农林科学 Q1 HORTICULTURE
Yan Chen , Jialing Liang , Zhenshan Liu , Gang Wang , Shanbo Chen , Shijing Feng , Anzhi Wei
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引用次数: 0

摘要

花性别分化是一个重要的生物学过程,对物种进化和生物多样性具有重要意义。花椒(Zanthoxylum armatum)是一种具有重要经济意义的伪雌雄异株植物,具有显著的性可塑性,包括罕见的雌性向雄性的性别转换,但其潜在的调节机制尚不清楚。为了解释这一现象背后的机制,我们在7个关键的花发育阶段进行了高分辨率的时间分析,整合了转录组学和植物激素分析。我们的研究结果表明,两性命运是由动态激素拮抗控制的:雄性花的发育是由生物活性细胞分裂素(如tZR, cZR)和赤霉素(如GA1, GA9)的增加所促进的,而雌性花的命运则与生长素偶联(如IAA-Asp)和细胞分裂素失活有关。同步转录组学分析确定了关键转录因子,包括MADS-box、AP2/ERF、WRKY和GRF家族,这些转录因子在性别之间表达差异,并协调器官的身份和发育。此外,加权基因共表达网络分析(WGCNA)将特定的激素特征与不同的遗传模块联系起来,选择性剪接分析揭示了对减数分裂和花粉功能至关重要的基因的转录后调控。此外,选择性剪接分析揭示了一个复杂的剪接事件网络,有助于调节花发育过程中的基因表达。本研究为植物性别决定提供了一个全面的调控框架,强调了植物激素与转录网络之间复杂的相互作用。我们的发现为植物性别决定的机制提供了基础的见解,并为操纵性表达以提高园艺作物产量的稳定性铺平了道路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Integrated transcriptomic and phytohormonal insights across developmental time points to decipher floral sex determination in Zanthoxylum armatum
Floral sex differentiation is a pivotal biological process with implications for species evolution and biodiversity. Zanthoxylum armatum, a economically important pseudo-dioecious species, exhibits remarkable sexual plasticity, including a rare female-to-male sex transition, yet the underlying regulatory mechanisms remain poorly understood. To decipher the mechanisms underlying this phenomenon, we conducted a high-resolution temporal analysis integrating transcriptomics and phytohormone profiling across seven critical floral developmental stages. Our results revealed that sexual fate is governed by a dynamic hormonal antagonism: male flower development is promoted by a surge of bioactive cytokinins (e.g., tZR, cZR) and gibberellins (e.g., GA1, GA9), while female fate is associated with auxin conjugation (e.g., IAA-Asp) and cytokinin inactivation. Concurrent transcriptomic analyses identified key transcription factors—including MADS-box, AP2/ERF, WRKY, and GRF families—that are differentially expressed between sexes and orchestrate organ identity and development. Furthermore, weighted gene co-expression network analysis (WGCNA) linked specific hormonal signatures to distinct genetic modules, and alternative splicing analysis uncovered post-transcriptional regulation of genes critical for meiosis and pollen function. Additionally, alternative splicing analysis uncovered a complex network of splicing events that contribute to the regulation of gene expression during flower development. This study provides a comprehensive regulatory framework for floral sex determination in Z. armatum, highlighting the intricate interplay between phytohormones and transcriptional networks. Our findings offer foundational insights into the mechanisms of sex determination in plants and pave the way for manipulating sexual expression to enhance yield stability in horticultural crops.
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来源期刊
Scientia Horticulturae
Scientia Horticulturae 农林科学-园艺
CiteScore
8.60
自引率
4.70%
发文量
796
审稿时长
47 days
期刊介绍: Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.
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