Scientia Horticulturae最新文献

{"title":"Genome-wide identification and expression profiling of the NCED gene family in salt and drought stress response of honeysuckle (Lonicera japonica thunb.)","authors":"Yuxing Lei ,&nbsp;Yi Zhang ,&nbsp;Yi Chen ,&nbsp;Huijie Zeng ,&nbsp;Sisi Liu ,&nbsp;Fuyuan Deng ,&nbsp;Gang He ,&nbsp;Dezhi Liao ,&nbsp;Zhongquan Qiao","doi":"10.1016/j.scienta.2025.113980","DOIUrl":"10.1016/j.scienta.2025.113980","url":null,"abstract":"<div><div>9-cis-epoxy carotenoid dioxygenase (<em>NCED</em>) is a pivotal enzyme in the biosynthesis of abscisic acid (ABA), playing an essential role in plant development and responses to abiotic stress. Despite its significance, the <em>NCED</em> gene family in honeysuckle has not been previously characterized<em>.</em> In this study, through whole-genome screening, we identified four <em>NCED</em> genes in honeysuckle, distributed across three chromosomes. Phylogenetic analysis categorized these genes into two distinct groups,indicating evolutionary divergence. Collinearity analysis demonstrated high homology among <em>NCEDs</em> within the <em>Lonicera</em> genus,suggesting conserved roles. Cis-elements analysis revealed the presence of abscisic acid-responsive elements (ABRE) in all four <em>LjNCED</em> promoters, implicating them in ABA-mediated stress responses. Additionally, we investigated the physiological traits of honeysuckle and the expression levels of the <em>NCED</em> gene under different stresses through salt treatment with 400 mM NaCl and drought treatment with 40%±5% moisture content. The results revealed that significant physiological changes occurred in honeysuckle. Expression pattern analysis revealed that <em>LjNCED2</em> and <em>LjNCED4</em> may be significantly involved in the salt stress response, while <em>LjNCED3</em> could be implicated in drought stress response. <em>LjNCED4</em> exhibited a strong correlation with the plant's oxidative stress response in the correlation analysis results. In summary, this study presents the first genome-wide analysis of the <em>LjNCED</em> gene family in honeysuckle, investigating their structural characteristics and potential functions. The valuable insights provided into the <em>LjNCED</em> genes offer new avenues for improving honeysuckle quality and breeding stress-resistant varieties, as well as paving the way for further functional studies of this gene family in plants.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"341 ","pages":"Article 113980"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142989383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical composition of edible flowers: Worldwide research and case study from the Czech Republic","authors":"Lucia Nedorost Ragasová,&nbsp;Jarmila Neugebauerová,&nbsp;Kateřina Patloková,&nbsp;Robert Pokluda","doi":"10.1016/j.scienta.2025.113969","DOIUrl":"10.1016/j.scienta.2025.113969","url":null,"abstract":"<div><div>Edible flowers are nutritionally, culinary and aesthetically valuable addition to food and beverages. Chemical composition of edible flowers (phenols, flavonoids, antioxidant activity etc.) has been analyzed in many studies from various parts of the world. Number of papers focused on content analysis of edible flowers markedly increased from year 2019. Species <em>Calendula officinalis</em> and species of genera <em>Rosa, Dianthus</em>, and <em>Allium</em> are most often studied species of edible flowers worldwide. From the comparison of content analysis from Czech Republic and other parts of the world is not possible clearly assess that climate conditions affect content in expected way. Warmer climate conditions do not always lead to higher phenolic content or antioxidant activity compared to plants from mild climate conditions. This review includes the results from extend study of edible flowers from Czech Republic. Mentioned case study include analysis of total phenolic and flavonoid content, antioxidant activity (DPPH), <span>l</span>-ascorbic acid, nitrates and saponins content of 41 species of edible flowers including both, wild and cultivated species. High values of phenolic content in <em>Origanum</em> sp. were confirmed by Czech and Portugal study. Very high content of <span>l</span>-ascorbic acid in species <em>Primula veris</em> (3838±952 mg/kg FW), <em>Viola odorata</em> (1227±212 mg/kg FW), <em>Armoracia rusticana</em> (1739 ± 245 mg/kg FW), <em>Iris domestica</em> (2068 ± 483 mg/kg FW) and <em>Tropaeolum majus</em> was comparable to results of study from Croatia. However, chemical composition of edible flowers is a very complex and variable issue affected by many factors such climate soil condition, or plant health condition.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"341 ","pages":"Article 113969"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142989386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity and construction of core collection provides new insight for the conservation of edible Allium galanthum in Xinjiang","authors":"Jiaju Wu ,&nbsp;Danhui Liu ,&nbsp;Hafiz Muhammad Wariss ,&nbsp;Hongxiang Zhang ,&nbsp;Mengxu Su ,&nbsp;Wenjun Li ,&nbsp;Zhanjiang Han","doi":"10.1016/j.scienta.2025.113961","DOIUrl":"10.1016/j.scienta.2025.113961","url":null,"abstract":"<div><div><em>Allium galanthum</em> Kar. et Kir. is a significant wild edible plant species that plays an important role in food security and the breeding of onion (<em>Allium cepa</em> L.). In this study, we investigated the genetic diversity, population structure, and core collection construction of 129 <em>A. galanthum</em> individuals from six different populations in Xinjiang, China, using 58,993 high-quality single nucleotide polymorphism (SNP) sites through the genotyping-by-sequencing (GBS) method. The minor allele frequency (MAF), inbreeding coefficient (Fis), observed heterozygosity (Ho), expected heterozygosity (He), and nucleotide diversity (Pi) were calculated as 0.1054, -0.0940, 0.1709, 0.1586, and 0.1594, respectively. Phylogenetic tree and population structure analyses divided <em>A. galanthum</em> individuals into two subgroups: POP1, which included 85 individuals (65.9 % of the total), and POP2, which comprised 44 individuals (34.1 %). The linkage disequilibrium (LD) analysis indicated that the genetic diversity of POP1 was greater than that of POP2. Subsequently, a core collection of 21 samples was constructed, which retained over 98 % of the genetic diversity of the entire collection. Phylogenetic tree and analysis of molecular variance (AMOVA) results showed that the diversity parameters of the core collection were not significantly different from those of the original collection. These findings reveal the genetic diversity and population structure of <em>A. galanthum</em>, which are crucial for the conservation and management of <em>A. galanthum</em> germplasm.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"341 ","pages":"Article 113961"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142989519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pomegranate Aux/IAA9A gene offers new insights into seed development and tissue lignification","authors":"Xin Liu ,&nbsp;Jiyu Li ,&nbsp;Zhen Cao ,&nbsp;Guixiang Li ,&nbsp;Qing Yu ,&nbsp;Mingxia Li ,&nbsp;Yiliu Xu ,&nbsp;Huping Zhang ,&nbsp;Gaihua Qin","doi":"10.1016/j.scienta.2025.113979","DOIUrl":"10.1016/j.scienta.2025.113979","url":null,"abstract":"<div><div>The auxin/indole-3-acetic acid (Aux/IAA) transcription repressor acts as a key switch in the auxin signaling pathway and plays important roles in various aspects of plant development and growth, including fruit and seed development. However, little is known about <em>Aux/IAA</em> genes in pomegranate. We carried out a genome-wide association study to identify candidate <em>Aux/IAA</em> genes involved in pomegranate seed coat development and performed a gene function study of the candidate gene <em>PgIAA9A</em>. Twenty-four <em>PgIAA</em> genes were identified in the pomegranate genome. Among them, <em>PgIAA15, PgIAA16, PgIAA9A, PgIAA3B, PgIAA27B</em>, and <em>PgIAA11</em> exhibited high levels of expression in the seed coat, including the fleshy, edible outer seed coat and the sclerotic inner seed coat. Furthermore, the transcription of these six genes in the inner seed coat was up-regulated by exogenous IAA. Overexpression of <em>PgIAA9Am</em> encoding a stabilized PgIAA9A protein in <em>Arabidopsis</em> resulted in restricted plant growth and development, exemplified by reduced silique length and seed size and decreased lignin contents in stems, leaves, and seeds. Moreover, the expression of genes related to vascular development was reduced. These results shed light on the potential roles of <em>PgIAA9A</em> in pomegranate seed coat development, particularly in terms of seed size and hardness.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"341 ","pages":"Article 113979"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a simple Agrobacterium rhizogenes-mediated hairy root transformation system in sour jujube","authors":"Xiaoya Yang ,&nbsp;Asia Maqbool ,&nbsp;Jiaqi Zang ,&nbsp;Yahong Niu ,&nbsp;Zhiguo Liu ,&nbsp;Lixin Wang ,&nbsp;Mengjun Liu","doi":"10.1016/j.scienta.2025.113984","DOIUrl":"10.1016/j.scienta.2025.113984","url":null,"abstract":"<div><div>Jujube (<em>Ziziphus jujuba</em> Mill.) is an important fruit tree in China. However, due to the high heterozygosity of jujube, the gene transformation system is not maturely established. With the development of <em>A. rhizogenes</em> mediated transformation technology, it was successfully applied to genetic transformation in different woody plant species, which can be employed for gene transformation in jujube. Thus, in order to establish an efficient transformation system for jujube, we obtained hairy roots by infecting sour jujube seedlings with <em>A. rhizogenes</em> which contained <em>35S::GUS-ZjMAPKK4</em> vector under a non-sterile environment, and different treatments on the induction of <em>35S::GUS-ZjMAPKK4</em> hairy roots. The results showed that the hypocotyls of 2-week-old sour jujube seedlings infested with 0 mM AS concentration, which were cultivated at dark condition for 12 h, the transformation efficiency was the highest that the transformed gene hairy roots were successfully induced. Furthermore, to confirm the whether <em>ZjMAPKK4</em> integrated into genome of the hairy roots, the GUS histochemical staining and qRT-PCR analysis were conducted. It showed that the transgenic <em>ZjMAPKK4</em> hairy roots from two growth periods were successfully stained with blue, whereas the wild-type roots from the non-infested seedlings were not stained blue. The relative expression of <em>ZjMAPKK4</em> and <em>GUS</em> gene was significantly higher than that in the wild-type plants, reaching more than 10-folds and 200-folds, respectively. Finally, the transformation efficiency reached 0.66 %. All the results demonstrated that we established a simple <em>A. rhizogenes</em>-mediated hairy root transformation system in sour jujube, which could be used for functional validation analysis of jujube biological genes.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"341 ","pages":"Article 113984"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenomics and evolution of the Acer section Lithocarpa","authors":"Kangjia Liu,&nbsp;Chang Guo,&nbsp;Yuxin Ren,&nbsp;Yuran Bai,&nbsp;Zhixiang Zhang,&nbsp;Wenpan Dong","doi":"10.1016/j.scienta.2025.113988","DOIUrl":"10.1016/j.scienta.2025.113988","url":null,"abstract":"<div><div>Maple trees are known for their graceful appearance and high ornamental value, thanks to their attractive leaf shapes and vibrant autumn foliage. However, the <em>Acer</em> phylogenetic relationships remain unclear, particularly the circumscription of the sections and the relationships between species within those sections. Section <em>Lithocarpa</em> is a typical group with an inconsistent circumscription, containing <em>Lithocarpa</em> and <em>Macrophylla</em> series and about nine species. Here, we used whole chloroplast genomes and nuclear SNP datasets to determine the circumscription of <em>Acer</em> section <em>Lithocarpa</em> infer the phylogenetic relationships, and explore the reticulate evolution in this section. The whole chloroplast genome data suggest that section <em>Lithocarpa</em> is not a monophyletic group, and includes three independent clades, such as series <em>Lithocarpa</em>, series <em>Macrophylla,</em> and the Leipo group including <em>A. yangbiense</em> and <em>A. leipoense</em>. These results support that series <em>Macrophylla</em> should be treated as a distinct section and the taxonomic status of <em>A. yangbiense</em> and <em>A. leipoense</em> in the Leipo group needs to be re-evaluated. We found significant incongruence among the nuclear and chloroplast phylogenies in the series <em>Lithocarpa</em>. The introgression signal detected by the <span>d</span>-statistic in the series <em>Lithocarpa</em> species indicated that introgression was the main cause of cytonuclear discordances. Incomplete lineage sorting (ILS) also contributed to phylogenetic discordance among <em>A. sinopurpurascens, A. tsinglingense</em>, and <em>A. diabolicum</em>. Our results highlight that introgression and ILS are key factors, resulting in the reticulate evolution in <em>Acer</em>.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"341 ","pages":"Article 113988"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tipburn incidence in hydroponically-cultured Chinese cabbage is associated with temporal and spatial coorrdination of ROS regulatory systems","authors":"Takatoshi Kimura ,&nbsp;Kazuma Katano ,&nbsp;Momoka Maeda ,&nbsp;Kazuki Ito ,&nbsp;Arisa Nagao ,&nbsp;Aimi Takanashi ,&nbsp;Nobuhiro Suzuki","doi":"10.1016/j.scienta.2025.113986","DOIUrl":"10.1016/j.scienta.2025.113986","url":null,"abstract":"<div><div>Hydroponic cultivation of crops offers a vital strategy for mitigating yield losses resulting from environmental fluctuations; however, it can lead to necrosis at the leaf apex, known as tipburn. To explore the mechanisms contributing to tipburn incidence, we compared two varieties of Chinese cabbage (<em>Brassica rapa</em> L. ssp. <em>Pekinensis</em>), Haru-torai (HT) and Haru-warai (HW), which have varying sensitivity to tipburn. HT showed higher sensitivity to tipburn compared to HW. Before tipburn incidence, HT exhibited a higher level of O₂^•– and lower activity of SUPEROXIDE DISMUTASE (SOD) compared to HW. A high accumulation of O₂^•– in HT was also observed especially in the tips of leaves during the tipburn incidence. Although H₂O₂ accumulation was higher in HW compared to HT prior to the tipburn incidence, a higher level of H₂O₂ was detected in the tips of leaves in HT compared to HW during the tipburn incidence. These results suggest that tipburn incidence is associated with the temporal and spatial coordination of ROS regulatory systems. This conclusion is further supported by the finding that the sequential application of pyridoxine and ascorbic acid prior to tipburn incidence effectively mitigates this physiological disorder, offering potential to enhance crop yield in controlled facilities.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"341 ","pages":"Article 113986"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Volatile composition, emission pattern, and localization of floral scent emission in crabapple (Malus)","authors":"Qin Peng ,&nbsp;Limeng Zhou ,&nbsp;Junjun Fan ,&nbsp;Jianhua Yue ,&nbsp;Qinqin Xiong ,&nbsp;Dening Duan ,&nbsp;Wangxiang Zhang ,&nbsp;Fangyuan Yu","doi":"10.1016/j.scienta.2025.114000","DOIUrl":"10.1016/j.scienta.2025.114000","url":null,"abstract":"<div><div>Floral scent holds considerable ecological and industrial significance. Frequently cultivated crabapples are scentless and little research has been done on their aromatic properties, which has impeded the ecological and industrial exploration of fragrant crabapple cultivars. M<em>.</em> 'Lollipop', M<em>.</em> 'Hydrangea' and M<em>.</em> 'Prairie Rose' crabapple cultivars, known for their strong floral scent, high resistance and consistent flowering period, were selected to investigate their volatile composition, emission patterns, and floral scent emission localization. Gas chromatography-mass spectrometry analysis identified four primary groups of floral compounds in the three ornamental crabapple cultivars: aliphatic compounds (34 types, 47.88 %), benzenoids/phenylpropanoids (18 types, 25.35 %), terpenes (12 types, 16.90 %), and miscellaneous compounds (7 types, 9.86 %). M<em>.</em> 'Prairie Rose' exhibited the highest diversity of unique compounds, predominantly terpenes. Floral scent emission peaked during the flowering stage (S3) across three crabapples. Dissimilarity coefficient analysis revealed that petals significantly contributed to the overall floral scent in M<em>.</em> 'Lollipop' and M<em>.</em> 'Hydrangea'. Scanning electron microscopy demonstrated that the entire petal functions as a large aromatic gland in three crabapples. These findings provide valuable insights for breeding and industrial applications of fragrant ornamental crabapples.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"341 ","pages":"Article 114000"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143292313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential responses of microstructure, antioxidant defense, and plant hormone signaling regulation in potato (Solanum tuberosum L.) under drought, alkaline salt, and combined stresses","authors":"Ruyan Zhang ,&nbsp;Yong Wang ,&nbsp;Xingxing Wang ,&nbsp;Shujuan Jiao ,&nbsp;Yuan Lu ,&nbsp;Yunyun Du ,&nbsp;Weina Zhang ,&nbsp;Yichen Kang ,&nbsp;Yuhui Liu ,&nbsp;Shuhao Qin","doi":"10.1016/j.scienta.2025.114014","DOIUrl":"10.1016/j.scienta.2025.114014","url":null,"abstract":"&lt;div&gt;&lt;div&gt;In the arid and semi-arid zones of Northwest China, soil drought and alkaline salt stress often occur simultaneously and affect plant growth at multiple levels. Potato (&lt;em&gt;Solanum tuberosum&lt;/em&gt; L.) is a food crop sensitive to drought and alkaline salt stresses and is susceptible to yield loss due to environmental impacts. In recent years, most of the research on abiotic stress response in potato has focused on drought and saline single stresses, and the mechanism of potato response to combined drought-alkaline salt stress and its interactions are still unclear. Therefore, a pot experiment was designed in this study and the potato variety '&lt;em&gt;Atlantic&lt;/em&gt;' was selected as the test material. The effects of drought (25 % PEG-6000), alkaline salt (200 mmol·L&lt;sup&gt;-1&lt;/sup&gt; NaHCO&lt;sub&gt;3&lt;/sub&gt;) and combined drought-alkaline salt (25 % PEG-6000 + 200 mmol·L&lt;sup&gt;-1&lt;/sup&gt; NaHCO&lt;sub&gt;3&lt;/sub&gt;) stresses on growth traits, micro- and ultrastructure, reactive oxygen species, osmoregulatory substances, and antioxidant defenses of potato were investigated using no stress (CK) as a control, leaf photosynthesis and endogenous plant hormones, and also analyzed the changes in the expression patterns of genes related to plant hormone signal transduction under different stresses. The results showed that drought, alkaline salt, and combined stress affected growth, leaf anatomy, and photosynthesis, and increased the accumulation of osmoregulatory substances in potato. The scavenging activities of antioxidant compounds and antioxidant enzymes were enhanced in potato, and combined stress treatments significantly damaged potato more than single stresses. In 2022, combined stress caused a marked increase in H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/sub&gt; (208.7 %) and O&lt;sub&gt;2&lt;/sub&gt;&lt;sup&gt;-&lt;/sup&gt;(455.6 %) content, while in 2023, they increased by 87.5 % and 215.7 %, respectively. SOD, POD, CAT, TPX, APX, GR, GPX and DHAR enzyme activities were increased by 209.13 %, 55.19 %, 152.59 %, 47.13 %, 104.02 %, 347.37 %, 68.45 % and 130.69 % in 2022 compared to CK in the combined stress treatment. In 2023, they increased by 229.81 %, 49.95 %, 160.62 %, 102.16 %, 94.06 %, 505.15 %, 47.00 %, and 121.19 %, respectively. After the stress treatments, the contents of gibberellic acid (GA&lt;sub&gt;3&lt;/sub&gt;) and auxins (IAA) were significantly lower than those in CK, whereas the contents of abscisic acid (ABA), salicylic acid (SA), and brassinosteroids (BRs) increased. Expression of IAA-related genes (AUX1, Aux/IAA, GH3, and SAUR) was up-regulated after stress. ABA-related genes (PYR/PYL, SnRK2, and ABF) were up-regulated after stress, whereas protein phosphatase 2C (PP2C) genes were down-regulated in expression after stress. The GA&lt;sub&gt;3&lt;/sub&gt; receptor GID1 and the F-box protein GID2 were up-regulated after stress. Xyloglucosyl transferase TCH4 gene was up-regulated by stress and positively correlated with changes in BRs content. The TGA transcription factor, PR-1 gene, was induced to up-regulate its expressi","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"341 ","pages":"Article 114014"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of methyl jasmonate-liposomes on 'Kluai Khai' bananas (Musa AA) quality preservation and oxidative response alleviation during room temperature storage","authors":"Li Da ,&nbsp;Pannipa Youryon ,&nbsp;Xiaoyu Suo ,&nbsp;Sirima Sinthusamran ,&nbsp;Janejira Phakawan ,&nbsp;Chairat Techavuthiporn ,&nbsp;Suriyan Supapvanich","doi":"10.1016/j.scienta.2025.113967","DOIUrl":"10.1016/j.scienta.2025.113967","url":null,"abstract":"<div><div>The rapid acceleration of ripening during ambient storage is a primary factor limiting the shelf-life of bananas. Application of methyl jasmonate (MeJA) has been used to enhance the postharvest quality of perishable commodities. This study aimed to investigate the efficacy of MeJA (10⁻³ M) encapsulated in liposomes (MeJA-liposomes) on the postharvest quality of ‘Kluai Khai’ bananas (<em>Musa</em> AA) during storage at room temperature (26 ± 2 °C) for 9 d The MeJA-liposomes exhibited an average particle size of 152.37 ± 1.63 nm with a PDI of 0.21 and a zeta potential value of -33.9 ± 1.02 mV. The MeJA-liposomes treatment exhibited a greater delay in fruit ripening and peel colour development compared to the MeJA (10⁻³ M), blank-liposomes, and control treatments, respectively. MeJA-liposomes reduced the oxidative stress of the peel by decreasing electrolyte leakage, lipoxygenase activity, and the levels of malondialdehyde, α-fanesene, conjugated triene (CT-281) and H₂O₂ in the fruit peel. As the ripening process in untreated bananas progressed, they exhibited greater antioxidant activities, namely in terms of ferric reduction antioxidant capacity and DPPH radical scavenging activity in the peel, compared to the MeJA-liposome-treated bananas. The application of MeJA-liposomes treatment resulted in a postponement in the process of softening and an increase in the levels of total soluble solids content, and BrimA in the banana pulp throughout the storage period. In conclusion, the application of MeJA-liposomes presents a promising method for postponing the ripening process, alleviating the oxidative response in the peel and prolonging the shelf-life of ‘Kluai Khai’ bananas (<em>Musa</em> AA) at room temperature.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"341 ","pages":"Article 113967"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142988963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}