综合转录组学和代谢组学分析揭示“totoga”砧木黄酮介导的冷适应

IF 4.2 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2025-09-01 DOI:10.1016/j.scienta.2025.114414

Zongyi Zhang , Junli Sun , Wenting Zhai , Chunmei Zhu , Zhiyu Liu , Dongliang Zhang , Anqi Xie , Wenchao Shi , Shujun Liu , Jianrong Feng , Baolong Zhao

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引用次数: 0

摘要

为了研究冬青蔬菜生产中的冷害缓解措施，本研究对6个砧木品种‘Totosga’（TTS）、‘景丰808’（JF）、‘torubanm’（TLB）、‘强力’（QL）、‘金麻1号’（JZ）和‘威尔胜根’（WS）进行了低温胁迫下的生理和多组学分析。其中，耐冷基因型Totosga表现出较好的光合完整性、膜稳定性和抗氧化酶活性。比较转录组学和代谢组学分析鉴定出2517个差异表达基因（1015个上调，1502个下调）和2728个差异代谢物（1465个增加，1263个减少）。转录组改变主要影响光合作用相关途径和钙介导的信号转导，而代谢组变化集中在次级代谢过程中。整合组学分析显示，黄酮类生物合成显著富集，鉴定出8个枢纽基因（如PAL2、CHS2、FLS）和16个关键代谢物（如异异黄酮原蛋白、毛蕊异黄酮）作为调控成分。机制研究表明，Totosga通过协调类黄酮代谢重编程来增强冷适应，其特征是(1)黄烷醇生物合成抑制，(2)异黄酮途径激活，以及(3)糖基化/羟基化修饰以稳定代谢物。因此，这些研究结果阐明了砧木冷适应的分子基础，为培育抗逆性强的茄类品种提供了宝贵的遗传资源。

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Integrated transcriptomic and metabolomic analysis reveals flavonoid-mediated cold adaptation in rootstock ‘Totosga’

To investigate chilling injury mitigation in off-season solanaceousvegetable production, this study conducted physiological and multi-omics analyses on six rootstock cultivars—'Totosga' (TTS), 'Jingfeng 808′ (JF), 'Torubam' (TLB), 'Qiangli' (QL), 'Jinzuan No.1′ (JZ), and 'Weiershengen' (WS)—under low-temperature stress. Among these, the cold-tolerant genotype 'Totosga' exhibited superior photosynthetic integrity, membrane stability, and antioxidant enzyme activity. Comparative transcriptomic and metabolomic profiling identified 2517 differentially expressed genes (1015 upregulated; 1502 downregulated) and 2728 differential metabolites (1465 increased; 1263 decreased) under cold stress. Transcriptomic alterations predominantly affected photosynthesis-related pathways and calcium-mediated signal transduction, whereas metabolomic changes were concentrated in secondary metabolic processes. Integrative omics analysis revealed significant enrichment in flavonoid biosynthesis, identifying eight hub genes (e.g., PAL2, CHS2, FLS) and 16 key metabolites (e.g., isoliquiritigenin, calycosin) as regulatory components. Mechanistic investigations demonstrated that 'Totosga' enhances cold adaptation through coordinated flavonoid metabolic reprogramming, characterized by (1) flavanol biosynthesis suppression, (2) isoflavonoid pathway activation, and (3) glycosylation/hydroxylation modifications for metabolite stabilization. Therefore, these findings elucidate the molecular basis of rootstock cold adaptation and provide valuable genetic resources for developing stress-resilient solanaceous cultivars.

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.