Diversification and evolution of the Polycomb group family in nine Rosaceae species and molecular characteristics of PmLHP1

IF 4.2 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2025-09-01 DOI:10.1016/j.scienta.2025.114392

Yuying Lu, Ximeng Lin, Xiao Huang, Feng Gao, Chengdong Ma, Pengyu Zhou, Liping Tong, Fanfan Liu, Dan Cheng, Zhaojun Ni, Zhihong Gao, Ting Shi

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引用次数: 0

Abstract

Polycomb group (PcG) proteins play a crucial role in the regulatory network of plant growth, development, and environmental stress responses, serving as important epigenetic regulators. However, a systematic comparative investigation of PcG proteins across the Rosaceae family is still lacking. In this study, we identified 721 PcG genes in nine Rosaceae species, including 383 members of Polycomb Repressive Complex 1 (PRC1). Phylogenetic analysis, conserved motifs and domain characterization, chromosomal localization, collinearity analysis, and cis-regulatory element prediction indicate that PRC1 proteins participate in plant development and various biological processes. Through extensive phylogenetic analyses, these PcG proteins were classified into nine subfamilies: RING1, BMI1, LHP1, EMF1, VRN1, E(z), Esc, N55, and Su(z)12. These analyses further demonstrated that plant PcG proteins originate from a common ancestor and have shown that PRC1 components are diverged in bryophytes. LHP1 has been shown to be involved in the development of flower epigenetic silencing of genes. Bioinformatics analysis revealed that PmLHP1 exhibits high sequence similarity with orthologs in other woody plants, and its two major structural domains are highly conserved. Given the established role of phase separation in temperature-dependent regulation of plant growth and development, we assessed the likelihood of phase separation among PRC1 components in Rosaceae. Notably, members of the LHP1 subfamily showed high propensity for phase separation. After isolating PmLHP1 from Prunus mume, subcellular localization analysis confirmed its nuclear localization. Furthermore, PmLHP1 exhibited temperature-dependent localization changes under high-temperature conditions. Overall, this study provides new perspectives on the evolutionary dynamics of the PcG family in Rosaceae and the functional role of PmLHP1 in P. mume development.

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蔷薇科9个种Polycomb族的多样性、进化及PmLHP1的分子特征

Polycomb group （PcG）蛋白在植物生长发育和环境胁迫响应的调控网络中发挥着重要作用，是重要的表观遗传调控因子。然而，对整个蔷薇科的PcG蛋白的系统比较研究仍然缺乏。在这项研究中，我们从9个蔷薇科物种中鉴定了721个PcG基因，其中包括383个Polycomb repression Complex 1 （PRC1）成员。系统发育分析、保守基序和结构域表征、染色体定位、共线性分析和顺式调控元件预测表明，PRC1蛋白参与植物发育和各种生物过程。通过广泛的系统发育分析，这些PcG蛋白被划分为九个亚家族：RING1、BMI1、LHP1、EMF1、VRN1、E(z)、Esc、N55和Su(z)12。这些分析进一步表明，植物PcG蛋白起源于一个共同的祖先，并表明PRC1成分在苔藓植物中是分化的。LHP1已被证明参与了花表观遗传沉默基因的发育。生物信息学分析表明，PmLHP1与其他木本植物同源物具有较高的序列相似性，其两个主要结构域高度保守。鉴于相分离在植物生长发育的温度依赖性调节中的作用，我们评估了蔷薇科PRC1组分之间相分离的可能性。值得注意的是，LHP1亚家族的成员表现出高度的相分离倾向。从Prunus mume中分离出PmLHP1，亚细胞定位分析证实其核定位。此外，PmLHP1在高温条件下表现出温度依赖的定位变化。总之，本研究为蔷薇科PcG家族的进化动力学以及PmLHP1在蔷薇发育中的功能作用提供了新的视角。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.