Scientia HorticulturaePub Date : 2026-10-01Epub Date: 2026-10-24DOI: 10.1016/j.scienta.2026.115261
S. Gutiérrez-Gordillo, I.F. García-Tejero, V.H. Durán-Zuazo, V. Hernandez-Santana, María M. Borrego-Marín
{"title":"Physiology-driven precision irrigation for almond nut quality and profitability under contrasting Mediterranean environments","authors":"S. Gutiérrez-Gordillo, I.F. García-Tejero, V.H. Durán-Zuazo, V. Hernandez-Santana, María M. Borrego-Marín","doi":"10.1016/j.scienta.2026.115261","DOIUrl":"10.1016/j.scienta.2026.115261","url":null,"abstract":"<div><div>This article evaluates the physiological, quality, and economic performance of three commercial almond cultivars (Guara, Marta, and Lauranne) subjected to regulated and sustained deficit irrigation strategies in two orchards with contrasting agroeconomic conditions. Moving beyond conventional agronomic assessments, the study integrates physiological indicators commonly used in precision irrigation scheduling, including leaf water potential and stomatal conductance, to interpret cultivar-specific responses and assess these strategies within a data-informed irrigation management framework. Results indicate that deficit irrigation can enhance resource-use efficiency by achieving water savings greater than the proportional reduction in yield, without compromising kernel quality and even improving attributes linked to sensory quality and potential market value. These gains in quality and efficiency improve irrigation water productivity and profitability, providing a mechanism to ensure economic performance at the orchard level and supporting deficit irrigation as a viable precision-irrigation strategy under water scarcity conditions.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"368 ","pages":"Article 115261"},"PeriodicalIF":4.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149254396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Methyl jasmonate induces glucosinolate accumulation by transcriptional activation of biosynthetic genes in kale (Brassica oleracea var. acephala DC.) seedlings","authors":"Shuai Yan, Yafang Luo, Meihui Li, Zezheng Song, Tiantian Zhou, Guohua Dong, Huan Wang, Yushu Wang","doi":"10.1016/j.scienta.2026.115206","DOIUrl":"10.1016/j.scienta.2026.115206","url":null,"abstract":"<div><div>Methyl jasmonate (MeJA) serves as a pivotal signaling molecule orchestrating plant secondary metabolism. However, the regulatory influence of MeJA on glucosinolate (GSL) profiles, associated gene expression, and genotype-dependent responses in kale (<em>Brassica oleracea</em> var. <em>acephala</em> DC.) seedlings remains to be further investigated. Here, we investigated the responses of two kale lines ('W3′ and 'R6′) to foliar application of 100 μmol/L MeJA. Using ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF–MS/MS), we identified 15 GSL components distributed across the two lines. Notably, MeJA treatment preferentially promoted the accumulation of GSLs with documented or proposed health benefits, among which 1-hydroxyindol-3-ylmethyl GSL (1HGBS) exhibited the most pronounced increase, with 3.90-fold and 1.13-fold increases in 'W3′ and 'R6′, respectively, relative to the control level. Quantitative real-time PCR (qRT-PCR) analysis revealed that MeJA significantly upregulated the expression of key biosynthetic genes involved in GSL production. Specifically, <em>BoMYB51</em> expression was upregulated 11.00-fold in 'W3′ and 8.48-fold in 'R6′ relative to the control. In addition, MeJA enhanced antioxidant capacity, as evidenced by increased total phenolics and flavonoid contents, as well as higher DPPH radical scavenging activity in both lines. Collectively, these findings demonstrate that exogenous MeJA induces GSL accumulation in kale seedlings through transcriptional activation of biosynthetic genes, accompanied by genotype-specific enhancement of antioxidant capacity. Our results provide insights into the genotype-dependent improvement of kale's functional quality through MeJA application.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"368 ","pages":"Article 115206"},"PeriodicalIF":4.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149088016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ArMPK4-ArPAT1 interaction mediating disease resistance in the medicinal orchid Anoectochilus roxburghii","authors":"Weiqiang Kuang, Shuyan Zhou, Sirui Xu, Chuyu Lin, Lanying Pan, Qingsong Shao, Ying Zheng","doi":"10.1016/j.scienta.2026.115226","DOIUrl":"10.1016/j.scienta.2026.115226","url":null,"abstract":"<div><div>Stem rot disease poses a major threat to the cultivation and yield of the valuable medicinal orchid <em>Anoectochilus roxburghii</em>. Although <em>MPK4</em> acts as a key regulator of plant disease resistance, its precise regulatory mechanism is not fully understood. In this study, we systematically identified 28 putative <em>ArMPK</em> and 19 <em>ArPAT1</em> genes based on transcriptomic data. Combined expression profiling and co-expression networks analysis highlighted a strong correlation between the initially screened candidate transcripts <em>ArMPK4-6</em> and <em>ArPAT1-4</em>, which were subsequently curated and designated as <em>ArMPK4</em> and <em>ArPAT1</em> for functional validation. Their tightly correlated expression patterns were further confirmed by RT-qPCR. Split‑luciferase complementation assays verified the physical interaction between ArMPK4 and ArPAT1 at the plasma membrane. Furthermore, phosphorylation site prediction identified several potential regulatory residues within ArMPK4, including Ser-148, Ser-263, and Tyr-28. Structural analysis suggested that Ser-148 and Ser-263 reside close to key functional regions, indicating their potential role in regulating protein activity. Collectively, these findings elucidate a previously unreported ArMPK4-ArPAT1 interaction module and provide a scientific foundation for improving disease resistance in <em>A. roxburghii</em>.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"368 ","pages":"Article 115226"},"PeriodicalIF":4.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149150758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Scientia HorticulturaePub Date : 2026-10-01Epub Date: 2026-10-08DOI: 10.1016/j.scienta.2026.115222
Jana Dutordoir, Benny De Cauwer, Mirte Boonaert, Bart Vanhoutte, Serge Remy, Kathy Steppe
{"title":"Mechanistic modelling of daily hydraulic resistance and capacitance in pear to support irrigation decisions","authors":"Jana Dutordoir, Benny De Cauwer, Mirte Boonaert, Bart Vanhoutte, Serge Remy, Kathy Steppe","doi":"10.1016/j.scienta.2026.115222","DOIUrl":"10.1016/j.scienta.2026.115222","url":null,"abstract":"<div><div>Translating continuous plant sensor signals into actionable irrigation rules remains a practical bottleneck in fruit tree orchards. Integrating sensor outputs with a compact water flow and storage model offers a clear pathway toward simpler, physiology-based control. We assessed this model in a rainfed ‘Conference’ pear orchard to test its ability to reproduce diurnal stem water potential (Ψ<sub>stem</sub>) and yield daily stem hydraulic resistance (<em>R</em><sub>stem</sub>) and capacitance (<em>C</em><sub>stem</sub>) values. Two mature trees were monitored in 2024 (DOY 238–266). Using sap flow rate and soil water potential measurements as model inputs, simulated Ψ<sub>stem</sub> closely matched microtensiometer data (R² = 0.95), indicating excellent model reliability. Model-calibrated <em>R</em><sub>stem</sub> was invariant across the observed Ψ<sub>stem</sub> range and stable over time, with mean ± SD values of 0.308 ± 0.037 and 0.683 ± 0.052 MPa h kg⁻¹ for Tree 1 and 2, respectively. The temporal dynamics of both <em>R</em><sub>stem</sub> and <em>C</em><sub>stem</sub> were similar across trees, with <em>C</em><sub>stem</sub> declining as Ψ<sub>stem</sub> became more negative. Model-calibrated <em>R</em><sub>stem</sub> corresponded well with the van den Honert concept under steady-state conditions but diverged on more dynamic days, with daily standard deviations being markedly smaller for the model, indicating greater robustness to transient conditions. This study provides proof of concept that daily <em>R</em><sub>stem</sub> dynamics can support future irrigation decision-making, while emphasizing the need for tree-specific baselines across phenological stages and interpretation in conjunction with Ψ<sub>stem</sub>.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"368 ","pages":"Article 115222"},"PeriodicalIF":4.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149150767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Scientia HorticulturaePub Date : 2026-10-01Epub Date: 2026-09-29DOI: 10.1016/j.scienta.2026.115195
Fatima Zahra Akhrif, Pedro Valverde, Antonio Pascual, Carlos Trapero
{"title":"Phenological growth stages of pistachio (Pistacia vera L.) according to the extended BBCH scale: Description and chronological characterization in a Mediterranean environment","authors":"Fatima Zahra Akhrif, Pedro Valverde, Antonio Pascual, Carlos Trapero","doi":"10.1016/j.scienta.2026.115195","DOIUrl":"10.1016/j.scienta.2026.115195","url":null,"abstract":"<div><div>Pistachio (<em>Pistacia vera</em> L.) is considered one of the most important tree nuts worldwide, for its high nutritional and economic value. Recently, its cultivation is expanding into different regions which climatic conditions are highly affected by global warming, particularly Mediterranean regions. In this context, understanding the phenological response of this plant is becoming an essential tool for optimizing yields and quality. In other species, this monitoring is possible thanks to the BBCH scale. This study represents a detailed characterization of the phenological growth stages of pistachio, including five female cultivars: ‘Kerman’, ‘Sirora’, ‘Aegina’, ‘Larnaka’ and ‘Lost Hills’ and two male cultivars represented by ‘Peters’ and ‘Randy’, using this scale in order to provide an efficient tool for researchers and growers. Through monitoring of two consecutive years (2024 and 2025), we codified and described both vegetative and reproductive phenology including 56 stages in total. For a clear identification of these stages, our study provides illustrative images for each phase. In addition, our data has helped to determine the chronological progression of growth in some of the most commonly grown cultivars under the climatic conditions of southern Spain. These findings are expected to enhance the efficiency of pistachio research, management and breeding.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"368 ","pages":"Article 115195"},"PeriodicalIF":4.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149085384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Scientia HorticulturaePub Date : 2026-10-01Epub Date: 2026-10-13DOI: 10.1016/j.scienta.2026.115236
Xianqi Fei, Yunfei Zhuang, Shumei Zhao
{"title":"Diffuse light in protected horticulture: Crop responses, covering materials, and applications across greenhouse types and climates","authors":"Xianqi Fei, Yunfei Zhuang, Shumei Zhao","doi":"10.1016/j.scienta.2026.115236","DOIUrl":"10.1016/j.scienta.2026.115236","url":null,"abstract":"<div><div>Greenhouse light management increasingly focuses not only on radiation transmission, but also on the spatial and temporal distribution of photosynthetically active radiation within horticultural crop canopies. Diffuse-light covering materials are increasingly used in greenhouse horticulture to redistribute incoming solar radiation within crop canopies, but their benefits for horticultural crops remain inconsistent because light transmission, diffuseness, scattering pattern, canopy architecture, greenhouse type and regional radiation climate interact. This review synthesises current evidence on diffuse light in protected horticulture, with emphasis on its effects on canopy light environment, photosynthesis, yield formation and quality of greenhouse horticultural crops. Diffuse light can improve crop performance through three coupled mechanisms: deeper vertical penetration and greater horizontal uniformity of photosynthetically active radiation; reduced short-term shadefleck fluctuations, upper-canopy light and heat stress; and enhanced canopy-scale photosynthesis and radiation-use efficiency when redistributed photons are intercepted by photosynthetically active leaves. Reported benefits are most consistent in high-wire horticultural crops, especially tomato and cucumber, where diffuse light can redistribute radiation to middle and lower leaves, with yield increases commonly around 5.5–15.0% and 4.3–20.5%, respectively. By contrast, the response is less stable in compact or shallow-canopy crops. Existing studies also show that haze alone cannot reliably predict horticultural crop response, because scattering angle, total transmission and canopy structure jointly determine the actual light environment experienced by plants. Future applications should therefore move beyond material selection based only on diffuseness toward crop-specific and climate-specific matching of covering materials, greenhouse types, canopy architecture and regional radiation conditions.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"368 ","pages":"Article 115236"},"PeriodicalIF":4.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149202219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Scientia HorticulturaePub Date : 2026-10-01Epub Date: 2026-09-27DOI: 10.1016/j.scienta.2026.115184
Gladys Karungari, Ginat Raphael, Lior Avraham, Sharon Alkalai-Tuvia, Elazar Fallik, Harry S. Paris, Carmit Ziv
{"title":"Auxin-induced parthenocarpy as an alternative to pollination of trellised sweet acorn squash grown under protection in fall and early winter","authors":"Gladys Karungari, Ginat Raphael, Lior Avraham, Sharon Alkalai-Tuvia, Elazar Fallik, Harry S. Paris, Carmit Ziv","doi":"10.1016/j.scienta.2026.115184","DOIUrl":"10.1016/j.scienta.2026.115184","url":null,"abstract":"<div><div>Sweet-fruited acorn squash are a premium-priced vegetable in Israel and are produced under field conditions in the spring and summer. They are also produced from plants grown trellised in screenhouses in the fall, for marketing after storage during the winter months. Ordinarily, in these screenhouses, bumblebees are introduced for pollination when the plants begin to flower. However, viability of pollen and activity of bees are subject to the vagaries of environmental factors. Our objective was to determine if spraying the plants with auxin during flowering to induce parthenocarpic fruit set could act as a viable alternative to the bees. Sweet acorn squash seedlings were transplanted into a screenhouse in the fall and subjected to three treatments, spraying with auxin to induce parthenocarpic fruit set, pollination by bumblebees, or spraying with auxin plus bee pollination. The treatments were then compared for yield and fruit quality, including physical traits, carbohydrate and antioxidant contents, and several sensory parameters, at harvest and after one, two, or three months of storage at 15 °C, 95% RH. The fruits produced by the auxin-sprayed plants were heavier and had a larger circumference, with noticeably thicker mesocarp and undeveloped seeds. Yields of auxin-treated plants were comparable to those subjected to bee pollination. Total soluble solids (TSS), sugar, and antioxidant contents of auxin-treated and bee-pollinated derived fruits were similar. However, fruits developing from the auxin treatment contained more starch at harvest and retained more starch during storage, suggesting that auxin delayed fruit ripening and aging.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"368 ","pages":"Article 115184"},"PeriodicalIF":4.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149049170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Scientia HorticulturaePub Date : 2026-10-01Epub Date: 2026-09-27DOI: 10.1016/j.scienta.2026.115192
Catalina Pinto, Emilio Vilches, Gabino Reginato
{"title":"A GAMLSS-based approach for modeling fruit weight distributions in ‘Hayward’ kiwifruit orchards","authors":"Catalina Pinto, Emilio Vilches, Gabino Reginato","doi":"10.1016/j.scienta.2026.115192","DOIUrl":"10.1016/j.scienta.2026.115192","url":null,"abstract":"<div><div>Fruit size distribution is a key determinant of commercial value in kiwifruit production and an important target for orchard management and harvest planning. This study aimed to model the full distribution of fruit weight over time in ‘Hayward’ kiwifruit under Mediterranean conditions in central Chile using a non-destructive and distributional modeling approach. Fruit growth was monitored over three consecutive seasons in commercial orchards using repeated measurements of fruit length and maximum and minimum equatorial diameters. An empirical model derived from destructive measurements accurately estimated fresh fruit weight from linear dimensions, enabling non-destructive assessment throughout fruit development. Estimated fruit weight data were subsequently modeled as a function of days after full bloom (DAFB) using the Generalized Additive Models for Location, Scale, and Shape (GAMLSS) framework. Among the candidate distributions evaluated, the Box-Cox Cole-Green original (BCCGo) distribution provided adequate performance across orchards and seasons. The selected GAMLSS modeled temporal changes in fruit weight location and dispersion while accounting for distributional asymmetry, including the progressive increase in variability observed toward harvest. In contrast to traditional mean-based growth models, the proposed framework described fruit growth as a dynamic distributional process. External validation over three independent seasons showed a systematic over-prediction of 3–6% at 140-152 DAFB that increased with the extrapolation horizon, a mean coverage of 77% for the predicted 80% intervals, and a consistent advantage of the distributional predictions over classical point forecasts under the continuous ranked probability score. Overall, this study demonstrates the potential of distributional regression models for fruit size forecasting and decision support in commercial kiwifruit production systems.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"368 ","pages":"Article 115192"},"PeriodicalIF":4.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149049175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Scientia HorticulturaePub Date : 2026-10-01Epub Date: 2026-10-12DOI: 10.1016/j.scienta.2026.115219
Giuseppe Montanaro, Maria Calabritto, Alba Nicoletta Mininni, Bartolomeo Dichio
{"title":"Soil moisture and vapour pressure deficit predict trunk water potential in field-grown kiwifruit","authors":"Giuseppe Montanaro, Maria Calabritto, Alba Nicoletta Mininni, Bartolomeo Dichio","doi":"10.1016/j.scienta.2026.115219","DOIUrl":"10.1016/j.scienta.2026.115219","url":null,"abstract":"<div><div>A dynamic model has been developed to predict trunk water potential (Ψ<sub>trunk</sub>) from soil water content (<em>SWC</em>) and air vapour pressure deficit (<em>VPD</em>) in field-grown kiwifruit. The model included a capacitor (trunk tissue) apart of soil in order to account for the time lag between transpiration and Ψ<sub>trunk</sub>. Sub-models were defined from known, calibrated or specifically developed relationships linking <em>SWC</em> or <em>VPD</em> to hydraulic resistances of the whole soil-leaf path and of the storage compartment, plant leaf area and transpiration. Estimation of soil water potential from <em>SWC</em> was also included. The estimations of Ψ<sub>trunk</sub> (Ψ<sub>model</sub>) were compared with <em>in vivo</em> measurements via microtensiometers installed in vines under full irrigation or progressively droughted. The resulting Ψ<sub>model</sub> values were closely related to actual ones showing a median <em>R</em><sup>2</sup> up to approx. 0.95. The model revealed that the daily water released by the compartment to the transpiration stream (up to 0.22 m³ ha⁻¹) makes the orchard almost entirely dependent on root water uptake, posing a significant challenge for irrigation management. The main advantage of the model proposed is the limited number (two) of operational inputs variables supporting the employment of a combination of model and sensors to enable easy, and relatively low-cost monitoring of plant water status and in turn irrigation management.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"368 ","pages":"Article 115219"},"PeriodicalIF":4.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149202192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Foliar-applied hempseed press cake-derived protein hydrolysate mitigates salinity stress in hydroponic lettuce with improved ion balance, enhanced antioxidant responses, and metabolomic changes","authors":"Bhornchai Harakotr, Napat Srisuwannaphat, Lalita Charoensup, Yaowapha Jirakiattikul","doi":"10.1016/j.scienta.2026.115240","DOIUrl":"10.1016/j.scienta.2026.115240","url":null,"abstract":"<div><div>Salinity limits lettuce production by reducing growth, disturbing ion balance, and promoting oxidative stress. This study evaluated the effects of foliar-applied hempseed press cake-derived protein hydrolysate (HPH) on salinity-stressed hydroponically grown lettuce, with emphasis on ion balance, antioxidant responses, and leaf metabolomic changes. The experiment consisted of five treatments: control (nutrient solution with distilled water foliar spray), NaCl (40 mM NaCl nutrient solution with distilled water foliar spray), NaCl + HPH0 (40 mM NaCl nutrient solution with carrier formulation foliar spray), and NaCl + HPH7.5 and NaCl + HPH10 (40 mM NaCl nutrient solution with 7.5 and 10.0% (<em>w</em>/<em>v</em>) HPH foliar spray, respectively). Growth, biomass, mineral contents, photosynthetic pigments, antioxidant responses, and metabolomic profiles were assessed. Salinity markedly reduced shoot and root biomass, with shoot fresh weight declining by 52% relative to the control. HPH mitigated biomass loss under salinity, increasing shoot fresh weight by up to 60% relative to NaCl alone. Root biomass under both HPH treatments was not significantly different from the control. HPH also reduced Na⁺ accumulation, increased K⁺ concentration, lowered the Na⁺/K⁺ ratio, and increased Ca²⁺ concentration relative to NaCl treatment. HPH-treated plants showed higher SOD, CAT, and APX activities and greater phenolic and flavonoid accumulation, especially at HPH10. Metabolomic analysis revealed treatment-associated differences in metabolites mapped to amino acid-, nitrogen-, and stress-related pathway categories under HPH treatment. In conclusion, HPH may mitigate salinity-induced growth inhibition in lettuce by supporting biomass retention, ion balance, antioxidant responses, and metabolic adjustment under saline conditions.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"368 ","pages":"Article 115240"},"PeriodicalIF":4.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149210501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}