Scientia Horticulturae最新文献

{"title":"Effects of exogenous methyl jasmonate on the growth and physiological characteristics of medicinal Chrysanthemum morifolium Ramat under drought stress","authors":"Yanqiu Qiu ,&nbsp;Lixin Zhang ,&nbsp;Dandan Yang ,&nbsp;Jiaying Chen ,&nbsp;Xiaoshen Zhang ,&nbsp;Hongrui Zhang","doi":"10.1016/j.scienta.2025.114278","DOIUrl":"10.1016/j.scienta.2025.114278","url":null,"abstract":"<div><div>Drought severely impacts plant growth, inducing oxidative stress. Although methyl jasmonate (MeJA) mitigates drought effects in many plants, its physiological mechanism in medicinal <em>Chrysanthemum morifolium</em> remains unclear. We used different concentrations (0.1, 1, 10, 100, 1000 mg <span>l</span>^-1) of methyl jasmonate (MeJA) on foliage to explore whether exogenous MeJA can improve the drought resistance of medicinal <em>C. morifolium</em> by affecting the growth and physiological characteristics, and to determine a suitable exogenous MeJA concentration. Results showed that with the prolongation of drought stress, plant height, crown width, leaf area and leaf thickness of the two species of medicinal <em>C. morifolium</em> in the seedling stage first increased and then decreased, the green leaf rate gradually decreased, the above-ground and below-ground dry weights increased, the relative water content (RWC) of leaves decreased, the relative chlorophyll content (SPAD) firstly increased and then decreased, the content of MDA increased, and the activities of antioxidant enzymes (SOD, POD and CAT) all increased before being followed by a decrease. Our comprehensive evaluation analysis by the affiliation function concluded that it was more effective to treat ‘Huaibaiju’ with 10 mg <span>l</span>^-1 MeJA and ‘Wuyuanhuangju’ with 1 mg <span>l</span>^-1 MeJA, which could effectively alleviate the damage caused by drought stress. The study provides insights into the potential application of MeJA in enhancing the resilience of medicinal plants under drought stress, which could contribute to the sustainable cultivation of medicinal <em>C. morifolium</em> in arid regions.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"354 ","pages":"Article 114278"},"PeriodicalIF":3.9,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aroma potential assessment in recovered minority red grape varieties by characterization with UHPLC-TOF/MS of intact glycosidic precursors","authors":"Cristina Cebrián-Tarancón ,&nbsp;Mirko De Rosso ,&nbsp;Annarita Panighel ,&nbsp;Riccardo Flamini ,&nbsp;Gonzalo L. Alonso ,&nbsp;M. Rosario Salinas ,&nbsp;Juan L. Chacón-Vozmediano ,&nbsp;Rosario Sánchez-Gómez","doi":"10.1016/j.scienta.2025.114283","DOIUrl":"10.1016/j.scienta.2025.114283","url":null,"abstract":"<div><div>In the context of climate change, there is a growing interest in investigating novel grapevine varieties that may bolster the resilience of current viticultural practices. The analysis of intact glycoside aroma precursors is crucial for understanding the enological potential of grape varieties, as these compounds significantly influence the sensory attributes of wines. This study characterizes the profile of intact glycoside aroma precursors in two recovered minority red grape varieties, Moravia Agria and Tinto Fragoso, cultivated under varying water deficit conditions: deficit (moderate water stress conditions) and survival (severe water stress) irrigation. The analysis was conducted using UHPLC-TOF Mass Spectrometry and compared with Tempranillo grapes as reference. In total, 20 glycosidic compounds were identified in the minor grape varieties, spanning chemical classes such as benzenoids, aliphatic alcohols, monoterpenes, and norisoprenoids. Tempranillo exhibited a higher concentration of glycoside aliphatic alcohols, whereas grapes from the minor varieties subjected to survival water conditions displayed an increased presence of benzenoid glycosides. Findings would underscore the adaptability of Moravia Agria and Tinto Fragoso to semiarid environments and suggest their potential to diversify viticulture in the face of a climate change scenario. Therefore, these varieties could contribute positively to the production of new wines, given their aromatic potential.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"350 ","pages":"Article 114283"},"PeriodicalIF":3.9,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Holistic phytoanalysis of salt-stressed Solanum lycopersicum L. enhanced by synergistic hexagonal crystalline ZnO nanoparticles and diazotrophic PGP rhizobacterial consortium","authors":"Najeeba Parre Pakar ,&nbsp;Fazal ur Rehman ,&nbsp;Hassan Javed Chaudhary","doi":"10.1016/j.scienta.2025.114275","DOIUrl":"10.1016/j.scienta.2025.114275","url":null,"abstract":"<div><div>The findings demonstrate a cutting-edge approach to developing elevated salt stress resilience at 900 mM NaCl in <em>Solanum lycopersicum</em> L through synergistic co-inoculation of hexagonal crystalline ZnO nanoparticles (ZnO<img>NPs) and a diazotrophic plant growth-promoting rhizobacterial (PGPR) consortium comprising <em>Pseudomonas aeruginosa</em> PM36, <em>Bacillus</em> Sp. PM37, and <em>Bacillus cereus</em> PM38. The co-inoculation significantly enhanced the synthesis of vital phytohormones, including indole-3-acetic acid, gibberellic acid, and kinetin, under salt stress. These phytohormones are associated with enhanced production of 2,3 DHBA, phosphate solubilization, exopolysaccharide, and ACC deaminase activity. The amplification of genes related to plant growth-promoting (PGP) and stress response, particularly <em>nifH, acdS, pqqE, ituC,</em> and <em>epsA</em>, validates the efficacy of co-inoculating PGPR consortium with ZnO<img>NPs. This synergistic approach markedly improved plant growth, increasing cumulative biomass (45.9 %) and leaf surface area (35.5 %). It also increased photopigments (chlorophyll: 46.94 %, carotenoids: 45.32 %) and osmoprotectants (proline: 15.45 %, proteins: 39.89 %, soluble sugars: 30.19 %, glycine betaine: 24.1 %), all of which are important for osmotic adjustment and stress tolerance. The co-inoculation significantly increased the expression levels of the antioxidant enzymes catalase (49.6 %) and superoxide dismutase (44.1 %) in response to high salt stress. This co-inoculation modulates the activities of antioxidant enzymes to an increase in the inhibitory concentration value of DPPH (32 %), relative water content (32.10 %), alongside decreases in electrolyte leakage (34.6 %), malondialdehyde (76.9 %), and hydrogen peroxide (42.8 %). The results indicate that this combination is highly effective in enhancing reactive oxygen species (ROS)-neutralizing enzymes, specifically ascorbate peroxidase (27.4 %) and peroxidase (41.5 %), which accompany this molecular response. The combined application of PGPR and ZnO<img>NPs improves molecular and enzymatic defense mechanisms while promoting robust growth and physiological performance under high salt stress. This approach presents a promising avenue for developing salt-tolerant crops, thereby contributing to the sustainability of agricultural practices in saline environments.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"354 ","pages":"Article 114275"},"PeriodicalIF":3.9,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing cut Phalaenopsis preservation by the combination of Slightly Acidic Electrolytic Water-based Preservative Solution (SAEW-PS) with Low Voltage Electrostatic Field (LVEF)","authors":"Jiamiao Hu ,&nbsp;Lifang Liu ,&nbsp;Chang-Jer Wu ,&nbsp;Shiqing Zou ,&nbsp;Wanzhen Dai ,&nbsp;Yihui Lu ,&nbsp;Qunhui Dong ,&nbsp;Donghui Peng ,&nbsp;Shaoling Lin","doi":"10.1016/j.scienta.2025.114279","DOIUrl":"10.1016/j.scienta.2025.114279","url":null,"abstract":"<div><div>Cut flowers are highly susceptible to post-harvest quality deterioration due to microbial contamination and dehydration. Therefore, the development of innovative preservation strategies is essential for extending vase life of cut flowers. Here, this study explored the potential of Slightly Acidic Electrolyzed Water-based Preservative Solution (SAEW-PS), in combination with Low Voltage Electrostatic Field (LVEF) treatment, to mitigate senescence in cut <em>Phalaenopsis</em> V3. Firstly, SAEW-PS formulations with varying available chlorine concentrations (ACC) were optimized. Results showed that SAEW-PS containing 60 mg/L ACC significantly extended the vase life by 50 % compared to the control (CK). Subsequently, the combined effects of SAEW-PS and LVEF on mitigating the quality degradation of cut <em>Phalaenopsis</em> were assessed over a 28-d vase period. Results demonstrated that the combined treatment (SAEW-PS + LVEF) markedly improved post-harvest quality, resulting in favorable flower appearance and increased fresh weight (18 %), flower diameter (14 %), and soluble sugar content (114 %). Concurrently, indicators of senescence such as relative conductivity, malondialdehyde (MDA) content, and maximum ethylene release rate were reduced by 35 %, 31 %, and 33 % respectively upon SAEW-PS + LVEF treatment; while activities of antioxidant enzymes (SOD, POD and CAT) were significantly upregulated by 67 %, 34 %, and 44 %, respectively. Furthermore, low-field NMR analysis revealed that SAEW-PS + LVEF treatment effectively suppressed water loss and influenced water migration in the petal of cut <em>Phalaenopsis</em>. Overall, these findings indicated that the integrated application of SAEW-PS and LVEF offers a promising approach to delaying senescence of cut <em>Phalaenopsis</em>, which may also be applicable to other commercially important cut flower species.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"350 ","pages":"Article 114279"},"PeriodicalIF":3.9,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144634510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aluminum stress alters leaf physiology and endophytic bacterial communities in ginseng (Panax ginseng Meyer)","authors":"Chijia Liang ,&nbsp;Fan Zhou ,&nbsp;Guanzhong Ding ,&nbsp;Peng Mu ,&nbsp;Yue Zhang ,&nbsp;Ning Liu","doi":"10.1016/j.scienta.2025.114276","DOIUrl":"10.1016/j.scienta.2025.114276","url":null,"abstract":"<div><div>Aluminum (Al) stress is a significant constraint to plant growth in acidic soils, however, its effects on ginseng (<em>Panax ginseng</em> Meyer) remain poorly understood. This study, investigated the physiological and microbial responses of ginseng to graded Al stress. Ginseng seedlings were grown in sterilized artificial soil and exposed to six concentrations of aluminum sulfate [0 (control), 25, 50, 75, 100, and 200 mM Al₂(SO₄)₃] for 90 days. Growth phenotypes, leaf nutrient contents, photosynthetic parameters, and leaf endophytic bacterial diversity and composition were evaluated to elucidate the mechanisms of Al-induced stress. The study revealed that low Al concentrations (25 and 50 mM) increased leaf fresh weight by 5.78 %–17.33 % and dry weight by 25.93 %–64.49 %, whereas higher concentrations (75, 100, and 200 mM) reduced fresh weight by 5.80 %–18.84 % and dry weight by 20 %–65 %, respectively. As the concentration of Al treatment increased, the leaf Al and iron (Fe) contents increased by 18.00 %–83.15 % and 15.96 %–83.36 %, respectively. Meanwhile, the magnesium (Mg) and phosphorous (P) contents initially increased but then decreased. Chlorophyll content, along with variable fluorescence (Fv) and maximum fluorescence (Fm), initially increased and then decreased as Al concentration rose. The Shannon index peaked at 5.7 under 25 mM Al treatment but decreased to 1.3 under 200 mM Al treatment. At low Al levels, the leaf endophytic bacterial biomarkers were <em>Aeromonas, Bacillus, Shewanella</em>, and <em>Vibrio</em>, whereas <em>Paenibacillus</em> and <em>Ralstonia</em> were associated with high Al concentrations. PLS-PM analysis demonstrated Al showed a significant negative correlation with P, while P exhibited a significant correlation with the photosynthetic indices, as well as the diversity and structure of leaf endophytic bacterial communities. Notably, the genera <em>Pseudomonas</em> and <em>Stenotrophomonas</em> exhibited substantial positive correlations with actual photosynthetic efficiency [Y(II)], and the maximum quantum efficiency of photosystem II (Fv/Fm ratio). The findings demonstrate a concentration-dependent impact of Al on ginseng, with low levels beneficial and high levels detrimental to ginseng growth and physiology. Excessive Al stress impairs photosynthesis by disrupting the diversity of endophytic bacteria. These results underscore the need for soil Al management and the use of beneficial microbes to enhance ginseng resistance, with future efforts directed toward microbial inoculants and molecular strategies for Al-resistant cultivation.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"350 ","pages":"Article 114276"},"PeriodicalIF":3.9,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fertilizer longevity and placement effects on pentas growth and Pennsylvania bittercress control in containers","authors":"Ping Yu ,&nbsp;S. Christopher Marble","doi":"10.1016/j.scienta.2025.114280","DOIUrl":"10.1016/j.scienta.2025.114280","url":null,"abstract":"<div><div>Fertilizers are essential for both crops and weeds, yet the combined effects of longevity and placement have not been studied extensively. Thus, a greenhouse study was conducted to test the effects of fertilizer longevity and placement on pentas (<em>Pentas lanceolata</em>) and Pennsylvania bittercress (<em>Cardamine pensylvanica</em>) growth. Five fertilizer longevity treatments [3-4 month (M), 5-6 M, 8-9 M, 12-14 M] were applied via top-dressing, sub-dressing and incorporating to the substrate. Plant and weed growth were recorded as well as nutrient leaching. The results showed neither fertilizer longevity nor placement had significant impact on pentas growth, yet sub-dressing fertilizer treatments produced the largest bittercress leaf area and shoot dry weight. Moreover, fertilizer longevity and placement both impact nutrient leaching. Overall, in this study, fertilizer placement exerted a greater influence on weed growth than fertilizer longevity.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"350 ","pages":"Article 114280"},"PeriodicalIF":3.9,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144611915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics elucidation of ZnO quantum dots enhancing growth and bioactive compound biosynthesis in Ligusticum chuanxiong Hort","authors":"Mingzhi Zhong ,&nbsp;Fang Peng ,&nbsp;Shan Tao ,&nbsp;Hailang Liao ,&nbsp;Can Yuan ,&nbsp;Xiao Ye ,&nbsp;Yu Wu ,&nbsp;Changqing Mao ,&nbsp;Song Chen ,&nbsp;Wanjing Xu ,&nbsp;Li Liu ,&nbsp;Juan Yang ,&nbsp;Yijuan Kong ,&nbsp;Chao Zhang","doi":"10.1016/j.scienta.2025.114277","DOIUrl":"10.1016/j.scienta.2025.114277","url":null,"abstract":"<div><div>Zinc (Zn) is essential for plant growth and development. In this study, Zn oxide quantum dots (ZnO QDs) were synthesized and applied to <em>Ligusticum chuanxiong</em> seedlings to examine their regulatory effects on growth and bioactive compound biosynthesis. Foliar-applied ZnO QDs exploited nanoscale properties to enhance Zn accumulation, increase photosynthetic pigment levels, and promote carbohydrate and protein biosynthesis. Levels of endogenous phytohormones, including cytokinin (6-benzyladenine, 6-BA) and auxin (indole-3-acetic acid, IAA), were also elevated. ZnO QDs reduced lipid peroxidation through selective activation of catalase and polyphenol oxidase, alleviating oxidative damage. They also enhanced the accumulation of key medicinal compounds: chlorogenic acid, senkyunolide A, ligustilide, and 3-n-butylphthalide. Multi-omics analyses identified two transcriptionally regulated pathways: genes in the tryptophan pathway (<em>TAR4.1, TAR4.2, YUCCA6</em>, and <em>AAO</em>) promoted auxin biosynthesis, whereas genes in the phenylalanine metabolic pathway (<em>PALs, 4CLs</em>, and <em>HCTs</em>) redirected flux toward chlorogenic acid and related metabolites. ERF transcription factors were predominant in regulating both auxin and chlorogenic acid biosynthesis. These findings demonstrate that ZnO QDs coordinate Zn delivery, antioxidant activation, and biosynthesis of medicinal metabolites, supporting a nanotechnology-based strategy for improving the quality and yield of medicinal crops.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"349 ","pages":"Article 114277"},"PeriodicalIF":3.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144596577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Notice to “Melatonin and salicylic acid synergistically improve arsenic induced oxidative stress tolerance in ornamental sword lily” [Scientia Horticulturae, Volume 322, 1 December 2023, 112389]","authors":"Faisal Zulfiqar ,&nbsp;Anam Moosa ,&nbsp;Antonio Ferrante ,&nbsp;Muhammad Nafees ,&nbsp;Anastasios Darras ,&nbsp;Muhammad Mudassir Nazir ,&nbsp;Manal Abdullah AlShaqhaa ,&nbsp;Fahmy Gad Elsaid","doi":"10.1016/j.scienta.2025.114272","DOIUrl":"10.1016/j.scienta.2025.114272","url":null,"abstract":"","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"349 ","pages":"Article 114272"},"PeriodicalIF":3.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144587728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Delayed degradation of tapetal cells causes the male sterility in Lagerstroemia speciosa 'Zichan'","authors":"Ruoyong Yin ,&nbsp;Baojin Zhang ,&nbsp;Shuaijie Lu ,&nbsp;Xugao Sun ,&nbsp;Xiaodi Liu ,&nbsp;Ruchun Xi ,&nbsp;Xiaomei Deng","doi":"10.1016/j.scienta.2025.114261","DOIUrl":"10.1016/j.scienta.2025.114261","url":null,"abstract":"<div><div>The degradation of tapetum and tetrad callose plays crucial roles in the development and maturation of microspores. <em>Lagerstroemia speciosa</em> 'Zichan' is a new cultivar of <em>Lagerstroemia speciosa</em> characterized by pollen sterility and continuous flowering. In this study, abnormal anther development with the delayed degradation of tapetal cells was identified in L. <em>speciosa</em> 'Zichan'. Cytological observation revealed that the degradation of the tapetal cells was delayed after tetrad formation. Histochemistry showed that callose persisted until the early unicellular stage. RT-qPCR analysis indicated that the expression of callose synthase genes was already abnormal at the microsporocyte stage, and the expression level of callose-degrading enzyme-related genes was abnormally low at the tetrad stage. RT-qPCR indicated that as early as the microsporocyte stage, the expression of callose synthase genes in L. <em>speciosa</em> 'Zichan' was already abnormal, and the expression of callose-degrading enzyme-related genes was lowest at the tetrad stage. Taken together, we hypothesize that the delayed degradation of tapetal cells inhibits the release of callose-degrading enzyme at the tetrad stage, further affecting the degradation of callose and microspore release at the right time, finally leading to microspore failure and male sterility in L. <em>speciosa</em> 'Zichan'.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"349 ","pages":"Article 114261"},"PeriodicalIF":3.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144556737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutations in the PGAM1 gene, encoding phosphoglycerate mutase-like protein 1, result in cold tolerance in the tomato plant mutant ‘cr-1′","authors":"Hui-Ru Wang,&nbsp;Jing-Qing He,&nbsp;Xuan Zheng,&nbsp;Yan-Ming Gao,&nbsp;Jia-Yu Wang,&nbsp;Xiao-Min Wang,&nbsp;Jian-She Li,&nbsp;Guo-Xin Cheng","doi":"10.1016/j.scienta.2025.114266","DOIUrl":"10.1016/j.scienta.2025.114266","url":null,"abstract":"<div><div>Cold stress is a major factor limiting tomato productivity. New cold-tolerant varieties must be developed to reduce yield losses. In this study, a cold-tolerant tomato plant mutant ‘<em>cr-1</em>′ previously developed using ethylmethane sulfonate (EMS) was characterized at the physiological, biochemical, and omics levels. The genetic background of ‘<em>cr-1</em>′ (F2 generation) and its wild type (WT) ‘Moneymaker (MM)’ was analyzed, and a key gene controlling the cold tolerance was mapped to the chromosome of the tomato plant mutant ‘<em>cr-1</em>′ using genome-wide association studies (GWAS). Our study revealed that the ‘<em>cr-1</em>′ mutant exhibited mild leaf curling, low hydrogen peroxide (H₂O₂) and superoxide (O₂-) levels, and high antioxidant enzyme activities under cold stress. Combined analysis of the transcriptome and metabolome showed the accumulation of amino acids and their derivatives and stimulation of secondary metabolic pathways such as oxidative phosphorylation, zeatin biosynthesis, and biosynthesis of unsaturated fatty acids in the ‘<em>cr-1</em>′ mutant under cold stress. Further analysis suggested that the ‘<em>cr-1</em>′ mutant has a dominant genetic pattern controlled by a single gene and 5 single nucleotide polymorphism (SNP) mutations on the sixth and eighth exons of the <em>PGAM1</em> (LOC101266024) gene, encoding phosphoglycerate mutase-like protein 1. These observations suggest that <em>PGAM1</em> may be responsible for the mutant's cold tolerance, and the gene mutation probably improves the cold tolerance by promoting sugar accumulation. Thus, the study suggests that the ‘<em>cr-1</em>’ mutant is a valuable resource for breeding and developing novel cold-tolerant tomato cultivars.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"349 ","pages":"Article 114266"},"PeriodicalIF":3.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144519213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}