{"title":"曲霉木霉M7刺激常驻根瘤菌联合体,改变番茄果实代谢谱,提高采后番茄的糖酸比","authors":"Xiaojuan Li, Dengbo Zhou, Junting Feng, Yufeng Chen, Yankun Zhao, Bingyu Cai, Yongzan Wei, Miaoyi Zhang, Dengfeng Qi, Kai Li, Kaibing Zhou, Wei Wang, Jianghui Xie","doi":"10.1016/j.scienta.2025.114366","DOIUrl":null,"url":null,"abstract":"Tomato (<ce:italic>Solanum lycopersicum</ce:italic> L.), a globally cultivated fruit crop of the nightshade family, is highly valued for its broad dietary applications and health-promoting properties. In recent years, escalating consumer preferences for nutritious food options, coupled with rising disposable incomes and heightened awareness of dietary wellness, have significantly driven market demand for superior-grade tomato varieties. Consequently, enhancing fruit quality has emerged as a critical determinant in securing competitive advantages within the agricultural and horticultural sectors. Although using <ce:italic>Trichoderma</ce:italic> to improve fruit quality is an environmentally friendly approach, the microbial ecological mechanisms and the fruit metabolic profile by which <ce:italic>Trichoderma</ce:italic> inoculation affects fruit quality remain unclear. This study primarily explores the role of <ce:italic>Trichoderma asperellum</ce:italic> M7 in improving tomato quality from the perspectives of rhizosphere soil microbial communities and fruit metabolism, revealing its potential mechanisms. It was found that <ce:italic>T. asperellum</ce:italic> M7 enhanced the sugar-acid ratio of tomato fruits through the action of soil microorganisms, and this increase was significantly correlated with the genera <ce:italic>TM7a</ce:italic> and <ce:italic>Candidatus</ce:italic>_<ce:italic>Saccharimonas</ce:italic>. Furthermore, the application of <ce:italic>T. asperellum</ce:italic> M7 was observed to significantly modulate metabolite synthesis pathways in tomato fruits. It notably enhanced the biosynthesis of carbohydrates and organic acid derivatives, with marked increases in specific compounds such as CDP-ribitol, d-glucose, cellobiose, and oxalosuccinate. Furthermore, strain M7 enhanced both the enzyme activity and gene expression associated with sugar metabolism in tomato fruits. Our research findings provided a new perspective on potential strategies for using microorganisms to enhance fruit quality.","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"45 1","pages":""},"PeriodicalIF":4.2000,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Trichoderma asperellum M7 stimulates resident rhizobacterial consortia and alters the tomato fruit metabolic profile to enhance the sugar-acid ratio in postharvest tomatoes\",\"authors\":\"Xiaojuan Li, Dengbo Zhou, Junting Feng, Yufeng Chen, Yankun Zhao, Bingyu Cai, Yongzan Wei, Miaoyi Zhang, Dengfeng Qi, Kai Li, Kaibing Zhou, Wei Wang, Jianghui Xie\",\"doi\":\"10.1016/j.scienta.2025.114366\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Tomato (<ce:italic>Solanum lycopersicum</ce:italic> L.), a globally cultivated fruit crop of the nightshade family, is highly valued for its broad dietary applications and health-promoting properties. In recent years, escalating consumer preferences for nutritious food options, coupled with rising disposable incomes and heightened awareness of dietary wellness, have significantly driven market demand for superior-grade tomato varieties. Consequently, enhancing fruit quality has emerged as a critical determinant in securing competitive advantages within the agricultural and horticultural sectors. Although using <ce:italic>Trichoderma</ce:italic> to improve fruit quality is an environmentally friendly approach, the microbial ecological mechanisms and the fruit metabolic profile by which <ce:italic>Trichoderma</ce:italic> inoculation affects fruit quality remain unclear. This study primarily explores the role of <ce:italic>Trichoderma asperellum</ce:italic> M7 in improving tomato quality from the perspectives of rhizosphere soil microbial communities and fruit metabolism, revealing its potential mechanisms. It was found that <ce:italic>T. asperellum</ce:italic> M7 enhanced the sugar-acid ratio of tomato fruits through the action of soil microorganisms, and this increase was significantly correlated with the genera <ce:italic>TM7a</ce:italic> and <ce:italic>Candidatus</ce:italic>_<ce:italic>Saccharimonas</ce:italic>. Furthermore, the application of <ce:italic>T. asperellum</ce:italic> M7 was observed to significantly modulate metabolite synthesis pathways in tomato fruits. It notably enhanced the biosynthesis of carbohydrates and organic acid derivatives, with marked increases in specific compounds such as CDP-ribitol, d-glucose, cellobiose, and oxalosuccinate. Furthermore, strain M7 enhanced both the enzyme activity and gene expression associated with sugar metabolism in tomato fruits. Our research findings provided a new perspective on potential strategies for using microorganisms to enhance fruit quality.\",\"PeriodicalId\":21679,\"journal\":{\"name\":\"Scientia Horticulturae\",\"volume\":\"45 1\",\"pages\":\"\"},\"PeriodicalIF\":4.2000,\"publicationDate\":\"2025-09-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Scientia Horticulturae\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1016/j.scienta.2025.114366\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"HORTICULTURE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Scientia Horticulturae","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.scienta.2025.114366","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"HORTICULTURE","Score":null,"Total":0}
Trichoderma asperellum M7 stimulates resident rhizobacterial consortia and alters the tomato fruit metabolic profile to enhance the sugar-acid ratio in postharvest tomatoes
Tomato (Solanum lycopersicum L.), a globally cultivated fruit crop of the nightshade family, is highly valued for its broad dietary applications and health-promoting properties. In recent years, escalating consumer preferences for nutritious food options, coupled with rising disposable incomes and heightened awareness of dietary wellness, have significantly driven market demand for superior-grade tomato varieties. Consequently, enhancing fruit quality has emerged as a critical determinant in securing competitive advantages within the agricultural and horticultural sectors. Although using Trichoderma to improve fruit quality is an environmentally friendly approach, the microbial ecological mechanisms and the fruit metabolic profile by which Trichoderma inoculation affects fruit quality remain unclear. This study primarily explores the role of Trichoderma asperellum M7 in improving tomato quality from the perspectives of rhizosphere soil microbial communities and fruit metabolism, revealing its potential mechanisms. It was found that T. asperellum M7 enhanced the sugar-acid ratio of tomato fruits through the action of soil microorganisms, and this increase was significantly correlated with the genera TM7a and Candidatus_Saccharimonas. Furthermore, the application of T. asperellum M7 was observed to significantly modulate metabolite synthesis pathways in tomato fruits. It notably enhanced the biosynthesis of carbohydrates and organic acid derivatives, with marked increases in specific compounds such as CDP-ribitol, d-glucose, cellobiose, and oxalosuccinate. Furthermore, strain M7 enhanced both the enzyme activity and gene expression associated with sugar metabolism in tomato fruits. Our research findings provided a new perspective on potential strategies for using microorganisms to enhance fruit quality.
期刊介绍:
Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.