Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology最新文献

{"title":"COMPARATIVE ASSESSMENT OF THE TURNOVER OF ANTIMICROBIAL VETERINARY MEDICINAL PRODUCTS IN UKRAINE AND EU MEMBER STATES","authors":"Y. Kosenko, N. Ostapiv, L. Zaruma","doi":"10.36359/scivp.2023-24-1.08","DOIUrl":"https://doi.org/10.36359/scivp.2023-24-1.08","url":null,"abstract":"Governments of many countries and professional organizations, including Ukraine, have developed a lot of legislative and regulatory documents and recommendations to tackle the development of acquired antimicrobial resistance, based on the of “One Health” concept. Monitoring the manifestation of acquired antimicrobial resistance and the circulation of antimicrobial veterinary medicinal products is one of the keys to solving this problem. \u0000The main goal of this study was to determine the trends in the sales volumes of antimicrobial veterinary medicinal products and compare them with the turnover of such products in the EU/EEA member states during 2021. \u0000Since 2015, the World Organization for Animal Health (WOAH) has taken the lead to build a global interactive automated database on antimicrobial agents ANIMUSE to facilitate countries' reporting, analysis, and interpretation of data. In 2022, Ukraine, like other countries, for the first time submitted an annual sales volumes report of antimicrobial veterinary medicinal products online to the global ANIMUSE database on the antimicrobials intended for use in animals (AMU). \u0000As a result of the sales volumes reports of antimicrobial veterinary medicinal products analysis for 2021, common trends were revealed in Ukraine and EU/EEA member states regarding the circulation of antimicrobial veterinary medicinal products of category D, in particular tetracyclines and penicillins. However, was established an increase in the sales share of aminoglycosides (7.93 %), polymyxins (8.5 %) and fluoroquinolones (10.4 %), and the 3rd and 4th generation cephalosporins (0.72 %) in Ukraine, which are classified as categories C and B, the use of which must be reasonable in the absence of alternatives from category D. \u0000Monitoring data of sales volumes does not allow us to assert that all of the sold antimicrobials were used during the reporting period, however, the increase in their share in sales volumes is worrisome following the requirements stated by the “Procedure for the use of antimicrobial veterinary medicinal products”, approved by order of the Ministry of Economy of Ukraine No. 1177-21 of 12.30.2021.","PeriodicalId":21617,"journal":{"name":"Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology","volume":"78 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88338440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE EFFECT OF AROMATASES INHIBITOR-BASED PRODUCTS ON COW REPRODUCTIVE ABILITY","authors":"O. Chekan","doi":"10.36359/scivp.2023-24-1.28","DOIUrl":"https://doi.org/10.36359/scivp.2023-24-1.28","url":null,"abstract":"Mycotoxin zearalenone is a phytoestrogenic substance that can disrupt the functioning of the organs of the reproductive system of cows. At the same time, a hyperestrogenic state occurs, which \u0000leads to overeating, multiple unsuccessful inseminations and infertility of cows. \u0000The purpose of the research is to investigate the effect of a single application of drugs based on aromatase inhibitors on the main biochemical and hormonal parameters of the blood of cows, as well as to establish their effectiveness during spontaneous expression of desire and when applying synchronization schemes. \u0000The effect of a single dose of aromatase inhibitors on the dynamics of sex hormones in cows, which directly affect the formation of the dominant follicle and its ovulation, was investigated. At the same time, a 1.56-fold decrease in estradiol, a 1.67-fold increase in progesterone, a 1.29-fold increase in luteinizing hormone, and a 1.76-fold increase in follicle-stimulating hormone were found. At the same time, the maturation of the dominant follicle and its ovulation at a size of 22 to 24 mm have been established. A change in the biochemical parameters of the blood serum of cows after the use of products based on an aromatase inhibitor was revealed. A tendency was established to restore the level of calcium to 2.01±0.27 mmol/l, phosphorus – 1.1±0.41 mmol/l, magnesium – 0.87±0.11 mmol/l, selenium –1.0± 0.1 mmol/l. At the same time, the level of creatinine decreased by 1.2 times compared to sick animals, the indicators of liver transferases also tended to decrease. A significant decrease in the blood urea index by 1.69 times was established, which indicates the restoration of protein and lipid metabolism. \u0000The indicators of fertilization of cows with spontaneous manifestation of sexual desire when using agents based on aromatase inhibitors were studied. At the same time, the fertility rate ranged from 69.39% in cows older than 5 years to 80.39% in cows after the 1st calving. \u0000A positive effect of aromatase inhibitors, introduced before the use of estrus synchronization schemes in cows, was established on the development of the dominant follicle, luteogenesis and the appearance of a new wave of follicle development. Fertilization averaged from 68.1% to 76.42%.","PeriodicalId":21617,"journal":{"name":"Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology","volume":"88 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76392182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DEVELOPMENT AND VALIDATION OF HPLC-METHOD FOR SIMULTANEOUS QUANTITATIVE DETERMINATION OF IMIDACLOPRID AND IVERMECTIN IN DROPS AGAINST FLEAS AND TICKS","authors":"R. Ostapiv, V. Tkachenko, S. L. Humeniuk, L. Samarska","doi":"10.36359/scivp.2023-24-1.16","DOIUrl":"https://doi.org/10.36359/scivp.2023-24-1.16","url":null,"abstract":"Imidacloprid and ivermectin are selective blockers of metabotropic ion receptors of the nervous system of invertebrates, leading to paralysis and death of ecto- and endoparasites. These active substances are used in insect-acaricidal drops to kill lice, fleas and ticks in cattle, domestic animals and pets. \u0000The aim of the work was to develop a method for the identification and simultaneous quantitative determination of imidacloprid and ivermectin in drops for external use. The method was developed and validated by selectivity, linearity and suitability parameters of the chromatographic system. Drops for external use were used as a sample-object for development. The standard sample and the test sample were dissolved in acetonitrile to a concentration of imidacloprid 100 μg/ml and ivermectin 10 µg/ml. The samples were separated on a Dionex Ultimate 3000 chromatography system equipped with a chromatographic column Acclaim C18 150×4.6, 5 μm. The mobile phase was a mixture of acetonitrile and water in a volume ratio of 90:10. Ivermectin and imidacloprid were detected at an absorption wavelength of 242 nm. \u0000Under the above-mentioned conditions, it was possible to completely separate imidacloprid and ivermectin (retention time of chromatographic peaks – 2.0 min and 10.0 min) and other components of the studied drug. At the same time, the suitability parameters of the chromatographic system did not exceed the limits specified in the recommendations of the USA Food and Drug Association. For the peaks of imidacloprid and ivermectin, the efficiency of the chromatographic system was 8000–10000 theoretical plates. The relative standard deviation (RSD) for the peak areas of the active substances was ± 0.31%, and the peak separation ratio (RS) of imidacloprid from ivermectin and other drug components was 35.9. The symmetry coefficient of the imidacloprid peak was 1.5, and that of ivermectin was 1.1. The calibration curves were linear in the recommended DFU 2.0 range (80–120% of the nominal concentration of the corresponding active substance). The coefficient of linearity (R2) for imidacloprid was 0.9991, and for ivermectin it was 0.9993.","PeriodicalId":21617,"journal":{"name":"Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology","volume":"92 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74014809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"INFLUENCE OF THE PREPARATION BASED ON BUTAPHOSPHANE AND CYANOCOBALAMIN ON THE PHYSIOLOGICAL AND FUNCTIONAL STATE OF THE ORGANISM OF WEANLED PIGLETS","authors":"Основі Бутафосфану, І Ціанокобаламіну, НА ФІЗІОЛОГО-ФУНКЦІОНАЛЬНИЙ, Стан Організму, Відлучених Поросят, N. Lisova, O. Pyatnychko, T. Stetsko, N. Shkodyak, O. Sobodosh, O. Maksymovych, O. Myhalyuk","doi":"10.36359/scivp.2023-24-1.10","DOIUrl":"https://doi.org/10.36359/scivp.2023-24-1.10","url":null,"abstract":"The article presents the results of clinical trials of a veterinary medicinal product based on butaphosphane and cyanocobalamin on weaned piglets during the growing period with signs of anemia. The drug was used in the dosage recommended by the manufacturer - intramuscularly in a dose of 2.0 ml per animal, once a day, for 5 days. \u0000Blood for laboratory research was taken from animals before and after the use of the drug. Research of hematological and biochemical parameters was carried out according to generally accepted methods. The therapeutic effectiveness of the drug was evaluated by comparing it with the data obtained before the start of treatment. Based on the obtained data and their statistical analysis, conclusions were made about the effect of the drug on the morpho-functional state of the piglets' body. \u0000A comparative analysis of hematological indicators of piglets before and after the use of the drug showed optimization of the leukogram, which was a sign of improvement in the physiological condition of the animals. Activation of hematopoietic processes was noted, confirmed by indicators of red blood of piglets. \u0000The biochemical profile of the blood serum of experimental animals after treatment showed a positive effect on indicators of mineral and protein metabolism and the state of the hepatobiliary system. \u0000Based on the assessment of the clinical condition of the animals and the obtained results of laboratory studies, it was established that the tolerability of the studied drug when administered to piglets with signs of anemia was good, no changes in the behavior of the piglets were detected. Side effects and negative phenomena have not been established. Monthly monitoring of the clinical condition of piglets confirmed the safety of the drug when used in the dosage and method of administration recommended by the manufacturer. The results of hematological and biochemical studies confirmed the positive effect of the drug on the body and its effectiveness in the treatment of the anemic state of piglets and the correction of the physiological state of piglets.","PeriodicalId":21617,"journal":{"name":"Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology","volume":"382 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74262255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GROWING OF ORGANIC FISH IS IN NATURAL POND","authors":"N. Pustova, Z. Pustova, D. Balickiy","doi":"10.36359/scivp.2023-24-1.20","DOIUrl":"https://doi.org/10.36359/scivp.2023-24-1.20","url":null,"abstract":"The ponds of natural zones is economic activity on the most variety’s in limit, a sent to maximal of ecosystem maintenance – adherent territory to the pond and variety of flora and fauna. To organization of organic fish-farms on the ponds is natural zones creates the swift reduction to the prime price of fish is for a sale and improvement of basic fish economic indexes. \u0000  \u0000Researches our methodology is envisaged the study of features are growing and productivity of fish in three types: Сarpio, Ctenopharyngodon idella, Hypophthalmichthys in the conditions of natural pond at the organic growing of fish-farming. \u0000Feeding of the Сarpio in a natural pond at the organic growing of fish-farming was begun, when the temperature of water attained +10˚С. For the gradual getting is a fish used to the feed, in places laid into forage out is feed small portions for 2-3 kg. The feeding was distributed once for twenty-four hours of morning oh a 6-7 o'clock, that fish of him used to 12 hour, and in the second half of day she consumed natural forage. The important index of working out of fish is a rate of height, most intensive grew and the greatest average daily increases marked for fishes in the summer months of from 4 to 15 g on a head for twenty-four hours. At the beginning of growing of the Сarpio is consumption of forage on a head for twenty-four hours, were within the limits of a 4-5 g, and maximal a 7 g, showed up in August, when increase under yearling is mainly arrived at due to feeding of forage. \u0000The economic indicators of growing of fishes testify at the terms of natural pond and of organic fish-farms, about the greatest profitability is the Hypophthalmichthys – the 61 percent, in a subzero the Сarpio – 42 percent and on three types of fishes – 52,67 percent. Complex intensification of growing of fish is the scientifically reasonable system of economies of resources and money, is the mortgage of efficient of the economy of organic fish-farms.","PeriodicalId":21617,"journal":{"name":"Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81544732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"POTENTIATION OF PROBIOTIC ACTIVITY BY SIMULTANEOUS USE OF AEROCOCCUS VIRIDANS AND MYCOBACTERIUM VACCAE","authors":"I. Biben, O. I. Sosnytskyi, V. Zazharskyi, A. O. Sosnytska, N. G. Useeva","doi":"10.36359/scivp.2023-24-1.02","DOIUrl":"https://doi.org/10.36359/scivp.2023-24-1.02","url":null,"abstract":"In modern animal husbandry, progressive intensive technologies for keeping animals and the productive exploitation of animals are economically efficient, but they deplete the physiological reserves of the body and lead to undesirable pathophysiological changes of a complex nature. The immune-reactive potential of the integral and censoring systems of the macroorganism decreases, the metabolic activity of enzyme complexes at the level of cells and organs changes, antimicrobial resistance decreases, and the quantitative and qualitative composition of the normal flora of the covering tissues and hollow organs is disturbed. The stress of debilitating technological leads to systemic pathologies of infectious and non-infectious origin and deterioration of the health and well-being of the animals. \u0000One of the physiological ways of correcting the systemic pathology based on damage to the native bioprotective mechanisms of the macroorganism is the use of resident prokaryotes of the microbiome artificially cultivated and given per os as a feed dietary supplement. In our research, we isolated probiotic cultures of A. viridans and M. vaccae from healthy broiler chickens, studied their biological properties, established their biosafety for other animals, and determined probiotic potencies when used monovalently and in association. It was shown that the combined use of probiotic prokaryotes significantly improves the stimulation of the immune-reactive potential of the macroorganism and, in association, increases the colonization capabilities of indigenous prokaryotes. Isolated probiotic cultures of the resident microbiota are suitable for the production of probiotics and symbiotics for farm and domestic animals and for prophylactic and therapeutic use both in monoculture and in association. Simultaneous use of probiotic associations is more effective than monocultures.","PeriodicalId":21617,"journal":{"name":"Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85678728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DETERMINATION OF THE CHRONIC TOXICITY PARAMETERS OF THE COMPLEX REMEDY FOR THE TREATMENT OF CYSTICERCOSIS IN RABBITS","authors":"М. В. Богач, вет, професор Л. В наук, Пероцька, доцент Д. М. Богач канд. вет. наук, О філософіїО., Горобей","doi":"10.36359/scivp.2023-24-1.03","DOIUrl":"https://doi.org/10.36359/scivp.2023-24-1.03","url":null,"abstract":"The article presents the data of a preclinical test of a means for the treatment of cysticercosis in rabbits by intra-gastric administration to white mice. Cysticercus pisiformis is a disease caused by ingestion of eggs of Taenia pisiformis in food or water, a tapeworm that parasitizes the small intestine of dogs and other carnivores. As a result of the invasion, the body loses its ability to fully function, the resistance of the immune system to infectious diseases decreases. In order to avoid the spread of the disease, it is necessary to make a timely diagnosis. The obtained data can be used to take the necessary measures against infestations and are important to prevent the selective use of anti-parasitic agents, which can lead to anthelmintic resistance. \u0000The purpose of the work was to determine the parameters of chronic toxicity of the developed complex anti-parasitic agent for the treatment of rabbits for cysticercosis on white mice under experimental conditions. \u0000The animals of the experimental groups were given an aqueous mixture of 0.0002 dm3 of the drug containing the following doses of the drug daily for 12 days: 1/100 DL50 ‒ 16.80 mg/kg (I group); 1/50 DL50 ‒ 33.58 mg/kg (II group); 1/25 DL50 ‒ 67.18 mg/kg (III group). On the next day after the last administration of the drug, on mice, under the condition of light ether narcosis, blood samples were taken for morphological and biochemical studies. \u0000The agent for the treatment of cysticercosis in rabbits, at doses of 1/50 DL50 and 1/25 DL50, resulted in a slight decrease in hemoglobin content and erythrocyte count compared to the control, but it did not suppress bone marrow erythropoiesis in laboratory animals. A decrease in the number of lymphocytes and a slight increase in the level of neutrophils were recorded, which indicates the irritating effect of increased doses of the active substance of the drug. According to the classification of chemical substances by degree of danger (DST 12.1.007–76), it belongs to the class of \"moderately toxic substances\".","PeriodicalId":21617,"journal":{"name":"Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88725406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EFFECTIVENESS OF INFLUENCE OF MINERAL PHYTOBIOTIC ADDITIVE ON AGE DYNAMICS OF LINEAR GROWTH OF SHEEP","authors":"M. Perih","doi":"10.36359/scivp.2023-24-1.19","DOIUrl":"https://doi.org/10.36359/scivp.2023-24-1.19","url":null,"abstract":"The article presents the results of research on the linear growth of young sheep grown on diets enriched with a mineral phytobiotic additive. Indexes of body structure have been determined, which make it possible to establish the relative development of individual parts of the animals’ body. Body structure indexes characterize the sex, age and constitutional characteristics of animals and their typical differences and have a great importance for the animals characteristic. \u0000In order to determine certain patterns of linear growth, coefficients of variability were calculated. \u0000The study of the linear growth of control and experimental animals in our experiments allowed us to reveal the peculiarities of the body structure of the best constitutional types, sexual characteristics, productivity nature and evaluate the principles of the formation of meat qualities of the sheep. In particular, it was established that the growth rate of live mass is accompanied by significant changes in the axial skeleton. \u0000The height measurements and body length of experimental sheep and rams were higher than the control ones accordingly on 4,30-5,92 % and 5,52-5,28 %. The body length of the experimental ones were higher on 6,05 % in sheep and 6,76 % in rams. When determining the width and depth of the chest and the width at the hips, the difference between the control and experimental ones was 13,00-13,10 %, and these indicators increased with age. \u0000The determination of volumetric measurements proves a more stable regularity of age changes. According to the girth of the chest behind the shoulder blades, the experimental sheep exceeded the control ones on 10,96-13,73 %, and the rams on 7,37-12,14 %. \u0000In the rams, compared to the sheep, both in the control and in the experimental ones, the diameters of the parts of the body were higher. They were characterized by a higher height, long enough, wide and deep body and well-defined meat forms of the exterior. \u0000Therefore, our research revealed that according to the parameters of linear growth, the young animals of the experimental group significantly exceeded the animals of the control group of sheep. These advantages are characterized in detail the indexes of body structure, which emphasize the relative proportionality and compactness of individual parts of the body and reflect important productive qualities of animals, especially the meat qualities. \u0000Age-related changes experience the indexes in stretch, chest indexes, and body mass development. In particular, the sheep of the experimental group had an advantage over animals of the control group of 9,81-10,3 7 %. The massiveness index according to scientist Durst experienced more significant age changes between experimental and control young sheep.","PeriodicalId":21617,"journal":{"name":"Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81652744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EXPERIENCE OF USING THE IMMUNE-COMPLEX VACCINE PULVAK MAGNIPLEX IN THE PREVENTION OF INFECTIOUS BURSA DISEASE OF BROILERS","authors":"I. K. Avdosieva, O. Basarab, O. I. Chaikovska, Yu. P. Rudyk","doi":"10.36359/scivp.2023-24-1.01","DOIUrl":"https://doi.org/10.36359/scivp.2023-24-1.01","url":null,"abstract":"The article presents the results of serological control of the effectiveness of vaccination of broilers against infectious bursal disease (IBD) when the immune-complex vaccine Pulvak MagniPlex is used in the incubator. \u0000Immune-complex vaccines are vaccines of a new generation, the use of which completely eliminates post-vaccination complications in birds and interference phenomena. The vaccine includes a mixture of specific antibodies and the vaccine strain of the IBH virus. The main property of these vaccines is that when administered subcutaneously in day-old chicks or 18-day-old chicken embryos by the in ovo method, immunity is quickly created even in the presence of high maternal antibodies (MAB). Vaccination in the incubator using immune-complex vaccines is more accurate and provides protection to the whole bird, unlike live vaccines, the main method of which is administration with drinking water. Immuno-complex vaccines are effective even in the presence of high MAT in chickens. Due to this, they provide a much higher level of protection due to the high homogeneity of protective antibodies compared to live attenuated vaccines. \u0000We present the data of serological monitoring on the average titer of antibodies to the IBH virus in blood sera from 15 batches of broilers aged 45-50 days, which were immunized on the first day in the incubator with the immune-complex vaccine Pulvak MagniPlex. It was established that during a single vaccination in the incubator (15 batches), the Pulvak MagniPlex vaccine stimulates the formation of an active immune response to the IBH virus for the entire period of fattening of broilers. The average titers in the blood sera of broilers aged 45-50 days to the IBH virus ranged from 6,995 to 12,388 after a single vaccination in the incubator (15 batches). The percentage of protective antibodies to the IBH virus in 15 batches of broilers varied from 77.3 to 100, which indicates the intensity of immunity to IBH. A single application of the immune-complex vaccine Pulvak MagniPlex in the incubator stimulates the formation of an active immune response to the IBD virus for the entire period of broiler fattening and provides 100 % protection of 12 batches of broilers and 77.3 % of broilers of 3 batches to IBD. The use of Pulvak MagniPlex allows: to create stable immunity against IBD; to form immunity in each individual broiler, regardless of the level of maternal antibodies; to reduce the impact of stress (compared to mass vaccination in the poultry house by the drinking method. Constant control of the effectiveness of the Pulvak MagniPlex immune-complex vaccine by means of serological monitoring of blood sera in ELISA will allow, depending on the epizootic situation, to timely adjust the vaccination scheme of poultry against IBD.","PeriodicalId":21617,"journal":{"name":"Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology","volume":"65 6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76043717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BIOCHEMICAL AND CHEMICAL COMPOSITION OF VERMICULTURE BIOMASS GROWN ON POULTRY LITTER FERMENTED BY THE ACCELERATED METHOD","authors":"I. S. Osipenko, S. Merzlov","doi":"10.36359/scivp.2023-24-1.15","DOIUrl":"https://doi.org/10.36359/scivp.2023-24-1.15","url":null,"abstract":"The intensive increase in the number of meat poultry, directly broiler chickens both in our country and in the most countries of the world, is accompanied by the accumulation of a large amount of waste (droppings with litter) of this technology in limited areas. According to a number of researchers, the utilization of this biomass must take into account environmental requirements and challenges. These requirements are met by the utilization of poultry droppings by means of vermiculture (growing worms). It is promising to prepare the droppings of broiler chickens with bedding for its use as a substrate for vermiculture by composting with the participation of biodestructors (microbiological preparations). However, the problem of the influence of poultry droppings in the composition of the substrate fermented with the help of biodestrutor BTU-CENTER on the chemical composition of vermiculture biomass remains unstudied. The material for study was the biomass of worms grown on a substrate containing broiler droppings fermented with various doses of a biodestructor. In the control group, vermiculture biomass was grown on a substrate that included 92.0% by weight of broiler chicken droppings with litter fermented for 540 days without the use of a biodestructor and 8.0% wheat straw. In the 1st, 2nd, and 3rd experimental groups, worms were grown on a substrate consisting of 92.0% by mass of broiler chicken droppings with fermented litter for 180 days using the BTU-CENTER biodestructor, respectively, in the amount of 3.75, 7 .5 and 11.25 cm3/t and 8.0% wheat straw. The content of total protein, lipids, ash, zinc, ferrum, copper, lead and cadmium was determined in vermiculture biomass. It has been proven that the content of total protein in vermiculture biomass grown on broiler chicken droppings fermented with a low dose of biodestructor almost does not change. In the biomass of worms obtained on a substrate with the content of broiler droppings fermented with a biodestructor in the amount of 11.25 cm3/t, the total protein content increases by 2.1% compared to the control one. A statistically significant increase in the content of lipids in the biomass of vermiculture grown on a substrate containing broiler droppings fermented with the highest dose of biodestructor (experimental group III) was established. The difference with the control group was 6.7%. It was proven that the more biodestructor was used for fermentation of poultry droppings, the higher the content of total ash was found in the vermiculture biomass grown on it, comparing the I, II and III research groups. The highest ash content was found in the control one. The difference with the experimental groups was 18.1, 12.1 and 6.0%, respectively. Using the substrate with the content of manure fermented with a biodestructor in doses of 7.5 and 11.25 cm3/t, the content of ferrum in the vermiculture biomass was lower than in the control group by 1.95 and 0.76%, respectively. The highest content of cop","PeriodicalId":21617,"journal":{"name":"Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91380210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}