Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology最新文献

{"title":"DEVELOPMENT AND VALIDATION OF HPLC-METHOD DETERMINING QUANTITATIVE CONTENT OF CEFTIOFUR HYDROCHLORIDE IN SUSPENSIONS FOR INJECTION","authors":"R. Ostapiv, V. Tkachenko, S. Y. Chipak","doi":"10.36359/scivp.2022-23-1.17","DOIUrl":"https://doi.org/10.36359/scivp.2022-23-1.17","url":null,"abstract":"Ceftiofur is a third-generation cephalosporin used in veterinary medicine to treat infections caused by gram-negative and gram-positive bacteria. It is most often used in the form of suspensions for injections based on a lipid matrix, since the active substance is poorly soluble in water. The analysis of such a drug by direct spectrophotometry is difficult due to the components of the matrix, therefore it is proposed to develop a high-performance liquid chromatography method with UV detection. \u0000The aim of the work was to develop a method of identification and quantitative determination of ceftiofur hydrochloride in suspensions for injections. The method was developed and validated according to the indicators of selectivity, robustness, linearity and suitability parameters of the chromatographic system. Suspension for injections containing ceftiofur 50 mg/ml was used as a sample-object for method development. The standard sample and the test sample were dissolved in the mobile phase to a concentration of 50 μg/ml. The total uncertainty of the analysis was 1.62%, which is within the limits recommended in DFU 2.0. The samples were separated on a Dionex Ultimate 3000 chromatograph equipped with a Kinetex C18-XB 150×4.6, 5 μm chromatographic column. The mobile phase was a mixture of acetonitrile and 0.05 M ammonium acetate, 0.01 M tetrabutylammonium bromide with a pH of 6.8, titrated with acetic acid, in a volume ratio of 3:7. Ceftiofur hydrochloride was detected spectrophotometrically at a wavelength of 290 nm. \u0000Under the above conditions, it was possible to completely separate ceftiofur (retention time of the chromatographic peak – 4.4 min) and other components of the studied drug. At the same time, the suitability parameters of the chromatographic system did not exceed the limits specified in the recommendations of the USA Food and Drug Association. For the ceftiofur hydrochloride peak, the efficiency of the chromatographic system was 13,900 theoretical plates. The relative standard deviation (RSD) for the peak areas of the active substance was ±0.11 %, and the peak separation coefficient (RS) of ceftiofur hydrochloride from other components of the drug was 17.3. The symmetry coefficient of the peak was 1.02. The calibration curves were linear in the recommended DFU 2.0 range (80–120% of the nominal concentration of the corresponding active substance). The coefficient of linearity (R2) for the peak area of ceftiofur hydrochloride was 0.9992.","PeriodicalId":21617,"journal":{"name":"Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87072762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HISTOLOGICAL CHANGES IN EXPERIMENTAL KERATITIS IN RABBITS AGAINST THE BACKGROUND OF STEM CELL ADMINISTRATION","authors":"T. L. Savchuk, R. Bokotko, O. Shupyk, L. Kladnytska","doi":"10.36359/scivp.2022-23-1.21","DOIUrl":"https://doi.org/10.36359/scivp.2022-23-1.21","url":null,"abstract":"The article highlights the results of histological research of experimental keratitis in rabbits after administration of allogeneic mesenchymal stem cells. These research results provide an opportunity for further study of the effect of allogeneic mesenchymal stem cells on clinical cases of keratitis in animals. \u0000A decrease in the function of the eye or its biological activity at the tissue level leads to the development of a serious eye disease - keratitis. The causes of keratitis are acute and chronic inflammatory processes in the parenchyma of the eye, ionizing radiation, degenerative changes, surgical operations, and others. Clinical manifestations of keratitis vary from redness to destructive damage of eye tissues. The use of complex stimulating therapy often smoothes the clinical picture of keratitis and for a short time stops inflammatory processes in the eye, which can later lead to a latent state of the eye. All this indicates that it is necessary to investigate the use of stem cells in eye diseases in animals. \u0000Our histological studies have shown that the regeneration of eye tissues with injected allogeneic mesenchymal stem cells is more effective means of treating keratitis than the traditional method of treatment (daily instillation of gentaline 0.4% and cypronorm 4-6 times a day). Specifically, stem cells act as a regulator of proliferation in damaged tissues, activate the synthesis of anti-inflammatory mediators and strengthen their own antioxidant properties. \u0000It was established that with the help of allogeneic mesenchymal stem cells, the differentiation of epithelial cells in the layers of the eye and a slight delamination of collagen fibrils were noted already on the 7th day, and on the 14th day the epithelial layer approached normal and there was no inflammatory infiltration of the cornea. Also, on the 30th day of the experiment, almost complete restoration of the structures of the damaged tissue of the eye and the end of the inflammatory process had already taken place. That is, histological studies show that allogeneic mesenchymal stem cells not only restore the function of damaged tissue structures, but also affect the intensity of the inflammatory process, which significantly reduces the time of eye tissue repair at the level of cells and tissues. The obtained data using stem cells can be used for new modern effective methods of treatment of eye pathologies.","PeriodicalId":21617,"journal":{"name":"Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84618906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MORPHOLOGICAL INDICATORS OF BLOOD IN CATS WITH CALICIVIROSIS","authors":"N. Radzіkhovskyі, R. Sachuk, I. Sokulsky, О. Dyshkant, O. Tolokevich","doi":"10.36359/scivp.2022-23-1.20","DOIUrl":"https://doi.org/10.36359/scivp.2022-23-1.20","url":null,"abstract":"In recent years, there has been a massive trend in Ukraine to keep cats, which has inevitably led to an increase in the incidence of their diseases. In the general pathology of cats, viral diseases occupy a leading place, and of particular concern is calicivirosis, which is caused by a virus capable of high mutation rates and can cause atypical course with nonspecific manifestations of clinical signs. Therefore, given the urgency of this issue, the result of our research is to clarify, supplement and summarize data on hematological and biochemical parameters of blood and erythrocytopoiesis in calicivirosis in cats, because blood is a sensitive and informative indicator of the body, responds quickly to changes as exogenous. and endogenous factors. The research was conducted in veterinary clinics of Zhytomyr and Kyiv, on purebred and outbred cats (n = 18). Diagnostic studies to confirm feline calicevirus were performed using rapid tests Ag Test (FCV Ag), Quicking Biotech Co., Ltd. and ELISA and PCR for the presence of calicivirus antigen on the basis of a private veterinary laboratory LLC \"Bald\" in Kiev. \u0000Comprehensive studies have shown that cats with calicivirosis (18) are characterized by changes in morphological and biochemical composition of blood. Deviations from physiological limits were found, namely erythrocytopenia P≤0.05, thrombocytosis P≤0.05, leukocytosis P≤0.05, monocytosis P≤0.01, increase in ESR P≤0.001 and lymphocytopenia P≤0.05, for biochemical ones it is a significant increase in the concentration of globulin P≤0.001, creatinine P≤0.001, urea P≤0.05 hyperenzymemia α-amylase P≤0.001, and ALT P≤0.05. As a result of experimental research of morphological indicators of blood the difficult pathogenesis of a disease with multiorgan insufficiency is established.","PeriodicalId":21617,"journal":{"name":"Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88584919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ENDOPARASITES OF VIETNAMESE BELLIED PIGS IN THE PASTURE GROWING SYSTEM IN FARMS OF THE ODESA REGION","authors":"М. В. Богач, вет, професор О. М наук, Богач, М. Bogach, О. Bohach","doi":"10.36359/scivp.2022-23-1.04","DOIUrl":"https://doi.org/10.36359/scivp.2022-23-1.04","url":null,"abstract":"The article provides data on the spread of endoparasitoses in vietnamese bellied pigs in the pasture growing system in the farms of the Odesa region. Pigs can have numerous intestinal parasites, most often protozoa and nematodes. Although the course of such parasitic infections is usually subclinical, clinical manifestations may occasionally occur, especially in growing pigs. The study of the structure and functioning of parasitic systems is extremely important. The obtained data on gastrointestinal parasites in pigs can help in the development of effective and sustainable measures for the prevention of pig diseases. \u0000The aim of the work was to monitor endoparasitoses in vietnamese bellied pigs in the pasture growing system, depending on age, in the farms of the Odesa region. \u0000A total of 475 samples of feces of vietnamese bellied pigs from 6 private farms of Odesa region were studied. Fecal samples were collected from suckling piglets and weanling piglets (<4 months), young (4‒8 months) and adults (>8 months) of both sexes. \u0000It was established that the total infestation with protozoa in piglets < 4 months. age was 86.7 %, in growing pigs (4-8 months) ‒ 28.9 %, and in adults ‒ 18.7 %, helminthes infestation was 13.3 %, 71.1% and 81.3% in accordance. Piglets < 4 months. age, Isospora suis (28.3 %) and Eimeria suis (18.6 %) were most infested, while Strongyloides sp. (2.7 %). \u0000Ascaris suum (33.4 %) and Trichuris suis (24.6 %) had the highest lesion extent in growing pigs (4‒8 months), and the lowest among protozoans was Isospora suis and Blastocystis sp. (2.9 %). In adult pigs, the highest infestation of Ascaris suum (34.4 %) and Trichuris suis (31.2 %) was recorded, while isosporosis and cryptosporidiosis were not recorded.","PeriodicalId":21617,"journal":{"name":"Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74867357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EFFECT OF NUTRIENTS OF FEED PREPARED WITH PROBIOTIC ON MILK QUALITY AND COW PRODUCTIVITY IN THE FOREST-STEPPE ZONE OF THE CARPATIAN REGION","authors":"N. Fedak, G. Sedilo, S. Chumachenko, I. Dushara","doi":"10.36359/scivp.2022-23-1.25","DOIUrl":"https://doi.org/10.36359/scivp.2022-23-1.25","url":null,"abstract":"The purpose of the research was to determine the effect of feed nutrients, in particular, silage prepared from a wheat-oat mixture with the use of the probiotic preparation kt-l 18/1 (selection of the probiotics laboratory of the institute of agricultural microbiology and apv) in a dose of 8.0 ml of suspension per 1 ton of green mass on the fractional composition of milk proteins and its qualityand on the productivity of cows in the forest-steppe zone of the Carpathian region. \u0000The research was carried out on two groups of lactating cows of the Ukrainian black-spotted breed, similar in age, live weight and milk yield for the previous lactation, 10 cows each. In each the animals of both groups received the basic diet, in addition to which the cows of the control group were fed 20 kg of wheat-oat silage prepared on the farm according to traditional technology, and the experimental group - 19 kg of silage preserved with the use of the probiotic KT-l 18/1. \u0000It was established that the use of probiotics for harvesting silage contributed to obtaining a high-quality product, the introduction of which into the rations of cows in the transition period made it possible to minimize the deficit of dry matter (from 19.8 to 9.1%) and protein (from 4.2% to2.1%), and in winter – from 28.0 to 8.5% in terms of dry matter and from 10.1 to 0% in terms of protein, respectively. \u0000The study of the chemical composition showed an increase in the content of dry matter, mainly due to the total protein, fat and casein in the milk of experimental cows both in the transitional and stable periods of retention \u0000The milk of the cows of the research group during the stable period of storage contained more dry matter, fat, total protein and casein, respectively, by 4.8; 3,4; 5.2 and 5.6% than the control, which led to an increase in its density by 0.7 оА. \u0000In the milk of experimental cows, the improvement of parameters affecting its syrupiness was noted: casein content, density. According to both the reductase test and the fermentation test, which indicates the presence of gas-forming microflora in the milk and its syrupy quality, the milk of the control cows was assigned to the ІІ, and the experimental cows to the І quality class. \u0000The average daily yield of natural milk for 45 days of the accounting period in the experimental group was 16.7 kg and was 5.7% higher than in the control group (15.8 kg).","PeriodicalId":21617,"journal":{"name":"Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84124471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DOXYCYCLINE EFFICIENCY AND SAFETY IN THE TREATMENT OF ACUTE INTESTINAL INFECTIONS OF BACTERIAL ETIOLOGY IN BROILER CHICKENS","authors":"N. Shkodyak, T. Stetsko, O. Pyatnychko, O. Sobodosh, N. Lisova, L. Ostrovska, O. Balyan","doi":"10.36359/scivp.2022-23-1.27","DOIUrl":"https://doi.org/10.36359/scivp.2022-23-1.27","url":null,"abstract":"Doxycycline is a bacteriostatic antibiotic from the group of tetracyclines, a semi-synthetic derivative of chloretracycline. It is a broad-spectrum antibiotic that is active in gram- positive and gram- negative bacteria, including some anaerobes. Therefore, the use of doxycycline drugs is relevant to solve the problem of treatment and control of mono- and polyinfections, which are caused by doxycycline sensitive and other antibiotics resistant to other antibiotics. \u0000The article presents the results of clinical studies of a new veterinary drug Doxal (water-soluble powder for oral administration), production of PJSC “Technologist” (Ukraine), an active pharmaceutical ingredient of which is doxycycline, in the treatment of acute intestinal disease in broiler chickens. \u0000A high level of therapeutic efficacy and safety of the Doxal studied drug in the treatment of chickens 25 daily age with colibacteriose-claspidiosis infection was established. \u0000The diagnosis was made on the basis of anamnestic data, a clinical picture of the disease, the results of pathoanatomical section and bacteriological examination. Microorganisms – pathogens of infection, their level of sensitivity to doxycycline were selected and identified. Therapeutic efficiency of the tested drug was not inferior to the drug-comparison of Doxatib®, production of KRKA DD Novo-Mesto (Slovenia), similar in dosage form and the content of the active substance. \u0000According to the results of laboratory tests, a positive effect of Doxal on the morpho-functional state of the body of chickens in the treatment of acute colibacteriosis-claspidiosis infection was revealed, which is confirmed by normalization with a high percentage of the reliability of morphological and biochemical blood indicators to the values of clinically healthy bird. \u0000Comparative clinical studies have confirmed that Doxal drug is effective chemotherapeutic agent for the treatment of bacterial infections in chickens caused by microorganisms sensitive to doxycycline, and is analogous to Doxatib® by it’s action.","PeriodicalId":21617,"journal":{"name":"Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83250290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"REASONS FOR INCONSISTENCY IN THE QUALITY OF RODENTICIDES (PROCESSED GRAIN, PASTE) WITH BROMАDIALON AND BRODIFACOUM","authors":"R. Ostapiv, S. Humenyuk, L. Samarska, M. Berezyuk","doi":"10.36359/scivp.2022-23-1.16","DOIUrl":"https://doi.org/10.36359/scivp.2022-23-1.16","url":null,"abstract":"Rodenticides are a class of biocides whose primary purpose is to kill rodents. Rodenticides are divided by the mechanism of action into acute and chronic. In Ukraine, most of the registered drugs are of chronic action, as they are more effective against rodents. Bromаdiolone and brodifacoum are the main active substances of rodenticides with a chronic effect. Since these substances absorb well in the ultraviolet spectrum and their concentration in the preparation is low -0.005%, the quality control of chronic rodenticides is carried out, in most cases, by high-performance liquid chromatography. The purpose of the work was to analyze the quantitative content of active substances in solutions of bromаdiolone and brodifacoum and in ready-made rodenticides (grain, paste) during 2012–2022 and to establish possible reasons for non-compliance with the specification. \u0000The study was conducted using a Dionex Ultimate 3000 chromatograph and validated methods for determining the quantitative content of bromаdiolone and brodifacoum in rodenticides. Thirty-six samples of ready-made rodenticides and solutions for their preparation were analyzed. It was found that one third of the examined samples did not meet the requirements of regulatory documentation. Of the 22 ready-made baits for the destruction of rodents, 5 did not meet the specification. Since the active substance in the preparation itself is a little -0.005%, it can be assumed that these results are related to the violation of technological regulations in the production process. However, we found that out of 14 analyzed solutions for the production of ready-made rodenticides, in the form of processed grains or briquettes, 7 do not meet the requirements of the specification. This may be due to errors in the preparation of bromаdiolone and brodifacoum solutions, or to substances that do not correspond to what is stated in the quality certificate. Therefore, it is very important for manufacturing companies to carry out incoming control of raw materials, both substances and solutions for the preparation of ready-made rodenticides.","PeriodicalId":21617,"journal":{"name":"Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology","volume":"73 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79000791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE TENDENCY OF TRANSGENIC PLANTS SPREAD IN 2019-2021","authors":"G. V. Kuchnir, T. R. Levitskyj, G. P. Ryvak, G. Fedor, L. V. Kurylas, T. Y. Senyshyna","doi":"10.36359/scivp.2022-23-1.10","DOIUrl":"https://doi.org/10.36359/scivp.2022-23-1.10","url":null,"abstract":"The achievement of modern biotechnology, in particular genetic engineering in crop production, provides not only the possibility of increasing production, but also more efficient use of natural resources and reduces the negative impact on the environment. Recent publications indicate the widespread distribution of genetically modified (GM) agricultural crops in the world that are currently in commercial use, research and development. All over the world, the most cultivated GM crops are cotton, soybeans, corn, and rapeseed, and the area under these biotechnological crops continues to grow every year. However, the analysis of publicly available sources of information revealed the risks of negative effects of GMOs on human and animal bodies, as well as on the environment and biodiversity. \u0000The article analyzes the results of research on the presence of GM ingredients in plant raw materials, processing products, and feed for productive and non-productive animals for the period 2019-2021. The study was conducted by the polymerase chain reaction method in real time (PCR-RF), according to DSTU ISO 21569:2008 (ISO 21569:2005, IDT), and DSTU ISO 21571:2008 (ISO 21571:2005, IDT). In the studied samples were detected target sequences of the promoter p35S CaMV (cauliflower mosaic virus), promoter p34S FMV, terminator NOS (T-NOS) T1 of the Agrobacterium tumefaciens plasmid, genes Pat, EPSPS, Cry 3A. \u0000Transgenic plants are present in circulation in the agricultural market of Ukraine, and the area under their crops is increasing. \u0000The largest number of GM plants was found in samples of rapeseed, soybeans, and feed for productive and non-productive animals. There was a tendency for an increase in the number of transgenic plants in tested samples. During rapeseed research in 2019, the number of positive samples was 6.5%, in 2020 – 7.4%, and in 2021 – 14.3%. When studying soybeans in 2019, the number of positive samples was 6.7%, in 2020 – 16.7%, and in 2021 – 18.2%. \u0000GM ingredients were also found in feed for productive and non-productive animals. In 2020 16.7% of compound feed for poultry were positive, while in 2021 there were no samples for research. \u0000As for feeds for non-productive animals, during 2019–2020 there were no detected positive samples, while in 2021 the number of positive samples was 12%.","PeriodicalId":21617,"journal":{"name":"Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91164556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DEVELOPMENT OF SPECIFICATION AND CONTROL OF INPUT MATERIALS FOR THE MANUFACTURE OF AMOXICILLINE-TRIHYDRATE BASED PREPARATION","authors":"R. Sachuk, Lidia Kostyshyn, B. Gutyj, Ya. S. Stravsky, T. A. Velesik, O. Katsaraba, U. Tesarivska, S. Zhyhalyuk, L. V. Kurylas, S. A. Ponomareva","doi":"10.36359/scivp.2022-23-1.22","DOIUrl":"https://doi.org/10.36359/scivp.2022-23-1.22","url":null,"abstract":"To characterize and determine the quality of the veterinary medicinal product «Amoxidev 60» (powder for oral use) which contains amoxicillin trihydrate, in addition to physical and chemical tests, it is important to develop a specification and control of input materials intended for its production. \u0000The aim of the study was to create a drug based on amoxicillin trihydrate of appropriate quality and to substantiate the specification of control of input materials intended for its production. \u0000Veterinary drug «Amoxidev» - water-soluble white powder with a specific odor. One gram of the drug contains the active substance: amoxicillin trihydrate – 600 mg and filler: lactose – up to 1 g. \u0000The drug is prescribed to zoo animals - European deer, ornamental birds (pheasant, peacock) for the treatment and prevention of respiratory diseases (rhinotracheitis, bronchopneumonia, pneumonia), digestive (gastroenteritis, enterocolitis), genitourinary systems (metritis, nephritis, rubella) apparatus and soft tissues (injuries, myositis, dermatitis, mastitis) caused by microorganisms sensitive to the drug. \u0000Control of raw materials included the following operations: determination of the mass fraction of amoxicillin trihydrate; testing; preparation of control solution; chromatography; testing of amoxicillin trihydrate for purity; testing of amoxicillin trihydrate for the presence of accompanying impurities; determination of the pH of the test solution and the transparency of the substance solution. \u0000Based on the proposed specification and approaches to laboratory quality control of powder for oral use based on amoxicillin trihydrate developed production documentation that corresponds to the registration dossier for the veterinary drug «Amoxidev 60». The test results are properly investigated and documented in accordance with the Operating Standards Standard (SOP).","PeriodicalId":21617,"journal":{"name":"Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86617046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE VALIDATION OF THE METHOD FOR QUINOLONES RESIDUES DETERMINATION IN SAMPLES OF MILK, EGG POWDER, TISSUES, BLOOD PLASMA OF ANIMALS USING THE METHOD OF ENZYME-LINKED IMMUNOSORBENT ASSAY","authors":"D. Yanovych, Z. Zasadna, M. Rydchuk, S. Plotytsia, S. Kislova, O. Pazderska","doi":"10.36359/scivp.2022-23-1.29","DOIUrl":"https://doi.org/10.36359/scivp.2022-23-1.29","url":null,"abstract":"The article presents the results of validation of screening method for the determination of quinolone antibiotics residues (enrofloxacin, ciprofloxacin, marbofloxacin, danofloxacin) in tissue samples, egg powder, milk and blood plasma using RIDASCREEN®Chinolone/Quinolones test kits from R-Biopharm (Germany). According to the information provided by the manufacturer of the kit, the quantitative determination of quinolones content of can be carried out in the following matrices: shrimp, fish, eggs, meat. In accordance with European Commission Decision 2002/657/EC, the methods proposed by the manufacturer were validated taking into account the maximum permissible levels (MRLs), and the results of the development of sample preparation techniques for the assay of liver, milk and blood plasma for various representatives of antibiotics of quinolone group and the corresponding validation results were presented. \u0000The suitability of the technique was confirmed on the basis of establishing of main validation parameters for screening methods (technical threshold and cut-off factor) using control (blank) samples (muscle and parenchymal tissues of poultry and animals, cow’s milk, blood plasma of broiler chickens and egg powder) and spiked with quinolones standard solutions at the level of ½ MRL according to the \"added-found\" criterion. The main advantages of the developed method are the simplicity of performance, rapidity and economic efficiency. \u0000The reliability of the results obtained by the developed method was verified using confirmatory UPLC-MS/MS method. The results of comparative study of control (blank) samples of chicken muscle tissues, egg powder and cow’s milk and spiked with enrofloxacin standard solution at the level of 2.5 and 5.0 μg/kg are presented. \u0000The proposed methods for liver, milk and blood plasma sample preparation can be used to study withdrawal periods and bioequivalence of veterinary drugs based on quinolone antibiotics.","PeriodicalId":21617,"journal":{"name":"Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78324908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}