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Obtuse-angled separation of chiral resonances with planar asymmetry–induced tunability of quality factors 具有平面不对称诱导品质因子可调性的手性共振钝角分离
IF 11.7 1区 综合性期刊
Science Advances Pub Date : 2025-07-23 DOI: 10.1126/sciadv.adu4875
Minsu Jeong, Jihae Lee, Seokwoo Kim, Xiangxin Gong, Rouli Fang, Yuhui Yang, Sang Hoon Chae, Junsuk Rho
{"title":"Obtuse-angled separation of chiral resonances with planar asymmetry–induced tunability of quality factors","authors":"Minsu Jeong,&nbsp;Jihae Lee,&nbsp;Seokwoo Kim,&nbsp;Xiangxin Gong,&nbsp;Rouli Fang,&nbsp;Yuhui Yang,&nbsp;Sang Hoon Chae,&nbsp;Junsuk Rho","doi":"10.1126/sciadv.adu4875","DOIUrl":"10.1126/sciadv.adu4875","url":null,"abstract":"<div >Photonic crystal (PhC) supports Bloch resonances that confine electromagnetic energy within the subwavelength thickness and enable polarization modulation through their intrinsic mode states. If a PhC generates chiral resonances, then it can selectively enhance or suppress specific circular polarizations, making it ideal for chiral optics. Here, we devise a design strategy to realize chiral resonant modes with large angular divergence and tunable quality factors (<i>Q</i> factors) by introducing planar structural perturbations. The <i>Q</i> factor exhibits an inverse-square dependence on perturbation strength, consistent with the behavior of quasi-bound states in the continuum. Theoretical and experimental results demonstrate chiral modes with high circular dichroism, large separation angles, and high-<i>Q</i> factors. We further couple 2D excitons to these resonant modes, achieving spatially separated chiral emission. Using Brillouin zone folding, we translate bound modes at high-symmetry points into the radiative region, suggesting a strategy to control polarization, group velocity, and topology in photonic systems.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 30","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adu4875","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144684969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dynamic change of calcium-rich compartments during coccolithophore biomineralization 球石藻生物矿化过程中富钙区室的动态变化
IF 11.7 1区 综合性期刊
Science Advances Pub Date : 2025-07-23 DOI: 10.1126/sciadv.adv0618
Alexander Triccas, Daniel M. Chevrier, Mariana Verezhak, Johannes Ihli, Manuel Guizar-Sicairos, Mirko Holler, André Scheffel, Noriaki Ozaki, Virginie Chamard, Rachel Wood, Tilman A. Grünewald, Fabio Nudelman
{"title":"Dynamic change of calcium-rich compartments during coccolithophore biomineralization","authors":"Alexander Triccas,&nbsp;Daniel M. Chevrier,&nbsp;Mariana Verezhak,&nbsp;Johannes Ihli,&nbsp;Manuel Guizar-Sicairos,&nbsp;Mirko Holler,&nbsp;André Scheffel,&nbsp;Noriaki Ozaki,&nbsp;Virginie Chamard,&nbsp;Rachel Wood,&nbsp;Tilman A. Grünewald,&nbsp;Fabio Nudelman","doi":"10.1126/sciadv.adv0618","DOIUrl":"10.1126/sciadv.adv0618","url":null,"abstract":"<div >Coccolithophores are abundant marine phytoplankton that produce biomineralized calcite scales, called coccoliths, which sequester substantial amounts of carbon and play a substantial role in biogeochemical cycles. However, mechanisms underlying the storage and transport of ions essential for calcification remain unresolved. We used ptychographic x-ray computed tomography under cryogenic conditions to visualize intracellular calcium-rich structures involved in the storage of calcium ions in the coccolithophore species <i>Chrysotila carterae</i>. During calcification, we observed a range of structures, from small electron-dense bodies within larger compartments to denser and distributed globular compartments, before returning to small bodies once scale formation is complete. Nanobeam-scanning x-ray fluorescence measurements further revealed that these electron-dense bodies are rich in phosphorus and calcium (molar ratio of ~4:1). The dynamic nature of structures suggests that these bodies are part of the required cellular calcium ion transport pathways, a fundamental process critical for understanding the response of coccolithophores to climate change.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 30","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adv0618","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144685069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
M6A-dependent RNA condensation underlies FUS autoregulation and can be harnessed for ALS therapy development m6a依赖的RNA缩合是FUS自动调节的基础,可以用于ALS治疗的发展
IF 11.7 1区 综合性期刊
Science Advances Pub Date : 2025-07-23 DOI: 10.1126/sciadv.adx1357
Wan-Ping Huang, Vedanth Kumar, Karen Yap, Haiyan An, Sabin J. John, Rachel E. Hodgson, Anna Sanchez Avila, Emily Day, Brittany C. S. Ellis, Tek Hong Chung, Jenny Lord, Michaela Müller-McNicoll, Eugene V. Makeyev, Tatyana A. Shelkovnikova
{"title":"M6A-dependent RNA condensation underlies FUS autoregulation and can be harnessed for ALS therapy development","authors":"Wan-Ping Huang,&nbsp;Vedanth Kumar,&nbsp;Karen Yap,&nbsp;Haiyan An,&nbsp;Sabin J. John,&nbsp;Rachel E. Hodgson,&nbsp;Anna Sanchez Avila,&nbsp;Emily Day,&nbsp;Brittany C. S. Ellis,&nbsp;Tek Hong Chung,&nbsp;Jenny Lord,&nbsp;Michaela Müller-McNicoll,&nbsp;Eugene V. Makeyev,&nbsp;Tatyana A. Shelkovnikova","doi":"10.1126/sciadv.adx1357","DOIUrl":"10.1126/sciadv.adx1357","url":null,"abstract":"<div >Mutations in the <i>FUS</i> gene cause aggressive amyotrophic lateral sclerosis (ALS-FUS). Beyond mRNA, <i>FUS</i> generates partially processed transcripts retaining introns 6 and 7. We demonstrate that these FUSint6&amp;7-RNA molecules form nuclear condensates, scaffolded by the highly structured intron 7 and associated with nuclear speckles. Using hybridization-proximity labeling proteomics, we show that the FUSint6&amp;7-RNA condensates are enriched for splicing factors and the N6-methyladenosine (m6A) reader YTHDC1. These ribonucleoprotein structures facilitate posttranscriptional FUS splicing and depend on m6A/YTHDC1 for integrity. In cells expressing mutant FUS, FUSint6&amp;7-RNAs become hypermethylated, which in turn stimulates their condensation and splicing. We further show that FUS protein is repelled by m6A. Thus, ALS-FUS mutations may cause abnormal activation of FUS posttranscriptional splicing through altered RNA methylation. Notably, ectopic expression of FUS intron 7 sequences dissolves endogenous FUSint6&amp;7-RNA condensates, down-regulating FUS mRNA and protein. Our findings reveal a condensation-dependent mechanism regulating FUS splicing, with possible therapeutic implications for ALS.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 30","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adx1357","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144685071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Targeting tumor-associated CCR2+ macrophages to inhibit pancreatic cancer recurrence following irreversible electroporation 靶向肿瘤相关CCR2 +巨噬细胞抑制不可逆电穿孔后胰腺癌复发
IF 11.7 1区 综合性期刊
Science Advances Pub Date : 2025-07-23 DOI: 10.1126/sciadv.adw2937
Weichen Xu, Shaoyue Li, Xuexia Shan, Qiao Wang, Xinhua Chen, Shengbo Wu, Yincheng Gao, Dandan Shan, Shisi Ding, Weiwei Ren, Xiaodong Hou, Shuo Liu, Taixia Wang, Yuting Shen, Zhiyuan Niu, Huixiong Xu, Liping Sun, Wenwen Yue
{"title":"Targeting tumor-associated CCR2+ macrophages to inhibit pancreatic cancer recurrence following irreversible electroporation","authors":"Weichen Xu,&nbsp;Shaoyue Li,&nbsp;Xuexia Shan,&nbsp;Qiao Wang,&nbsp;Xinhua Chen,&nbsp;Shengbo Wu,&nbsp;Yincheng Gao,&nbsp;Dandan Shan,&nbsp;Shisi Ding,&nbsp;Weiwei Ren,&nbsp;Xiaodong Hou,&nbsp;Shuo Liu,&nbsp;Taixia Wang,&nbsp;Yuting Shen,&nbsp;Zhiyuan Niu,&nbsp;Huixiong Xu,&nbsp;Liping Sun,&nbsp;Wenwen Yue","doi":"10.1126/sciadv.adw2937","DOIUrl":"10.1126/sciadv.adw2937","url":null,"abstract":"<div >Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal malignancy with pronounced resistance to conventional therapies. Irreversible electroporation (IRE) is a promising therapy for PDAC; however, its clinical efficacy is limited by a high recurrence rate. Here, using a preclinical PDAC model, we characterized the tumor immune microenvironment following insufficient IRE (iIRE) through single-cell RNA sequencing. We found that iIRE induces a CCR2<sup>+</sup> tumor-associated macrophage (CCR2<sup>+</sup> TAM)–mediated immunosuppressive microenvironment in residual tumors. Consequently, we developed a macrophage-based proteolipid vesicle (mPLV) coencapsulating the CCR2 antagonist PF-4136309 (PF) and gemcitabine (GEM), named PF/GEM@mPLV. Our findings suggest that PF/GEM@mPLV achieves high drug accumulation within tumors through iIRE-induced inflammation. Reduction of CCR2<sup>+</sup> TAMs enhances antitumor immunity and improves chemotherapeutic response. PF/GEM@mPLV markedly inhibits tumor recurrence following iIRE, diminishes hepatic metastases, and prolongs survival in preclinical PDAC models. These findings uncover the role of CCR2<sup>+</sup> TAMs in iIRE-induced immunosuppression, offering a promising strategy to enhance the clinical potential of IRE in PDAC.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 30","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adw2937","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144684899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A nociceptive amygdala-striatal pathway modulating affective-motivational pain 伤害性杏仁核-纹状体通路调节情感性-动机性疼痛
IF 11.7 1区 综合性期刊
Science Advances Pub Date : 2025-07-23 DOI: 10.1126/sciadv.ado2837
Jessica A. Wojick, Alekh Paranjapye, Juliann K. Chiu, Corinna S. Oswell, Malaika Mahmood, Lisa M. Wooldridge, Blake A. Kimmey, Raquel Adaia Sandoval Ortega, Nora M. McCall, Seungmin Han, Jacqueline W. K. Wu, Maxx Yung, Lindsay L. Ejoh, Samar Nasser Chehimi, Richard C. Crist, Benjamin C. Reiner, Erica Korb, Gregory Corder
{"title":"A nociceptive amygdala-striatal pathway modulating affective-motivational pain","authors":"Jessica A. Wojick,&nbsp;Alekh Paranjapye,&nbsp;Juliann K. Chiu,&nbsp;Corinna S. Oswell,&nbsp;Malaika Mahmood,&nbsp;Lisa M. Wooldridge,&nbsp;Blake A. Kimmey,&nbsp;Raquel Adaia Sandoval Ortega,&nbsp;Nora M. McCall,&nbsp;Seungmin Han,&nbsp;Jacqueline W. K. Wu,&nbsp;Maxx Yung,&nbsp;Lindsay L. Ejoh,&nbsp;Samar Nasser Chehimi,&nbsp;Richard C. Crist,&nbsp;Benjamin C. Reiner,&nbsp;Erica Korb,&nbsp;Gregory Corder","doi":"10.1126/sciadv.ado2837","DOIUrl":"10.1126/sciadv.ado2837","url":null,"abstract":"<div >The basolateral amygdala (BLA) assigns valence to sensory stimuli, with a dedicated nociceptive ensemble encoding the negative valence of pain. However, the effects of chronic pain on the transcriptomic signatures and projection architecture of this BLA nociceptive ensemble are not well understood. Here, we show that optogenetic inhibition of the nociceptive BLA ensemble reduces affective-motivational behaviors in chronic neuropathic pain. Single-nucleus RNA sequencing revealed peripheral injury–induced changes in genetic pathways involved in axonal and presynaptic organization in nociceptive BLA neurons. Next, we identified a previously uncharacterized nociceptive hotspot in the nucleus accumbens shell that is innervated by BLA nociceptive neurons. Axonal calcium imaging of BLA projections to the accumbens and chemogenetic inhibition of this pathway revealed pain-related transmission from the amygdala to the medial nucleus accumbens, facilitating both acute and chronic pain affective-motivational behaviors. Together, this work defines a critical nociceptive amygdala-striatal circuit underlying pain unpleasantness across pain states.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 30","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.ado2837","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144684971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A superstrong, decarbonizing structural material enabled by microbe-assisted cell wall engineering via a biomechanochemical process 一种超强的脱碳结构材料,通过生物力学过程通过微生物辅助细胞壁工程实现
IF 11.7 1区 综合性期刊
Science Advances Pub Date : 2025-07-23 DOI: 10.1126/sciadv.ady0183
Ziyang Lu, Luhe Qi, Junqing Chen, Cai Lu, Jing Huang, Lu Chen, Yuying Wu, Jiahao Feng, Jinyou Lin, Ze Liu, Erlantz Lizundia, Chaoji Chen
{"title":"A superstrong, decarbonizing structural material enabled by microbe-assisted cell wall engineering via a biomechanochemical process","authors":"Ziyang Lu,&nbsp;Luhe Qi,&nbsp;Junqing Chen,&nbsp;Cai Lu,&nbsp;Jing Huang,&nbsp;Lu Chen,&nbsp;Yuying Wu,&nbsp;Jiahao Feng,&nbsp;Jinyou Lin,&nbsp;Ze Liu,&nbsp;Erlantz Lizundia,&nbsp;Chaoji Chen","doi":"10.1126/sciadv.ady0183","DOIUrl":"10.1126/sciadv.ady0183","url":null,"abstract":"<div >Lightweight and high-strength structural materials promise exceptional applications in advanced engineering fields. As a productive and sustainable material, wood exhibits exceptional potential to be converted into high-performance structural materials. Inspired by ancient buried wood—a naturally formed material after wood endures in microbial-rich and high-pressure environments for thousands of years—here, we demonstrate a biomechanochemical process to rapidly transform natural wood into artificial ancient buried wood (named Bio-Strong-Wood). Biotreatment depolymerizes the lignin and softens the cell wall. Then, Bio-Strong-Wood components are linked via a strong network of hydrogen and covalent bonds through the mechanochemical treatment. This results in a substantially enhanced mechanical strength (539 ± 21.7 megapascals), which outperforms the SAE 304 stainless steel. In addition, life cycle and technoeconomic assessments reveal that the obtained material achieves negative carbon emissions of 1.17 kilograms of carbon dioxide equivalent per kilogram. Overall, our work provides an economically competitive, environmentally sustainable, and decarbonizing alternative to existing structural materials.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 30","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.ady0183","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144684947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Engineering hybrid nanoparticles for targeted codelivery of triptolide and CYP3A4-siRNA against pulmonary metastatic melanoma 工程混合纳米颗粒靶向共递送雷公藤甲素和CYP3A4 -siRNA治疗肺转移性黑色素瘤
IF 11.7 1区 综合性期刊
Science Advances Pub Date : 2025-07-23 DOI: 10.1126/sciadv.adv6990
Yongwei Gu, Aixue Li, Yuanye Zeng, Mengyuan He, Fu Qi, Rongmei Liu, Huanhuan Cai, Dan Li, Xiaomeng Tang, Zhiqin Fu, Xin Wu, Jiyong Liu
{"title":"Engineering hybrid nanoparticles for targeted codelivery of triptolide and CYP3A4-siRNA against pulmonary metastatic melanoma","authors":"Yongwei Gu,&nbsp;Aixue Li,&nbsp;Yuanye Zeng,&nbsp;Mengyuan He,&nbsp;Fu Qi,&nbsp;Rongmei Liu,&nbsp;Huanhuan Cai,&nbsp;Dan Li,&nbsp;Xiaomeng Tang,&nbsp;Zhiqin Fu,&nbsp;Xin Wu,&nbsp;Jiyong Liu","doi":"10.1126/sciadv.adv6990","DOIUrl":"10.1126/sciadv.adv6990","url":null,"abstract":"<div >Pulmonary metastatic melanoma (PMM) is an aggressive malignancy with limited response and rapid resistance to clinical chemotherapy, radiotherapy, immunotherapy, and biological therapies. Here, we developed a targeted biomimetic drug delivery system, TP-siRC@tHyNPs, by fusing exosomes derived from engineered cells overexpressing DR5 single-chain variable fragments (DR5-Exo) with liposomes coencapsulating triptolide (TP) and CYP3A4-siRNA (TP-siRC@Lip). DR5-Exo facilitated the targeted delivery of drug to tumor cells through DR5 receptor recognition and simultaneously activated apoptotic pathways. Moreover, CYP3A4-siRNA effectively prolonged the half-life of TP, thereby enhancing its antiproliferative and pro-apoptotic effects. Mechanistic studies revealed that TP-siRC@tHyNPs induced immunogenic cell death, reprogrammed macrophage polarization, arrested cell cycle progression, and triggered apoptotic pathways. In vivo experiments demonstrated that TP-siRC@tHyNPs specifically accumulated in lung tissue, notably inhibiting the growth of PMM while exhibiting negligible toxicity in tumor-bearing mice. Overall, this study provides a promising strategy for targeting PMM treatment, improving therapeutic efficacy while reducing off-target toxicity.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 30","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adv6990","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144684990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Photoacoustic and fluorescence hybrid microscope for cortex-wide imaging of neurovascular dynamics with subcellular resolution 光声和荧光混合显微镜用于神经血管动力学的亚细胞分辨率全皮层成像
IF 11.7 1区 综合性期刊
Science Advances Pub Date : 2025-07-23 DOI: 10.1126/sciadv.adw5275
Liangjian Liu, Zhiqiang Xu, Zhenjie Lai, Bin Xu, Taofeng Wu, Guangying Ma, Hongdong Zhang, Jiahao Li, Weilei Ma, Tiancheng Lei, Xiufeng Li, Zeyi Guo, Zheng Song, Ningbo Chen, Shiwei Ye, Jing Meng, Puxiang Lai, Feng Shen, Junlei Chang, Yingjie Zhu, Hairong Zheng, Wei Zheng, Chengbo Liu
{"title":"Photoacoustic and fluorescence hybrid microscope for cortex-wide imaging of neurovascular dynamics with subcellular resolution","authors":"Liangjian Liu,&nbsp;Zhiqiang Xu,&nbsp;Zhenjie Lai,&nbsp;Bin Xu,&nbsp;Taofeng Wu,&nbsp;Guangying Ma,&nbsp;Hongdong Zhang,&nbsp;Jiahao Li,&nbsp;Weilei Ma,&nbsp;Tiancheng Lei,&nbsp;Xiufeng Li,&nbsp;Zeyi Guo,&nbsp;Zheng Song,&nbsp;Ningbo Chen,&nbsp;Shiwei Ye,&nbsp;Jing Meng,&nbsp;Puxiang Lai,&nbsp;Feng Shen,&nbsp;Junlei Chang,&nbsp;Yingjie Zhu,&nbsp;Hairong Zheng,&nbsp;Wei Zheng,&nbsp;Chengbo Liu","doi":"10.1126/sciadv.adw5275","DOIUrl":"10.1126/sciadv.adw5275","url":null,"abstract":"<div >Simultaneously monitoring cortex-wide microvascular dynamics, blood oxygenation metabolism, and neuronal activities with high spatiotemporal resolution is essential for understanding neurovascular coupling (NVC). However, it remains a challenge for existing neuroimaging tools. Here, we report a linear transducer array–based hybrid microscope (LiTA-HM) that integrates photoacoustic microscopy and confocal fluorescence microscopy to achieve simultaneous neurovascular imaging with a field of view (FOV) of 6 millimeters by 5 millimeters, a spatial resolution of ~6 micrometers, and a temporal resolution of 0.8 seconds. We design an array of eight miniature transducers to enlarge FOV and optimize a polygon mirror–based ultrafast scanner to achieve real-time imaging. Proof-of-concept imaging experiments were conducted on awake mice. Different couplings between thousands of neuron somas and vessel branches down to capillaries were observed in hypoxia and anesthesia experiments. Spatiotemporal correlations and functional connectivity in NVC were uncovered for spreading depolarization waves occurred in a drug-induced epilepsy experiment. We expect that LiTA-HM will enable broad applications of neuroimaging.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 30","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adw5275","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144685066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In-body current path manipulation with minimal attenuation 最小衰减的体内电流路径操纵
IF 11.7 1区 综合性期刊
Science Advances Pub Date : 2025-07-23 DOI: 10.1126/sciadv.adx5922
Donghyeon Kang, Byung-Joon Park, Joon-Ha Hwang, Young-Jun Kim, So-Hee Kim, Hyun Woo Kim, Ki Jun Yu, Jinyoung Jeon, Hyeon Yeong Lee, Youngwook Chung, Soo Hyun Nam, Byung-Ok Choi, Sang-Woo Kim
{"title":"In-body current path manipulation with minimal attenuation","authors":"Donghyeon Kang,&nbsp;Byung-Joon Park,&nbsp;Joon-Ha Hwang,&nbsp;Young-Jun Kim,&nbsp;So-Hee Kim,&nbsp;Hyun Woo Kim,&nbsp;Ki Jun Yu,&nbsp;Jinyoung Jeon,&nbsp;Hyeon Yeong Lee,&nbsp;Youngwook Chung,&nbsp;Soo Hyun Nam,&nbsp;Byung-Ok Choi,&nbsp;Sang-Woo Kim","doi":"10.1126/sciadv.adx5922","DOIUrl":"10.1126/sciadv.adx5922","url":null,"abstract":"<div >Wireless power transfer–based neuromodulation has emerged as a promising alternative to battery-powered implants. However, its practical application is hindered by limited therapeutic efficacy resulting from low power transfer efficiency, shallow penetration depth, and safety concerns. In this study, we report an in-body current path manipulation and concentration for advanced targeted neuromodulation, overcoming the limitations of conventional technologies. By implanting a focusing electrode, we were able to direct the triboelectric current, which has a low frequency and high impedance generated by human movement, toward the target area, with the concentrated current exhibiting minimal attenuation regardless of the electrode size, implantation site, and depth. Applying our technology to modulate damaged neural systems confirmed therapeutic efficacy and validated safety, demonstrating its potential for next-generation targeted neuromodulation.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 30","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adx5922","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144684901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Neuropeptide Y neurons surrounding the locus coeruleus inhibit noradrenergic system activity to reduce anxiety 蓝斑周围的神经肽Y神经元抑制去甲肾上腺素能系统的活性以减少焦虑
IF 11.7 1区 综合性期刊
Science Advances Pub Date : 2025-07-23 DOI: 10.1126/sciadv.adq0011
Danai Riga, Karlijn L. Kooij, Kelly Rademakers, Inge G. Wolterink-Donselaar, Onur Basak, Frank J. Meye
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