Science Advances最新文献

Topology-imprinting in nonlinear metasurfaces 非线性超表面的拓扑印迹

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-06-13 DOI: 10.1126/sciadv.adv5190

Jiannan Gao, Hooman Barati Sedeh, Dmitrii Tsvetkov, Danilo Gomes Pires, Maria Antonietta Vincenti, Yun Xu, Ivan Kravchenko, Renee George, Michael Scalora, Liang Feng, Natalia M. Litchinitser

{"title":"Topology-imprinting in nonlinear metasurfaces","authors":"Jiannan Gao, Hooman Barati Sedeh, Dmitrii Tsvetkov, Danilo Gomes Pires, Maria Antonietta Vincenti, Yun Xu, Ivan Kravchenko, Renee George, Michael Scalora, Liang Feng, Natalia M. Litchinitser","doi":"10.1126/sciadv.adv5190","DOIUrl":"10.1126/sciadv.adv5190","url":null,"abstract":"<div >Flat optical components, or metasurfaces, have transformed optical imaging, data storage, information processing, and biomedical applications by providing unprecedented control over light-matter interactions. These nano-engineered structures enable compact, multidimensional manipulation of light’s amplitude, phase, polarization, and wavefront, producing scalar and vector beams with unique properties such as orbital angular momentum and knotted topologies. This flexibility has potential applications in optical communication and imaging, particularly in complex environments such as atmospheric turbulence and undersea scattering. However, designing metasurfaces for shorter wavelengths, such as visible and ultraviolet light, remains challenging due to fabrication limitations and material absorption. Here, we introduce an innovative concept called topology imprinting using judiciously designed all-dielectric nonlinear optical metasurfaces to replicate desired waveforms at fundamental and harmonic frequencies, opening promising avenues for advanced photonic applications.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 24","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adv5190","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144278979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Armored human CAR Treg cells with PD1 promoter-driven IL-10 have enhanced suppressive function 具有PD1启动子驱动IL-10的装甲人CAR - T细胞具有增强的抑制功能

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-06-13 DOI: 10.1126/sciadv.adx7845

Dominic A. Boardman, Sonya Mangat, Jana K. Gillies, Lorna Leon, Vivian C. W. Fung, Manjurul Haque, Majid Mojibian, Torin Halvorson, Qing Huang, Karoliina Tuomela, Christine M. Wardell, Andrew Brown, Avery J. Lam, Megan K. Levings

{"title":"Armored human CAR Treg cells with PD1 promoter-driven IL-10 have enhanced suppressive function","authors":"Dominic A. Boardman, Sonya Mangat, Jana K. Gillies, Lorna Leon, Vivian C. W. Fung, Manjurul Haque, Majid Mojibian, Torin Halvorson, Qing Huang, Karoliina Tuomela, Christine M. Wardell, Andrew Brown, Avery J. Lam, Megan K. Levings","doi":"10.1126/sciadv.adx7845","DOIUrl":"10.1126/sciadv.adx7845","url":null,"abstract":"<div >Regulatory T cell (T<sub>reg</sub> cell) therapy has been transformed through the use of chimeric antigen receptors (CARs). We previously found that human T<sub>reg</sub> cells minimally produce IL-10 and have a limited capacity to control innate immunity compared to type 1 regulatory T cells (T<sub>r</sub>1 cells). To create “hybrid” CAR T<sub>reg</sub> cells with T<sub>r</sub>1 cell-like properties, we examined whether the <i>PDCD1</i> locus could be exploited to endow T<sub>reg</sub> cells with CAR-regulated IL-10 expression. CRISPR-mediated PD1 deletion increased CAR T<sub>reg</sub> cell activation, and knock-in of <i>IL10</i> under control of the PD1 promoter resulted in CAR-induced IL-10 secretion. <i>IL10</i> knock-in improved CAR T<sub>reg</sub> cell function, as determined by increased suppression of dendritic cells and alloantigen- and islet autoantigen–specific T cells. In vivo, <i>IL10</i> knock-in CAR T<sub>reg</sub> cells were stable, safe, and suppressed dendritic cells and xenogeneic graft-versus-host disease. CRISPR-mediated engineering to simultaneously remove an inhibitory signal and enhance a suppressive mechanism is a previously unexplored approach to improve CAR T<sub>reg</sub> cell potency.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 24","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adx7845","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144278974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-Majorana origin of anomalous current-phase relation and Josephson diode effect in Bi2SE3/NbSe2 Josephson junctions Bi 2 SE 3 /NbSe 2 Josephson结中异常电流-相位关系的非majorana起源和Josephson二极管效应

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-06-13 DOI: 10.1126/sciadv.adw6925

Andrei Kudriashov, Xiangyu Zhou, Razmik A. Hovhannisyan, Alexander S. Frolov, Leonid Elesin, Yi Bo Wang, Ekaterina V. Zharkova, Takashi Taniguchi, Kenji Watanabe, Zheng Liu, Kostya S. Novoselov, Lada V. Yashina, Xin Zhou, Denis A. Bandurin

{"title":"Non-Majorana origin of anomalous current-phase relation and Josephson diode effect in Bi2SE3/NbSe2 Josephson junctions","authors":"Andrei Kudriashov, Xiangyu Zhou, Razmik A. Hovhannisyan, Alexander S. Frolov, Leonid Elesin, Yi Bo Wang, Ekaterina V. Zharkova, Takashi Taniguchi, Kenji Watanabe, Zheng Liu, Kostya S. Novoselov, Lada V. Yashina, Xin Zhou, Denis A. Bandurin","doi":"10.1126/sciadv.adw6925","DOIUrl":"10.1126/sciadv.adw6925","url":null,"abstract":"<div >Josephson junctions (JJs) are key to superconducting quantum technologies and the search for self-conjugate quasiparticles potentially useful for fault-tolerant quantum computing. In topological insulator (TI)–based JJs, measuring the current-phase relation (CPR) can reveal unconventional effects such as Majorana bound states (MBS) and nonreciprocal transport. However, reconstructing CPR as a function of magnetic field has not been attempted. Here, we present a platform for field-dependent CPR measurements in planar JJs made of NbSe<sub>2</sub> and few-layer Bi<sub>2</sub>Se<sub>3</sub>. When a flux quantum <span><math><mrow><msub><mi>Φ</mi><mn>0</mn></msub></mrow></math></span> threads the junction, we observe anomalous peak-dip CPR structure and nonreciprocal supercurrent flow. We show that these arise from a nonuniform supercurrent distribution that also leads to a robust and tunable Josephson diode effect. Furthermore, despite numerous previous studies, we find no evidence of MBS. Our results establish magnetic field–dependent CPR as a powerful probe of TI-based superconducting devices and offer design strategies for nonreciprocal superconducting electronics.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 24","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adw6925","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144278982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Asymmetric alkyl-alkyl coupling between electron-deficient and unactivated alkenes to access α-chiral phosphines by Ni catalysis 缺电子烯烃与未活化烯烃之间的不对称烷基-烷基偶联以Ni催化获得α-手性膦

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-06-13 DOI: 10.1126/sciadv.adv6571

Jian-Yu Zou, Fang-Li Xing, Quan-Xing Zi, Hai-Wu Du, Meiwan Chen, Wei Shu

{"title":"Asymmetric alkyl-alkyl coupling between electron-deficient and unactivated alkenes to access α-chiral phosphines by Ni catalysis","authors":"Jian-Yu Zou, Fang-Li Xing, Quan-Xing Zi, Hai-Wu Du, Meiwan Chen, Wei Shu","doi":"10.1126/sciadv.adv6571","DOIUrl":"10.1126/sciadv.adv6571","url":null,"abstract":"<div >α-Chiral phosphorus compounds have broad applications as organic catalysts or ligands in organic chemistry and related areas. Herein, we disclose a Ni-catalyzed enantioselective cross-hydrodimerization of alkenyl phosphine sulfides with unactivated alkenes to access α-chiral phosphine sulfides, representing the first example of asymmetric hydrodimerization of electron-deficient alkenes with electron-rich alkenes. Key to success is the precise recognition between electron-deficient alkenes and electron-rich alkenes and streamlined alkyl-alkyl bond forming with the control of chemo-, regio-, and enantioselectivity. This strategy requires alkenes as sole precursors for asymmetric alkyl-alkyl cross-coupling, circumventing the use of stoichiometric amounts of alkyl electrophiles or alkyl nucleophiles as coupling partners. The mild conditions tolerate a wide range of functional groups, providing direct access to α-chiral phosphines through a carbon-carbon bond-forming process without prefunctionalized coupling precursors.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 24","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adv6571","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144278984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered chirality of one-dimensional nanowires 一维纳米线的工程手性

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-06-13 DOI: 10.1126/sciadv.adx4761

Megan Briggeman, Elliott Mansfield, Johannes Kombe, François Damanet, Hyungwoo Lee, Yuhe Tang, Muqing Yu, Sayanwita Biswas, Jianan Li, Mengchen Huang, Chang-Beom Eom, Patrick Irvin, Andrew J. Daley, Jeremy Levy

{"title":"Engineered chirality of one-dimensional nanowires","authors":"Megan Briggeman, Elliott Mansfield, Johannes Kombe, François Damanet, Hyungwoo Lee, Yuhe Tang, Muqing Yu, Sayanwita Biswas, Jianan Li, Mengchen Huang, Chang-Beom Eom, Patrick Irvin, Andrew J. Daley, Jeremy Levy","doi":"10.1126/sciadv.adx4761","DOIUrl":"10.1126/sciadv.adx4761","url":null,"abstract":"<div >The origin and function of chirality in DNA, proteins, and other building blocks of life represent a central question in biology. Observations of spin polarization and magnetization associated with electron transport through chiral molecules, known collectively as the chiral induced spin selectivity effect, suggest that chirality improves electron transfer. Using reconfigurable nanoscale control over conductivity at the LaAlO<sub>3</sub>/SrTiO<sub>3</sub> interface, we create chiral electron potentials that explicitly lack mirror symmetry. Quantum transport measurements on these chiral nanowires reveal enhanced electron pairing persisting to high magnetic fields (up to 18 tesla) and oscillatory transmission resonances as functions of both magnetic field and chemical potential. We interpret these resonances as arising from an engineered axial spin-orbit interaction within the chiral region. The ability to create one-dimensional electron waveguides with this specificity creates opportunities to test, via analog quantum simulation, theories about chirality and spin-polarized electron transport in one-dimensional geometries.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 24","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adx4761","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trapping and imaging dynamic battery nanointerfaces via electrified cryo-EM 电气化低温电镜捕获和成像动态电池纳米界面

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-06-13 DOI: 10.1126/sciadv.adv3191

Chongzhen Wang, Jung Tae Kim, Xintong Yuan, Jin Koo Kim, Bo Liu, Min-ho Kim, Dingyi Zhao, Matthew Mecklenburg, Yuzhang Li

{"title":"Trapping and imaging dynamic battery nanointerfaces via electrified cryo-EM","authors":"Chongzhen Wang, Jung Tae Kim, Xintong Yuan, Jin Koo Kim, Bo Liu, Min-ho Kim, Dingyi Zhao, Matthew Mecklenburg, Yuzhang Li","doi":"10.1126/sciadv.adv3191","DOIUrl":"10.1126/sciadv.adv3191","url":null,"abstract":"<div >The electrified interface between a liquid and a solid underpins diverse phenomena, from ion-transfer during battery operation to action potentials enabling biological communication. However, conventional tools are blind to the nanoscale dynamics of this metastable interface. Here, we leverage electrified cryo–electron microscopy (eCryo-EM), a technique that rapidly freezes and kinetically traps these dynamic, nonequilibrium states during battery operation for nanoscale characterization. Collective snapshots of the electrified interface at controlled time intervals quantifies early-stage growth kinetics of the solid electrolyte interphase (SEI), a passivation film that governs electron and ion transport. Unexpectedly, the diffusivity of charged species of the two SEI films with differing chemistry and performance are estimated to be within 10% of the other, indicated by the slope of their diffusion-limited SEI growth regimes. Instead, the slope of the reaction-limited SEI growth regimes differs by a factor of 3, suggesting that lowered reactivity of the high-performance electrolyte is largely responsible for its high coulombic efficiency.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 24","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adv3191","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144278868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chordin-mediated BMP shuttling patterns the secondary body axis in a cnidarian 脊索介导的BMP穿梭模式在针胞体的次级体轴

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-06-13 DOI: 10.1126/sciadv.adu6347

David Mörsdorf, Maria Mandela Prünster, Paul Knabl, Grigory Genikhovich

{"title":"Chordin-mediated BMP shuttling patterns the secondary body axis in a cnidarian","authors":"David Mörsdorf, Maria Mandela Prünster, Paul Knabl, Grigory Genikhovich","doi":"10.1126/sciadv.adu6347","DOIUrl":"10.1126/sciadv.adu6347","url":null,"abstract":"<div >Bone morphogenetic protein (BMP) signaling patterns secondary body axes throughout Bilateria and in the bilaterally symmetric corals and sea anemones. Chordin-mediated “shuttling” of BMP ligands is responsible for the BMP signaling gradient formation in many bilaterians and, possibly, also in the sea anemone <i>Nematostella</i>, making BMP shuttling a candidate ancestral mechanism for generating bilaterality. However, <i>Nematostella</i> Chordin might be a local inhibitor of BMP rather than a shuttle. To choose between these options, we tested whether extracellular mobility of Chordin, a hallmark of shuttling but dispensable for local inhibition, is required for patterning in <i>Nematostella</i>. By generating localized Chordin sources in the Chordin morphant background, we showed that mobile Chordin is necessary and sufficient to establish a peak of BMP signaling opposite to Chordin source. These results provide evidence for BMP shuttling in a bilaterally symmetric cnidarian and suggest that BMP shuttling may have been functional in the potentially bilaterally symmetric cnidarian-bilaterian ancestor.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 24","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adu6347","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144278983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Top-down attention and Alzheimer’s pathology affect cortical selectivity during learning, influencing episodic memory in older adults 自上而下的注意力和阿尔茨海默病病理影响学习过程中的皮质选择性，影响老年人的情景记忆

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-06-13 DOI: 10.1126/sciadv.ads4206

Jintao Sheng, Alexandra N. Trelle, America Romero, Jennifer Park, Tammy T. Tran, Sharon J. Sha, Katrin I. Andreasson, Edward N. Wilson, Elizabeth C. Mormino, Anthony D. Wagner

{"title":"Top-down attention and Alzheimer’s pathology affect cortical selectivity during learning, influencing episodic memory in older adults","authors":"Jintao Sheng, Alexandra N. Trelle, America Romero, Jennifer Park, Tammy T. Tran, Sharon J. Sha, Katrin I. Andreasson, Edward N. Wilson, Elizabeth C. Mormino, Anthony D. Wagner","doi":"10.1126/sciadv.ads4206","DOIUrl":"10.1126/sciadv.ads4206","url":null,"abstract":"<div >Effective memory formation declines in human aging. Diminished neural selectivity—reduced differential responses to preferred versus nonpreferred stimuli—may contribute to memory decline, but its drivers remain unclear. We investigated the effects of top-down attention and preclinical Alzheimer’s disease (AD) pathology on neural selectivity in 166 cognitively unimpaired older participants using functional magnetic resonance imaging during a word-face/word-place associative memory task. During learning, neural selectivity in place- and, to a lesser extent, face-selective regions was greater for subsequently remembered than forgotten events; positively scaled with variability in dorsal attention network activity, within and across individuals; and negatively related to AD pathology, evidenced by elevated plasma phosphorylated Tau<sub>181</sub> (pTau<sub>181</sub>). Path analysis revealed that neural selectivity mediated the effects of age, attention, and pTau<sub>181</sub> on memory. These data reveal multiple pathways that contribute to memory differences among older adults—AD-independent reductions in top-down attention and AD-related pathology alter the precision of cortical representations of events during experience, with consequences for remembering.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 24","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.ads4206","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144278981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heavy potassium isotopes in carbonatites reveal oceanic crust subduction as the driver of deep carbon cycling 碳酸盐岩中的重钾同位素揭示了洋壳俯冲是深部碳循环的驱动因素

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-06-13 DOI: 10.1126/sciadv.adt1023

Zheng-Yu Long, Frédéric Moynier, Baptiste Debret, Kun-Feng Qiu, Wei Dai, Hao-Xuan Sun, Jun Deng, Hervé Bertrand, Kevin Burton, Edward Inglis, Sebastian Tappe

{"title":"Heavy potassium isotopes in carbonatites reveal oceanic crust subduction as the driver of deep carbon cycling","authors":"Zheng-Yu Long, Frédéric Moynier, Baptiste Debret, Kun-Feng Qiu, Wei Dai, Hao-Xuan Sun, Jun Deng, Hervé Bertrand, Kevin Burton, Edward Inglis, Sebastian Tappe","doi":"10.1126/sciadv.adt1023","DOIUrl":"10.1126/sciadv.adt1023","url":null,"abstract":"<div >Carbon cycling between surface and mantle reservoirs is pivotal in fostering habitability of Earth. A critical yet poorly constrained parameter is whether crustal carbon can “survive” devolatilization processes that accompany slab subduction and therefore influence deep carbon budgets. Carbonatites provide a key record to address this important topic. Here, we present high-precision potassium isotope data for a large set of carbonatite samples from both continental and oceanic settings, spanning from 2 billion years ago to the present. Modeling suggests that the heavy potassium isotopic compositions of carbonatites are inherited from their mantle sources, rather than resulting from magmatic and postmagmatic processes. Our results demonstrate a strong link between the subduction of oceanic crust and the recycling of carbonates into the mantle sources of carbonatites. These findings support the hypothesis that subduction of carbonate-bearing altered oceanic crust has been a critical mechanism for transferring carbon into the deep Earth through time.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 24","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adt1023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144278894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AbEpiTope-1.0: Improved antibody target prediction by use of AlphaFold and inverse folding AbEpiTope-1.0：利用AlphaFold和逆折叠改进抗体靶标预测

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-06-13 DOI: 10.1126/sciadv.adu1823

Joakim Nøddeskov Clifford, Eve Richardson, Bjoern Peters, Morten Nielsen

{"title":"AbEpiTope-1.0: Improved antibody target prediction by use of AlphaFold and inverse folding","authors":"Joakim Nøddeskov Clifford, Eve Richardson, Bjoern Peters, Morten Nielsen","doi":"10.1126/sciadv.adu1823","DOIUrl":"10.1126/sciadv.adu1823","url":null,"abstract":"<div >B cell epitope prediction tools are crucial for designing vaccines and disease diagnostics. However, predicting which antigens a specific antibody binds to and their exact binding sites (epitopes) remains challenging. Here, we present AbEpiTope-1.0, a tool for antibody-specific B cell epitope prediction, using AlphaFold for structural modeling and inverse folding for machine learning models. On a dataset of 1730 antibody-antigen complexes, AbEpiTope-1.0 outperforms AlphaFold in predicting modeled antibody-antigen interface accuracy. By creating swapped antibody-antigen complex structures for each antibody-antigen complex using incorrect antibodies, we show that predicted accuracies are sensitive to antibody input. Furthermore, a model variant optimized for antibody target prediction—differentiating true from swapped complexes—achieved an accuracy of 61.21% in correctly identifying antibody-antigen pairs. The tool evaluates hundreds of structures in minutes, providing researchers with a resource for screening antibodies targeting specific antigens. AbEpiTope-1.0 is freely available as a web server and software.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 24","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adu1823","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144278895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0