Science Advances最新文献_第2页

Manipulating the direction of turbulent energy flux via tensor geometry in a two-dimensional flow 利用张量几何在二维流动中操纵湍流能量通量的方向

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-07-25 DOI: 10.1126/sciadv.adv0956

Xinyu Si, Filippo De Lillo, Guido Boffetta, Lei Fang

{"title":"Manipulating the direction of turbulent energy flux via tensor geometry in a two-dimensional flow","authors":"Xinyu Si, Filippo De Lillo, Guido Boffetta, Lei Fang","doi":"10.1126/sciadv.adv0956","DOIUrl":"10.1126/sciadv.adv0956","url":null,"abstract":"<div >In turbulent flows, energy flux, the cornerstone of turbulence theory, refers to the transfer of kinetic energy across different scales of motion. The direction of net energy flux is prescribed by the dimensionality of the fluid system: Energy cascades to smaller scales in three-dimensional flows but to larger scales in two-dimensional (2D) flows. Manipulating energy flux is a formidable task because the energy at any scale is not localized in the physical space. Here, we report a theoretical framework that enables control over energy flux direction. On the basis of this framework, we conducted experiments and direct numerical simulations, producing a 2D turbulence with forward energy flux, contrary to classical expectations. Beyond theory, we discuss how our theoretical framework can have profound applications and implications in natural and engineered systems across length scale ranges from 10<sup>−3</sup> to 10<sup>6</sup> meters, including enhanced mixing of microfluidic devices, biologically generated turbulence, breaking persistent coastal transport barriers, and ocean energy budget.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 30","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adv0956","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real-time signal processing enabled by fused networks on a memristor-based system on a chip 实时信号处理由芯片上基于忆阻器的系统上的融合网络实现

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-07-25 DOI: 10.1126/sciadv.adv3436

Zixu Wang, Wenhao Song, Tong Wang, Zihan Wang, Yichun Xu, Mingyi Rao, Fuxi Cai, Wenbo Yin, Mike Shuo-Wei Chen, Ning Ge, Maxwell Collins, Kangjun Bai, Sabyasachi Ganguli, Michael R. Page, Qing Wu, Linda Katehi, Qiangfei Xia, Miao Hu, J. Joshua Yang

{"title":"Real-time signal processing enabled by fused networks on a memristor-based system on a chip","authors":"Zixu Wang, Wenhao Song, Tong Wang, Zihan Wang, Yichun Xu, Mingyi Rao, Fuxi Cai, Wenbo Yin, Mike Shuo-Wei Chen, Ning Ge, Maxwell Collins, Kangjun Bai, Sabyasachi Ganguli, Michael R. Page, Qing Wu, Linda Katehi, Qiangfei Xia, Miao Hu, J. Joshua Yang","doi":"10.1126/sciadv.adv3436","DOIUrl":"10.1126/sciadv.adv3436","url":null,"abstract":"<div >The von Neumann bottleneck has led to a substantial rise in energy consumption of computing hardware and memory systems, particularly for data-intensive tasks like signal processing. Memristor-based in-memory computing offers an efficient alternative by performing computations within analog memory. Here, we demonstrate real-time signal processing using a fused network that combines the real-time discrete Fourier transform (DFT) and convolutional neural network (CNN) on a memristor-based analog system on a chip (SoC). A 128-by-128 memristor crossbar array performs the DFT on audio signals with a peak signal-to-noise ratio of 33.49 dB, while the following CNN classifies the resulting spectrograms with 94.72% accuracy on the AudioMNIST dataset. In addition, convolution-based edge detection is applied to real-time video frames. The SoC offers substantial energy efficiency improvement over traditional digital systems in signal processing tasks. This work highlights the potential of memristor-based SoCs for efficient real-time signal processing.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 30","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adv3436","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant defense-directed discovery of a natural anti-psoriasis agent targeting GAPDH 植物防御导向的天然抗银屑病剂靶向GAPDH的发现

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-07-25 DOI: 10.1126/sciadv.adw2578

Ting-Ting Zhou, Yu Zheng, Man-Wen Zhang, Li-Hu Gong, Kai Guo, Xiao-Ping He, Yan-Chun Liu, Jonathan Gershenzon, Yan Liu, Sheng-Hong Li

{"title":"Plant defense-directed discovery of a natural anti-psoriasis agent targeting GAPDH","authors":"Ting-Ting Zhou, Yu Zheng, Man-Wen Zhang, Li-Hu Gong, Kai Guo, Xiao-Ping He, Yan-Chun Liu, Jonathan Gershenzon, Yan Liu, Sheng-Hong Li","doi":"10.1126/sciadv.adw2578","DOIUrl":"10.1126/sciadv.adw2578","url":null,"abstract":"<div >Elucidating the ecological functions of natural products in plant adaptive mechanisms is an emerging means of discovering lead compounds. Here, we show an undescribed plant glandular trichome-specific defense sesterterpenoid, leucosceptrine F (leu-F), exhibiting potent anti-inflammatory activity by modulating both innate and adaptive immune responses. Leu-F irreversibly binds to glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a cross-kingdom glycolytic enzyme and a promising therapeutic target in autoimmune diseases. Crystal structure of the GAPDH–leu-F complex reveals the formation of a covalent bond between leu-F and the Cys<sup>152</sup> residue. Leu-F notably attenuated glycolysis and concurrently diminished GAPDH-mediated stabilization of activated protein kinase B (AKT). Both leu-F and the total sesterterpenoid extract of <i>Leucosceptrum canum</i> demonstrated notable therapeutic efficacy and safety in mouse models of psoriasis and experimental autoimmune encephalomyelitis. This study underscores leu-F as a promising lead compound for autoimmune disease treatment and provides a compelling example of drug discovery inspired by chemical ecology.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 30","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adw2578","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep homology of a brachyury cis-regulatory syntax and the evolutionary origin of the notochord 一种短舌顺式调节句法的深层同源性和脊索的进化起源

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-07-25 DOI: 10.1126/sciadv.adw3307

Tzu-Pei Fan, Jun-Ru Lee, Che-Yi Lin, Yi-Chih Chen, Ann E. Cutting, R. Andrew Cameron, Jr-Kai Yu, Yi-Hsien Su

{"title":"Deep homology of a brachyury cis-regulatory syntax and the evolutionary origin of the notochord","authors":"Tzu-Pei Fan, Jun-Ru Lee, Che-Yi Lin, Yi-Chih Chen, Ann E. Cutting, R. Andrew Cameron, Jr-Kai Yu, Yi-Hsien Su","doi":"10.1126/sciadv.adw3307","DOIUrl":"10.1126/sciadv.adw3307","url":null,"abstract":"<div >Expression of <i>brachyury</i> in the notochord is regarded as a chordate novelty and links to the origin of the notochord, yet the evolution of this regulatory control remains unclear. Here, we uncovered a regulatory syntax (named SFZE) consisting of binding sites for four transcription factors in notochord enhancers of chordate <i>brachyury</i> genes. SFZE was also identified in potential <i>brachyury</i> enhancers in various non-chordate animals and even in <i>Capsaspora</i>, a unicellular relative to animals. These non-chordate SFZE-containing enhancers exhibited activity in the zebrafish notochord. Furthermore, the SFZE syntax in a non-chordate confers endoderm activity. Our results indicate the ancient association of SFZE with <i>brachyury</i>, likely predating the origin of animals. The emergence of notochordal <i>brachyury</i> expression could be attributed to co-option of upstream signals acting on the conserved SFZE syntax, which facilitates the origin of the notochord from rudimentary endodermal cells.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 30","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adw3307","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The minimal chemotactic cell 最小的趋化细胞

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-07-25 DOI: 10.1126/sciadv.adx9364

Bárbara Borges-Fernandes, Azzurra Apriceno, Andres Arango-Restrepo, Safa Almadhi, Subhadip Ghosh, Joe Forth, Jorge Pedro López-Alonso, Iban Ubarretxena-Belandia, José Miguel Rubi, Lorena Ruiz-Pérez, Ian Williams, Giuseppe Battaglia

{"title":"The minimal chemotactic cell","authors":"Bárbara Borges-Fernandes, Azzurra Apriceno, Andres Arango-Restrepo, Safa Almadhi, Subhadip Ghosh, Joe Forth, Jorge Pedro López-Alonso, Iban Ubarretxena-Belandia, José Miguel Rubi, Lorena Ruiz-Pérez, Ian Williams, Giuseppe Battaglia","doi":"10.1126/sciadv.adx9364","DOIUrl":"10.1126/sciadv.adx9364","url":null,"abstract":"<div >The movement of cells and microorganisms in response to chemical gradients, chemotaxis, is fundamental to the evolution of myriad biological processes. In this work, we demonstrate that even the simplest cell-like structures are capable of chemotactic navigation. By encapsulating enzymes within lipid vesicles that incorporate a minimal number of membrane pores, we reveal that a solitary vesicle can actively propel itself toward an enzyme substrate gradient. Specifically, vesicles loaded with either glucose oxidase or urease and embedded with corresponding transmembrane proteins were tracked within a microfluidic device under a controlled substrate gradient. Our findings establish that a system comprising only an encapsulated enzyme and a single transmembrane pore is sufficient to initiate chemotaxis. This proof-of-concept model underscores the minimalistic yet powerful nature of cellular navigation mechanisms, providing a previously unknown perspective on the origins and evolution of chemotactic behavior in biological systems.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 30","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adx9364","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parity-forbidden superatomic molecular orbital interaction and aurophilicity induced H─Au bonding in H@Au20 偶对禁止的超原子分子轨道相互作用和亲水性诱导了H@Au20中的H─Au键

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-07-25 DOI: 10.1126/sciadv.adx2053

Chenxi Wan, Xinrui Yang, Zhen Gong, Rui Li, Yulei Shi, Boon K. Teo, Zhigang Wang

{"title":"Parity-forbidden superatomic molecular orbital interaction and aurophilicity induced H─Au bonding in H@Au20","authors":"Chenxi Wan, Xinrui Yang, Zhen Gong, Rui Li, Yulei Shi, Boon K. Teo, Zhigang Wang","doi":"10.1126/sciadv.adx2053","DOIUrl":"10.1126/sciadv.adx2053","url":null,"abstract":"<div >Understanding H─Au interaction is essential for elucidating the stereochemistry and reactivity of gold hydrides. By embedding a hydrogen atom in the central tetrahedral cavity of Au<sub>20</sub>, we identify a distinct form of hydrogen-mediated bonding in a gold cluster. Our results show that the H─Au bonding can be described as a process wherein H─Au interactions compensate for relatively weaker Au─Au bonding driven by intrinsic aurophilic interactions. This phenomenon originates from parity-forbidden coupling of superatomic molecular orbitals under a noncentrosymmetric field, giving rise to the atypical H─Au bonding and a formally neutral interstitial H atom. A detailed analysis of optical properties reveals partial suppression of visible-light absorption and emergence of a more favorable phosphorescent channel, a manifestation of the complex nature of this H─Au bonding. These findings suggest a versatile platform for tuning electronic and optical properties of gold cluster systems through subtle atomic-level manipulation.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 30","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adx2053","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Motifs of human high-frequency oscillations structure processing and memory of continuous audiovisual narratives 人类高频振荡母题结构了连续视听叙事的加工与记忆

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-07-25 DOI: 10.1126/sciadv.adv0986

Akash Mishra, Gelana Tostaeva, Maximilian Nentwich, Elizabeth Espinal, Noah Markowitz, Jalen Winfield, Elisabeth Freund, Sabina Gherman, Marcin Leszczynski, Charles E. Schroeder, Ashesh D. Mehta, Stephan Bickel

{"title":"Motifs of human high-frequency oscillations structure processing and memory of continuous audiovisual narratives","authors":"Akash Mishra, Gelana Tostaeva, Maximilian Nentwich, Elizabeth Espinal, Noah Markowitz, Jalen Winfield, Elisabeth Freund, Sabina Gherman, Marcin Leszczynski, Charles E. Schroeder, Ashesh D. Mehta, Stephan Bickel","doi":"10.1126/sciadv.adv0986","DOIUrl":"10.1126/sciadv.adv0986","url":null,"abstract":"<div >The discrete events of our narrative experience are organized by the neural substrate that underlies episodic memory. This narrative process is segmented into distinct units by event boundaries, which facilitate a replay process that acts to consolidate each event into a narrative memory. High-frequency oscillations (HFOs) may synchronize neural activity during these processes. We use intracranial recordings from participants viewing and freely recalling a continuous, audiovisual stimulus. We find that hippocampal HFOs increase following event boundaries and hippocampal-cortical coincident HFOs (co-HFOs) occur in cortical regions that underlie event segmentation (inferior parietal, precuneus, lateral occipital, and inferior frontal cortices). Event-specific co-HFO patterns that occur during event viewing reoccur following event boundaries for the subsequent three events and during recall. This is consistent with models that support replay as a mechanism for memory consolidation. Therefore, HFOs may coordinate activity across brain regions that facilitate event segmentation, encode memory of discrete events, and bind representations to assemble memory of a coherent, continuous experience.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 30","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adv0986","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The gene regulatory landscape driving mouse gonadal supporting cell differentiation 驱动小鼠性腺支持细胞分化的基因调控景观

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-07-25 DOI: 10.1126/sciadv.adv1885

Isabelle Stévant, Elisheva Abberbock, Meshi Ridnik, Roni Weiss, Linoy Swisa, Christopher R. Futtner, Danielle M. Maatouk, Robin Lovell-Badge, Valeriya Malysheva, Nitzan Gonen

{"title":"The gene regulatory landscape driving mouse gonadal supporting cell differentiation","authors":"Isabelle Stévant, Elisheva Abberbock, Meshi Ridnik, Roni Weiss, Linoy Swisa, Christopher R. Futtner, Danielle M. Maatouk, Robin Lovell-Badge, Valeriya Malysheva, Nitzan Gonen","doi":"10.1126/sciadv.adv1885","DOIUrl":"10.1126/sciadv.adv1885","url":null,"abstract":"<div >Gonadal sex determination relies on tipping a delicate balance involving the activation and repression of several transcription factors and signaling pathways. This is likely mediated by numerous noncoding regulatory elements that shape sex-specific transcriptomic programs. To explore the dynamics of these in detail, we performed paired time series of transcriptomic and chromatin accessibility assays on pre-granulosa and Sertoli cells throughout their development in the embryo, making use of new and existing mouse reporter lines. Regulatory elements were associated with their putative target genes by linkage analysis, and this was complemented and verified experimentally using promoter capture Hi-C. We identified the transcription factor motifs enriched in these regulatory elements along with their occupancy, pinpointing LHX9/EMX2 as potentially critical regulators of ovarian development. Variations in the DNA sequence of these regulatory elements are likely to be responsible for many of the unexplained cases of individuals with differences of sex development.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 30","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adv1885","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Full complex amplitude control of second-harmonic generation via electrically tunable intersubband polaritonic metasurfaces 通过电可调谐子带间极化超表面实现二次谐波产生的完全复杂幅度控制

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-07-25 DOI: 10.1126/sciadv.adw8852

Jaeyeon Yu, Jaesung Kim, Hyeongju Chung, Jeongwoo Son, Gerhard Boehm, Mikhail A. Belkin, Jongwon Lee

{"title":"Full complex amplitude control of second-harmonic generation via electrically tunable intersubband polaritonic metasurfaces","authors":"Jaeyeon Yu, Jaesung Kim, Hyeongju Chung, Jeongwoo Son, Gerhard Boehm, Mikhail A. Belkin, Jongwon Lee","doi":"10.1126/sciadv.adw8852","DOIUrl":"10.1126/sciadv.adw8852","url":null,"abstract":"<div >Nonlinear intersubband polaritonic metasurfaces based on coupling of the intersubband nonlinear optical response of quantum-engineered semiconductor heterostructures and electromagnetic modes of nanoresonators provide efficient frequency mixing with moderate pump intensities. The resonant nonlinear optical response, represented as a complex function, can be modulated via Stark tuning of intersubband transition energies under applied voltages. However, achieving full complex amplitude control (both phase and magnitude) remains challenging. In this work, we present and experimentally validate electrically tunable nonlinear intersubband polaritonic metasurfaces that achieve complete complex amplitude control for second-harmonic generation (SHG). Through a design featuring two in-plane flipped meta-atoms per unit cell, we achieve complete electrical control of both the amplitude and phase of the metasurface second-order nonlinear susceptibility, with a tuning range of 0 to 30 nm V<sup>−1</sup> for the magnitude and 0-2π for the phase of the nonlinear optical response. Using these properties, we achieve complete on-off SHG modulation and beam diffraction tuning through electrically controlled amplitude and phase gratings.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 30","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adw8852","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PELP1 coordinates the modular assembly and enzymatic activity of the rixosome complex PELP1协调复合体的模块组装和酶活性

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-07-25 DOI: 10.1126/sciadv.adw4603

Jacob Gordon, Andrea M. Kaminski, Saisamhita R. Bommu, Aleksandra Skrajna, Robert M. Petrovich, Lars C. Pedersen, Robert K. McGinty, Alan J. Warren, Robin E. Stanley

{"title":"PELP1 coordinates the modular assembly and enzymatic activity of the rixosome complex","authors":"Jacob Gordon, Andrea M. Kaminski, Saisamhita R. Bommu, Aleksandra Skrajna, Robert M. Petrovich, Lars C. Pedersen, Robert K. McGinty, Alan J. Warren, Robin E. Stanley","doi":"10.1126/sciadv.adw4603","DOIUrl":"10.1126/sciadv.adw4603","url":null,"abstract":"<div >The rixosome is a large multisubunit complex that initiates RNA decay during critical nuclear transactions including ribosome assembly and heterochromatin maintenance. The overall architecture of the complex remains undefined because several subunits contain intrinsically disordered regions (IDRs). Here, we combined structural and functional approaches to establish PELP1 as the central scaffold of the rixosome upon which the enzymatic subunits modularly assemble. The C-terminal half of PELP1 is composed of a proline-rich IDR that mediates association with the AAA-ATPase MDN1, histones, and the SUMO-specific protease SENP3. The PELP1 IDR contains a glutamic acid–rich region that we establish can chaperone the histone octamer in vitro. Last, the x-ray structure of a small linear motif (SLiM) from the PELP IDR bound to SENP3 reveals how PELP1 allosterically activates SUMO protease activity. This work provides an integrated structural model for understanding the rixosome’s dynamic architecture and how it modularly coordinates several cellular functions.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 30","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adw4603","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0