Science Advances最新文献

{"title":"Erratum for the Research Article \"A splenic-targeted versatile antigen courier: iPSC wrapped in coalescent erythrocyte-liposome as tumor nanovaccine\" by Y. Zhai <i>et al</i>.","authors":"","doi":"10.1126/sciadv.aei1515","DOIUrl":"10.1126/sciadv.aei1515","url":null,"abstract":"","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"12 19","pages":"eaei1515"},"PeriodicalIF":12.5,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13155331/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147857166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The pleuroparenchymal fibroelastosis atlas reveals aberrant cell states and their zonation as an alternate roadmap to lung fibrosis","authors":"Jannik Ruwisch,&nbsp;Aurélie Cazes,&nbsp;Lena M. Leiber,&nbsp;Raphaël Borie,&nbsp;Lavinia Neubert,&nbsp;Leonard Christian,&nbsp;Vincent Thomas de Montpréville,&nbsp;Adam Szmul,&nbsp;Farah Moussa,&nbsp;Stijn E. Verleden,&nbsp;Svenja Gaedcke,&nbsp;Jan Hegermann,&nbsp;Jan Fuge,&nbsp;Matthias Ballmaier,&nbsp;Jan C. Kamp,&nbsp;Mark Greer,&nbsp;Peter Braubach,&nbsp;Christopher Werlein,&nbsp;Fabius Ius,&nbsp;Theresa Graalmann,&nbsp;Kalil Aburahma,&nbsp;Laurens J. De Sadeleer,&nbsp;Ryoko Egashira,&nbsp;Maximilian Ackermann,&nbsp;Daisuke Yamada,&nbsp;Marius M. Hoeper,&nbsp;Christine Falk,&nbsp;Jens Gottlieb,&nbsp;Herbert B. Schiller,&nbsp;Bart Vanaudenaerde,&nbsp;Benjamin Seeliger,&nbsp;Marie-Pierre Debray,&nbsp;Jean Francois Bernaudin,&nbsp;Lars Knudsen,&nbsp;Emmanuel Bergot,&nbsp;Joseph Jacob,&nbsp;Hervé Mal,&nbsp;Danny Jonigk,&nbsp;Sabine Dettmer,&nbsp;Pierre Mordant,&nbsp;Antje Prasse,&nbsp;Elie Fadel,&nbsp;Wim A. Wuyts,&nbsp;Bruno Crestani,&nbsp;Naftali Kaminski,&nbsp;Aurélien Justet,&nbsp;Jonas C. Schupp","doi":"","DOIUrl":"","url":null,"abstract":"<div >Pleuroparenchymal fibroelastosis (PPFE) is a progressive interstitial lung disease (ILD) with defining histology of intra-alveolar fibrosis with septal elastosis (AFE), suggesting unique cellular disease processes. Here, we present a binational single-nucleus RNA sequencing atlas of PPFE, based on explanted lungs from 40 patients. Immunofluorescence microscopy, RNA in situ hybridization, micro–computed tomography (CT), and hierarchical phase-contrast (HiP) synchrotron CT provided spatial context. We identify PPFE-associated adventitial and elastofibrotic fibroblasts as key drivers of elastotic remodeling within an inflammatory microenvironment, maintained by immune cells forming tertiary lymphoid structures. Spatial mapping reveals an intriguing zonation of AFE, maintained by intercellular circuits between PPFE-associated cell types. Comparative analysis with idiopathic pulmonary fibrosis highlights <i>CTHRC1</i>+ fibrotic fibroblasts and aberrant basaloid cells as conserved profibrotic cellular machinery mediating collagen deposition across ILDs. This integrative atlas defines the cellular landscape of PPFE and dissects elastotic from fibrotic remodeling, providing a molecular rationale for niche-specific therapeutic strategies.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"12 19","pages":""},"PeriodicalIF":12.5,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147830238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antigen spreading mediates heterogeneous solid tumor eradication by DNA demethylating agent–programmed CAR T cells","authors":"Yelei Guo,&nbsp;Chuan Tong,&nbsp;Jianshu Wei,&nbsp;Zhiqiang Wu,&nbsp;Yuting Lu,&nbsp;Fuxin Han,&nbsp;Yipeng Zhang,&nbsp;Chunmeng Wang,&nbsp;Jinhong Shi,&nbsp;Fengxia Shi,&nbsp;Yao Wang,&nbsp;Weidong Han","doi":"","DOIUrl":"","url":null,"abstract":"<div >Antigen heterogeneity substantially limits the efficacy of chimeric antigen receptor–modified T (CAR T) cell therapy against solid tumors. Our study highlights the potent antitumor activity of low-dose decitabine-primed CAR T (dCAR T) cells in solid tumor models, a benefit previously confirmed in hematologic malignancies. Notably, dCAR T cell infusion in immunocompetent mice led to substantial elimination of mixed tumor masses containing both antigen-positive and antigen-negative cells, without the need for prior lymphodepletion. Our analysis showed notable proinflammatory remodeling of the tumor immunosuppressive microenvironment. Crucially, antigen-activated dCAR T cells sustained high levels of interferon-γ production, which induced immunogenic cell death in tumor cells and activated conventional dendritic cells. This, in turn, stimulated endogenous CD8⁺ T cells, enhancing their antigen-spreading capacity and aiding in the clearance of abscopal antigen-negative tumors. These findings reveal the robust antigen-spreading capability of dCAR T cells, underscoring their clinical potential in addressing solid tumors with inherent antigen heterogeneity.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"12 19","pages":""},"PeriodicalIF":12.5,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147830220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DLP-bioprinted ultrabiomimetic trachea with spatiotemporal angiogenesis regulation for segmental airway reconstruction","authors":"Jingming Gao,&nbsp;Bohui Li,&nbsp;Zheng Ci,&nbsp;Xingqi Song,&nbsp;Jie Zhu,&nbsp;Liang Duan,&nbsp;Xiaoyun Wang,&nbsp;Yu Liu,&nbsp;Dong Lei,&nbsp;Guangdong Zhou","doi":"","DOIUrl":"","url":null,"abstract":"<div >Segmental airway reconstruction requires tracheal grafts that emulate the hierarchical structure and biochemical functions of native trachea. However, achieving both biomimetic fidelity and efficient vascularization remains challenging. Here, we present a digitally light-processed tissue-engineered trachea featuring anatomically biomimetic C-shaped cartilage rings, alternating fibrous rings, and dorsal fibrous bands, precisely replicating the structural hierarchy of native trachea. To promote rapid neovascularization, we develop a stress-relaxing and degradable alginate-based hydrogel capable of dynamic vascular endothelial growth factor loading and sustained release, thereby facilitating endothelial cell migration and angiogenesis. Within the fibrous regions, pre-engineered vascular channels are incorporated to guide host vascular ingrowth, resulting in a 2.6-fold increase in neovascular density compared to no-channel scaffolds. This platform integrates spatial control through precise structural design with temporal bioactive signal modulation, enabling synchronized vascularization. When transplanted via end-to-end anastomosis with native tracheae, the vascularized grafts exhibit enhanced survival and functional integration, offering a robust strategy for tracheal tissue engineering and segmental airway reconstruction.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"12 19","pages":""},"PeriodicalIF":12.5,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147830224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A balance between nucleating and elongating actin filaments controls deformation of protein condensates.","authors":"Caleb Walker, Daniel Mansour, Unyime M Effiong, Dominique Jordan, Liping Wang, Eileen M Lafer, José Alvarado, Brian Belardi, Padmini Rangamani, Jeanne C Stachowiak","doi":"10.1126/sciadv.aea0196","DOIUrl":"10.1126/sciadv.aea0196","url":null,"abstract":"<p><p>Protein condensates assemble actin filaments into diverse architectures, reminiscent of filopodia and stress fibers. During assembly, filament nucleation and elongation compete for a shared pool of actin monomers. Here we show that a balance between these processes is required to deform condensates of VASP, an actin polymerase, into high aspect ratio structures. Adding magnesium, which promotes filament nucleation, enhanced condensate deformation, while adding profilin, which favors elongation over nucleation, produced ring-like filament bundles that failed to deform condensates. Computational modeling predicted that a collection of short filaments would be more efficient at deforming condensates compared to a few long filaments. To test this prediction, filament capping protein was used to inhibit growth of long filaments. The resulting set of shorter filaments regained the ability to deform condensates, illustrating the importance of a balance between filament nucleation and elongation. More broadly, these findings illustrate how protein condensates balance actin nucleation, elongation, and bundling to direct the assembly of diverse cytoskeletal architectures.</p>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"12 19","pages":"eaea0196"},"PeriodicalIF":12.5,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13155290/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147857018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural noncoding <i>pumilio</i> variants retune value-coding interneurons to bias <i>Drosophila</i> oviposition decisions.","authors":"Dorsa Motevalli, Robert Alfredson, Sydney Fogleman, Emmanuel Medrano, Yang Chen, William I Silander, Toshihide Hige, Ulrich Stern, Chung-Hui Yang","doi":"10.1126/sciadv.aed1338","DOIUrl":"10.1126/sciadv.aed1338","url":null,"abstract":"<p><p>How natural regulatory genetic variation creates innate biases in economic decisions through modifying circuit structure and function is rarely explored. Here, we trace this link in a simple value-based decision in <i>Drosophila</i>: where to oviposit. Although laboratory flies (<i>w¹¹¹⁸</i>) reject sucrose for a plain option, a wild-caught African strain accepts sucrose. This decision difference maps to three African-specific intronic SNPs in <i>pumilio</i> (<i>pum</i>), an RNA binding translational repressor. These SNPs down-regulate <i>pum</i>, derepressing its target, the sodium channel <i>paralytic</i> (<i>para</i>), in a pair of GABAergic interneurons that encode option value. Elevated <i>para</i> boosts neuronal excitability, compresses value contrast between sucrose and plain options, and promotes sucrose acceptance. Selectively reducing <i>pum</i> or overexpressing <i>para</i> in these neurons converts the laboratory flies' physiology and behavior to the African phenotype. These findings offer a genome-to-circuit-to-behavior framework, revealing how subtle regulatory polymorphisms fine-tune circuit properties to diversify environment-appropriate decision biases in nature.</p>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"12 19","pages":"eaed1338"},"PeriodicalIF":12.5,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13155294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147857264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial reorganization of object representations in high-level visual cortex distinguishes working memory from perception.","authors":"Wanru Li, Jia Yang, Pinglei Bao","doi":"10.1126/sciadv.aea7764","DOIUrl":"10.1126/sciadv.aea7764","url":null,"abstract":"<p><p>The visual system balances veridical object perception with flexible visual working memory (VWM), both engaging high-level visual regions. However, how these competing demands shape object-selective cortex (OSC) representations remains unclear. Here, we ask whether VWM inherits the spatial constraints observed in the OSC during perception or reorganizes representations to meet mnemonic demands. Using a matched task design and functional magnetic resonance imaging-based decoding, we systematically compared the localization of object-identity representations during perception and VWM. A robust dissociation emerged: Perception kept object information largely contralateral, whereas VWM produced robust ipsilateral representation even when memorizing bilateral items. These expanded representations exploited more than 90% of ipsilateral OSC vertices and could not be attributed to increased attentional span. Critically, the ipsilateral representations closely mirrored contralateral representations, implicating interhemispheric coordination in VWM. Together, these findings demonstrate that object VWM flexibly recruits distributed high-level visual cortex, with spatial reorganization distinguishing mnemonic flexibility from perceptual fidelity.</p>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"12 19","pages":"eaea7764"},"PeriodicalIF":12.5,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13155342/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147857352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Symmetric instability drives exchange between surface and bottom waters in a coastal front.","authors":"Mareike Körner, Jesse M Cusack, Jonathan Nash, R Kipp Shearman, Leif N Thomas, Jennifer MacKinnon, Fucent Hsuan Wei Hsu, John Taylor, Jinliang Liu, James P Hilditch","doi":"10.1126/sciadv.aeb9841","DOIUrl":"10.1126/sciadv.aeb9841","url":null,"abstract":"<p><p>The coastal ocean in the northern Gulf of Mexico is highly productive and socioeconomically important. Here, stratification can inhibit vertical exchange, with consequences for ecosystem health and hypoxia. Previous work emphasized the role of wind-driven turbulent mixing as a mechanism to overcome the stratification barrier. We identify symmetric instability (SI) as an additional and more energy-efficient pathway linking surface and bottom waters. In high-resolution observations, diagonal bands of overturning motions, a telltale sign of SI, connect the sea surface to the bottom and produce intrusions of temperature and oxygen anomalies. Overturning persists for 2 days after instability-favorable wind ceases. During this time, vertical advective fluxes exceed turbulent fluxes by an order of magnitude, ventilating low-oxygen bottom waters and transporting surface heat downward. Our results show that SI facilitates vertical exchange that can outlive direct wind forcing, highlighting an instability-driven mechanism that may be important in coastal oceans more generally.</p>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"12 19","pages":"eaeb9841"},"PeriodicalIF":12.5,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13155336/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147857391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Universality in peristaltic locomotion: Linking biology, theory, and simulation.","authors":"Roberta Santoriello, Francesco Viola, Vincenzo Citro","doi":"10.1126/sciadv.aec9334","DOIUrl":"10.1126/sciadv.aec9334","url":null,"abstract":"<p><p>Inertial jet-propelled swimmers, such as squids, jellyfishes, and salps, swim through rhythmic body contractions, offering an optimized propulsion mechanism that enables sustained locomotion at low energetic cost. Among the different strategies, the peristaltic propulsion is poorly understood, and a unified view accounting for experimental observations is still missing. This study aims to unveil the fundamental principle behind this type of locomotion, proposing a model swimmer, inspired by salps, that mimics the muscle contractions of real aquatic organisms. Numerical simulations reveal the universality of peristaltic locomotion and allow us to explain experimental data and reconcile biological observations. On the basis of these findings, we develop a simple theoretical framework to predict the system dynamics across different geometric configurations. Beyond its biological relevance, this work shows how the principles of fluid-structure interaction can be translated into predictive models, bridging the gap between the foundations of aquatic jet propulsion and physical laws.</p>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"12 19","pages":"eaec9334"},"PeriodicalIF":12.5,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13155289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147856940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electronics-free soft robotic minitablet for on-demand gastric molecular sensing and diagnostics in vivo.","authors":"Chen Wang, Rui Shi, Anatolii Abalymov, Hao Bao, Thomas Ka-Yam Lam, Zihan Wang, Yongfeng Mei, Zongwei Cai, Xiang-Zhong Chen, Sarthak Misra, Venkatasubramanian K Venkiteswaran","doi":"10.1126/sciadv.aea3309","DOIUrl":"10.1126/sciadv.aea3309","url":null,"abstract":"<p><p>Real-time biomarker sensing and molecular sampling in the stomach can transform how gastrointestinal disorders are diagnosed and managed-yet integrating both capabilities into a single ingestible platform remains a formidable challenge. Existing devices often struggle with size constraints, limited functionality, and the mechanical mismatch between soft, biocompatible materials and rigid electronics. Here, we present SeroTab, an electronics-free soft robotic minitablet that combines real-time pH sensing with on-demand gastric fluid sampling in vivo. Inspired by the gliding motion of penguins through viscous environments, SeroTab features a magnetically actuated, curvature-adaptive body that enables autonomous navigation through complex anatomical geometries to specific gastric regions. Upon reaching its target, a shape memory polymer actuator-triggered wirelessly via radio frequency heating-draws gastric fluid into an internal reservoir (up to 35 microliters). A pH-sensitive hydrogel within the chamber, embedded with biocompatible metal disks, enables ultrasound-based pH readout across a physiologically relevant range (pH 2 to 7) and preserves the sample for untargeted metabolomic profiling. In vivo studies in animal models demonstrate SeroTab's ability to detect pharmacologically induced pH changes (ΔpH = 4, from 2 to 6) and metabolic shifts (42 detected metabolites) following omeprazole administration. By uniting soft robotics, responsive materials, and wireless actuation, SeroTab paves the way for minimally invasive, outpatient-compatible diagnostics that can advance early screening and streamline clinical decision-making.</p>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"12 19","pages":"eaea3309"},"PeriodicalIF":12.5,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13155308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147857126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}