Science Advances最新文献_第10页

Uridine as a potentiator of aminoglycosides through activation of carbohydrate transporters 尿苷通过激活碳水化合物转运体作为氨基糖苷的增强剂

IF 12.5 1区综合性期刊

Science Advances Pub Date : 2025-09-05 DOI: 10.1126/sciadv.adw7630

Manon Lang, Stéphane Renard, Imane El-Meouche, Ariane Amoura, Erick Denamur, Léo Hardy, Julia Bos, Tara Brosschot, Molly A. Ingersoll, Eric Bacqué, Didier Mazel, Zeynep Baharoglu

{"title":"Uridine as a potentiator of aminoglycosides through activation of carbohydrate transporters","authors":"Manon Lang, Stéphane Renard, Imane El-Meouche, Ariane Amoura, Erick Denamur, Léo Hardy, Julia Bos, Tara Brosschot, Molly A. Ingersoll, Eric Bacqué, Didier Mazel, Zeynep Baharoglu","doi":"10.1126/sciadv.adw7630","DOIUrl":"10.1126/sciadv.adw7630","url":null,"abstract":"<div >Aminoglycosides (AGs) are broad-spectrum antibiotics effective against Gram-negative bacteria, with uptake dependent on membrane potential. However, the mechanisms of AG entry remain incompletely understood. Here, we identify a previously undescribed uptake pathway via carbohydrate transporters in <i>E. coli</i>. By deleting or overexpressing 26 carbohydrate transporters, we found that 18 facilitated AG uptake, a mechanism conserved across several Gram-negative ESKAPEE pathogens. Using fluorescent-labeled AGs and flow cytometry, we quantified differential uptake. To enhance AG efficacy, we screened 198 carbon sources for their ability to induce transporter expression using a <i>cmtA</i>-<i>gfp</i> fusion. Uridine emerged as a strong inducer of <i>cmtA</i> and 12 additional AG-importing transporters. Coadministration of uridine considerably improved AG efficacy against clinical and resistant <i>E. coli</i> strains by enhancing drug uptake. This combination also improved outcomes in human blood ex vivo and in a murine urinary tract infection model. Given uridine’s clinical safety, it holds promise as an adjuvant to potentiate AG treatment against multidrug-resistant infections.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 36","pages":""},"PeriodicalIF":12.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adw7630","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TaGW2-TaVOZ1 module regulates wheat salt tolerance via both E3 ligase–dependent and –independent pathways TaGW2-TaVOZ1模块通过E3连接酶依赖性和非依赖性途径调控小麦耐盐性

IF 12.5 1区综合性期刊

Science Advances Pub Date : 2025-09-05 DOI: 10.1126/sciadv.adw3985

Shumin Li, Peiyin Zhang, Xuemin Wang, Wenxuan Feng, Hongqing Zhu, Zhen Qin, Bin Chen, Cun Wang, Xiaojun Nie, Zhensheng Kang, Hude Mao

{"title":"TaGW2-TaVOZ1 module regulates wheat salt tolerance via both E3 ligase–dependent and –independent pathways","authors":"Shumin Li, Peiyin Zhang, Xuemin Wang, Wenxuan Feng, Hongqing Zhu, Zhen Qin, Bin Chen, Cun Wang, Xiaojun Nie, Zhensheng Kang, Hude Mao","doi":"10.1126/sciadv.adw3985","DOIUrl":"10.1126/sciadv.adw3985","url":null,"abstract":"<div >Wheat production is limited by the rapid expansion of salinized arable land worldwide. Identification of the molecular mechanisms that underlie the salt stress response is of great importance. Here, we uncovered the NAC-type transcription factor, TaVOZ1, as a positive regulator of wheat salt tolerance. Its overexpression could enhance yield and biomass production under salt stress, while <i>TaVOZ1</i> knockdown attenuates salt tolerance. TaVOZ1 transcriptionally activates stress-responsive genes, especially <i>HKT1</i>-family transporters, decreasing shoot Na<sup>+</sup> accumulation. However, the RING-type E3 ligase, TaGW2, directly interacts with and ubiquitinates TaVOZ1, promoting its ubiquitin/26<i>S</i> proteasomal degradation. <i>TaGW2</i> overexpression reduces salt tolerance, while its knockdown or knockout enhances wheat response to salt stress. Moreover, we found a moonlight function of TaGW2 wherein it binds the same DNA motifs as TaVOZ1 to block its up-regulation of <i>HKT1</i>-family genes while coordinately governing both the salt tolerance and grain yield. This study highlights the functional versatility of TaGW2 and defines an antagonistic TaGW2-TaVOZ1 regulatory module in wheat salt tolerance.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 36","pages":""},"PeriodicalIF":12.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adw3985","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Near-unity spontaneous emission factor InP surface-emitting lasers based on quasi-bound states in the continuum 基于连续介质中准束缚态的p表面发射激光器的近统一自发发射因子

IF 12.5 1区综合性期刊

Science Advances Pub Date : 2025-09-05 DOI: 10.1126/sciadv.adx6527

Wei Wen Wong, Xiaoying Huang, Olivier Lee Cheong Lem, Chennupati Jagadish, Hark Hoe Tan

{"title":"Near-unity spontaneous emission factor InP surface-emitting lasers based on quasi-bound states in the continuum","authors":"Wei Wen Wong, Xiaoying Huang, Olivier Lee Cheong Lem, Chennupati Jagadish, Hark Hoe Tan","doi":"10.1126/sciadv.adx6527","DOIUrl":"10.1126/sciadv.adx6527","url":null,"abstract":"<div >Surface-emitting lasers featuring optical bound states in the continuum (BICs) have recently emerged as a promising alternative to vertical cavity surface-emitting lasers. However, structural damage caused by top-down fabrication processes remains as a major obstacle that limits device performance. Here, we overcome this bottleneck by demonstrating surface-emitting quasi-BIC lasers fabricated with a bottom-up, etching-free process. We epitaxially grow arrays of crystal phase–engineered InP nanosheets with atomically smooth sidewalls as both the laser active medium and a symmetry-broken quasi-BIC cavity, strategically optimizing coupling between spontaneous emission and the lasing mode. Furthermore, we leverage the dipole selection rule associated with the wurtzite crystal structure of the nanosheets for side mode suppression, achieving single-mode lasing at room temperature with lasing threshold as low as 14 μJ cm<sup>−2</sup> pulse<sup>−1</sup>. Optimal light-cavity coupling is evidenced by spontaneous emission factors as high as 0.8, showcasing the potential of bottom-up BIC lasers in realizing near-thresholdless lasing at room temperature.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 36","pages":""},"PeriodicalIF":12.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adx6527","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unleashing pyroelectricity enhancement via phase transition–driven defect alignment in KNN-based ferroelectrics 利用knn基铁电体的相变驱动缺陷对准释放热释电增强

IF 12.5 1区综合性期刊

Science Advances Pub Date : 2025-09-05 DOI: 10.1126/sciadv.ady5104

Yuntao Huang, Yao Wu, Tiantian Wu, Jianwei Chen, Sijin Wang, Kan-Hao Xue, Zhengqian Fu, Wen Dong, Ting Zheng, Shujun Zhang, Jiagang Wu

{"title":"Unleashing pyroelectricity enhancement via phase transition–driven defect alignment in KNN-based ferroelectrics","authors":"Yuntao Huang, Yao Wu, Tiantian Wu, Jianwei Chen, Sijin Wang, Kan-Hao Xue, Zhengqian Fu, Wen Dong, Ting Zheng, Shujun Zhang, Jiagang Wu","doi":"10.1126/sciadv.ady5104","DOIUrl":"10.1126/sciadv.ady5104","url":null,"abstract":"<div >The pyroelectric effect plays a critical role in thermal imaging and energy harvesting. Despite extensive efforts to enhance performance through doping and composite engineering, the mechanisms underlying defect dipole coupling with phase structures remain poorly understood, impeding the advancement of defect-engineered symmetry modulation. Here, we report an abnormal pyroelectric phenomenon where the pyroelectric coefficient (<i>p</i>) increases notably when poling temperature exceeds the orthorhombic-to-tetragonal phase transition temperature (<i>T</i><sub>O-T</sub>) in potassium sodium niobate ceramics. The <i>p</i> at 200°C (<i>p</i> = 45.4 × 10<sup>−4</sup> C m<sup>−2</sup> K<sup>−1</sup>) rises more than sevenfold compared to poling within the orthorhombic phase (<i>p</i> = 6.5 × 10<sup>−4</sup> C m<sup>−2</sup> K<sup>−1</sup>), representing the highest value reported to date and offering benefit for high-temperature thermal sensing. A dual mechanism is proposed, involving rigid-ion displacement and defect dipole alignment, which respectively contribute to increased displacement charge and space charge. Our findings establish a paradigm for optimizing high-temperature pyroelectrics through a simple, symmetry-confined thermal poling.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 36","pages":""},"PeriodicalIF":12.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.ady5104","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retinal gene therapy using epiretinal AAV-containing fibrin hydrogel implants 含aav纤维蛋白水凝胶植入物的视网膜基因治疗

IF 12.5 1区综合性期刊

Science Advances Pub Date : 2025-09-05 DOI: 10.1126/sciadv.adv7922

Brittni A. Scruggs, Aubrey Berger, Travis Knudsen, Francesca N. Kopp, Matthew Hill, Emma Trncic, Kjersten Anderson, Raymond Iezzi, Alan D. Marmorstein

{"title":"Retinal gene therapy using epiretinal AAV-containing fibrin hydrogel implants","authors":"Brittni A. Scruggs, Aubrey Berger, Travis Knudsen, Francesca N. Kopp, Matthew Hill, Emma Trncic, Kjersten Anderson, Raymond Iezzi, Alan D. Marmorstein","doi":"10.1126/sciadv.adv7922","DOIUrl":"10.1126/sciadv.adv7922","url":null,"abstract":"<div >Subretinal injection of adeno-associated virus (AAV) is generally more efficacious and less inflammatory than intravitreal injection for retinal gene therapy. However, adverse events (e.g., chorioretinal atrophy) have been reported in many patients receiving subretinal injection of Luxturna (voretigene neparvovec-rzyl) and experimental gene therapies. Subretinal injection confines transduction to the treated area. To address this, we manufactured high-concentration fibrin hydrogels encapsulating AAV2–green fluorescent protein (AAV2-<i>GFP</i>). Gels had homogeneous AAV distribution, desired mechanical properties, and retained infectivity. Epiretinal placement of fibrin–AAV2-<i>GFP</i> (<i>n</i> = 11) was compared to subretinal (<i>n</i> = 5) and intravitreal AAV2-<i>GFP</i> injection (<i>n</i> = 3). The subretinal group exhibited inconsistent retinal pigment epithelium (RPE) transduction restricted to the injection region with severe atrophy in two cases. The intravitreal group had weak transduction and inflammation. In contrast, epiretinal hydrogels degraded within days and led to broad transduction of RPE without atrophy or inflammation. This technology could advance gene therapy for retinal degenerations and other ocular or systemic disorders.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 36","pages":""},"PeriodicalIF":12.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adv7922","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bordetellae colonization oligosaccharide (b-Cool), a glycan crucial for nasal colonization 博德tellae定植寡糖（b-Cool），一种对鼻腔定植至关重要的聚糖

IF 12.5 1区综合性期刊

Science Advances Pub Date : 2025-09-03 DOI: 10.1126/sciadv.adw7764

Yang Su, Maiya Callender, Dylan Woolsey, Joanna Zhu, Colleen Sedney, Mimi Thu Vu, Jillian Masters, Amanda Caulfield, Kalyan Dewan, Emily Gaskill, Reilly Stevens, Austin Joshua, Christopher M. Evans, John Glushka, Evgeny Vinogradov, Andrew Preston, Thomas Krunkosky, Maor Bar-Peled, Eric T. Harvill

{"title":"Bordetellae colonization oligosaccharide (b-Cool), a glycan crucial for nasal colonization","authors":"Yang Su, Maiya Callender, Dylan Woolsey, Joanna Zhu, Colleen Sedney, Mimi Thu Vu, Jillian Masters, Amanda Caulfield, Kalyan Dewan, Emily Gaskill, Reilly Stevens, Austin Joshua, Christopher M. Evans, John Glushka, Evgeny Vinogradov, Andrew Preston, Thomas Krunkosky, Maor Bar-Peled, Eric T. Harvill","doi":"10.1126/sciadv.adw7764","DOIUrl":"10.1126/sciadv.adw7764","url":null,"abstract":"<div >Respiratory tract infections pose considerable global health challenges. Upper airway colonization is pivotal to these infections, including those caused by <i>Bordetella</i> species. We identified an oligosaccharide, bordetellae colonization oligosaccharide (b-Cool), crucial for early nasal colonization of <i>Bordetella bronchiseptica</i>. We characterized the structure of b-Cool by LC-MS and NMR and found that it is prevalent across a diverse range of bordetellae, including <i>Bordetella pertussis</i>, which causes whooping cough in humans. A <i>B. bronchiseptica</i> mutant lacking b-Cool (Δ<i>b-Cool</i>) showed significantly delayed and decreased colonization in mouse nasopharynx and nasal epithelia, resulting in decreased transmission. The colonization defect of Δ<i>b-Cool</i> was rescued in mucin deficient mice, suggesting that b-Cool may facilitate colonization in the presence of airway mucins.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 36","pages":""},"PeriodicalIF":12.5,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adw7764","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144935558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ndc80 complex, a conserved coupler for kinetochore-microtubule motility, is a sliding molecular clutch Ndc80复合物是一种滑动的分子离合器，是着丝细胞-微管运动的保守耦合器

IF 12.5 1区综合性期刊

Science Advances Pub Date : 2025-09-03 DOI: 10.1126/sciadv.adx0005

Vladimir M. Demidov, Ivan V. Gonchar, Suvranta K. Tripathy, Fazly I. Ataullakhanov, Ekaterina L. Grishchuk

{"title":"Ndc80 complex, a conserved coupler for kinetochore-microtubule motility, is a sliding molecular clutch","authors":"Vladimir M. Demidov, Ivan V. Gonchar, Suvranta K. Tripathy, Fazly I. Ataullakhanov, Ekaterina L. Grishchuk","doi":"10.1126/sciadv.adx0005","DOIUrl":"10.1126/sciadv.adx0005","url":null,"abstract":"<div >Chromosome motion at spindle microtubule plus ends relies on dynamic molecular bonds between kinetochores and proximal microtubule walls. Under opposing forces, kinetochores move bidirectionally along these walls while remaining near the ends, yet how continuous wall sliding occurs without end detachment remains unclear. Using ultrafast force-clamp spectroscopy, we show that single Ndc80 complexes, the primary microtubule-binding kinetochore component, exhibit processive, bidirectional sliding. Plus end–directed forces induce a mobile catch bond in Ndc80, increasing frictional resistance and restricting sliding toward the tip. Conversely, forces pulling Ndc80 away from the plus end trigger mobile slip-bond behavior, facilitating sliding. This dual behavior arises from force-dependent modulation of the Nuf2 calponin-homology domain’s microtubule binding, identifying this subunit as a friction regulator. We propose that Ndc80’s ability to modulate sliding friction provides the mechanistic basis for the kinetochore’s end coupling, enabling its slip-clutch behavior.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 36","pages":""},"PeriodicalIF":12.5,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adx0005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144935562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cross-feeding percolation phase transitions of intercellular metabolic networks 细胞间代谢网络的交叉摄食渗透相变

IF 12.5 1区综合性期刊

Science Advances Pub Date : 2025-09-03 DOI: 10.1126/sciadv.adv8216

Luís C. F. Latoski, Andrea De Martino, Daniele De Martino

{"title":"Cross-feeding percolation phase transitions of intercellular metabolic networks","authors":"Luís C. F. Latoski, Andrea De Martino, Daniele De Martino","doi":"10.1126/sciadv.adv8216","DOIUrl":"10.1126/sciadv.adv8216","url":null,"abstract":"<div >Intercellular cross-talk is essential for the adaptation capabilities of populations of cells. While direct diffusion-driven cell-to-cell exchanges are difficult to map, current nanotechnology enables one to probe single-cell exchanges with the medium. We introduce a mathematical method to reconstruct the dynamic unfolding of intercellular exchange networks from these data, applying it to an experimental coculture system. The exchange network, initially dense, progressively fragments into small disconnected clusters. To explain these dynamics, we develop a maximum-entropy multicellular metabolic model with diffusion-driven exchanges. The model predicts a transition from a dense network to a sparse one as nutrient consumption shifts. We characterize this crossover both numerically, revealing a power-law decay in the cluster-size distribution, and analytically, by connecting to percolation theory. Comparison with data suggests that populations evolve toward the sparse phase by remaining near the crossover. These findings offer insights into the collective organization driving the adaptive dynamics of cell populations.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 36","pages":""},"PeriodicalIF":12.5,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adv8216","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144935567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flapping-wing robot achieves bird-style self-takeoff by adopting reconfigurable mechanisms 扑翼机器人采用可重构机构，实现了鸟式自起飞

IF 12.5 1区综合性期刊

Science Advances Pub Date : 2025-09-03 DOI: 10.1126/sciadv.adx0465

Ang Chen, Bifeng Song, Kang Liu, Zhihe Wang, Dong Xue, Hongduo Qi

{"title":"Flapping-wing robot achieves bird-style self-takeoff by adopting reconfigurable mechanisms","authors":"Ang Chen, Bifeng Song, Kang Liu, Zhihe Wang, Dong Xue, Hongduo Qi","doi":"10.1126/sciadv.adx0465","DOIUrl":"10.1126/sciadv.adx0465","url":null,"abstract":"<div >Flying vertebrates use specialized wingbeat kinematics in hovering, takeoff, and landing, featuring ventrally anterior downstrokes and aerodynamically inactive upstrokes to enhance aerodynamic characteristics at low airspeeds. Rarely implemented in robotics, this inspired RoboFalcon2.0, a flapping-wing robot with reconfigurable mechanisms performing bioinspired flap-sweep-fold (FSF) motion for controlled bird-style takeoff. FSF couples flapping, sweeping, and folding within a single wingbeat cycle, mimicking vertebrate slow-flight kinematics. Wind tunnel tests demonstrate that sweeping amplitude modulates lift and pitching moment in FSF motion. Computational fluid dynamics simulations reveal that FSF’s aerodynamic effects correlate with leading-edge vortex strength and pressure center location. Dynamics simulations analyze pitch control during takeoff. Real-world flights validate RoboFalcon2.0’s self-takeoff capability. This work advances avian-inspired robotics through vertebrate-like actuation principles, enabling more biomimetic flapping-wing designs.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 36","pages":""},"PeriodicalIF":12.5,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adx0465","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144935535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Galectin-3 induces neurodevelopmental apical-basal polarity and regulates gyrification 半乳糖凝集素-3诱导神经发育的顶基极性并调节旋回

IF 12.5 1区综合性期刊

Science Advances Pub Date : 2025-09-03 DOI: 10.1126/sciadv.adt5859

Luana Campos Soares, Ning Huang, Hana Bernhardova, Viviana Macarelli, Marva Chan, Lara Nikel, Sara Bandiera, Dongnan Yan, Dhanu Gupta, Elisa M. Cruz, Talia Vasaturo-Kolodner, James M. Hillis, Matthew Wood, Mootaz Salman, Zoltán Molnár, Eric O’Neill, Francis G. Szele

{"title":"Galectin-3 induces neurodevelopmental apical-basal polarity and regulates gyrification","authors":"Luana Campos Soares, Ning Huang, Hana Bernhardova, Viviana Macarelli, Marva Chan, Lara Nikel, Sara Bandiera, Dongnan Yan, Dhanu Gupta, Elisa M. Cruz, Talia Vasaturo-Kolodner, James M. Hillis, Matthew Wood, Mootaz Salman, Zoltán Molnár, Eric O’Neill, Francis G. Szele","doi":"10.1126/sciadv.adt5859","DOIUrl":"10.1126/sciadv.adt5859","url":null,"abstract":"<div >Apical-basal polarity (ABP) establishment and maintenance is necessary for proper brain development, yet how it is controlled is unclear. Galectin-3 (Gal-3) has been previously implicated in ABP of epithelial cells, and, here, we find that it is apically expressed in human embryonic stem cells (hESCs) during neural induction. Gal-3 blockade disrupts ABP and alters the distribution of junctional proteins in hESC-derived neural rosettes and is rescued by addition of recombinant Gal-3. Transcriptomics analysis shows that blocking Gal-3 regulates expression of genes responsible for nervous system development and cell junction assembly, among others. Last, Gal-3 blockade during embryonic development in vivo reduces horizontal cell divisions, disturbs cortical layering of neural progenitors, and induces gyrification. These data uncover a regulatory mechanism for ABP in the brain and warrant caution in modulating Gal-3 during pregnancy.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 36","pages":""},"PeriodicalIF":12.5,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adt5859","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144935541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0