Science Advances最新文献_第4页

Ultrabright difuranfluoreno-dithiophen polymers for enhanced afterglow imaging of atherosclerotic plaques

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-03-26 DOI: 10.1126/sciadv.ads4646

Zhe Li, Hui Cao, Youjuan Wang, Shiyi Liao, Xu Li, Baode Chen, Xiaosha Wang, Lihui Jiang, Yingping Zou, Xiao-bing Zhang, Guosheng Song

{"title":"Ultrabright difuranfluoreno-dithiophen polymers for enhanced afterglow imaging of atherosclerotic plaques","authors":"Zhe Li, Hui Cao, Youjuan Wang, Shiyi Liao, Xu Li, Baode Chen, Xiaosha Wang, Lihui Jiang, Yingping Zou, Xiao-bing Zhang, Guosheng Song","doi":"10.1126/sciadv.ads4646","DOIUrl":"10.1126/sciadv.ads4646","url":null,"abstract":"<div >Cardiovascular diseases, including stroke driven by atherosclerosis, remain a leading global health concern. Current diagnostic imaging modalities such as magnetic resonance imaging fail to characterize oxidative stress within atherosclerotic plaques. Here, we introduce difuranfluoreno-dithiophen–based polymers designed for afterglow imaging, offering ultrabright luminescence, ultralow-power excitation (0.087 milliwatts per square centimeter), and ultrashort acquisition times (0.01 seconds). Through a molecular engineering strategy, we have optimized polymers for enhanced reactive oxygen species (ROS) generation capability, ROS capturing capability, and fluorescence quantum yield, resulting in an increase in afterglow intensity (~130-fold) compared to commonly used 2-methoxy-5-(2′-ethylhexyloxy)-1,4-phenylenevinylene polymer (MEHPPV). Additionally, we have developed ratiometric afterglow nanoparticles doped with oxidative stress–responsive molecules, enabling imaging of oxidative stress markers in atherosclerotic plaque. This approach provides a tool for cardiovascular imaging and diagnostics, which is conducive to the auxiliary diagnosis and risk stratification of atherosclerosis.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 13","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.ads4646","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stealth and deception: Adaptive motion camouflage in hunting broadclub cuttlefish

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-03-26 DOI: 10.1126/sciadv.adr3686

Matteo Santon, Jolyon Troscianko, Charlie D. Heatubun, Martin J. How

引用次数: 0

NAT10 and N4-acetylcytidine restrain R-loop levels and related inflammatory responses

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-03-26 DOI: 10.1126/sciadv.ads6144

Turja K. Debnath, Nathan S. Abell, Yi-Ru Li, Sravan K. Devanathan, Enrique Navedo, Blerta Xhemalçe

{"title":"NAT10 and N4-acetylcytidine restrain R-loop levels and related inflammatory responses","authors":"Turja K. Debnath, Nathan S. Abell, Yi-Ru Li, Sravan K. Devanathan, Enrique Navedo, Blerta Xhemalçe","doi":"10.1126/sciadv.ads6144","DOIUrl":"10.1126/sciadv.ads6144","url":null,"abstract":"<div ><i>N</i><sup>4</sup>-acetylcytidine (ac<sup>4</sup>C) is deposited on diverse RNAs by <i>N</i>-acetyltransferase 10 (NAT10), a protein with high biological relevance for aging and cancer. We performed a comprehensive survey of ac<sup>4</sup>C using metabolic labeling, sodium cyanoborohydride chemical treatment coupled to next-generation sequencing (NGS), and ac<sup>4</sup>C antibody–based cell and molecular biology techniques. Our analysis shows that NAT10-dependent ac<sup>4</sup>C-acetylation is robust in rRNA and specific tRNAs but low/spurious in mRNA. It also revealed an inflammatory signature and mutagenesis at transcriptionally active sites in NAT10-KO cells. This finding led us to explore the role of NAT10 in R-loops, which were recently linked to APOBEC3B-mediated mutagenesis. Our analysis showed that R-loops are ac<sup>4</sup>C-acetylated in a NAT10-dependent manner. Furthermore, NAT10 restrains the levels of R-loops at a subset of differentially expressed genes in a catalytic activity–dependent manner. Together with cellular biology data showing ac<sup>4</sup>C-modified RNA in endosomal structures, we propose that increased levels of ac<sup>4</sup>C-unmodified RNAs, likely derived from R-loops, in endosomal structures induce inflammatory responses.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 13","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.ads6144","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring microvascular changes over time with a repositionable 3D ultrasonic capacitive micromachined row-column sensor

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-03-26 DOI: 10.1126/sciadv.adr6449

Cyprien Blanquart, Léa Davenet, Julien Claisse, Mallorie Giroud, Audren Boulmé, Edgard Jeanne, Mickaël Tanter, Mafalda Correia, Thomas Deffieux

{"title":"Monitoring microvascular changes over time with a repositionable 3D ultrasonic capacitive micromachined row-column sensor","authors":"Cyprien Blanquart, Léa Davenet, Julien Claisse, Mallorie Giroud, Audren Boulmé, Edgard Jeanne, Mickaël Tanter, Mafalda Correia, Thomas Deffieux","doi":"10.1126/sciadv.adr6449","DOIUrl":"10.1126/sciadv.adr6449","url":null,"abstract":"<div >eHealth devices, including smartwatches and smart scales, have the potential to transform health care by enabling continuous, real-time monitoring of vital signs over extended periods. Existing technologies, however, lack comprehensive monitoring of the microvascular network, which is linked to conditions such as diabetes, hypertension, and small vessel diseases. This study introduces an ultrasound approach using a capacitive micromachined ultrasound transducer row-column array for continuous, ultrasensitive three-dimensional (3D) Doppler imaging of microvascular changes such as hemodynamic variations or vascular remodeling. In vitro tests and in vivo studies with healthy volunteers demonstrated the sensor’s ability to image the 3D microvascular network at high resolution over different timescales with automatic registration and to detect microvascular changes with high sensitivity. Integrating this technology into wearable devices could, one day, enhance understanding, monitoring, and possibly early detection of microvascular-related health conditions.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 13","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adr6449","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Skin-interfaced multimodal sensing and tactile feedback system as enhanced human-machine interface for closed-loop drone control

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-03-26 DOI: 10.1126/sciadv.adt6041

Chunki Yiu, Yiming Liu, Wooyoung Park, Jian Li, Xingcan Huang, Kuanming Yao, Yuyu Gao, Guangyao Zhao, Hongwei Chu, Jingkun Zhou, Dengfeng Li, Hu Li, Binbin Zhang, Lung Chow, Ya Huang, Qingsong Xu, Xinge Yu

{"title":"Skin-interfaced multimodal sensing and tactile feedback system as enhanced human-machine interface for closed-loop drone control","authors":"Chunki Yiu, Yiming Liu, Wooyoung Park, Jian Li, Xingcan Huang, Kuanming Yao, Yuyu Gao, Guangyao Zhao, Hongwei Chu, Jingkun Zhou, Dengfeng Li, Hu Li, Binbin Zhang, Lung Chow, Ya Huang, Qingsong Xu, Xinge Yu","doi":"10.1126/sciadv.adt6041","DOIUrl":"10.1126/sciadv.adt6041","url":null,"abstract":"<div >Unmanned aerial vehicles have undergone substantial development and market growth recently. With research focusing on improving control strategies for better user experience, feedback systems, which are vital for operator awareness of surroundings and flight status, remain underdeveloped. Current bulky manipulators also hinder accuracy and usability. Here, we present an enhanced human-machine interface based on skin-integrated multimodal sensing and feedback devices for closed-loop drone control. This system captures hand gestures for intuitive, rapid, and precise control. An integrated tactile actuator array translates the drone’s posture into two-dimensional tactile information, enhancing the operator’s perception of the flight situation. Integrated obstacle detection and neuromuscular electrical stimulation–based force feedback system enable collision avoidance and flight path correction. This closed-loop system combines intuitive controls and multimodal feedback to reduce training time and cognitive load while improving flight stability, environmental awareness, and the drone’s posture. The use of stretchable electronics also addresses wearability and bulkiness issues in traditional systems, advancing human-machine interface design.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 13","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adt6041","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Learning the rules of peptide self-assembly through data mining with large language models

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-03-26 DOI: 10.1126/sciadv.adv1971

Zhenze Yang, Sarah K. Yorke, Tuomas P. J. Knowles, Markus J. Buehler

{"title":"Learning the rules of peptide self-assembly through data mining with large language models","authors":"Zhenze Yang, Sarah K. Yorke, Tuomas P. J. Knowles, Markus J. Buehler","doi":"10.1126/sciadv.adv1971","DOIUrl":"10.1126/sciadv.adv1971","url":null,"abstract":"<div >Peptides are ubiquitous and important biomolecules that self-assemble into diverse structures. Although extensive research has explored the effects of chemical composition and exterior conditions on self-assembly, a systematic study consolidating these data to uncover global rules is lacking. In this work, we curate a peptide assembly database through a combination of manual processing by human experts and large language model–assisted literature mining. As a result, we collect over 1000 experimental data entries with information about peptide sequence, experimental conditions, and corresponding self-assembly phases. Using the data, machine learning models are developed, demonstrating excellent accuracy (>80%) in assembly phase classification. Moreover, we fine-tune a GPT model for peptide literature mining with the developed dataset, which markedly outperforms the pretrained model in extracting information from academic publications. This workflow can improve efficiency when exploring potential self-assembling peptide candidates, through guiding experimental work, while also deepening our understanding of the governing mechanisms.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 13","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adv1971","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Astrobiological implications of the stability and reactivity of peptide nucleic acid (PNA) in concentrated sulfuric acid

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-03-26 DOI: 10.1126/sciadv.adr0006

Janusz J. Petkowski, Sara Seager, Maxwell D. Seager, William Bains, Nittert Marinus, Mael Poizat, Chad Plumet, Jim van Wiltenburg, Ton Visser, Martin Poelert

{"title":"Astrobiological implications of the stability and reactivity of peptide nucleic acid (PNA) in concentrated sulfuric acid","authors":"Janusz J. Petkowski, Sara Seager, Maxwell D. Seager, William Bains, Nittert Marinus, Mael Poizat, Chad Plumet, Jim van Wiltenburg, Ton Visser, Martin Poelert","doi":"10.1126/sciadv.adr0006","DOIUrl":"10.1126/sciadv.adr0006","url":null,"abstract":"<div >Recent renewed interest regarding the possibility of life in the Venusian clouds has led to new studies on organic chemistry in concentrated sulfuric acid. However, life requires complex genetic polymers for biological function. Therefore, finding suitable candidates for genetic polymers stable in concentrated sulfuric acid is a necessary first step to establish that biologically functional macromolecules can exist in this environment. We explore peptide nucleic acid (PNA) as a candidate for a genetic-like polymer in a hypothetical sulfuric acid biochemistry. PNA hexamers undergo between 0.4 and 28.6% degradation in 98% (w/w) sulfuric acid at ~25°C, over the span of 14 days, depending on the sequence, but undergo complete solvolysis above 80°C. Our work is the first key step toward the identification of a genetic-like polymer that is stable in this unique solvent and further establishes that concentrated sulfuric acid can sustain a diverse range of organic chemistry that might be the basis of a form of life different from Earth’s.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 13","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adr0006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing huge data from pregnancy natural experiments

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-03-26 DOI: 10.1126/sciadv.adw6818

Michelle Oyen

引用次数: 0

Frontal noradrenergic and cholinergic transients exhibit distinct spatiotemporal dynamics during competitive decision-making

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-03-26 DOI: 10.1126/sciadv.adr9916

Hongli Wang, Heather K. Ortega, Emma B. Kelly, Jonathan Indajang, Neil K. Savalia, Samira Glaeser-Khan, Jiesi Feng, Yulong Li, Alfred P. Kaye, Alex C. Kwan

{"title":"Frontal noradrenergic and cholinergic transients exhibit distinct spatiotemporal dynamics during competitive decision-making","authors":"Hongli Wang, Heather K. Ortega, Emma B. Kelly, Jonathan Indajang, Neil K. Savalia, Samira Glaeser-Khan, Jiesi Feng, Yulong Li, Alfred P. Kaye, Alex C. Kwan","doi":"10.1126/sciadv.adr9916","DOIUrl":"10.1126/sciadv.adr9916","url":null,"abstract":"<div >Norepinephrine (NE) and acetylcholine (ACh) are crucial for learning and decision-making. In the cortex, NE and ACh are released transiently at specific sites along neuromodulatory axons, but how the spatiotemporal patterns of NE and ACh signaling link to behavioral events is unknown. Here, we use two-photon microscopy to visualize neuromodulatory signals in the premotor cortex (medial M2) as mice engage in a competitive matching pennies game. Spatially, NE signals are more segregated with choice and outcome encoded at distinct locations, whereas ACh signals can multiplex and reflect different behavioral correlates at the same site. Temporally, task-driven NE transients were more synchronized and peaked earlier than ACh transients. To test functional relevance, we stimulated neuromodulatory signals using optogenetics to find that NE, but not ACh, increases the animals’ propensity to explore alternate options. Together, the results reveal distinct subcellular spatiotemporal patterns of ACh and NE transients during decision-making in mice.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 13","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adr9916","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Macrophage-targeted Mms6 mRNA-lipid nanoparticles promote locomotor functional recovery after traumatic spinal cord injury in mice

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-03-26 DOI: 10.1126/sciadv.ads2295

Chunyan Fu, Xiaoqin Jin, Kangfan Ji, Ke Lan, Xingjia Mao, Zhaobo Huang, Jian Chen, Fengdong Zhao, Pengfei Li, Xuefei Hu, Liwen Sun, Ning Lu, Jinjie Zhong, Yingying Chen, Linlin Wang

{"title":"Macrophage-targeted Mms6 mRNA-lipid nanoparticles promote locomotor functional recovery after traumatic spinal cord injury in mice","authors":"Chunyan Fu, Xiaoqin Jin, Kangfan Ji, Ke Lan, Xingjia Mao, Zhaobo Huang, Jian Chen, Fengdong Zhao, Pengfei Li, Xuefei Hu, Liwen Sun, Ning Lu, Jinjie Zhong, Yingying Chen, Linlin Wang","doi":"10.1126/sciadv.ads2295","DOIUrl":"10.1126/sciadv.ads2295","url":null,"abstract":"<div >Traumatic spinal cord injury (SCI) causes severe central nervous system damage. M2 macrophages within the lesion are crucial for SCI recovery. Our previous research revealed that M2 macrophages transfected with magnetotactic bacteria–derived <i>Mms6</i> gene can resist ferroptosis and enhance SCI recovery. To address the limitations of M2 macrophage transplantation, we developed lipid nanoparticles (LNPs) encapsulating <i>Mms6</i> mRNA targeting macrophages (<i>Mms6</i> mRNA-PS/LNPs). The targeting efficiency and therapeutic effect of these LNPs in SCI mice were evaluated. Intravenous administration of <i>Mms6</i> mRNA-PS/LNPs delivered more <i>Mms6</i> mRNAs to lesion-site macrophages than those in the <i>Mms6</i> mRNA-LNP group, which resulted in enhancing motor function recovery, reducing lesion area and scar formation, and promoting neuronal survival and nerve fiber repair. These effects were nullified when macrophages were depleted. These findings suggest that macrophage-targeted delivery of <i>Mms6</i> mRNA is a promising therapeutic strategy for promoting spinal cord repair and motor function recovery in patients with traumatic SCI.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 13","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.ads2295","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143703149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0