Science Advances最新文献_第4页

Fast and robust visual object recognition in young children 快速鲁棒的幼儿视觉物体识别

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-07-02 DOI: 10.1126/sciadv.ads6821

Vladislav Ayzenberg, Sukran Bahar Sener, Kylee Novick, Stella F. Lourenco

引用次数: 0

LHX2 regulates dendritic morphogenesis in layer II/III neurons of the neocortex LHX2调节新皮层II/III层神经元的树突形态发生

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-07-02 DOI: 10.1126/sciadv.ado1384

Mahima Bose, Sreenath Ravindran, Sanjna Kumari, Achintya Srivastava, Archana Iyer, Binita Vedak, Ishita Talwar, Rishikesh Narayanan, Shubha Tole

{"title":"LHX2 regulates dendritic morphogenesis in layer II/III neurons of the neocortex","authors":"Mahima Bose, Sreenath Ravindran, Sanjna Kumari, Achintya Srivastava, Archana Iyer, Binita Vedak, Ishita Talwar, Rishikesh Narayanan, Shubha Tole","doi":"10.1126/sciadv.ado1384","DOIUrl":"10.1126/sciadv.ado1384","url":null,"abstract":"<div >In the mammalian neocortex, the two hemispheres communicate via the corpus callosum. We investigated mechanisms regulating dendritic arbors and spines of callosal neurons. The transcription factor LIM Homeodomain 2 (<i>Lhx2</i>), a key regulator of cortical development, is expressed in postmitotic layer II/III neurons and their progenitors. Loss of <i>Lhx2</i> in either population caused similar but distinct phenotypes: reduced dendritic arbors, altered spine morphology, and changed electrophysiological properties. Morphometric defects were more severe when <i>Lhx2</i> was disrupted in progenitors and were recapitulated by its specific loss in basal progenitors. <i>Lhx2</i> loss in progenitors aberrantly up-regulated <i>Neurog2</i> in postmitotic neurons, and <i>Neurog2</i> knockdown partially rescued the phenotype. Loss of <i>Lhx2</i> at either stage also up-regulated Wnt signaling pathway genes. The mutant phenotype was mimicked by constitutive activation of β-CATENIN in postmitotic neurons. Our findings reveal previously unidentified LHX2-dependent mechanisms of dendritic morphogenesis, highlighting its temporally dynamic and diverse roles in neocortical development.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 27","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.ado1384","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnesium microspheres for enhanced transarterial chemoembolization therapy of hepatocellular carcinoma: From animal models to a pilot clinical study 镁微球增强肝细胞癌经动脉化疗栓塞治疗：从动物模型到初步临床研究

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-07-02 DOI: 10.1126/sciadv.adv0885

Fei Gong, Liyun Zheng, Jiachen Xu, Yumin Wu, Qiutong Jin, Jiaqi Lu, Zifan Pei, Zhongwei Zhao, Minjiang Chen, Jianfei Tu, Shiji Fang, Weiqian Chen, Kailu Wei, Nailin Yang, Chuntao Gong, Fazong Wu, Yeyu Zhang, Caifang Ni, Liang Cheng, Zhuang Liu, Jiansong Ji

{"title":"Magnesium microspheres for enhanced transarterial chemoembolization therapy of hepatocellular carcinoma: From animal models to a pilot clinical study","authors":"Fei Gong, Liyun Zheng, Jiachen Xu, Yumin Wu, Qiutong Jin, Jiaqi Lu, Zifan Pei, Zhongwei Zhao, Minjiang Chen, Jianfei Tu, Shiji Fang, Weiqian Chen, Kailu Wei, Nailin Yang, Chuntao Gong, Fazong Wu, Yeyu Zhang, Caifang Ni, Liang Cheng, Zhuang Liu, Jiansong Ji","doi":"10.1126/sciadv.adv0885","DOIUrl":"10.1126/sciadv.adv0885","url":null,"abstract":"<div >Transarterial chemoembolization (TACE) has been extensively used in clinic to treat unresectable hepatocellular carcinoma (HCC). Herein, magnesium microspheres (Mg MSs) were used as embolic devices to enhance lipiodol-mediated TACE. After being dispersed in lipiodol and injected into tumors, Mg MSs would continuously generate hydrogen and magnesium hydroxide, which could neutralize the acidic tumor microenvironment, restore exhausted CD8<sup>+</sup> T cells, reverse immunosuppression, and trigger specific T cell–mediated antitumor responses, synergistically resulting in inhibited tumor growth. As demonstrated in a rabbit orthotopic liver cancer model, artery infusion of Mg MS–dispersed lipiodol offered greatly enhanced therapeutic outcome compared to lipiodol-based or polymeric-bead–based TACE. In a pilot clinical study, among 15 eligible patients with HCC, 11 patients achieved complete response and 3 patients achieved partial responses without unexpected treatment-related adverse events during the 1 to 3 months’ follow-up. The objective response rate of Mg-enhanced TACE was ~93.3% in this small-scale trial, much higher than that of current TACE therapies.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 27","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adv0885","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient and wavelength-tunable second-harmonic generation toward the green gap 面向绿隙的高效波长可调谐二次谐波产生

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-07-02 DOI: 10.1126/sciadv.adw2781

Zhiquan Yuan, Jinhao Ge, Peng Liu, Bohan Li, Mingxiao Li, Jin-Yu Liu, Yan Yu, Hao-Jing Chen, John Bowers, Kerry Vahala

{"title":"Efficient and wavelength-tunable second-harmonic generation toward the green gap","authors":"Zhiquan Yuan, Jinhao Ge, Peng Liu, Bohan Li, Mingxiao Li, Jin-Yu Liu, Yan Yu, Hao-Jing Chen, John Bowers, Kerry Vahala","doi":"10.1126/sciadv.adw2781","DOIUrl":"10.1126/sciadv.adw2781","url":null,"abstract":"<div >Achieving compact and efficient visible laser sources is crucial for a wide range of applications. However, traditional semiconductor laser technology faces difficulties in producing high-brightness green light, leaving a “green gap” in wavelength coverage. Second-harmonic generation (SHG) offers a promising alternative by converting near-infrared sources to visible wavelengths with high efficiency and spectral purity. Here, we demonstrate efficient and tunable SHG within the green spectrum using a high-<i>Q</i> Si<sub>3</sub>N<sub>4</sub> microresonator. On-chip green power as high as 5.3 milliwatts is generated with a conversion efficiency of 141% per watt (absolute 7.9%). A space-charge grating induced by the photogalvanic effect realizes reconfigurable grating numbers and flexible wavelength tuning over a range of 2.6 terahertz. In addition, grating formation dynamics and competition are observed. These findings underscore the potential of Si<sub>3</sub>N<sub>4</sub> as a robust, integrative platform for on-chip, tunable green light sources.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 27","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adw2781","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous two-color imaging with a dual-channel miniscope in freely behaving mice 用双通道显微镜同时对行为自由的小鼠进行双色成像

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-07-02 DOI: 10.1126/sciadv.adr6470

Zhe Dong, Yu Feng, Keziah Diego, Austin M. Baggetta, Brian M. Sweis, Zachary T. Pennington, Sophia I. Lamsifer, Yosif Zaki, Federico Sangiuliano, Paul A. Philipsberg, Denisse Morales-Rodriguez, Daniel Kircher, Paul Slesinger, Tristan Shuman, Daniel Aharoni, Denise J. Cai

{"title":"Simultaneous two-color imaging with a dual-channel miniscope in freely behaving mice","authors":"Zhe Dong, Yu Feng, Keziah Diego, Austin M. Baggetta, Brian M. Sweis, Zachary T. Pennington, Sophia I. Lamsifer, Yosif Zaki, Federico Sangiuliano, Paul A. Philipsberg, Denisse Morales-Rodriguez, Daniel Kircher, Paul Slesinger, Tristan Shuman, Daniel Aharoni, Denise J. Cai","doi":"10.1126/sciadv.adr6470","DOIUrl":"10.1126/sciadv.adr6470","url":null,"abstract":"<div >Miniaturized fluorescence microscopes (miniscopes) enable imaging of calcium events from a large population of neurons in freely behaving animals. Traditionally, miniscopes have only been able to record from a single fluorescence wavelength. Here, we present an open-source dual-channel miniscope that simultaneously records two wavelengths in freely behaving animals. To enable simultaneous acquisition of two fluorescent wavelengths, we incorporated two CMOS sensors into a single miniscope. To validate our dual-channel miniscope, we imaged hippocampal CA1 region that co-expressed a dynamic calcium indicator (GCaMP) and a static nuclear signal (dTomato) while mice ran on a linear track. Our results suggest that, even when neurons were registered across days using dTomato signals, hippocampal spatial coding changes over time. In conclusion, our dual-channel miniscope enables imaging of two fluorescence wavelengths with minimal cross-talk between the two channels, opening the doors to a multitude of previously inaccessible experimental possibilities.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 27","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adr6470","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

All-inorganic metafabric scintillators for conformally flexible and wearable x-ray detection and imaging 用于保形柔性和可穿戴x射线探测和成像的全无机超织物闪烁体

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-06-27

Li Xu, Peng Ran, Wenqian Zhou, Yu Jia, Jianyong Yu, Yang (Michael) Yang, Yang Si

{"title":"All-inorganic metafabric scintillators for conformally flexible and wearable x-ray detection and imaging","authors":"Li Xu, Peng Ran, Wenqian Zhou, Yu Jia, Jianyong Yu, Yang (Michael) Yang, Yang Si","doi":"","DOIUrl":"","url":null,"abstract":"<div >Traditional scintillators rely on rigid inorganic matrices with high-<i>Z</i> elements, whose mechanical inflexibility restricts applications in multiple scenarios. Developing an efficient scintillator that combines inorganic properties with flexibility is a desirable yet highly challenging goal. We pioneered an inorganic metafabric scintillator paradigm through self-sustained slip system engineering, transforming brittle all-inorganic scintillation materials into ductile textile architectures, yielding intrinsically conformally flexible scintillators that adhere seamlessly to complex, curved surfaces. The ultimate all-inorganic scintillator delivers near-unity quantum yield, with scintillation output more than 10 times higher than that of previous polymer matrix–based flexible scintillators. Using these metafabric scintillators, a multimodal x-ray interactive wearable platform (X-Wear) was developed, and their applications in body-centered flexible detection and imaging, mobile health, visual radiation monitoring, and breathable radiation shielding were successfully demonstrated. This work offers a previously undefined paradigm for a scintillator system design strategy that maintains the high performance of inorganic scintillators while adding the functionality of being conformally flexible and wearable of fabrics.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 26","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adv5537","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three-dimensional markerless motion capture of multiple freely behaving monkeys toward automated characterization of social behavior 三维无标记的动作捕捉多个自由行为的猴子对社会行为的自动表征

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-06-27

Jumpei Matsumoto, Takaaki Kaneko, Kei Kimura, Salvador Blanco Negrete, Jia Guo, Naoko Suda-Hashimoto, Akihisa Kaneko, Mayumi Morimoto, Hiroshi Nishimaru, Tsuyoshi Setogawa, Yasuhiro Go, Tomohiro Shibata, Hisao Nishijo, Masahiko Takada, Ken-ichi Inoue

{"title":"Three-dimensional markerless motion capture of multiple freely behaving monkeys toward automated characterization of social behavior","authors":"Jumpei Matsumoto, Takaaki Kaneko, Kei Kimura, Salvador Blanco Negrete, Jia Guo, Naoko Suda-Hashimoto, Akihisa Kaneko, Mayumi Morimoto, Hiroshi Nishimaru, Tsuyoshi Setogawa, Yasuhiro Go, Tomohiro Shibata, Hisao Nishijo, Masahiko Takada, Ken-ichi Inoue","doi":"","DOIUrl":"","url":null,"abstract":"<div >Given their high sociality and close evolutionary distance to humans, monkeys are an essential animal model for unraveling the biological mechanisms underlying human social behavior and elucidating the pathogenesis of diseases exhibiting abnormal social behavior. However, behavioral analysis of naturally behaving monkeys requires manual counting of various behaviors, which has been a bottleneck due to problems in throughput and objectivity. Here, we developed a three-dimensional markerless motion capture system that used multi-view data for robust tracking of individual monkeys and accurate reconstruction of the three-dimensional poses of multiple monkeys living in groups. Validation analysis in two monkey groups revealed that the system enabled the characterization of individual social dispositions and relationships through automated detection of eight basic social events. Analyses of social looking demonstrated its potential for investigating adaptive behaviors in a social group. These results suggest that this motion capture system will greatly enhance our ability to analyze primate social behavior.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 26","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adn1355","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A machine-learning–powered spectral-dominant multimodal soft wearable system for long-term and early-stage diagnosis of plant stresses 一种基于机器学习的多模态软穿戴系统，用于植物逆境的长期和早期诊断

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-06-27

Qin Jiang, Xin Zhao, Tiyong Zhao, Wenlong Li, Jie Ye, Xingxing Dong, Xinyi Wang, Qingyu Liu, Han Ding, Zhibiao Ye, Xiaodong Chen, Zhigang Wu

{"title":"A machine-learning–powered spectral-dominant multimodal soft wearable system for long-term and early-stage diagnosis of plant stresses","authors":"Qin Jiang, Xin Zhao, Tiyong Zhao, Wenlong Li, Jie Ye, Xingxing Dong, Xinyi Wang, Qingyu Liu, Han Ding, Zhibiao Ye, Xiaodong Chen, Zhigang Wu","doi":"","DOIUrl":"","url":null,"abstract":"<div >Addressing the global malnutrition crisis requires precise and timely diagnostics of plant stresses to enhance the quality and yield of nutrient-rich crops, such as tomatoes. Soft wearable sensors offer a promising approach by continuously monitoring plant physiology. However, challenges remain in identifying direct physiological indicators of plant stresses, hindering the development of accurate diagnostic models for predicting symptom progression. Here, we introduce a machine-learning-powered spectral-dominant multimodal soft wearable system (MapS-Wear) for precise, long-term, and early-stage diagnosis of stresses in tomatoes. MapS-Wear continuously tracks leaf surrounding temperature, humidity, and unique in-situ transmission spectra, which are critical stress-related indicators. The machine learning framework processes these multimodal data to predict gradual stress progression and diagnose nutrient deficiencies in plants over 10 days earlier than conventional computer vision methods. Moreover, MapS-Wears enables portable and large-scale screening of grafted tomato varieties in greenhouses, accelerating the identification of compatible grafting combinations. This demonstration highlights the potential for high-throughput plant phenotyping and yield improvement.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 26","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adw7279","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatic NMNAT1 is required to defend against alcohol-associated fatty liver disease 肝脏NMNAT1是防御酒精相关脂肪肝疾病所必需的

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-06-27

Qinchao Ding, Feiwei Cao, Hui Zhuge, Shanglei Lai, Wenjing Cao, Haibin Wei, Rui Guo, Jiannan Qiu, Qing Song, Liuhua Pei, Chaolan Li, Caijuan Si, Zhaoli Sun, Zhenyuan Song, Xiaobing Dou, Songtao Li

{"title":"Hepatic NMNAT1 is required to defend against alcohol-associated fatty liver disease","authors":"Qinchao Ding, Feiwei Cao, Hui Zhuge, Shanglei Lai, Wenjing Cao, Haibin Wei, Rui Guo, Jiannan Qiu, Qing Song, Liuhua Pei, Chaolan Li, Caijuan Si, Zhaoli Sun, Zhenyuan Song, Xiaobing Dou, Songtao Li","doi":"","DOIUrl":"","url":null,"abstract":"<div >Nicotinamide mononucleotide adenylyltransferase 1 (NMNAT1), a nicotinamide adenine dinucleotide (NAD<sup>+</sup>) synthetase in Preiss-Handler and salvage pathways, governs nuclear NAD<sup>+</sup> homeostasis. This study investigated the role of NMNAT1 in alcohol-associated liver disease (ALD). Decreased NMNAT1 expression and activity were observed in the liver of patients with alcohol-associated hepatitis and either liver or primary hepatocytes from ALD mice. F-box and WD repeat domain containing 7 (FBXW7)–regulated interferon regulatory factor 1 (IRF1) ubiquitination degradation contributed to the alcohol-inhibited NMNAT1 transcriptional level. Hepatic NMNAT1 knockout aggravated alcohol-induced hepatic NAD<sup>+</sup> decline and further hepatic steatosis and liver injury. Metabolomics and transcriptomics interaction revealed that the cysteine sulfinic acid decarboxylase (CSAD)–regulated taurine pathway was involved in NMNAT1-disrupted hepatic lipid metabolism in ALD. Hepatic CSAD overexpression or taurine supply attenuated hepatic NMNAT1 knockout–aggravated ALD. Hepatic NMNAT1 loss inhibited NMN-protected ALD. Replenishing hepatic NMNAT1 reversed liver lipid accumulation in ALD mice. These findings identified NMNAT1 as a promising therapeutic target for ALD.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 26","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adt6195","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A PIF-SAUR module safeguards hypocotyl elongation from ABA inhibition in the dark PIF-SAUR模块在黑暗中保护ABA抑制下胚轴伸长

IF 11.7 1区综合性期刊

Science Advances Pub Date : 2025-06-27

Esther Cañibano, Natalia Rodríguez-Sánchez, Daniela Gómez-Soto, Fatima Zahra El Kendi, Jorge Lozano-Juste, Toshinori Kinoshita, Juan Carlos Oliveros, Clara Bourbousse, Sandra Fonseca

{"title":"A PIF-SAUR module safeguards hypocotyl elongation from ABA inhibition in the dark","authors":"Esther Cañibano, Natalia Rodríguez-Sánchez, Daniela Gómez-Soto, Fatima Zahra El Kendi, Jorge Lozano-Juste, Toshinori Kinoshita, Juan Carlos Oliveros, Clara Bourbousse, Sandra Fonseca","doi":"","DOIUrl":"","url":null,"abstract":"<div >Driven by cell elongation, hypocotyl growth is tightly controlled by light and responds to the stress signaling hormone abscisic acid (ABA). However, the molecular connections between ABA and light to control cell elongation are poorly understood. Here, we show that, in Arabidopsis, ABA inhibits hypocotyl elongation in the light but not in the dark. In the dark, hypocotyl sensitivity to ABA is restored in <i>pifq</i> and <i>cop1-4</i> mutants, suggesting that an active light signaling pathway is necessary for hypocotyl responsiveness to ABA. Through an RNA sequencing subtractive approach, we identified ABA differentially expressed genes that correlate with ABA inhibition of hypocotyl elongation, including several <i>SAUR</i> genes. The abrogation of PP2C.D2-SAUR interaction in the <i>pp2c.d2</i>/<i>PP2C.D2<sup>M331K</sup>-GFP</i> line restored ABA sensitivity in the dark, suggesting that SAUR proteins are key to maintain hypocotyls insensitive to ABA. This hypocotyl-specific mechanism enables growth toward the light, overriding ABA inhibition of cell elongation, ensuring subsequent seedling establishment and survival.</div>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 26","pages":""},"PeriodicalIF":11.7,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.science.org/doi/reader/10.1126/sciadv.adv0895","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0