Romanian Biotechnological Letters最新文献_第3页

Purification and characterization of thermo-alkaline stable lipase from Bacillus coagulans and its compatibility with commercially available detergents 凝固芽孢杆菌热碱性稳定脂肪酶的纯化和特性及其与市售洗涤剂的相容性

Romanian Biotechnological Letters Pub Date : 2021-09-20 DOI: 10.25083/rbl/26.5/2994.3001

Abdullah A. Al-Ghanayem

{"title":"Purification and characterization of thermo-alkaline stable lipase from Bacillus coagulans and its compatibility with commercially available detergents","authors":"Abdullah A. Al-Ghanayem","doi":"10.25083/rbl/26.5/2994.3001","DOIUrl":"https://doi.org/10.25083/rbl/26.5/2994.3001","url":null,"abstract":"Thermo-alkaline stable lipase producing B. coagulans was isolated and identified by 16S rRNA sequencing. The lipase production was optimized using different growth parameters. The enzyme was purified and characterized in terms of pH, temperature, solvents, heavy metal ions and inhibitors. Compatibility with commercially available detergents was also studied. The isolate showed maximum lipase production at 37 ⁰C; pH of 9 within 48 h. Addition of magnesium chloride increased lipase production. Sephadex G-100 chromatography was used to purify lipase. The enzyme showed maximum activity at pH 8 of 30 ⁰C. Lipase form B. coagulans was active at a wide range of temperature between 30-70 ⁰C. It was stable in most of the solvents at a concentration 5 and 10%, except dimethyl sulfoxide (DMSO) and methanol. Iodoacetic acid (IAA) and p-chloromercuribenzoic acid (pCMB) had an inhibitory effect on lipase. The lipase was compatible with commercially available detergents that increased the brightness and whiteness of the tested cotton fabrics. Lipase from B. coagulans with alkaline stability at a wide range of temperature has potential application in the detergent industry.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43794884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Fermentation Process for Improving Soy Isoflavones Aglycone Content in Bean Dregs by Lactobacillus plantarum PL70a 植物乳杆菌PL70a提高豆渣中大豆异黄酮苷元含量发酵工艺的优化

Romanian Biotechnological Letters Pub Date : 2021-09-20 DOI: 10.25083/rbl/26.5/2942.2952

Xinyu Heng, Huayou Chen, Jibin Li, Kangtao Cai, Chenxi Lu

引用次数: 0

Production of Puno and Titicaca quinoa cultivars - cost benefit analysis 普诺和的的喀喀岛藜麦品种的生产-成本效益分析

Romanian Biotechnological Letters Pub Date : 2021-09-20 DOI: 10.25083/rbl/26.5/2953.2963

S. Savić, Borisz Czekus, E. Gregoric, Sonja Đuričin, G. Matović

引用次数: 0

Comparative study for obtaining inulinase and invertase by yeasts 酵母制备菊粉酶与转化酶的比较研究

Romanian Biotechnological Letters Pub Date : 2021-09-20 DOI: 10.25083/rbl/26.5/3002.3007

Mariana-Gratiela Soare (Vladu), M. Petrescu, M. Eremia

引用次数: 1

The assessment of microbiological and physicochemical characteristics in drainage waters of the Siriu dam Siriu大坝排水水体微生物及理化特征评价

Romanian Biotechnological Letters Pub Date : 2021-09-20 DOI: 10.25083/rbl/26.5/2979.2985

R. Ruginescu, M. Moldoveanu, Larisa I. Florescu, Ioan Păceșilă, A. Dumitrache, R. Cojoc, S. Neagu, M. Enache

{"title":"The assessment of microbiological and physicochemical characteristics in drainage waters of the Siriu dam","authors":"R. Ruginescu, M. Moldoveanu, Larisa I. Florescu, Ioan Păceșilă, A. Dumitrache, R. Cojoc, S. Neagu, M. Enache","doi":"10.25083/rbl/26.5/2979.2985","DOIUrl":"https://doi.org/10.25083/rbl/26.5/2979.2985","url":null,"abstract":"Based on the balneotherapeutic applications of mineral spring water and particularly sulphurous water, the aim of our research was to study the physicochemical and microbiological parameters of some drainage waters of Siriu dam that showed a strong hydrogen sulphide odour. In addition, due to the corrosive effect of some groups of microorganisms, such as iron-oxidizing bacteria and sulphatereducing bacteria, the present paper also aimed to detect their presence in order to signal the need for some disinfection measures. According to physicochemical analysis, there has been found an external drain that is suitable for use in balneotherapy, presenting a balanced content of mineral elements such as sulphur, calcium, silicon, chlorine and potassium. However, due to the presence of potentially toxic phytoplankton microorganisms such as Microcystis sp. and Phormidium sp. it is necessary to disinfect this water source before using it for any purpose. On the other hand, the identification of both sulphate-reducing bacteria and iron-oxidizing bacteria in the drainage waters of Siriu dam should be considered as an alarm signal as they may lead to bio-corrosion and deterioration of metallic or concrete structures, affecting the integrity of the dam and hydropower constructions.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47425528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Lactic Acid Bacteria in the corn stalk Fermentation by Fluorescence Quantitative PCR 荧光定量PCR法鉴定玉米秸秆发酵过程中的乳酸菌

Romanian Biotechnological Letters Pub Date : 2021-09-20 DOI: 10.25083/rbl/26.5/2926.2935

Huayou Chen, Kangtao Cai, Lingyu Kang, Tingting Li, Xiaoyu Liang, Xinyu Heng

引用次数: 0

Feasibility study of fluorescence quantitative PCR for the detection of microecological dynamics in fermented maize stover feeds 荧光定量PCR检测发酵玉米秸秆饲料微生态动态的可行性研究

Romanian Biotechnological Letters Pub Date : 2021-09-20 DOI: 10.25083/rbl/26.5/2986.2993

Huayou Chen, Chenxi Lu, Tingting Li, Lingyu Kang, Xiaoyu Liang, Zhong Ni

引用次数: 0

Characterization of root-associated bacterial species of Leklek variety of common bean (Phaseolus sp.) 普通豆Leklek (Phaseolus sp.)品种根部伴生细菌种类的鉴定

Romanian Biotechnological Letters Pub Date : 2021-09-20 DOI: 10.25083/rbl/26.5/3008.3013

D. Tekdal, Ilknur Akca, Aslı Küçükrecep, S. Çetiner, R. Hatipoğlu

引用次数: 1

Gene Transformation by Chitosan Nanoparticle to Enhance Fatty Acid Production in Zea mays (L.) 壳聚糖纳米粒基因转化提高玉米脂肪酸产量的研究

Romanian Biotechnological Letters Pub Date : 2021-09-20 DOI: 10.25083/rbl/26.5/2971.2978

E. Hussien, Mohammed Ibrahim Dahab, Kareem Mohammed ABD-ELATTY, Islam Hamdy EL-SHENAWY, H. Farouk, Aya Tarek Rizk, Nada Ahmed Kamal, Rawan Adel ABD-ELWAHAB

{"title":"Gene Transformation by Chitosan Nanoparticle to Enhance Fatty Acid Production in Zea mays (L.)","authors":"E. Hussien, Mohammed Ibrahim Dahab, Kareem Mohammed ABD-ELATTY, Islam Hamdy EL-SHENAWY, H. Farouk, Aya Tarek Rizk, Nada Ahmed Kamal, Rawan Adel ABD-ELWAHAB","doi":"10.25083/rbl/26.5/2971.2978","DOIUrl":"https://doi.org/10.25083/rbl/26.5/2971.2978","url":null,"abstract":"Zea mays is an important crop and an essential source of fatty acids. Hence, increasing and adding new fatty acids led to the enhancement of these properties. Transformation of external Acetyl-CoA gene (Aco) can enhance fatty acid components, as ACo is expressed into Acetyl-CoA carboxylase (ACCase) enzyme, which is the first essential step in the fatty acid production process. Chitosan nanoparticles are safe and fast polymer nanoparticles that are applied for gene transformation. Conventional PCR was performed for the detection of the ACo gene in both transgenic and nontransgenic maize lines. The results confirm the presence of the gene in the transgenic lines and absence in non-transgenic lines. The Gas chromatography-mass spectrometry (GC-MS) analysis for fatty acid contents in transgenic and non-transgenic maize lines showed an increase in fatty acid contents in transgenic lines compared to non-transgenic ones. Besides, the transgenic maize’s lines produced extra new fatty acids not found in non-transgenic ones.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47119925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Screening of local wild-type Xanthomonas spp. for xanthan biosynthesis using media with different carbon sources 利用不同碳源培养基筛选本地野生型黄单胞菌进行黄原胶生物合成

Romanian Biotechnological Letters Pub Date : 2021-06-29 DOI: 10.25083/rbl/26.4/2800-2807

Ida Zahović, J. Dodić, S. Markov, J. Grahovac, M. Grahovac, Zorana Trivunović

引用次数: 2