Romanian Biotechnological Letters最新文献_第10页

Effect of Essential Oils and Hydrosols from Some Selected Lamiaceae Species on Redroot Pigweed (Amaranthus retroflexus L.) 部分Lamiaceae植物精油和水溶胶对红根藜草的影响

Romanian Biotechnological Letters Pub Date : 2021-02-02 DOI: 10.25083/RBL/26.2/2471.2475

Reyyan Yergin Özkan, M. Tunçtürk

{"title":"Effect of Essential Oils and Hydrosols from Some Selected Lamiaceae Species on Redroot Pigweed (Amaranthus retroflexus L.)","authors":"Reyyan Yergin Özkan, M. Tunçtürk","doi":"10.25083/RBL/26.2/2471.2475","DOIUrl":"https://doi.org/10.25083/RBL/26.2/2471.2475","url":null,"abstract":"Allelopathy refers to chemical effect of a plant direct or indirect on germination, growth or development of neighboring plants. Allelopathy can be considered as a component of biological control that reduces the development of other plants. This study was carried out to determine the effect of Greek sage (Salvia fruticosa Miller), basil (Ocimum basilicum L.), Dragonhead (Dracocephalum moldavica L.), spearmint (Menta spicata L.), sage (Salvia officinalis L.), lemon balm (Melissa officinalis L.), oregano (Origanum onites L.) and thyme (Thymus kotschyanus Boiss.) on the germination of redroot pigweed (Amaranthus retroflexus L.) which causes significant yield loss in agricultural production. Essential oil (9, 18, 36 μL/petri) and hydrosols (50, 75, 100%) of aromatic plants were applied to determine their inhibition effects on seed germination of A. retroflexus. The experimental design for in vitro was a randomized design with five replications. It has been shown that germination rate was decreasing by the increased concentration of essential oil and hydrosols of the tested plant species. Also, total germination inhibition of A. retroflexus depended on the essential oil doses; the rate ranged from-2.9 to 85%. Amongst the essential oils, the highest effect was observed in spearmint with 7% germination rate. It could be considered as an important solution, which would contribute in Integrated Weed Management of A. retroflexus by using different concentrations of essential oil and hydrosols from aromatic plants.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":"26 1","pages":"2471-2475"},"PeriodicalIF":0.0,"publicationDate":"2021-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43841582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evolution of humoral immunity effectors in four flocks of sheep with symptoms of contagious ecthyma, from Santău village, Romania 罗马尼亚santure村4群有传染性湿疹症状的绵羊体液免疫效应物的进化

Romanian Biotechnological Letters Pub Date : 2021-02-02 DOI: 10.25083/RBL/26.2/2361.2369

Elisabeta-Rafila Mestesanu

{"title":"Evolution of humoral immunity effectors in four flocks of sheep with symptoms of contagious ecthyma, from Santău village, Romania","authors":"Elisabeta-Rafila Mestesanu","doi":"10.25083/RBL/26.2/2361.2369","DOIUrl":"https://doi.org/10.25083/RBL/26.2/2361.2369","url":null,"abstract":"Contagious ecthyma or contagious pustular dermatitis is a debilitating condition located on the skin of sheep and goats, with a zoonotic character. Contagious ecthyma is a disease with economic and animal welfare implications, rarely being fatal if the host organism suffers from immunosuppression or if secondary infections occur. Similar to other members of the Poxviridae family, immunity to contagious ecthyma is mediated by both innate and adaptive immune responses. Following the invasion of the contagious ecthyma virus, immunoglobulins are mobilized that specifically bind to the contagious ecthyma virus to form immune complexes, which are then eliminated by defense system to protect tissues from damage. The level of circulating immune complexes and immunoglobulins can be quantified and can be an indicator of the stage of infection. In the present study we aimed to investigate whether there is a link between the individual characteristics (sex, age, severity of clinical signs) of sheep with orf symptoms and the evolution of circulating immune complexes and total immunoglobulins. In addition to these investigations, we aimed to follow the evolution of these immunological parameters compared to the evolution in clinically healthy animals. Investigations of the serum revealed that levels of these two parameters (total Ig and CIC), can be influenced by the individual characteristics of sheep affected by the contagious ectima and shows differences compared to clinically healthy animals.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":"26 1","pages":"2361-2369"},"PeriodicalIF":0.0,"publicationDate":"2021-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46000425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic nandrolone decanoate administration effects on antioxidant parameters in Wistar rats 慢性给药癸酸诺龙对Wistar大鼠抗氧化参数的影响

Romanian Biotechnological Letters Pub Date : 2021-02-02 DOI: 10.25083/RBL/26.2/2483.2488

A. Roșca, Bucharest Romania Neuroscience, L. Iosif, Ioan Tivig, R. Mirică, C. Caruntu, C. Timaru, S. Voiculescu, L. Zagrean, I. Stoian

{"title":"Chronic nandrolone decanoate administration effects on antioxidant parameters in Wistar rats","authors":"A. Roșca, Bucharest Romania Neuroscience, L. Iosif, Ioan Tivig, R. Mirică, C. Caruntu, C. Timaru, S. Voiculescu, L. Zagrean, I. Stoian","doi":"10.25083/RBL/26.2/2483.2488","DOIUrl":"https://doi.org/10.25083/RBL/26.2/2483.2488","url":null,"abstract":"Chronic nandrolone decanoate exposure is associated with a modified redox status in the favor of oxidants. Paraoxonase and myeloperoxidase activities are considered sensitive biomarkers of oxidative stress and inflammation, being also predictors for coronary artery disease. The aim of our study was to investigate the impact of chronic nandrolone administration on paraoxonase and myeloperoxidase activities in Wistar rats. 16 Wistar rats were divided into two groups (n=8 for each of them): nandrolone decanoate (A) treated group and control group (C). After 12 weeks of nandrolone decanoate administration we analyzed several plasma oxidative stress parameters: TROLOX Equivalent Antioxidant Activity, paraoxonase and myeloperoxidase activities, total thiols, and advanced oxidation protein products. Our results showed a significant decreased total antioxidant activity in nandrolone treated group compared with controls, while paraoxonase activity and total thiols levels were significantly increased. Myeloperoxidase activity and advanced oxidation protein products were not significantly different between the two studied groups. In conclusion decreased plasma total antioxidant activity suggested increased oxidative stress induced by chronic high doses of nandrolone. Despite oxidative stress presence high doses of nandrolone induced also increased plasma paraoxonase activity.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":"26 1","pages":"2483-2488"},"PeriodicalIF":0.0,"publicationDate":"2021-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42901323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Upsurge of the novel sub-acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic: A systematic review 新型亚急性呼吸综合征冠状病毒2 (SARS-CoV-2)大流行的高潮:系统综述

Romanian Biotechnological Letters Pub Date : 2021-01-01 DOI: 10.25083/rbl/26.1/2195.2206

Rohan Samir Kumar Sachan, A. Upadhyay, A. Dohroo, A. Karnwal

{"title":"An Upsurge of the novel sub-acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic: A systematic review","authors":"Rohan Samir Kumar Sachan, A. Upadhyay, A. Dohroo, A. Karnwal","doi":"10.25083/rbl/26.1/2195.2206","DOIUrl":"https://doi.org/10.25083/rbl/26.1/2195.2206","url":null,"abstract":"The current pandemic has been declared as a Public Health Emergency of International Concern (PHEIC) by the World Health Organization (WHO) on January 30, 2020 The causative agent of the COVID-19 is Sub-Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) that causes lung severe disease, is an enveloped RNA virus The bats being the main zoonotic reservoir of the virus As of July 9, 2020, the number of cases has around 11,841,326 cases, with 544,739 death cases worldwide Surveillance of the asymptomatic carriers, the certain lockdown of the public places, quarantine of 14 days of suspected individuals, or other necessary steps have been taken by each country to limit the coronavirus infection Many scientists have worked on drug repositioning of already available drugs to compete and kill the virus The following literature review has been conducted from the publicly available information about the evolutionary relationship, genetics, pathogenesis, clinical manifestation, diagnosis, or laboratory tests of the virus We have also covered the available control or precautionary measures, and treatment against the virus","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":"26 1","pages":"2195-2206"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69219291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

In vitro evaluation of fungicide and bio-fungicides against isolates of Alternaria solani Sorauer 杀菌剂和生物杀菌剂对茄疫病菌的体外抑菌效果评价

Romanian Biotechnological Letters Pub Date : 2021-01-01 DOI: 10.25083/rbl/26.1/2295.2301

C. Mihăescu, M. Sturzeanu, A. Din

引用次数: 1

Carrier frequencies for thrombophilia-related genetic variants in a Romanian cohort 罗马尼亚队列中与血栓相关的基因变异的携带者频率

Romanian Biotechnological Letters Pub Date : 2021-01-01 DOI: 10.25083/rbl/26.1/2275.2282

Simona Topoleanu, Cristina Mambet, L. Marutescu, F. Ivan, Irina-Alina Cucu, A. Curici, I. Stoica

{"title":"Carrier frequencies for thrombophilia-related genetic variants in a Romanian cohort","authors":"Simona Topoleanu, Cristina Mambet, L. Marutescu, F. Ivan, Irina-Alina Cucu, A. Curici, I. Stoica","doi":"10.25083/rbl/26.1/2275.2282","DOIUrl":"https://doi.org/10.25083/rbl/26.1/2275.2282","url":null,"abstract":"Genetic testing for hereditary thrombophilia, an inherited predisposition to thrombotic events, is increasingly available. To evaluate the rate of positive thrombophilia tests in our laboratory we analyzed the carrier status for common thrombophilia-related gene variants in a consecutive unselected cohort of 360 Romanian patients. Genetic tests were performed on a Real-Time PCR platform. Majority of patients (98.6%) carried at least one thrombophilic variant. The carrier frequencies for classical prothrombotic mutations in F5 (Factor V Leiden) and F2 genes (prothrombin G20210A mutation) were 11.67% (10.27% heterozygous, 1.4% homozygous) and 6.95% (6.39% heterozygous, 0.56% homozygous), respectively. Concurrently, high carrier frequencies for MTHFR c.677C>T, MTHFR c.1298A>C, and PAI-1 4G/5G variants, that are controversially associated with thrombophilia, were observed: 65.28% (52.5% heterozygous, 12.78% homozygous), 53.61% (45% heterozygous, 8.61% homozygous), and 78.61% (49.44% heterozygous, 29.17% homozygous), respectively. The impact of MTHFR genotypes on plasma homocysteine levels was also determined. Male carriers of TT homozygous genotype and CT heterozygous genotype of MTHFR C677T polymorphism had significantly higher levels of plasma homocysteine unrelated to age, compared to those harboring CC homozygous genotype (P=0.028). In unselected patients a high rate of positive thrombophilia tests was observed and the clinical implications of such results need to be carefully examined.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":"26 1","pages":"2275-2282"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69219884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genexpert rapid detection of rifampicin resistance among patients with pulmonary tuberculosis 结核病患者利福平耐药性的基因专家快速检测

Romanian Biotechnological Letters Pub Date : 2021-01-01 DOI: 10.25083/rbl/26.1/2283.2288

G. Popescu, A. Moisoiu, A. Fildan, D. Tofolean, I. Arghir, I. Ion, O. Arghir

{"title":"Genexpert rapid detection of rifampicin resistance among patients with pulmonary tuberculosis","authors":"G. Popescu, A. Moisoiu, A. Fildan, D. Tofolean, I. Arghir, I. Ion, O. Arghir","doi":"10.25083/rbl/26.1/2283.2288","DOIUrl":"https://doi.org/10.25083/rbl/26.1/2283.2288","url":null,"abstract":"The routine conventional phenotypic bacteriological tests available for pulmonary tuberculosis (PTB) diagnosis, such as microscopy by Ziehl Neelsen staining, and Lowenstein Jensen method of solid culture, requires a long time for revealing positivity, resulting in a delayed diagnosis. After Mycobacterium tuberculosis (MTB) genome was discovered, nucleic acid amplification techniques were developed with more rapid detection and identification of rifampicin resistance in respiratory and extra-pulmonary specimens. GeneXpert is a DNA-polymerase chain reaction technique based on NAA, which allows an accurate genotypic method for a quick diagnosis of PTB diagnosis. The aim of the study was to assess retrospectively the yield of genotypic assay GeneXpert in revealing multidrug resistant TB compare to phenotypic confirmation by solid culture. 512 patients with positive and negative smears of suspected PTB were investigated by conventional phenotypic assays. PTB diagnosis assessed by positive genotypic assay and confirmed by phenotypic conventional solid culture method was 78.12%. The yield of GeneXpert in revealing rifampicin resistance was high (n=79/512; 15.43%) with 12.5% additional resistance against isoniazid revealed by positive solid cultures and drug susceptibility test. GeneXpert assay is a very useful method for rapid detection of MTB and drug resistance, which facilitates timely diagnosis and appropriate management of pulmonary tuberculosis.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":"26 1","pages":"2283-2288"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69220516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental approaches targeting the biochemical and antioxidant potential of Synsepalum dulcificum dried fruits 猕猴桃干果生化及抗氧化潜能的实验研究

Romanian Biotechnological Letters Pub Date : 2021-01-01 DOI: 10.25083/rbl/26.1/2230.2235

G. Popa, R. Toma, J. Boe

{"title":"Experimental approaches targeting the biochemical and antioxidant potential of Synsepalum dulcificum dried fruits","authors":"G. Popa, R. Toma, J. Boe","doi":"10.25083/rbl/26.1/2230.2235","DOIUrl":"https://doi.org/10.25083/rbl/26.1/2230.2235","url":null,"abstract":"This study aimed to investigate the biochemical content and antioxidant activity of methanol extracts prepared from Synsepalum dulcificum dried fruits. The results obtained by phytochemical screening of the extracts revealed the presence of some pharmacologically active chemical compounds such as: alkaloids, flavonoids, phenolic compounds and tannins. Quantitative analysis showed that the seeds had phenols in greater amounts (157.88 ± 2.09 mg GAE / g dry matter) than dried fruit pulp (54.09 ± 2.24 mg GAE / g dry matter). Contrariwise, flavonoid content was found to be low in seed (0.013 ± 0.02 mg EQ / g) and high in fruit pulp (0.13 ± 0.05 mg EQ / g). Correlated with the high amount of total phenol, in the seed extracts was found a good antioxidant activity – measured by both DPPH and ferric reducing antioxidant power (FRAP) assays. This antioxidant capacity may be also correlated with the amount of alkaloids, flavonoids, tannins and phenolic compounds, and as well as with their synergistic interactions. Synsepalum dulcificum fruits can be used as a healthy source of additives for the food industry or as natural antioxidants for the pharmaceutical industry.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":"26 1","pages":"2230-2235"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69220091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Sorption of Zinc by exopolysaccharides produced by liquid media of phytopatogenic fungi 植物生真菌液体培养基产生的胞外多糖对锌的吸附

Romanian Biotechnological Letters Pub Date : 2021-01-01 DOI: 10.25083/rbl/26.1/2312.2317

J. Valenzuela-Cobos, A. Grijalva-Endara

引用次数: 2

Clinical and microbiological features of carious dentin in immature permanent molars 未成熟恒磨牙牙本质龋病的临床及微生物学特征

Romanian Biotechnological Letters Pub Date : 2021-01-01 DOI: 10.25083/rbl/26.1/2340.2346

R. Vacaru, S. Per, I. Stanciu, Aneta Munteanu, D. Miricescu, A. Totan, M. Tănase, A. Didilescu

引用次数: 3