Romanian Biotechnological Letters最新文献_第9页

In silico identification of cis-Regulatory elements and their functional annotations from assembled ESTs of Artemisia annua L. involved in abiotic stress signaling 黄花蒿(Artemisia annua L.)非生物胁迫信号通路的顺式调控元件及其功能标记

Romanian Biotechnological Letters Pub Date : 2021-02-02 DOI: 10.25083/RBL/26.2/2384.2395

P. Alam, Thamer Al Balawi

{"title":"In silico identification of cis-Regulatory elements and their functional annotations from assembled ESTs of Artemisia annua L. involved in abiotic stress signaling","authors":"P. Alam, Thamer Al Balawi","doi":"10.25083/RBL/26.2/2384.2395","DOIUrl":"https://doi.org/10.25083/RBL/26.2/2384.2395","url":null,"abstract":"Salt stress is a common side-effect in plants impacted on plant growth, metabolism and productivity. A. annua L. is one of the well-known antimalarial plants, biosynthesized artemisinin in its leaf, now introduced in all-over the world. In this article, we have analyzed the A. annua L. ESTs under salt stress and predicted cis-regulatory elements, roles in abiotic stress signaling. Further, the predicted abiotic stress responsive factors were analyzed in order to their function annotations as compare to the genome of Arabidopsis thaliana. 11 EST-contigs assembled from 127 were 29 signals elements were identified by CAP3 program. In order to evaluate accuracy of the identified factors, gene ontology functions were performed. GOBP analysis enriched the genes (85.71%) as the response to abiotic signaling. The co-expression analysis was revealed by gene investigators and String 10.0, these factors-oriented genes had at least 0.40 correlations and 0.7 mutual connection. In projected PPI network, the recognized factors belong to plant hormone signaling and diterpene pathways. These factors (ABF1, APX CCC1, CPK6, JAZ1, MYC2) introduced as candidate genes responsive factors could be overexpressed in A. annua L. plants either alone or in a shuttle may led the good metabolism and higher artemisinin content.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":"26 1","pages":"2384-2395"},"PeriodicalIF":0.0,"publicationDate":"2021-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47252709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Consistency between Covid-19 RT-PCR and IgM/IgG Quick tests results 新冠肺炎RT-PCR与IgM/IgG快速检测结果的一致性

Romanian Biotechnological Letters Pub Date : 2021-02-02 DOI: 10.25083/RBL/26.2/2560.2565

I. Eremia, Adriana Bidică, R. Nica, E. Radu, C. Cirstoiu, C. Pop, D. Cimponeriu, S. Nica

引用次数: 0

Modelling of ergosterol production by S. cerevisiae in presence of n-dodecane as oxygen-vector 以正十二烷为氧载体，酿酒酵母生产麦角甾醇的模拟

Romanian Biotechnological Letters Pub Date : 2021-02-02 DOI: 10.25083/RBL/26.2/2464.2470

A. Galaction, A. Blaga, Alexandra Tucaliuc, L. Kloetzer, D. Cașcaval

引用次数: 0

Antagonistic Potential of Dairy Origin Enterococcus faecium Against Multidrug-Resistant Foodborne Pathogens 乳制品来源的粪肠球菌对耐多药食源性病原体的拮抗作用

Romanian Biotechnological Letters Pub Date : 2021-02-02 DOI: 10.25083/RBL/26.2/2406.2415

S. Waheed, M. Rasool, B. Aslam, S. Muzammil, M. Waseem, M. Shahid, M. Saqib, S. Hayat, M. Naeem, Zeeshan Taj, S. Kabir, M. Saqalein, M. A. Nisar, M. Khurshid

{"title":"Antagonistic Potential of Dairy Origin Enterococcus faecium Against Multidrug-Resistant Foodborne Pathogens","authors":"S. Waheed, M. Rasool, B. Aslam, S. Muzammil, M. Waseem, M. Shahid, M. Saqib, S. Hayat, M. Naeem, Zeeshan Taj, S. Kabir, M. Saqalein, M. A. Nisar, M. Khurshid","doi":"10.25083/RBL/26.2/2406.2415","DOIUrl":"https://doi.org/10.25083/RBL/26.2/2406.2415","url":null,"abstract":"Probiotic potential of Enterococcus spp. is widely investigated around the globe. The biochemically and molecular characterized E. faecium strains isolated from Dahi (continental yogurt) were evaluated to tolerate simulated gastric environment, bile, sodium chloride, temperature, and pH. The safety was assessed by disc diffusion, broth microdilution, antibiotic resistance genes screening, and hemolytic ability. Enterococci survived simulated gastrointestinal conditions and depicted growth at temperature (15 to ≥42°C), pH (≤2.5 to ≥9.5), 0.3% bile salt and 3% NaCl. All strains were sensitive to ampicillin, vancomycin, kanamycin, gentamicin, streptomycin, tetracycline and ciprofloxacin and harbored vanR, vanX, qnrB2, qnrS, tetK, and tetW resistance genes. E. faecium strains inhibited the E. coli (85%) and S. Typhi (50%) whereas the 10% cell-free culture supernatant (CFCS) of E. faecium halted the growth of E. coli while 15% CFCS completely suppressed S. Typhi. The cell-free culture supernatant retained antibacterial nature after pH and proteinase K treatment, however, it lost activity after heat treatment (≥95°C). The genetic screening revealed that all isolates are capable to produce putrescine biogenic amine. Further assessment of strains for lack of infectivity, cytotoxicity in animals, adhesion to Caco-2 cells and characterization of enterocins is essential to conclude the probiotic potential of these strains.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":"26 1","pages":"2406-2415"},"PeriodicalIF":0.0,"publicationDate":"2021-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48375563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Evaluation of cytotoxicity, nutritional and antioxidative status of lyophilized plant extracts used in dietary supplements 用于膳食补充剂的冻干植物提取物的细胞毒性、营养和抗氧化状态评估

Romanian Biotechnological Letters Pub Date : 2021-02-02 DOI: 10.25083/RBL/26.2/2396.2405

B. Tihauan, I. Marinaș, C. Bleotu, Georgiana Dolete, T. Onisei, Floarea Şerbancea, C. Mateescu, M. Răscol

引用次数: 1

Ex situ preservation in medium-term culture of the threathened taxon Dianthus nardiformis Janka 濒危类群滇石竹中期培养的迁地保护

Romanian Biotechnological Letters Pub Date : 2021-02-02 DOI: 10.25083/RBL/26.2/2416.2422

I. Holobiuc, R. Catană, F. Helepciuc, Carmen Maximilian, M. Mitoi, G. Cogălniceanu

引用次数: 2

Camelina sativa oil can inhibit Campylobacter jejuni but stimulate lactic acid bacteria growth 山茶籽油能抑制空肠弯曲杆菌，但能刺激乳酸菌生长

Romanian Biotechnological Letters Pub Date : 2021-02-02 DOI: 10.25083/RBL/26.2/2423.2427

Ş. Bătrîna, N. Corcionivoschi, Ș. Jurcoane, M. Linton, C. Kelly, Laurette Pinkerton, I. Imbrea, F. Sima, F. Imbrea

引用次数: 2

Isolation and characterization of a thermostable alkaline chitinase-producing Aeromonas strain and its potential in biodegradation of shrimp shell wastes 一株产热稳定碱性几丁质酶的气单胞菌的分离鉴定及其在虾壳废弃物生物降解中的潜力

Romanian Biotechnological Letters Pub Date : 2021-02-02 DOI: 10.25083/RBL/26.2/2511.2522

Zhengang Ma, Jinfeng Tong, Yan Wang, Zeyang Zhou

{"title":"Isolation and characterization of a thermostable alkaline chitinase-producing Aeromonas strain and its potential in biodegradation of shrimp shell wastes","authors":"Zhengang Ma, Jinfeng Tong, Yan Wang, Zeyang Zhou","doi":"10.25083/RBL/26.2/2511.2522","DOIUrl":"https://doi.org/10.25083/RBL/26.2/2511.2522","url":null,"abstract":"Chitinases are employed to the conversion of chitin and are produced by a wide range of bacteria. The objective of this study was to isolate chitinase-producing microorganisms with high chitinolytic activity. A thermostable alkaline chitinase producing isolate strain CQNU6-2 was obtained from soil samples and showed potential in biodegradation of shrimp shell wastes. The optimal culturing conditions of isolate CQNU6-2 is at 25°C and pH 7 for 24 h. The chitinase produced by strain CQNU6-2 exhibited maximum activity at pH 6.0 and 40°C and it could tolerate the treatment of high temperature (up to 80°C) and high pH (over 10). Taxonomic study, based on biochemical and morphological analysis and phylogenetic analysis of 16S rDNA, showed that strain CQNU6-2 was belongs to the genus Aeromonas sp. The isolate can effectively hydrolyze colloidal chitin with degradation rate of 100% and also can directly degrade the shrimp shells. Ammonium sulfate precipitation method can be used to preliminary purify the chitinase. In conclusion, strain CQNU6-2 had a promising potential for biodegradation of chitin under harsh pH or temperature conditions and could be employed to the comprehensive utilization of shrimp shell wastes.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":"26 1","pages":"2511-2522"},"PeriodicalIF":0.0,"publicationDate":"2021-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49549532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Benefits of understanding the enzymatic activities in saline Lake Letea in the Danube Delta 了解多瑙河三角洲Letea盐湖酶活性的好处

Romanian Biotechnological Letters Pub Date : 2021-02-02 DOI: 10.25083/RBL/26.2/2448.2454

Anca Ioana Lucaci, S. Neagu, R. Cojoc, R. Ruginescu, I. Ardelean, M. Enache

引用次数: 0

Antioxidant potential, Total Vitamin C, phenolic and flavonoids content of sweet potato leaves 甘薯叶片的抗氧化能力、总维生素C、酚类和类黄酮含量

Romanian Biotechnological Letters Pub Date : 2021-02-02 DOI: 10.25083/RBL/26.2/2441.2447

M. Cioloca, C. Bădărău, A. Tican, M. Popa

引用次数: 3