Ex situ preservation in medium-term culture of the threathened taxon Dianthus nardiformis Janka

Our aim was to elaborate an efficient and reproducible protocol for medium-term culture of the threatened taxon Dianthus nardiformis. To reduce the growth, sucrose, mannitol, polyethylene glycol, Abscisic acid and Jasmonic acid were tested. For assessing the in vitro response, the growth and regeneration were registered after different time intervals. Mannitol is the most effective for medium-term preservation viable cultures which can be maintained unlimited time through transfer at every 3 months. In its presence, somatic embryogenesis was induced and in vitro growth in the minimal cultures was reduced between 9 and 12 times comparing to the control. Antioxidant enzymes assay revealed qualitative and quantitative differences among the experimental variants, and also between different concentrations of the same compound in correlation with the growth reduction and regeneration. POX was the most suitable to detect the efficiency of different treatments to induce medium-term cultures.