Romanian Biotechnological Letters最新文献

Effect of milling and sourdough fermentation on bioactive compounds of selected multigrain blends 磨粉和酵母发酵对杂粮混合物生物活性成分的影响

Romanian Biotechnological Letters Pub Date : 2021-12-30 DOI: 10.25083/rbl/26.6/3022-3030

I. Banu, C. Neagu, I. Aprodu

引用次数: 0

Incidental Meckel’s diverticula low grade neuroendocrine tumor coexisting with synchronous sigmoidian and perianal adenocarcinoma 偶发性梅克尔憩室低级别神经内分泌肿瘤与同时性乙状结肠腺癌和肛周腺癌并存

Romanian Biotechnological Letters Pub Date : 2021-12-30 DOI: 10.25083/rbl/26.6/3110-3113

S. Păun, I. Tănase, B. Stoica, Cezar Ciubotaru, D. Păun

引用次数: 0

Aflatoxin in feedstuffs and raw milk in Republic of North Macedonia, occurrence and assessment 北马其顿共和国饲料和生乳中黄曲霉毒素的发生和评估

Romanian Biotechnological Letters Pub Date : 2021-12-30 DOI: 10.25083/rbl/26.6/3015-3021

D. Santa, S. Srbinovska, Srekjko Gjorgjievski, Viktorija Gruevska, Frosina Arsovska

{"title":"Aflatoxin in feedstuffs and raw milk in Republic of North Macedonia, occurrence and assessment","authors":"D. Santa, S. Srbinovska, Srekjko Gjorgjievski, Viktorija Gruevska, Frosina Arsovska","doi":"10.25083/rbl/26.6/3015-3021","DOIUrl":"https://doi.org/10.25083/rbl/26.6/3015-3021","url":null,"abstract":"The main objective of this research is to estimate the situation of occurance of aflatoxins in feedstuffs and aflatoxin M1 raw cow`s milk in Republic of North Macedonia. Detected average concentrations of total aflatoxins in the examined period 2014-2017 were below legal prescribed level. Within the official monitoring programme in the country, 1718 samples of raw cow's milk have been analyzed during 2017 and 2018. Comparison of the data showed that the number of examined samples with values for aflatoxin M1 above the allowed limits, according to the regulation is in 2018 (10 samples) and 2017 (5 samples). Additionally, the calculation of exposure assessment of the human population in North Macedonia demonstrated that the exposure to AFM1 of the adult population in N. Macedonia for 2017 is 0.0563 ng/kg bw/daily, and for 2018 is 0.0824 ng/kg bw/daily. The calculated hazard index (HI) In both years, was lower than one, which means that there are no significant health risks for the consumers. It is important to have continuously low levels of aflatoxins in animal feed and nutrition, especially that the climate changes create more favorable conditions for the development of moulds of Aspergillus.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47741704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response of morphological and biochemical traits and transcriptomic of CDPKs and P5CS Genes to phytohormones and nutrients in two bread wheat germplasms under salinity conditions 盐度条件下两种面包小麦种质CDPKs和P5CS基因形态生化特征及转录组学对植物激素和营养物质的响应

Romanian Biotechnological Letters Pub Date : 2021-12-30 DOI: 10.25083/rbl/26.6/3054-3061

E. Hadia, A. Slama, A. Zoghlami, Leila Romdhane, Ahmed Houssein Abodoma, M. Fahej, L. Radhouane

{"title":"Response of morphological and biochemical traits and transcriptomic of CDPKs and P5CS Genes to phytohormones and nutrients in two bread wheat germplasms under salinity conditions","authors":"E. Hadia, A. Slama, A. Zoghlami, Leila Romdhane, Ahmed Houssein Abodoma, M. Fahej, L. Radhouane","doi":"10.25083/rbl/26.6/3054-3061","DOIUrl":"https://doi.org/10.25083/rbl/26.6/3054-3061","url":null,"abstract":"Several enzymes play an important role in the biosynthesis of osmolyte in plants. The main objective of this work is to study the effects of salt stress, kinetin+potassium and giberellic acid+potassium on calcium-dependent protein kinases (CDPKs) and delta-1-pyrroline-5- carboxylate synthase (P5CS) genes expression of two bread wheat varieties. The results shodwe that, salts stress reduced the plant growth of the two wheat germplasms. Moreover, the addition of kinetin+ potassium improved the performance of morphometric parameters while the addition of giberellic acid +potassium has less effect. On the biochemical level, results indicated that salt stress increased the proline contents compared with control plants. Extra increase in proline contents was recorded by kinetin+ potassium, while the treatment of giberellic acid +potassium showed almost similar results as in salt stress only. On the molecular level, salt stress, kinetin and giberellic acid significantly increased the two genes expression of CDPKs and P5CS with more effect in presence of kinetin+ potassium.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44930918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selection of a suitable viral DNA extraction method for Sheeppox virus in cell culture 细胞培养中绵羊痘病毒DNA提取方法的选择

Romanian Biotechnological Letters Pub Date : 2021-12-30 DOI: 10.25083/rbl/26.6/3095-3101

Piyali Mondal, C. L. Patel, Rachna Sagar, Insha Zafir, J. Sonowal, K. Dev, K. Bharali, Neha Singh, B. N, Amitesh Dubey, B. Mishra, B. Mishra

{"title":"Selection of a suitable viral DNA extraction method for Sheeppox virus in cell culture","authors":"Piyali Mondal, C. L. Patel, Rachna Sagar, Insha Zafir, J. Sonowal, K. Dev, K. Bharali, Neha Singh, B. N, Amitesh Dubey, B. Mishra, B. Mishra","doi":"10.25083/rbl/26.6/3095-3101","DOIUrl":"https://doi.org/10.25083/rbl/26.6/3095-3101","url":null,"abstract":"A suitable method for the extraction of nucleic acids should be efficient, sensitive, rapid and simple. Moreover, ideally, good method should yield pure nucleic acid-free from any contaminant inhibitors. Several methods have been reported for viral deoxyribonucleic acid (DNA) isolation but limited information is available on quick and simple isolation of Sheeppox virus (SPPV) genomic DNA in cell culture. In this study, the healthy Vero cells and primary lamb testis cells were infected with SPPV strains such as SPPV-Jaipur, SPPV-Ranipet and SPPV-Roumanian Fanar (RF) and harvested when it exhibited clear cytopathic effect (CPE) in culture. Four different DNA extraction methods i.e., (i) Phenol/chloroform/Isoamyl alcohol method, (ii) Cell lysis buffer method, (iii) Proteinase-k method, and (iv) commercial nucleic acid extraction kit was used to extract optimum yield of viral genomic DNA from clarified culture supernatant of harvested SPPV virus. The DNA sample was characterized using the Nanodrop spectrophotometer and agarose gel electrophoresis. Significantly (p<0.05) higher yield of SPPV genomic DNA was obtained in proteinase-k method which was about 3-5 times more than other methods. Among these methods, proteinase-k protocol was found to be comparatively very effective method in terms of yield of viral genomic DNA, and was free from PCR inhibitors.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48042451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Optimization of Small Peptide Feed from Milk Thistle Residue by Synergistic Fermentation of Multiple Strains and Proteases 多菌株和蛋白酶协同发酵法优化乳蓟渣小肽饲料

Romanian Biotechnological Letters Pub Date : 2021-12-30 DOI: 10.25083/rbl/26.6/3102-3109

Kangtao Cai, Huayou Chen, Xinyu Heng, Lingyu Kang, J. Wu, Chenxi Lu, Xiaoyu Liang

{"title":"Optimization of Small Peptide Feed from Milk Thistle Residue by Synergistic Fermentation of Multiple Strains and Proteases","authors":"Kangtao Cai, Huayou Chen, Xinyu Heng, Lingyu Kang, J. Wu, Chenxi Lu, Xiaoyu Liang","doi":"10.25083/rbl/26.6/3102-3109","DOIUrl":"https://doi.org/10.25083/rbl/26.6/3102-3109","url":null,"abstract":"In order to improve the utilization rate of the milk thistle residue, this study used the synergistic fermentation of multiple strains and proteases to increase the small peptide content of the fermented feed produced by the milk thistle residue. Taking the small peptide content of the milk thistle residue fermented feed as an indicator, the optimal fermentation process was obtained by single-factor optimization experiments and the response surface methodology. The optimal fermentation process was as follows: fermentation time of 7 days, inoculum size of 15%, inoculation ratio of aerobic strains: anaerobic strains = 1: 2, solid-state fermentation water content of 66%, fermentation temperature of 36℃, and amount of protease was 0.25% acid protease+0.25% bromelain. Under the above process, the small peptide content of the fermented feed from milk thistle residue was greatly improved to 57.86%. These results inferred that the added proteases were beneficial to the growth of fermentative microorganisms, the secretion of protease and the increase of the small peptide content.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48951124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of OsDREB2A in Transgenic Tomato Improves Drought Tolerance OsDREB2A基因在番茄中的表达提高了番茄的抗旱性

Romanian Biotechnological Letters Pub Date : 2021-12-30 DOI: 10.25083/rbl/26.6/3145-3154

N. Hassan, N. M. El-Shafey, S. Khodary, H. El-Shabrawi, A. Badr

{"title":"Expression of OsDREB2A in Transgenic Tomato Improves Drought Tolerance","authors":"N. Hassan, N. M. El-Shafey, S. Khodary, H. El-Shabrawi, A. Badr","doi":"10.25083/rbl/26.6/3145-3154","DOIUrl":"https://doi.org/10.25083/rbl/26.6/3145-3154","url":null,"abstract":"Dehydration responsive element binding (DREB) are important regulatory molecules which have a crucial role in abiotic stress tolerance. The productivity of tomato, as a drought-sensitive crop, is highly restricted by drought stress. The current study aimed at introducing the OsDERB2A gene into two tomato genotypes via Agrobacterium-mediated transformation system. Cotyledonary explants were pre-cultured for two days with Agrobacterium strain LBA4404 harboring pCAMBIA1301 with OsDREB2A driven by the constitutive promoter CaMV35S for transformation. Shoots were directly regenerated on MS medium containing 1 mg l-1 zeatin and 1 mg l-1 BAP, and in presence of 30 mg l-1 hygromycin as selective agent. Only eight weeks were needed to regenerate transgenic tomato using this protocol. An OD600 of 0.4 resulted in 64.3-76.9% transformation efficiency. Stable integration and expression of the OsDREB2A gene were confirmed in transgenic tomato using PCR and RT-PCR analyses, and drought tolerance of T0 transgenic lines was confirmed by leaf disc assay in 300 mM mannitol. The superior biomass, photosynthetic pigments, free soluble sugars and proline accumulation of OsDREB2A transgenic lines over wild type in response to mannitol-stress revealed their enhanced drought tolerance and indicated that the constitutive expression of OsDREB2A might modulate the expression of other drought responsive genes.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42682749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detoxification of corn stalk pretreated with calcium hydroxide for true protein accumulation 氢氧化钙预处理玉米秸秆解毒对蛋白质积累的影响

Romanian Biotechnological Letters Pub Date : 2021-12-30 DOI: 10.25083/rbl/26.6/3137-3144

Huayou Chen, Xiaoyu Liang, Kangtao Cai, Bangguo Wu, Hongcheng Wang, Zhong Ni

{"title":"Detoxification of corn stalk pretreated with calcium hydroxide for true protein accumulation","authors":"Huayou Chen, Xiaoyu Liang, Kangtao Cai, Bangguo Wu, Hongcheng Wang, Zhong Ni","doi":"10.25083/rbl/26.6/3137-3144","DOIUrl":"https://doi.org/10.25083/rbl/26.6/3137-3144","url":null,"abstract":"Alkaline pretreatment is essential in lignin degradation, but the inhibitors produced in this process affect microbial growth. To overcome the impacts of the phenolic compounds, detoxification was applied to corn stalk pretreated with calcium hydroxide.The results showed that ferulic acid degradation rate can reach 85.11% by laccase at the optimal conditions. Phanerochaete chrysosporium degraded most vanillin (77.19%) and p-hydroxybenzaldehyde (63.82%) but the degradation of ferulic acid (15.34%) was relatively weak. Laccase combined with Phanerochaete chrysosporium detoxified most of the phenolic compounds including 2- methoxy-4-vinylphenol (88.46%) and salicylic acid (58.13%) that hardly decompose alone after calcium hydroxide pretreatment in this study. These results inferred that Phanerochaete chrysosporium might generate some substance during the spore germination and growth period which may cooperate with laccase to decompose the phenolic compounds. After the fermentation of detoxified corn stalk by Neurospora crassa, the true protein content was increased by 2.73 times, and 21.17% lignin was degraded.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42671701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Online Advanced Bacterial Identification Software, an Original Tool for Phenotypic Bacterial Identification 在线高级细菌鉴定软件，表型细菌鉴定的原始工具

Romanian Biotechnological Letters Pub Date : 2021-12-30 DOI: 10.25083/rbl/26.6/3047-3053

I. Sorescu, Costin Stoica

引用次数: 15

Regulation of the intestinal dysbiosis of high – fat diet obese mice via gut microbia–TLR2 / 4 pathway by electroacupuncture 电针通过肠道微生物- tlr2 / 4通路调节高脂饮食肥胖小鼠肠道生态失调

Romanian Biotechnological Letters Pub Date : 2021-12-30 DOI: 10.25083/rbl/26.6/3155-3165

Yuan-Cheng Si, Chengcheng Ren, Bo Chen, Qing-qing Li, Weiyan Ding

引用次数: 0