Romanian Biotechnological Letters最新文献_第2页

SATB1 enhances gastric cancer progression and metastasis via upregulation of CLIP4/DZIP1/ PRICKLE2 axis in gastric cancer patients SATB1通过上调癌症患者CLIP4/DZIIP1/PRICKLE2轴促进癌症进展和转移

Romanian Biotechnological Letters Pub Date : 2021-12-30 DOI: 10.25083/rbl/26.6/3062-3073

Samina Gul, Amjad Ali, Shahzad Ahmad, Chong‐Yue Zhao, A. Shah, Kashif Rahim, N. Azam, Mohammad Tariq, Yasir Sardar, F. Hussain, Saraj Bahadur, Muhammad Shuaib

{"title":"SATB1 enhances gastric cancer progression and metastasis via upregulation of CLIP4/DZIP1/ PRICKLE2 axis in gastric cancer patients","authors":"Samina Gul, Amjad Ali, Shahzad Ahmad, Chong‐Yue Zhao, A. Shah, Kashif Rahim, N. Azam, Mohammad Tariq, Yasir Sardar, F. Hussain, Saraj Bahadur, Muhammad Shuaib","doi":"10.25083/rbl/26.6/3062-3073","DOIUrl":"https://doi.org/10.25083/rbl/26.6/3062-3073","url":null,"abstract":"SATB1 (Special AT-rich sequence binding protein 1) plays key role in chromatin remodeling and geneexpression. SATB1 has been shown to promote invasion, migration and metastasis. However, the underlying molecular mechanisms, function and clinicopathological features of SATB1 in gastric cancer (GC) remains poorly understood. Here, we show that SATB1 plays critical role in GC progression. SATB1 upregulates in GC patients’ samples and shows co-apmlification with a subset of oncogenic proteins (PRICKLE2, ZEB1, CBWX7, WWTR1, ENAH, DZIP1, IGSF11, ZSCAN18, GFRA2, GFRA1, FGFR1, HDAC4, GHR, TIMP3, CLIP4, TAGLN and ILK) in different subtypes of GC. SATB1 shows positive correlation with GC promoting oncogenes and enhances the expression of metastasis associated genes in GC samples to potentiate GC progression. SATB1 enhances WNT/NOTCH signaling axis in GC samples. In contrast, STAB1 suppresses a network of tumor suppressor genes in GC samples. Moreover, SATB1 expression negatively correlates with GC patient’s survival. Importantly, we found that SATB1 co-amplified genes CLIP4, DZIP1 and PRICKLE2 independently involve in GC progression. Overexpression of CLIP4, DZIP1 and PRICKLE2 show poor survival rates in GC patients. DZIP1 empowers AKT3/FGF2/FGFR1 and CXCR3/CXCR6 in GC patients. PRICKLE2 enhances GC progression by targeting FGFR1/NOTCH3/WNT4 signaling. Taken together, these results identified novel roles of SATB1, CLIP4, DZIP1 and PRICKLE2 in the GC invasion, migration and metastasis using cancer bioinformatics approach. These results highlights clinical significance of SATB1, CLIP4, DZIP1 and PRICKLE2 in GC patients and furthermore, these proteins may serve as prognostic markers in GC. These results also provide SATB1, CLIP4, DZIP1 and PRICKLE2 as potential chemotherapeutic targets in treatment of gastric cancer patients.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47722977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Efect of sowing dates and seed rates of flax intercropped with sugar beet on productivity of both crops and competitive relationships 亚麻与甜菜间作播期和结实率对两种作物产量的影响及竞争关系

Romanian Biotechnological Letters Pub Date : 2021-12-30 DOI: 10.25083/rbl/26.6/3074-3089

A. Abd-Rabboh, Yaser Mazrou, Amal M. A. El-Borhamy, W. Abdelmasieh, Y. Hafez, K. Abdelaal

{"title":"Efect of sowing dates and seed rates of flax intercropped with sugar beet on productivity of both crops and competitive relationships","authors":"A. Abd-Rabboh, Yaser Mazrou, Amal M. A. El-Borhamy, W. Abdelmasieh, Y. Hafez, K. Abdelaal","doi":"10.25083/rbl/26.6/3074-3089","DOIUrl":"https://doi.org/10.25083/rbl/26.6/3074-3089","url":null,"abstract":"The aim of intercropping flax on sugar beet is to increase the cultivated area with flax due to widely cultivated area of sugar beet. So, a field experiment was carried out to study the effect of various sowing dates and seed rates of flax intercropped with sugar beet on yield, quality and economic evaluation. The main-plots were assigned to three sowing dates of flax intercropped with sugar beet and the sub-plots were allocated to four seed rates of flax. The results show that sowing date of flax after 35 days from sowing (DFS) sugar beet resulted in the highest values of sugar beet yields and quality. Maximum values of flax straw and seed yields resulted from sowing flax after 21 DFS sugar beet. Intercropping sugar beet with flax at 12.5% from recommended seed rate on top sugar beet terraces attained the most elevated values of sugar beet yield and quality. Sowing flax at 50% from recommended rate resulted in the best values of flax technical and fruiting zone length, also straw and seed yield/hectare. The maximum yield and economic return of both crops were obtained from sowing flax at 12.5% from recommended rate after (21 or 35) DFS sugar beet.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41999557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and phenotypic characterization of Gallibacterium anatis biovar haemolytica from a hen with hemorrhagic ooforitis 从一只出血性卵巢炎母鸡身上分离并鉴定溶血性锐金杆菌的表型特征

Romanian Biotechnological Letters Pub Date : 2021-12-30 DOI: 10.25083/rbl/26.6/3090-3094

I. Sorescu, L. Romaşcu, M. Ionescu, A. Popovici, Costin Stoica, F. Bărbuceanu

{"title":"Isolation and phenotypic characterization of Gallibacterium anatis biovar haemolytica from a hen with hemorrhagic ooforitis","authors":"I. Sorescu, L. Romaşcu, M. Ionescu, A. Popovici, Costin Stoica, F. Bărbuceanu","doi":"10.25083/rbl/26.6/3090-3094","DOIUrl":"https://doi.org/10.25083/rbl/26.6/3090-3094","url":null,"abstract":"Objective: the isolation and phenotypically identification of a Gallibacterium anatis biovar haemolytica strain from a hen with hemorrhagic ooforitis; the antimicrobial susceptibility testing of this isolate. Methods and results: a strain of G. anatis biovar haemolytica, was isolated and phenotypic identified by morphological, cultural and biochemical characters examination, with API 20 E, API 20 NE, API STAPH, API ZYM tests and ABIS online software. The antimicrobial susceptibility of the isolate was performed using the standard disk diffusion method. Conclusions: a strain of G. anatis biovar haemolytica was isolated and phenotypically identified from a hen. From our knowledge, this is the first reporting in Romania of isolation and identification of G. anatis biovar haemolytica. The Gah IDSA 161 strain could be phenotypic identified only by ABIS on line software, Pasteurellaceae Database version, unifying the results of four API kits and other biochemical tests. The isolate showed a multi-drug resistant profile to tetracyclines (tetracycline, oxitetracycline, doxicyclin), floroquinolones (enrofloxacin, ciprofloxacin), ampicillin, trimethoprim, nalidixic acid, clindamycin, and it was susceptible to sulfonamide, sulfomethoxazole/trimethoprim, gentamicin, cephalothin, streptomycin, amoxicilin/clavulanic acid.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46114221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evaluation of sowing methods and nitrogen levels for grain yield and components of durum wheat under arid region of Egypt 埃及干旱区硬粒小麦播种方式及氮素水平对籽粒产量及组成的影响

Romanian Biotechnological Letters Pub Date : 2021-12-30 DOI: 10.25083/rbl/26.6/3031-3039

Mohamed Mosalem, Yaser Mazrou, S. Badawy, Mohamed Abd EL-RAHMAN Abd Ullah, Mohamed Ghoneim Mubarak, Y. Hafez, K. Abdelaal

{"title":"Evaluation of sowing methods and nitrogen levels for grain yield and components of durum wheat under arid region of Egypt","authors":"Mohamed Mosalem, Yaser Mazrou, S. Badawy, Mohamed Abd EL-RAHMAN Abd Ullah, Mohamed Ghoneim Mubarak, Y. Hafez, K. Abdelaal","doi":"10.25083/rbl/26.6/3031-3039","DOIUrl":"https://doi.org/10.25083/rbl/26.6/3031-3039","url":null,"abstract":"The present experiment was conducted at private farm during 2018/19 and 2019/20 seasons. The investigated area lies within the extremely arid belt, having long hot summer and short warm winter. The main goal of this investigation was to evaluate the effect of different sowing methods on yield components of wheat, using split plot design with three replications. Sowing methods in main plot were broadcasting method, row spacing 10, 15 and 20 cm apart). Nitrogen fertilizer levels in sub plot were 60, 75 and 90 kg N fed-1 (fed = 4200 m-2). The increase percentage due to row spacing at 20 cm apart (M4) compared to broadcasting method (M1) were (23.30 & 26.74%) for spike length; (5.11 & 6.94 %) for 1000 grain weight and (8.02 & 7.74%) for grain yield and according ally (3.07 & 3.08%) for harvest index in both seasons, respectively. Overall, from the present study the sowing method in rows with 20 cm apart and the optimum N fertilizer rate (90 kg fed-1) for durum wheat production in the soils of Qasir, Dakhala oasis on silt loam soil was the best treatment.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42523223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Studying some neuroprotective effects of Calotropis procera extracts against scopolamine- induced neuropschiatric comorbidities in a rodent model of epilepsy 在啮齿动物癫痫病模型中，研究原心甘油三酯提取物对东莨菪碱诱导的神经精神合并症的神经保护作用

Romanian Biotechnological Letters Pub Date : 2021-12-30 DOI: 10.25083/rbl/26.6/3114-3119

Prosper T. Kinda, S. Guenné, Basile Tindano, N. Ouedraogo, Nabéré Ouattara, P. Zerbo, R. Duță, A. Ciobica, M. Kiendrebeogo

{"title":"Studying some neuroprotective effects of Calotropis procera extracts against scopolamine- induced neuropschiatric comorbidities in a rodent model of epilepsy","authors":"Prosper T. Kinda, S. Guenné, Basile Tindano, N. Ouedraogo, Nabéré Ouattara, P. Zerbo, R. Duță, A. Ciobica, M. Kiendrebeogo","doi":"10.25083/rbl/26.6/3114-3119","DOIUrl":"https://doi.org/10.25083/rbl/26.6/3114-3119","url":null,"abstract":"Many plants are largely used in alternative medicine of Burkina Faso for neuropsychiatric disorders treatment. However, their neuro-pharmacological properties are less evaluated through scientific studies. The present study aims to evaluate the neuroprotective effect of Calotropis procera leaves and root-bark aqueous extract, focusing on a scopolamine-induced model of epilepsy in rodents. In this study, we evaluated this plant extracts possible protective effects on the central nervous system, through the behavioral tests and the enzymes activity assays. Thus, elevated plus-maze test and Y-maze task were used to evaluate animals behavioral and UV/visible spectrophotometer methods were used to evaluate the enzyme’s activities in brain’s supernatant. Our results are showing no significant protective effects of leaves extract, but it revealed a significant neuroprotective effect of root-bark aqueous extract, as well as in the behavioral tests and the brain’s oxidative enzymes specific activity evaluation. Indeed, anti-amnesic and anxiolytic activities were observed through Y maze task and elevated plus maze tests for the groups of animals receiving root-bark extract (100 mg/kg b.w.). In these test, inhibition of disturbances of Time spent in Open Arms, Spontaneous Alternation, and Transfer Latency induced after scopolamine administration were recorded with animals received root-bark extract. Likewise, the superoxide dismutase and catalase activity disturbance induced by scopolamine were also inhibited in root-bark extract pre-administered group. Thus, our study provides biochemical and neuro-pharmacological data for traditional use of C. procera for neuropsychiatric disorders treatment, including scopolamine-induced epilepsy symptoms (mainly referring to the psychiatric comorbidities of this disorder).","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41389661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Yield results obtained in maize crop following the foliar fertilization with new biostimulators based on keratin and collagen hydrolysates of animal origin 以动物源性角蛋白和胶原蛋白水解物为基础的新型生物刺激剂对玉米叶片施肥后的产量结果

Romanian Biotechnological Letters Pub Date : 2021-12-30 DOI: 10.25083/rbl/26.6/3129-3136

Marius Becheritu, R. Horoias, C. Cioineag, Paul Borovină, C. Gaidău, M. Niculescu

{"title":"Yield results obtained in maize crop following the foliar fertilization with new biostimulators based on keratin and collagen hydrolysates of animal origin","authors":"Marius Becheritu, R. Horoias, C. Cioineag, Paul Borovină, C. Gaidău, M. Niculescu","doi":"10.25083/rbl/26.6/3129-3136","DOIUrl":"https://doi.org/10.25083/rbl/26.6/3129-3136","url":null,"abstract":"The aim of the paper is to show the reaction of maize crop to the foliar application of new biofertilizers, obtained from the residues left after the wool tanning process and which are based on protein hydrolysates of keratin and collagen. The tests have been performed in microplots (10 sqm) and macroplots (1000 sqm), in three repetitions, in southern Romania. All data obtained as a result of the 2020 autumn harvest have been centralized in tables and statistically processed, in order to establish the influence of each product, compared to the control. The bifactorial analyses show that, in climatic stress conditions (prolonged drought – Calarasi), very significant increases in yield have been obtained with K4 (2.5 and 5.0 l/ha), K5 (5.0 l/ha) and C (5.0 l/ha), with increases between 700 and 1300 kg/ha, ie up to +24%. In macroplots, subject to a moderate drought (Teleorman), product C (5.0 l/ha) brought a yield increase of 1537 kg/ha (+26%), noting that in this location almost all tested biostimulators offer very significant yield growth (except KC). Based on the 2020 yields, it was possible to identify which the foliar biostimulators that will continue to be tested in 2021.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42876073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental justification for prompt neutralization of traumatic action of thermal exo- and endogenous damage factors (morphological features) 热外源性和内源性损伤因子迅速中和创伤作用的实验证明(形态学特征)

Romanian Biotechnological Letters Pub Date : 2021-12-30 DOI: 10.25083/rbl/26.6/3040-3046

V. Nagaichuk, R. Chornopyshchuk, O. Nazarchuk, Ludmila Sidorenko, M. Zheliba, O. Urvan

引用次数: 0

Researches on the Epilobium angustifolium L. ethanol extract, Turkey 土耳其枇杷乙醇提取物的研究

Romanian Biotechnological Letters Pub Date : 2021-09-20 DOI: 10.25083/rbl/26.5/2964.2970

H. Ç. Onar, T. Yıldız

{"title":"Researches on the Epilobium angustifolium L. ethanol extract, Turkey","authors":"H. Ç. Onar, T. Yıldız","doi":"10.25083/rbl/26.5/2964.2970","DOIUrl":"https://doi.org/10.25083/rbl/26.5/2964.2970","url":null,"abstract":"In this paper, Epilobium angustifolium L. in Turkey was investigated for total phenolics, flavonoids, and anthocyanin amounts. Furthermore, the ethanol extract of the leafs was evaluated for antioxidant activities as ABTS, hydroxyl radical scavenging, superoxide radical scavenging, power of reduction, and bleaching of beta-carotene. The total phenolic and flavonoid contents of the ethanolic extract were found to be 468,11± 1,53 micrograms of pyrocatechol per milligram of the extract and 103,05 ± 2,36 µg catechin per milligram of the extract, respectively. As the extract concentration increased, the amount of anthocyanins showed a decrease (17.92±8.08 and 13.56±8.22 for the 1 mg/mL and 2 mg/mL concentrations, respectively). In the β-carotene bleaching test, higher activity was observed than BHA ( 1.540±0.053 and 1, respectively). The results of the reduction power, hydroxyl radical scavenging, and ABTS tests gave either very close values or higher than the standards chosen. Superoxide radical scavenging activity gave a lower value than the Trolox selected as standard. Antioxidant activity of ethanol extract was found to be 94.22±0.05958 (ABTS), 78.77±0.84099 (hydroxyl radical scavenging) and 47.86±0.03915 (superoxide radical scavenging). The experimental data indicate that Turkey Epilobium angustifolium L. ethanol extract can be used pharmacologically.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43240655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mapping QTLs controlling the biosynthesis of maltose in soybean 控制大豆麦芽糖生物合成的QTL定位

Romanian Biotechnological Letters Pub Date : 2021-09-20 DOI: 10.25083/rbl/26.5/2936.2941

P. Jha, Vineet Kumar, A. Rani, Anil Kumar

引用次数: 1

Purification and characterization of thermo-alkaline stable lipase from Bacillus coagulans and its compatibility with commercially available detergents 凝固芽孢杆菌热碱性稳定脂肪酶的纯化和特性及其与市售洗涤剂的相容性

Romanian Biotechnological Letters Pub Date : 2021-09-20 DOI: 10.25083/rbl/26.5/2994.3001

Abdullah A. Al-Ghanayem

{"title":"Purification and characterization of thermo-alkaline stable lipase from Bacillus coagulans and its compatibility with commercially available detergents","authors":"Abdullah A. Al-Ghanayem","doi":"10.25083/rbl/26.5/2994.3001","DOIUrl":"https://doi.org/10.25083/rbl/26.5/2994.3001","url":null,"abstract":"Thermo-alkaline stable lipase producing B. coagulans was isolated and identified by 16S rRNA sequencing. The lipase production was optimized using different growth parameters. The enzyme was purified and characterized in terms of pH, temperature, solvents, heavy metal ions and inhibitors. Compatibility with commercially available detergents was also studied. The isolate showed maximum lipase production at 37 ⁰C; pH of 9 within 48 h. Addition of magnesium chloride increased lipase production. Sephadex G-100 chromatography was used to purify lipase. The enzyme showed maximum activity at pH 8 of 30 ⁰C. Lipase form B. coagulans was active at a wide range of temperature between 30-70 ⁰C. It was stable in most of the solvents at a concentration 5 and 10%, except dimethyl sulfoxide (DMSO) and methanol. Iodoacetic acid (IAA) and p-chloromercuribenzoic acid (pCMB) had an inhibitory effect on lipase. The lipase was compatible with commercially available detergents that increased the brightness and whiteness of the tested cotton fabrics. Lipase from B. coagulans with alkaline stability at a wide range of temperature has potential application in the detergent industry.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43794884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0