Feasibility study of fluorescence quantitative PCR for the detection of microecological dynamics in fermented maize stover feeds

Abstract

During the fermentation of corn stalk bio-feed, the quantity of bacteria in corn stalk bio-feed was counted by fluorescence quantitative PCR and plate colony counting respectively. The comparative analysis of these studies was used to explore the feasibility of fluorescence quantification methods and changes in microbiota during fermentation. The results showed that the standard deviation of fluorescence quantitative method was smaller than that of plate method, but the trend was similar. The biomass of Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae reached their maximum on the third, fifth and fifth day respectively, and then decreased gradually and maintained at a certain level. The experiment showed that the fluorescence quantitative PCR method can accurately quantify the number of bacteria in corn stalk bio-feed, and it is a better method to quantitatively detect the dynamic changes of different kinds of bacteria in corn stalk bio-feed.