Romanian Biotechnological Letters最新文献_第4页

Histology of maize seeds and young germinating embryos after liquid nitrogen exposure 暴露于液氮后玉米种子和幼胚的组织学

Romanian Biotechnological Letters Pub Date : 2021-06-29 DOI: 10.25083/rbl/26.4/2855-2861

A. Villalobos-Olivera, R. Pereira, Daviel Gómez, Julia Martínez, D. Escalante, Marcos Edel Martínez-Montero, E. Hajari, L. Yabor, Ó. Vicente, José Carlos Lorenzo

{"title":"Histology of maize seeds and young germinating embryos after liquid nitrogen exposure","authors":"A. Villalobos-Olivera, R. Pereira, Daviel Gómez, Julia Martínez, D. Escalante, Marcos Edel Martínez-Montero, E. Hajari, L. Yabor, Ó. Vicente, José Carlos Lorenzo","doi":"10.25083/rbl/26.4/2855-2861","DOIUrl":"https://doi.org/10.25083/rbl/26.4/2855-2861","url":null,"abstract":"Maize represents a staple food crop and is the second most important agricultural commodity globally. Considering the important role of maize for food security, the long-term conservation of valuable germplasm is critical to ensure that high levels of genetic diversity are available for breeding superior cultivars to face future challenges. Cryopreservation is regarded as the most appropriate tool for long-term germplasm preservation and has been investigated in different crops. This short communication adds to the existing knowledge on maize cryopreservation by describing histological changes observed in maize seeds and young germinating embryos after liquid nitrogen (LN) exposure. Plants were examined immediately after recovery from LN (day zero) and following 3 days of germination. At day 3, seeds exposed to LN showed lower germination rates than non-cryostored seeds, i.e., 60.7% vs. 83.3%. Histological evaluation at day 3 revealed that the thickness of the conical endosperm and the scutellum did not show any statistically significant differences between control and cryopreserved seeds. In contrast, for the other histological evaluations made, mostly regarding the thickness of mesocarp, mealy endosperm, plumule, radicle and the epidermis, significant differences were observed between control and cryostored seeds with the former consistently displaying higher average values than the latter.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47039758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative computational analysis of SARS-CoV- 2 nucleocapsid epitope with taxonomically related corona viruses 严重急性呼吸系统综合征冠状病毒2型核衣壳表位与分类相关冠状病毒的比较计算分析

Romanian Biotechnological Letters Pub Date : 2021-06-29 DOI: 10.25083/RBL/26.4/2737.2750

S. Elkholy

{"title":"Comparative computational analysis of SARS-CoV- 2 nucleocapsid epitope with taxonomically related corona viruses","authors":"S. Elkholy","doi":"10.25083/RBL/26.4/2737.2750","DOIUrl":"https://doi.org/10.25083/RBL/26.4/2737.2750","url":null,"abstract":"Several research lines are currently ongoing to address the multitude of facets of the pandemic COVID-19. In line with the One-Health concept, extending the target of the studies to the animals which humans are continuously interacting with may favor a better understanding of the SARS-CoV-2 Biology and pathogenetic mechanisms;thus, helping to adopt the most suitable containment measures. The last two decades have already faced severe manifestations of the coronavirus infection in both humans and animals, thus, circulating epitopes from previous outbreaks might confer partial protection from SARS-CoV-2 infections. In the present study, we provide computational analysis of the major nucleocapsid protein epitopes and compare them with the homologues of taxonomically-related coronaviruses with tropism for animal species that are closely inter-related with the human being population all over the world. Protein sequence alignment provides evidence of high sequence homology for some of the investigated proteins. Moreover, the way the receptor binding domains of the nucleocapsid epitopes interact with their specific proteins is different from the closely related viruses. These evidences provide a molecular structural rationale for a potential role in conferring protection from SARS-CoV-2 infection and identifying potential candidates for the development of diagnostic tools and prophylactic- oriented strategies.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":"26 1","pages":"2737-2750"},"PeriodicalIF":0.0,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48053465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytotoxicity evaluation and physicochemical properties of collagen isolated from silver carp tails as potential biomaterial 鲢鱼尾胶原蛋白潜在生物材料的细胞毒性评价及理化性质

Romanian Biotechnological Letters Pub Date : 2021-06-29 DOI: 10.25083/rbl/26.4/2808-2817

P. Preda, A. Enciu, M. Avram, C. Tanase, M. Dudău, I. Mihalache, O. Tutunaru, D. Stan, A. Ficai

引用次数: 2

Evaluation of the antiviral and immunomodulatory activity of nutritional supplement formula in a randomized, double-blind, placebo-controlled clinical trial 在一项随机、双盲、安慰剂对照的临床试验中评估营养补充剂配方的抗病毒和免疫调节活性

Romanian Biotechnological Letters Pub Date : 2021-06-29 DOI: 10.25083/rbl/26.4/2869-2878

Bogdan Soltuzu

{"title":"Evaluation of the antiviral and immunomodulatory activity of nutritional supplement formula in a randomized, double-blind, placebo-controlled clinical trial","authors":"Bogdan Soltuzu","doi":"10.25083/rbl/26.4/2869-2878","DOIUrl":"https://doi.org/10.25083/rbl/26.4/2869-2878","url":null,"abstract":"Introduction: Sea buckthorn and bee products have a millennial tradition in oriental medicine, being used by Mongols, Tibetans, or Chinese both for the prevention and treatment of colds and increase the body immunity. Objective: To evaluate the antiviral effect of a nutritional supplement formula based on sea buckthorn, honey, and propolis to reduce the incidence of respiratory viruses and the severity of their symptoms. Simultaneously, the aim was to evaluate this supplement formula’s immunomodulatory activity on blood cell count (CBC) and on immunoglobulin A (IgA) level. Subjects: A total of 50 volunteers, of whom 25 received a nutritional supplement formula based on sea buckthorn, honey, and propolis product at random, and 25 received a placebo product. The duration of the product administration was 30 days. Results: The number of viral infections decreased statistically significantly (p < 0.05) in the group receiving the nutritional supplement formula based on sea buckthorn, honey, and propolis product SBHPG compared to the placebo group PG, reducing the relative risk of infection by 71.42%. The immunomodulatory effect in SBHPG consisted of a statistically significant reduction in the number of neutrophils and basophils and an increase in the number of lymphocytes than PG (p < 0.05). Conclusion: The nutritional supplement formula decreased respiratory viruses infections incidence and reduced the severity of the symptoms.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42344235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Ruminal Acidosis’s effect over the Milk production in dairy Cows 奶牛瘤胃酸中毒对产奶量的影响

Romanian Biotechnological Letters Pub Date : 2021-06-29 DOI: 10.25083/rbl/26.4/2788-2792

V. Boghian

引用次数: 0

Effect of ethanolic extract of Silybum marianum L. Gaertn. on lipid peroxidation inhibition and microbial count in minced beef 水飞蓟乙醇提取物的作用。牛肉糜中脂质过氧化抑制作用及微生物计数的研究

Romanian Biotechnological Letters Pub Date : 2021-06-29 DOI: 10.25083/rbl/26.4/2773-2778

W. Fahmy, S. Abdelhady, Samir Mahmoud Metwaly, F. Ahmed

{"title":"Effect of ethanolic extract of Silybum marianum L. Gaertn. on lipid peroxidation inhibition and microbial count in minced beef","authors":"W. Fahmy, S. Abdelhady, Samir Mahmoud Metwaly, F. Ahmed","doi":"10.25083/rbl/26.4/2773-2778","DOIUrl":"https://doi.org/10.25083/rbl/26.4/2773-2778","url":null,"abstract":"The present research was carried out for two main targets, the first one is phytochemical and biological screening of the aerial parts of Silybum marianum. The second one is the possibility of using Silybum marianum ethanolic extract as natural preservative for extending minced beef shelf-life during refrigeration and frozen storage. The chemical analysis of dry matter revealed that total ash content was 13.04% of D.M, crude fibers, total phenolic compounds, total flavonoids and total tannins were (24.59%, 9.16%, 0.84% and (0.08%), respectively. The highest values of antioxidant activity were recorded in ethyl acetate and ethyl alcohol 70%extacts, respectively for successive and selective solvents with values of 82.45% and 58.85% for successive solvents, 86.84% and 70.50% for selective solvents, respectively. Antimicrobial activity showed that, S. marianum L. Gaertn. had effect on gram positive, gram negative bacteria and yeast but had no effect on different strains of studied fungi. S. marianum had an influence on microorganisms growth in minced beef, the highest effect was 1.0% for 60 days (56 CFU). The most effective concentrations on lipid peroxidation prevention in minced beef were 1.5% for 3 days and 1.5 for 60 days.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41775797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Computational Analysis of SARSCoV-2 Nucleocapsid Epitope with Taxonomically Related Corona Viruses SARSCoV-2核衣壳表位与相关冠状病毒的比较计算分析

Romanian Biotechnological Letters Pub Date : 2021-06-29 DOI: 10.25083/rbl/26.4/2737-2750

S. Elkholy

{"title":"Comparative Computational Analysis of SARSCoV-2 Nucleocapsid Epitope with Taxonomically Related Corona Viruses","authors":"S. Elkholy","doi":"10.25083/rbl/26.4/2737-2750","DOIUrl":"https://doi.org/10.25083/rbl/26.4/2737-2750","url":null,"abstract":"Several research lines are currently ongoing to address the multitude of facets of the pandemic COVID-19. In line with the One-Health concept, extending the target of the studies to the animals which humans are continuously interacting with may favor a better understanding of the SARS-CoV-2 Biology and pathogenetic mechanisms; thus, helping to adopt the most suitable containment measures. The last two decades have already faced severe manifestations of the coronavirus infection in both humans and animals, thus, circulating epitopes from previous outbreaks might confer partial protection from SARS-CoV-2 infections. In the present study, we provide computational analysis of the major nucleocapsid protein epitopes and compare them with the homologues of taxonomically-related coronaviruses with tropism for animal species that are closely inter-related with the human being population all over the world. Protein sequence alignment provides evidence of high sequence homology for some of the investigated proteins. Moreover, the way the receptor binding domains of the nucleocapsid epitopes interact with their specific proteins is different from the closely related viruses. These evidences provide a molecular structural rationale for a potential role in conferring protection from SARS-CoV-2 infection and identifying potential candidates for the development of diagnostic tools and prophylactic- oriented strategies.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41585954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Balady bread: A comprehensive study on sensory, chemical and rheological properties during fortification by Quinoa (Chenopodium quinoa Willd.) 巴拉迪面包:藜麦强化过程中感官、化学和流变特性的综合研究

Romanian Biotechnological Letters Pub Date : 2021-06-29 DOI: 10.25083/rbl/26.4/2846-2854

A. F. Nahla, W. Badawy, El-Bana, H. Kassab

引用次数: 0

Amylase Production by Aspergillus niger immobilized in Microporous Calcium Alginate Gel Beads 微孔海藻酸钙凝胶珠固定化黑曲霉生产淀粉酶的研究

Romanian Biotechnological Letters Pub Date : 2021-06-29 DOI: 10.25083/rbl/26.4/2825-2833

U. Oyeagu, C. Nwuche, C. Ogbonna, J. Ogbonna

{"title":"Amylase Production by Aspergillus niger immobilized in Microporous Calcium Alginate Gel Beads","authors":"U. Oyeagu, C. Nwuche, C. Ogbonna, J. Ogbonna","doi":"10.25083/rbl/26.4/2825-2833","DOIUrl":"https://doi.org/10.25083/rbl/26.4/2825-2833","url":null,"abstract":"Microporous calcium alginate gel beads were investigated as potential solutions to mass transfer limitations in immobilized cultures. The beads were prepared by gelling mixtures of sodium alginate and fillers (starch or granulated sugars) in calcium chloride solution. The resulting beads were cured in the same solution, during which the fillers leached out of the beads thereby creating micro-pores in the beads (microporous beads). The effectiveness of the microporous beads in improving amylase production by Aspergillus niger LC 269109 was investigated. Spores of A. niger were immobilized in the microporous beads and used for batch alpha amylase and gluco amylase production. Amylase production by the A. niger immobilized in the microporous beads were significantly higher (p<0.01) than the values obtained with the conventional calcium alginate gel beads. Under all the conditions investigated, gluco-amylase activities were significantly (p<0.01) higher than the alpha-amylase activities. Under the optimum conditions of inoculum concentration (1.0 × 105 spores/ml), pH (6), temperature (35°C), bead diameter (3 mm) and calcium chloride concentration (1.5%), the gluco-amylase and alpha amylase activities were 11.98 U/ml and 6.7 U/ml respectively, which were significantly higher (p<0.05) than the 7.8 U/ml and 3.2 U/ml obtained with the conventional gel beads.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69221046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitrogen Fixation and Diazotrophs – A Review 固氮和重氮营养体研究进展

Romanian Biotechnological Letters Pub Date : 2021-06-29 DOI: 10.25083/rbl/26.4/2834-2845

Wenli Sun, M. H. Shahrajabian, Qi Cheng

{"title":"Nitrogen Fixation and Diazotrophs – A Review","authors":"Wenli Sun, M. H. Shahrajabian, Qi Cheng","doi":"10.25083/rbl/26.4/2834-2845","DOIUrl":"https://doi.org/10.25083/rbl/26.4/2834-2845","url":null,"abstract":"Nitrogen fixation involves formation of ammonium from N2, which needs a high input of energy. Biological nitrogen fixation utilizes the enzyme nitrogenase and ATP to fix nitrogen. Nitrogenase contains a Fe-protein and a Mo-Fe-protein and other metal cofactors. Soil diazotrophs possess the function of fixing atmospheric N2 into biologically available ammonium in ecosystems. In Aechaea, nitrogen fixation has been reported in some methanogens such as Methanobacteriales, Methanococcales, and Methanosarcinales. Community structure and diversity of diazotrophic are correlated with soil pH. All known organisms which involve in nitrogen-fixing which are called diazatrophs are prokaryotes, and both bacterial and archaeal domains are responsible for that. Diazotrophs are categorized into two main groups namely: root-nodule bacteria and plant growth-promoting rhizobacteria. Diazotrophs include free living bacteria, such as Azospirillum, Cupriavidus, and some sulfate reducing bacteria, and symbiotic diazotrophs such Rhizobium and Frankia. Two important parameters which may affect diazotroph communities are temperature and soil moisture in different seasons. To have sustainable agriculture, replacing expensive chemical nitrogen fertilizers with environmentally friendly ways is the most accepted practice.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43408872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14