Romanian Biotechnological Letters最新文献_第5页

Potential of wild oregano essential oil in control of Monilinia rot on stored sweet cherry fruits 野生牛至精油防治甜樱桃果实霉菌病的潜力

Romanian Biotechnological Letters Pub Date : 2021-06-29 DOI: 10.25083/rbl/26.4/2779-2787

J. Kalajdžić, B. Milić, A. Stankov, M. Petreš, M. Grahovac, Jelena Vukotić, V. Stojsin

引用次数: 2

Manipulation of sunflower population density and herbicide rate for economical and sustainable weed management 向日葵种群密度和除草剂用量的调控对杂草的经济可持续管理

Romanian Biotechnological Letters Pub Date : 2021-06-29 DOI: 10.25083/rbl/26.4/2751-2758

E. Štefanić, Slavica Antunović, B. Japundžić-Palenkić, D. Zima

引用次数: 1

Improving the net present value by estrus synchronization in non-pregnant dairy cows detected by ultrasound 25 days after artificial insemination 人工授精后25天超声检测未怀孕奶牛发情同步提高净现值

Romanian Biotechnological Letters Pub Date : 2021-06-29 DOI: 10.25083/rbl/26.4/2818-2824

S. Borş, Ș. Creangă, A. Borș

{"title":"Improving the net present value by estrus synchronization in non-pregnant dairy cows detected by ultrasound 25 days after artificial insemination","authors":"S. Borş, Ș. Creangă, A. Borș","doi":"10.25083/rbl/26.4/2818-2824","DOIUrl":"https://doi.org/10.25083/rbl/26.4/2818-2824","url":null,"abstract":"Pregnancy rates in Holstein cow showed a substantial decline in the past years, which caused intensive estrus synchronization programs for non-pregnant cows to shorten the period between an unsuccessful artificial insemination (AI) and the next attempt on the same cow. The program implied estrus synchronization of non-pregnant cows with either prostaglandin or GnRH followed by prostaglandin after 7 days, then GnRH 2 days later and AI during the following day. Cows included in the estrus synchronization program recorded an improvement of the pregnancy rate at 130 days in milk compared to those in which this strategy was not applied (56.7% vs. 37.5%; P<0.05). The economic analysis revealed a difference in the net present value (NPV; $/cow/year) of $10.1/cow/year between the two programs (rebreeding the non-pregnant cows following estrus synchronization program and AI at detected estrus vs. AI at detected estrus). In summary, rebreeding the non-pregnant dairy cows after early negative pregnancy diagnosis (25 days after AI) using estrus synchronization program can improve the pregnancy rate and economic value of dairy farms.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46701717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of fat replacement with modified starch on composition and quality of cream cheese 变性淀粉替代脂肪对奶油奶酪成分和品质的影响

Romanian Biotechnological Letters Pub Date : 2021-06-29 DOI: 10.25083/rbl/26.4/2793-2799

N. Mehanna, A. Mahmoud, A. Elshenawy, R. EL-AHWAL

引用次数: 1

Monitoring of Aflatoxin B1 in Dry Aged Meats using a modified HPLC-FLD method 改良HPLC-FLD法监测干陈肉中黄曲霉毒素B1

Romanian Biotechnological Letters Pub Date : 2021-06-29 DOI: 10.25083/rbl/26.4/2885-2891

H. Kahraman, H. Tutun

{"title":"Monitoring of Aflatoxin B1 in Dry Aged Meats using a modified HPLC-FLD method","authors":"H. Kahraman, H. Tutun","doi":"10.25083/rbl/26.4/2885-2891","DOIUrl":"https://doi.org/10.25083/rbl/26.4/2885-2891","url":null,"abstract":"The presence of aflatoxins residues in meat and meat products occurs through ingestion of feeds contaminated with Aflatoxin B1 (AFB1) or during the processing of the meat products. Little research has been conducted concerning the detection of AFB1 on meat. The aim of this study was to modify the method for AFB1 determination by high performance liquid chromatography with fluorescence detection in dry aged meats. The aged meat samples used for determination of AFB1 were obtained from a commercial dry aged meat plants and produced for 28 days in a meat plant chiller. All meat samples were analyzed according to the modified method. The samples were cleaned to remove impurities by using a immunoaffinity column. Photochemical reactor was used for the recording and evaluation of the chromatograms. The analytical performance parameters showed that the method was specific for AFB1 detection in the meat samples. Recovery rate, reproducibility (RSDr%) and repeatability (RSDR%) were found to be 85.889%, 3.822% and 6.601%, respectively. Limit of detection and limit of quantification were 0.15 ng/mL and 0.501 ng/mL, respectively. AFB1 was not detected in the meat samples. Our results indicated that the method modified for monitoring AFB1 level in the meats was simple, time-saving and reliable.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49349691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Entomopathogenic Fungi on Fabae bean Aphid, Aphis craccivora (Koch) (Hemiptera: Aphididae) 蚕豆蚜、卡氏蚜的昆虫病原真菌（半翅目：蚜科）

Romanian Biotechnological Letters Pub Date : 2021-06-29 DOI: 10.25083/rbl/26.4/2862-2868

Abdel-Raheem, Abla F. A. Saad, I. Abdel-Rahman

{"title":"Entomopathogenic Fungi on Fabae bean Aphid, Aphis craccivora (Koch) (Hemiptera: Aphididae)","authors":"Abdel-Raheem, Abla F. A. Saad, I. Abdel-Rahman","doi":"10.25083/rbl/26.4/2862-2868","DOIUrl":"https://doi.org/10.25083/rbl/26.4/2862-2868","url":null,"abstract":"The Aphids, Aphis craccivora (Koch) is a serious pest in agricultural fields in Egypt. Entomopathogenic fungi are biological control agents of insects. The study was carried out on the aphid by using of Bio Catch, Verticillium lecanii, Bio Power, Beauveria bassiana and Bio Magic, Metarhizium anisopliae on A. craccivora. Laboratory experiments were done to measure the pathogenicity of three commercial compounds from entomopathogenic fungi, Bio Catch, V. lecanii, Bio Power, B. bassiana, and Bio Magic, M. anisopliae against adults and nymphs of A. craccivora. Three concentrations were used from all compounds, 107 , 108 and 109 spores/ml. at 22 ± 2°C and 75 ± 5 R.H. The concentration (109 spores/ml.) gave 100% mortality with V. lecanii and B. bassiana then M. anisopliae. The Lowest LC50 value of 2.1 x 106 spores/ml. was recorded by V. lecanii, which showed higher virulence compared to other entomopathogenic fungi. The LC50 values of V. lecanii, B. bassiana and M. anisopliae were 2.1 x 106 , 4.3 x 106 and 6.4 x 107 spores ml., respectively. At the highest concentration of 109 spores/ml., the Median LT50 values for V. lecanii, B. bassiana and M. anisopliae were 4.2, 5.2 and 7.0 days, respectively.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48759079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Maternal serum biochemical markers combined with ultrasound diagnosis at the end of the first trimester 孕早期末母体血清生化指标结合超声诊断

Romanian Biotechnological Letters Pub Date : 2021-06-29 DOI: 10.25083/rbl/26.4/2879-2884

M. Marinaș, L. Zorilă, C. Patru, T. Ț. Cojan, V. Băleanu, D. Davițoiu, Bogdan Niculescu, A. Pascal, B. Socea, Efrem Ion Cristian, R. Căpitănescu

引用次数: 1

Corn silage as a source of aflatoxin B1 in feed for dairy cattle 玉米青贮对奶牛饲料中黄曲霉毒素B1的影响

Romanian Biotechnological Letters Pub Date : 2021-06-29 DOI: 10.25083/rbl/26.4/2759-2764

D. Glamočić, M. P. Horvatović, I. Jajić, S. Krstović, M. Ivković, Noemi Čuzdi

{"title":"Corn silage as a source of aflatoxin B1 in feed for dairy cattle","authors":"D. Glamočić, M. P. Horvatović, I. Jajić, S. Krstović, M. Ivković, Noemi Čuzdi","doi":"10.25083/rbl/26.4/2759-2764","DOIUrl":"https://doi.org/10.25083/rbl/26.4/2759-2764","url":null,"abstract":"Nutrition of dairy cattle is based on two components, concentrates and forages. The main forages in Vojvodina, north province of Serbia is silage made from the whole plant of corn. After the outbreak of aflatoxin B1 in corn in 2012, the occurrence of aflatoxin B1 in corn as a source of contamination of aflatoxin M1 in milk was very broadly investigated. There is no data regarding the occurrence of aflatoxin B1 in silage and how much silage can contribute to the overall intake of aflatoxin B1 in this region. This work is an attempt to estimate how much silage, in condition and practice used in Vojvodina, contributes to the intake of aflatoxin B1, and consequently aflatoxin M1 in milk. In total, 82 samples of corn grain and 72 samples of corn silage were analyzed on the occurrence of aflatoxin B1 during 2017-2018 period. Aflatoxin B1 was found in 13.41% of corn samples in the range from 6.82 to 187.5 ppb (average 63.5 ppb). All positive samples were from 2017, while no positive samples were found during 2018. Incidence of aflatoxin B1 in silage was 54.17% in the range of 3.5-58.0 ppb (12% moisture content) or 0.95-16.1 ppb in the fresh matter. Results suggest that silage can be a significant factor to overall intake of aflatoxin B1 and that further research is needed.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48366852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, identification, preservation and determinations of lactic acid bacteria from chicken’s gut content at 26 days old 26日龄鸡肠道内容物中乳酸菌的分离、鉴定、保存和测定

Romanian Biotechnological Letters Pub Date : 2021-06-29 DOI: 10.25083/rbl/26.4/2765-2772

M. Dumitru, I. Sorescu, G. Ciurescu, M. Hăbeanu

{"title":"Isolation, identification, preservation and determinations of lactic acid bacteria from chicken’s gut content at 26 days old","authors":"M. Dumitru, I. Sorescu, G. Ciurescu, M. Hăbeanu","doi":"10.25083/rbl/26.4/2765-2772","DOIUrl":"https://doi.org/10.25083/rbl/26.4/2765-2772","url":null,"abstract":"Chichens’s gastrointestinal (GI) tract presents a large bacterial diversity and can be a beneficial source in order to the isolation of probiotics candidates. In this study, twentythree strains from Lactobacillus genus (one strain of L. acidophilus biotype 1, eight strains of L. acidophilus biotype 3, one of L. crispatus, five of L. fermentum biotype 1, two of L. brevis biotype 2, five of L. salivarius and one strain of L. delbrueckii subsp. delbrueckii) were isolated, cultured, and conserved from ileum and cecum digesta of sixteen chickens, 26 days old, based on their phenotypic characters. The strains confirmation was done by apiwebTM API 50 CHL V.5.1, BioMerieux (France) software, and ABIS online soft. The Lactobacillus strains (106 -108 CFU/g intestinal content) level in the selective medium were determined. The growth of Lactobacillus strains conserved at 4°C (from 48 days to 7 months) and room temperature (from 48 to 90 days) was evaluated. This study showed that chickens can be a stable source for isolating possibly active probiotic lactic acid bacteria (LAB). From Lactobacillus strains isolated and identified, respectively L. fermentum biotype 1 and L. brevis biotype 2 are theoretically suitable for continual testing of probiotic properties.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46044214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Green synthesis and characterization of silver nanoparticles using Indigofera aspalathoides leaf extract and study of its Antibacterial, Antioxidant, and Anti-cancerous activities 阿片靛蓝叶提取物绿色合成纳米银及其抗菌、抗氧化、抗癌活性研究

Romanian Biotechnological Letters Pub Date : 2021-04-11 DOI: 10.25083/RBL/26.3/2606-2617

V. Gandhiraj, K. Sathish

{"title":"Green synthesis and characterization of silver nanoparticles using Indigofera aspalathoides leaf extract and study of its Antibacterial, Antioxidant, and Anti-cancerous activities","authors":"V. Gandhiraj, K. Sathish","doi":"10.25083/RBL/26.3/2606-2617","DOIUrl":"https://doi.org/10.25083/RBL/26.3/2606-2617","url":null,"abstract":"Indigofera aspalathoides is an indigenous medicinal plant with copious phytochemical and pharmacological benefits. The crude extract from the leaves of Indigofera apalathoides aids in the bioreduction of silver nitrate into silver ions which results in the formation of colloidal silver nanoparticles. Critical physio-chemical parameters such as temperature, pH, substrate ratio (ratio of leaf extract and silver nitrate solution) and incubation time were optimized to increase the yield and efficiency of the nanoparticle formation. Synthesized nanoparticles were then characterized by performing a set of spectral analyses such as UV, FTIR, XRD, SEM, EDAX and AFM, later its bioactivity was also assessed. Characteristic UV-Vis absorption peak (λmax) at the range of 360-390 nm indicated silver nanoparticle formation and XRD pattern revealed the crystalline nature, while the average grain size was estimated to be 68.5 nm using Scherrer’s formula. SEM image clearly depicted the square shaped silver nanoparticles, which were synthesized using Indigofera aspalathoides leaf extract. Confirmation of formation of silver nanoparticles were carried out using EDAX spectrum. IC 50 value of free radical scavenging occurred at a lower concentration of 300 µg/ml, while a maximum inhibition zone of 13 mm was recorded against pathogenic bacterial strains and 6.7 mm was recorded against pathogenic fungal strains. Further, the silver nanoparticle cytotoxicity analysis was also carried out using MCF 7 cell line and IC 50 was found out to be 5.9 µg/ml. Silver nanoparticles which are green synthesized using the aqueous extract of Indigofera aspalathoides proved as an effective broad-spectrum antibiotic as well as an efficient antioxidant and an anti-cancerous agent.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":"26 1","pages":"2606-2617"},"PeriodicalIF":0.0,"publicationDate":"2021-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48890323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0