改良HPLC-FLD法监测干陈肉中黄曲霉毒素B1

H. Kahraman, H. Tutun
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引用次数: 0

摘要

肉和肉制品中黄曲霉毒素残留的存在是通过摄入被黄曲霉毒素B1(AFB1)污染的饲料或在肉制品加工过程中发生的。关于在肉上检测AFB1的研究很少。本研究的目的是改进高效液相色谱-荧光检测法测定干熟肉中AFB1的方法。用于测定AFB1的老化肉类样品从商业干燥老化肉类工厂获得,并在肉类工厂冷却器中生产28天。所有肉类样品均按照改进的方法进行分析。通过使用免疫亲和柱对样品进行清洁以去除杂质。光化学反应器用于记录和评估色谱图。分析性能参数表明,该方法对肉类样品中AFB1的检测具有特异性。回收率、重现性(RSDr%)和重复性(RSDr%)分别为85.889%、3.822%和6.601%。检测限和定量限分别为0.15 ng/mL和0.501 ng/mL。在肉类样品中未检测到AFB1。我们的结果表明,改进的监测肉类中AFB1水平的方法简单、省时、可靠。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Monitoring of Aflatoxin B1 in Dry Aged Meats using a modified HPLC-FLD method
The presence of aflatoxins residues in meat and meat products occurs through ingestion of feeds contaminated with Aflatoxin B1 (AFB1) or during the processing of the meat products. Little research has been conducted concerning the detection of AFB1 on meat. The aim of this study was to modify the method for AFB1 determination by high performance liquid chromatography with fluorescence detection in dry aged meats. The aged meat samples used for determination of AFB1 were obtained from a commercial dry aged meat plants and produced for 28 days in a meat plant chiller. All meat samples were analyzed according to the modified method. The samples were cleaned to remove impurities by using a immunoaffinity column. Photochemical reactor was used for the recording and evaluation of the chromatograms. The analytical performance parameters showed that the method was specific for AFB1 detection in the meat samples. Recovery rate, reproducibility (RSDr%) and repeatability (RSDR%) were found to be 85.889%, 3.822% and 6.601%, respectively. Limit of detection and limit of quantification were 0.15 ng/mL and 0.501 ng/mL, respectively. AFB1 was not detected in the meat samples. Our results indicated that the method modified for monitoring AFB1 level in the meats was simple, time-saving and reliable.
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来源期刊
Romanian Biotechnological Letters
Romanian Biotechnological Letters 生物-生物工程与应用微生物
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