26日龄鸡肠道内容物中乳酸菌的分离、鉴定、保存和测定

M. Dumitru, I. Sorescu, G. Ciurescu, M. Hăbeanu
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引用次数: 2

摘要

Chichens的胃肠道(GI)具有很大的细菌多样性,可以作为分离候选益生菌的有益来源。在本研究中,从16只鸡的回肠和盲肠消化液中分离、培养和保存了来自乳杆菌属的23株菌株(1株嗜酸乳杆菌生物型1、8株嗜酸乳菌生物型3、1株脆乳杆菌、5株发酵乳杆菌生物型2、2株唾液乳杆菌和1株德氏乳杆菌亚种),26天大,基于它们的表型特征。菌株确认通过apiwebTM API 50 CHL V.5.1、BioMerieux(法国)软件和ABIS在线软件进行。测定了选择性培养基中乳酸杆菌菌株(106-108CFU/g肠道含量)的水平。评估在4°C(48天至7个月)和室温(48天至90天)下保存的乳杆菌菌株的生长。这项研究表明,鸡可以成为分离可能具有活性的益生菌乳酸菌(LAB)的稳定来源。从分离和鉴定的乳杆菌菌株中,发酵乳杆菌生物型1和短乳杆菌生物类型2理论上分别适用于益生菌特性的连续测试。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation, identification, preservation and determinations of lactic acid bacteria from chicken’s gut content at 26 days old
Chichens’s gastrointestinal (GI) tract presents a large bacterial diversity and can be a beneficial source in order to the isolation of probiotics candidates. In this study, twentythree strains from Lactobacillus genus (one strain of L. acidophilus biotype 1, eight strains of L. acidophilus biotype 3, one of L. crispatus, five of L. fermentum biotype 1, two of L. brevis biotype 2, five of L. salivarius and one strain of L. delbrueckii subsp. delbrueckii) were isolated, cultured, and conserved from ileum and cecum digesta of sixteen chickens, 26 days old, based on their phenotypic characters. The strains confirmation was done by apiwebTM API 50 CHL V.5.1, BioMerieux (France) software, and ABIS online soft. The Lactobacillus strains (106 -108 CFU/g intestinal content) level in the selective medium were determined. The growth of Lactobacillus strains conserved at 4°C (from 48 days to 7 months) and room temperature (from 48 to 90 days) was evaluated. This study showed that chickens can be a stable source for isolating possibly active probiotic lactic acid bacteria (LAB). From Lactobacillus strains isolated and identified, respectively L. fermentum biotype 1 and L. brevis biotype 2 are theoretically suitable for continual testing of probiotic properties.
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来源期刊
Romanian Biotechnological Letters
Romanian Biotechnological Letters 生物-生物工程与应用微生物
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