Romanian Biotechnological Letters最新文献_第6页

In silico investigation of the potential interaction between two entomological peptides and human androgen receptor 两种昆虫学多肽与人类雄激素受体之间潜在相互作用的计算机研究

Romanian Biotechnological Letters Pub Date : 2021-04-11 DOI: 10.25083/RBL/26.3/2679-2684

Sorin Draga, Otopeni Ilfov Romania Biotehnos S.A., E. Buse, D. Ene, Sabina Serbu, B. Purcăreanu, L. Olariu

{"title":"In silico investigation of the potential interaction between two entomological peptides and human androgen receptor","authors":"Sorin Draga, Otopeni Ilfov Romania Biotehnos S.A., E. Buse, D. Ene, Sabina Serbu, B. Purcăreanu, L. Olariu","doi":"10.25083/RBL/26.3/2679-2684","DOIUrl":"https://doi.org/10.25083/RBL/26.3/2679-2684","url":null,"abstract":"Objective: We aim to evaluate the potential interaction of two insect hemolymph peptides, MDF3 and MDF4, with the human androgen receptor, on the premise that the proliferative effects of the two peptides are (at least in part) a consequence of AR binding. Methods: We employed a bioinformatic approach for the prediction of protein-peptide interaction and peptide aggregation, using various in silico on-line tools such as docking servers, aggregation prediction servers and visualization and analysis software in order to evaluate our results. Results: Our evaluation indicates that MDF3 and MDF4 interact with the androgen human androgen receptor by binding to a helix shown to be involved the receptor dimerization. Out of the two peptides, MDF3 appears to form a more extensive bond network with the receptor. Conclusion: Our analysis indicates that MDF 3 and 4 may be able to activate the human androgen receptor and warrant further investigation of the potential effect on receptor function. MDF3 appears to be the most promising out of the two peptides and its interaction should be further evaluated by both computational and experimental methods.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":"26 1","pages":"2679-2684"},"PeriodicalIF":0.0,"publicationDate":"2021-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46940444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of production conditions on gene expression levels of inulinase of Aspergillus wentii NRLL 1778 生产条件对文氏曲霉NRLL 1778菊粉酶基因表达水平的影响

Romanian Biotechnological Letters Pub Date : 2021-04-11 DOI: 10.25083/RBL/26.3/2650.2663

Ayten Bostanci, F. Sanal

引用次数: 0

Analysis of the agricultural labor force and its impact on agricultural products obtained in the South-Muntenia region 南蒙特尼亚地区农业劳动力及其对农产品获得的影响分析

Romanian Biotechnological Letters Pub Date : 2021-04-11 DOI: 10.25083/RBL/26.3/2640-2649

F. Teodorescu, L. Mărcuță, Cătălin Coman, V. Tudor

引用次数: 1

Production, purification and immobilization of inulinases from Aspergillius species 曲霉属菊粉酶的生产、纯化和固定化

Romanian Biotechnological Letters Pub Date : 2021-04-11 DOI: 10.25083/RBL/26.3/2685-2691

M. Eremia, Bucharest Romania Development-ICCF, M. Petrescu

{"title":"Production, purification and immobilization of inulinases from Aspergillius species","authors":"M. Eremia, Bucharest Romania Development-ICCF, M. Petrescu","doi":"10.25083/RBL/26.3/2685-2691","DOIUrl":"https://doi.org/10.25083/RBL/26.3/2685-2691","url":null,"abstract":"Inulinases are enzymes catalysing hydrolysis of polyfructosans to produce fructose or fructooligosaccharides; these properties have attracted interest of many researchers towards exploring various plant sources (agro food waste) as substrates. According to the literature, various microorganisms, such as fungi, yeast, bacteria, and actinomycetes, can synthesize inulinase. Producer microorganisms can be the micromycetes Aspergillus, Penicillium, Rhizopus and Fusarium, the yeast Kluyveromyces and the bacteria Clostridium thermosuccinogenes and Bacillus subtilis, using inulin, sucrose, fructose, lactose, raffinose, xylose as sources of carbon. The present study mainly envisages inulinase produced by Aspergillius species grown on a fermentation medium based on different carbon sources (inulin and agro-alimentary by-products). The crude extract was purified by fractional precipitation with ammonium sulphate. The dissolved and dialyzed precipitate was further purified by ion exchange chromatography on DEAE-Sephadex. The experiments led to the obtaining of an inulinase with a specific activity of 43.34 U/mg proteins. Inulinase immobilization tests on chitosan beads have led to a stable biocatalyst at temperature and pH variations that could be useful for obtaining fructose syrup.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":"26 1","pages":"2685-2691"},"PeriodicalIF":0.0,"publicationDate":"2021-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44041161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotechnological potential of plant associated microorganisms 植物相关微生物的生物技术潜力

Romanian Biotechnological Letters Pub Date : 2021-04-11 DOI: 10.25083/RBL/26.3/2700-2706

S. Fendrihan, C. Pop

引用次数: 12

Correlation between DTI effect and white matter structural integrity, working memory in leukoaraiosis patients 脑白质病变患者脑白质结构完整性、工作记忆与DTI效应的相关性研究

Romanian Biotechnological Letters Pub Date : 2021-04-11 DOI: 10.25083/RBL/26.3/2714-2721

Xiaofeng Yang, Wanmeng Xie

{"title":"Correlation between DTI effect and white matter structural integrity, working memory in leukoaraiosis patients","authors":"Xiaofeng Yang, Wanmeng Xie","doi":"10.25083/RBL/26.3/2714-2721","DOIUrl":"https://doi.org/10.25083/RBL/26.3/2714-2721","url":null,"abstract":"Our objective was to study the correlation between Diffusion tensor MR imaging (DTI) effect and white matter structural integrity, working memory in leukoaraiosis patients. 100 leukoaraiosis patients referring to the First Affiliated Hospital of Beijing Medical University from December 2018 to December 2019, were selected as study subjects and divided into four groups according to disease severity: lesion-free group, mild lesion group, moderate lesion group, and severe lesion group. All patients underwent magnetic resonance diffusion tensor imaging to collect DWI images and analyze Fractional anisotropy (FA), mean diffusivity (MD), ReHo values of white matter area under different grading. The patients’ working memory was tested via auditory verb learning test and Stroop color word test, so that correlation between white matter structural integrity and working memory can be analyzed. Results: There are statistically significant differences in FA values of the right posterior thalamic radiation, the right sagittal layer and the right superior longitudinal fasciculus, MD values of the right sagittal layer, the right cingulum bundle, the left cingulum bundle, the right inferior fasciculus fronto-occipitalis and the left inferior fasciculus frontooccipitalis, as well as instant recall, delayed recall, delayed recognition, card A (dot), card B (character), card C (color word) and SIE value (P<0.01). Correlation is shown between white matter structural integrity and working memory, gender, age, grading, disease course, recurrence interval, white matter area, and testing methods. There was a correlation between DTI effect and white matter structural integrity, working memory in leukoaraiosis patients, and leukoaraiosis patients have memory impairment.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":"26 1","pages":"2714-2721"},"PeriodicalIF":0.0,"publicationDate":"2021-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41630696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Review: Klebsiella pneumoniae, Klebisellaoxytoca and Biotechnology 肺炎克雷伯菌、氧化克雷伯杆菌与生物技术综述

Romanian Biotechnological Letters Pub Date : 2021-04-11 DOI: 10.25083/RBL/26.3/2567.2586

C. Tomulescu, M. Moscovici, I. Lupescu, R. Stoica, A. Vamanu

{"title":"A Review: Klebsiella pneumoniae, Klebisellaoxytoca and Biotechnology","authors":"C. Tomulescu, M. Moscovici, I. Lupescu, R. Stoica, A. Vamanu","doi":"10.25083/RBL/26.3/2567.2586","DOIUrl":"https://doi.org/10.25083/RBL/26.3/2567.2586","url":null,"abstract":"Biotechnology, molecular biology and genetic engineering, and bioprospecting play a crucial role in our common future, enabling industrially important microorganisms to ensure sustainable products (fuels, chemicals, pharmaceuticals, food, drug delivery systems, medical devices etc.) and new bioeconomic opportunities. Biotechnological applications are able to provide cost-effective green alternatives to conventional industrial processes, which are currently affecting the nature and biodiversity. Klebsiella species are among the well-studied microbes both in medicine field, as ones of the most resilient opportunistic pathogens, and in industry, due to their promising biochemical properties, and their potential as better hosts than other microorganisms, for i.e. in genetic manipulation. Klebsiella oxytoca and Klebsiella pneumoniae are ubiquitously found in natural environments, but also as commensals in the human gut, and associated with a high-resistance to the first-line antibiotics. However, these specific strains are continuously isolated and studied for different industrial purposes (i.e. bulk chemicals and biofuels production, medical diagnosis, nanoparticles and exopolysaccharides synthesis, plant growth promoting activities, bioremediation and biodegradation agents etc.), and scientific results regarding their biotechnological potential could generate big impact for global bioeconomy development. Recently, research in synthetic biology gained a lot of attention, and new techniques highlight ways to reprogramme these microbial cells in view of high-yield or high-quality new chemicals obtainment. Therefore, some scientific research niches are emerging in biotechnology, and unknown metabolic pathways and genes are identified and further studied, to provide alternative solutions to the global challenges.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":"26 1","pages":"2567-2586"},"PeriodicalIF":0.0,"publicationDate":"2021-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44113539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Congenital diaphragmatic hernia: a retrospective study regarding the clinical experience of Bihor County Genetics Center 先天性膈疝:比霍尔县遗传中心临床经验的回顾性研究

Romanian Biotechnological Letters Pub Date : 2021-04-11 DOI: 10.25083/RBL/26.3/2671-2678

C. Vesa, E. Severin, M. Bembea, A. Popa, Alina Sklerniacof, Claudia Jurca, K. Kozma, C. Daina, A. Jurcă, C. Petcheși, D. Galușca, S.-C. Jurca, D. Zaha, Romania Pharmacy “Carol Davila” Bucharest

{"title":"Congenital diaphragmatic hernia: a retrospective study regarding the clinical experience of Bihor County Genetics Center","authors":"C. Vesa, E. Severin, M. Bembea, A. Popa, Alina Sklerniacof, Claudia Jurca, K. Kozma, C. Daina, A. Jurcă, C. Petcheși, D. Galușca, S.-C. Jurca, D. Zaha, Romania Pharmacy “Carol Davila” Bucharest","doi":"10.25083/RBL/26.3/2671-2678","DOIUrl":"https://doi.org/10.25083/RBL/26.3/2671-2678","url":null,"abstract":"Congenital diaphragmatic hernia is an abnormality of embryologic development characterized by the persistence of communication between the thoracic and abdominal cavities, followed by an extremely high death rate despite medical progress. In Bihor county, according to a retrospective analysis of patient records from the Regional Center of Genetics, 30 children were diagnosed with congenital diaphragmatic hernia over a span of 28 years, from 1990 to 2018, most being male (73.3%). Predominant were the complex congenital diaphragmatic cases, meaning the cases of diaphragmatic hernia associated with other congenital abnormalities, 67% (20 patients) presenting at least one other congenital malformation. 95% (19 cases) of patients with complex congenital diaphragmatic hernia presented pulmonary abnormalities, the most frequent being pulmonary hypoplasia; 35% (7 cases) presented cardiac abnormalities. The Apgar score at 5 minutes after birth was significantly lower for patients with the clinical form of complex congenital diaphragmatic hernia. The mortality for the patients presenting the complex form was 100%, and for the patients presenting the isolated form was 20%. Karyotype analysis was performed for 17 patients, and 23.53% of them (4 patients) presented chromosomal abnormalities.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":"26 1","pages":"2671-2678"},"PeriodicalIF":0.0,"publicationDate":"2021-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48804157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation on the effects of different concentrations of some fertilizers on yield, quality and essential and fixed oil composition of Nigella damascena 不同浓度化肥对大麦产量、品质及精油和固定油成分影响的研究

Romanian Biotechnological Letters Pub Date : 2021-04-11 DOI: 10.25083/RBL/26.3/2722-2735

F. Ulusu, Alirıza Şahin

{"title":"Investigation on the effects of different concentrations of some fertilizers on yield, quality and essential and fixed oil composition of Nigella damascena","authors":"F. Ulusu, Alirıza Şahin","doi":"10.25083/RBL/26.3/2722-2735","DOIUrl":"https://doi.org/10.25083/RBL/26.3/2722-2735","url":null,"abstract":"In this study, the effect of application of fertilizers with different ingredients in different doses in cultivation of Nigella damascena plant on morphological, yield and quality was investigated. According to the obtained results; plant height was 25.07-37.54 cm, and the number of branches per plant was on average 2.33-5.60, besides, it has been observed that root lengths vary between 4.33-8.62 cm. On the other hand, while the average amount of capsules per plant was between 3.53-7.10, the capsule width and length varied by 12.92-15.06 (mm) and 13.85-16.29 (mm), respectively. When evaluated in terms of seed yield, approximately 45.68-113.57 kg da-1 yield has been obtained also, seed weights in the capsule are 0.104-0.158 g, thousand grain weights 2.31-2.84 g and seed weights per plant ranged from 32 to 0.87 g/plant. While the fixed oil rate were seen between 34-36.48% in the application groups, the fixed oil yield was calculated as 15.53- 41.43 kg da-1 . As a result of GC-MS analysis, it was observed that the main compound of essential oils was between 19.47-61.13% β-elemene (sesquiterpen), followed by 6.91-34.53% damascenine (alkaloid). In addition, 17 fatty acids were observed in GC-FID analysis and it showed that fertilizer applications cause a variable effect on saturated and unsaturated fatty acid compositions.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":"26 1","pages":"2722-2735"},"PeriodicalIF":0.0,"publicationDate":"2021-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42057014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Dental biofilm-induced gingivitis in children and adolescents. A literature review 儿童和青少年牙齿生物膜引起的牙龈炎。文献综述

Romanian Biotechnological Letters Pub Date : 2021-04-11 DOI: 10.25083/RBL/26.3/2664-2670

S. Popa, B. Pharmacy, S. Păunică, M. Giurgiu, D. Bodnar, I. Suciu, A. Totan, A. Dumitriu, A. Didilescu

引用次数: 1