Cytotoxicity evaluation and physicochemical properties of collagen isolated from silver carp tails as potential biomaterial

P. Preda, A. Enciu, M. Avram, C. Tanase, M. Dudău, I. Mihalache, O. Tutunaru, D. Stan, A. Ficai
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引用次数: 2

Abstract

Collagen is widely used as a biomaterial in the pharmaceutical and cosmetic industries, in the production of hydrogels, wound dressings, bioactive nano/microfibers, controlled drug delivery systems, etc. The collagen isolated from the aquatic source has a higher biological activity and low risk of transmitting genetic diseases. In recent years the sustainable socio-economic and environmental principles promote the full use of natural resources. Thus, fish collagen extracted from fish by-products (skin, scales, bones and fins) can be valorized as a new collagen alternative source. In this work the enzymatic hydrolysis with pepsin of collagen isolation from silver carp (Hypophthalmichthys molitrix) tails fish has been investigated. We successfully isolated type I collagen with 90-95% purity as determined by FTIR, UV-Vis, EDX and SDS-PAGE analyses. The cytotoxicity of obtained collagen was evaluating by MTS assays.
鲢鱼尾胶原蛋白潜在生物材料的细胞毒性评价及理化性质
胶原蛋白被广泛用作制药和化妆品行业的生物材料,用于生产水凝胶、伤口敷料、生物活性纳米/微纤维、可控药物递送系统等。从水生来源分离的胶原蛋白具有更高的生物活性和传播遗传病的低风险。近年来,可持续的社会经济和环境原则促进了自然资源的充分利用。因此,从鱼类副产品(皮肤、鳞片、骨骼和鳍)中提取的鱼类胶原蛋白可以作为一种新的胶原蛋白替代来源。本文研究了胃蛋白酶对鲢鱼尾鱼胶原蛋白的水解作用。通过FTIR、UV-Vis、EDX和SDS-PAGE分析,我们成功分离出纯度为90-95%的I型胶原。通过MTS测定来评价所获得的胶原的细胞毒性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Romanian Biotechnological Letters
Romanian Biotechnological Letters 生物-生物工程与应用微生物
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