P. Preda, A. Enciu, M. Avram, C. Tanase, M. Dudău, I. Mihalache, O. Tutunaru, D. Stan, A. Ficai
{"title":"Cytotoxicity evaluation and physicochemical properties of collagen isolated from silver carp tails as potential biomaterial","authors":"P. Preda, A. Enciu, M. Avram, C. Tanase, M. Dudău, I. Mihalache, O. Tutunaru, D. Stan, A. Ficai","doi":"10.25083/rbl/26.4/2808-2817","DOIUrl":null,"url":null,"abstract":"Collagen is widely used as a biomaterial in the pharmaceutical and cosmetic industries, in the production of hydrogels, wound dressings, bioactive nano/microfibers, controlled drug delivery systems, etc. The collagen isolated from the aquatic source has a higher biological activity and low risk of transmitting genetic diseases. In recent years the sustainable socio-economic and environmental principles promote the full use of natural resources. Thus, fish collagen extracted from fish by-products (skin, scales, bones and fins) can be valorized as a new collagen alternative source. In this work the enzymatic hydrolysis with pepsin of collagen isolation from silver carp (Hypophthalmichthys molitrix) tails fish has been investigated. We successfully isolated type I collagen with 90-95% purity as determined by FTIR, UV-Vis, EDX and SDS-PAGE analyses. The cytotoxicity of obtained collagen was evaluating by MTS assays.","PeriodicalId":21566,"journal":{"name":"Romanian Biotechnological Letters","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Romanian Biotechnological Letters","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.25083/rbl/26.4/2808-2817","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
Collagen is widely used as a biomaterial in the pharmaceutical and cosmetic industries, in the production of hydrogels, wound dressings, bioactive nano/microfibers, controlled drug delivery systems, etc. The collagen isolated from the aquatic source has a higher biological activity and low risk of transmitting genetic diseases. In recent years the sustainable socio-economic and environmental principles promote the full use of natural resources. Thus, fish collagen extracted from fish by-products (skin, scales, bones and fins) can be valorized as a new collagen alternative source. In this work the enzymatic hydrolysis with pepsin of collagen isolation from silver carp (Hypophthalmichthys molitrix) tails fish has been investigated. We successfully isolated type I collagen with 90-95% purity as determined by FTIR, UV-Vis, EDX and SDS-PAGE analyses. The cytotoxicity of obtained collagen was evaluating by MTS assays.