Research Journal of Pharmacy and Technology最新文献_第7页

In Silico Studies on the Antidiabetic activity of Avicularin 关于阿维菌素抗糖尿病活性的硅学研究

Research Journal of Pharmacy and Technology Pub Date : 2024-01-19 DOI: 10.52711/0974-360x.2024.00004

Neeli Parvathi, Rajitha Rajendran, S. Iyyam Pillai, Sorimuthu Pillai Subramanian

{"title":"In Silico Studies on the Antidiabetic activity of Avicularin","authors":"Neeli Parvathi, Rajitha Rajendran, S. Iyyam Pillai, Sorimuthu Pillai Subramanian","doi":"10.52711/0974-360x.2024.00004","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00004","url":null,"abstract":"Type 2 diabetes mellitus (T2DM) is a worldwide public health crisis. The existing treatments have limitations such as undesirable side effects such as hypoglycemia, unusual weight gain and the development of resistance after prolonged use, which necessitates the development of new therapies for paramount glycemic control, especially those of plant origin. Avicularin, a plant flavonoid and a quercetin glycoside, was originally purified from Psidium guajava. It has been reported to elicit a wide range of pharmacological and beneficial properties especially striking antidiabetic activity. In-silico models have been recognized as being of fundamental importance in the area of research and development of drugs due to their applications both in the evaluation of bioactive substances and in relation to their physicochemical and pharmacokinetic properties, giving rise to a new model of drug design with greater value and efficiency. The aim of the present study was to analyze the molecular interactions between Avicularin andprominent target proteins involved in the commencement and development of diabetes and its secondary complications using an in silico approach.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"73 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140503295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-epitopes Vaccine Design against Klebsiella pneumoniae based on Outer Membrane Protein using Immunoinformatics Approaches 利用免疫信息学方法设计基于外膜蛋白的肺炎克雷伯菌多位点疫苗

Research Journal of Pharmacy and Technology Pub Date : 2024-01-19 DOI: 10.52711/0974-360x.2024.00003

Indira Prakoso, Alfero Putra Iryanto, Tiara Rahayu, Anzillina Rahma, Muhammad Nur Aziz Ar Rizqi, V. D. Kharisma, Arif Nur Muhammad Ansori, M. Rebezov, P. Burkov, Marina Derkho, Belyakova Natalia, Rybakova Anna, V. Jakhmola, R. Zainul

{"title":"Multi-epitopes Vaccine Design against Klebsiella pneumoniae based on Outer Membrane Protein using Immunoinformatics Approaches","authors":"Indira Prakoso, Alfero Putra Iryanto, Tiara Rahayu, Anzillina Rahma, Muhammad Nur Aziz Ar Rizqi, V. D. Kharisma, Arif Nur Muhammad Ansori, M. Rebezov, P. Burkov, Marina Derkho, Belyakova Natalia, Rybakova Anna, V. Jakhmola, R. Zainul","doi":"10.52711/0974-360x.2024.00003","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00003","url":null,"abstract":"Klebsiella pneumoniae is a gram-negative of bacteria that are known to cause a variety of nosocomial respiratory tract infections including pneumonia. K. pneumoniae is also included in the ESKAPE bacteria group which has high resistance to antibiotics. Therefore, alternative treatment for K. pneumoniae infection is needed, one of which is by developing a vaccine. The aim of this study was to design a vaccine against K. pneumoniae by targeting the outer membrane protein using immunoinformatics approaches. 1,708 protein of K. pneumoniae was then screened using signalP, pred-TMBB2, and Blastp to select outer membrane proteins. The selected protein, PA1_KLEPN and BAMA_KLEP7 were then predicted using T-and B-cell Epitope Prediction on IEDB to obtain epitope regions. Vaccine design of K. pneumoniae consists of 1 BCL epitope, 2 CTL epitopes, 1 HTL epitope, an adjuvant and PADRE sequences constructed with linkers using Benchling. This vaccine construction is predicted to be non-toxic/allergenic and have a strong binding affinity with human TLR-4 with the HADDOCK score of -93.2kcal/mol, RMSD 0.5 and Z-score -2.5. According to the computer-aided studies conducted for this study, the chosen epitopes may provide excellent vaccine candidates to stop K. pneumoniae infections in people. However, in order to further confirm the efficacy of this suggested vaccine candidate, in vitro and in vivo validation is required.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140503087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metformin and Omega 3 effect on Cholesterol and HDL level in Iraqi women with Poly Cystic Ovary Syndrome 二甲双胍和欧米伽 3 对伊拉克多囊卵巢综合征妇女胆固醇和高密度脂蛋白水平的影响

Research Journal of Pharmacy and Technology Pub Date : 2024-01-19 DOI: 10.52711/0974-360x.2024.00060

Baraa Abd Alkareem, Huda I. Al-Qadhi, Fatima S. Salman

{"title":"Metformin and Omega 3 effect on Cholesterol and HDL level in Iraqi women with Poly Cystic Ovary Syndrome","authors":"Baraa Abd Alkareem, Huda I. Al-Qadhi, Fatima S. Salman","doi":"10.52711/0974-360x.2024.00060","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00060","url":null,"abstract":"PCOS is a prevalent endocrine condition affecting women in their reproductive years all over the world. Metabolic abnormalities in women with PCOS frequently include insulin resistance and dyslipidemia, which is characterized by low levels of HDL and increased cholesterol. Metformin, an insulin-sensitizing medication, is commonly used to treat PCOS. Omega-3 fatty acids, found in fish oil supplements, have been shown to have beneficial effects on lipid profiles and reduce inflammation, making them a potential complementary therapy for PCOS. This study aimed to evaluate the effect of a combination of metformin and omega-3 supplements on the lipid profiles of women with PCOS. This study aim to evaluate the effect of using metformin (1000mg/day) and omega 3(1000mg/day) for 3 consecutive months on cholesterol and HDL level (in prospective manner) of patient serum with PCOS. This study was performed at gynecology outpatient clinic and infertile center in AL-batol hospital during the period from December 2021 to march 2022. A total 59 women between (18-40years) with PCOS were randomly assigned into two group, the first group were taking metformin for 12 week and the second group were taking metformin and omega 3 for 12 week. There cholesterol and HDL were measure before and after the treatment .The result show that use of metformin and omega 3 is more significant (P value 0.281) in reducing the level of cholesterol and increase HDL(p value 0.627) than metformin alone (p value 0.525) and (p value 0.106) respectively.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"463 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140502578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-Step Maceration Extraction and Pharmacological Evaluation of Abelmoschus manihot (L.) Medik Abelmoschus manihot (L.) Medik 的多步浸渍萃取和药理评估

Research Journal of Pharmacy and Technology Pub Date : 2024-01-19 DOI: 10.52711/0974-360x.2024.00061

Ardina Purnama Tirta, Imas Solihat, Achmad Nandang Roziafanto, Nurhasanah Nurhasanah, Ratnawati L Djanis, Inda Mapiliandari

{"title":"Multi-Step Maceration Extraction and Pharmacological Evaluation of Abelmoschus manihot (L.) Medik","authors":"Ardina Purnama Tirta, Imas Solihat, Achmad Nandang Roziafanto, Nurhasanah Nurhasanah, Ratnawati L Djanis, Inda Mapiliandari","doi":"10.52711/0974-360x.2024.00061","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00061","url":null,"abstract":"A study has been conducted on the phytochemical profile and pharmacological activity of Gedi (Abelmoschus manihot (L.) Medik) using a multistage maceration method. The results of the phytochemical screening test showed that the crude extract of Gedi leaves gave a positive reaction to the presence of alkaloids, tannins, phenols, flavonoids, unsaturated steroids and saponins. The total phenolic analysis showed that the total phenolic concentrations of Gedi leaf extracts in methanol, ethyl acetate, and hexane extracts were 1.70, 1.44, and 0.85mg GAE/g extract, respectively. The results of the antioxidant activity assays obtained an IC50 value for methanol, ethyl acetate, and hexane extracts of Gedi leaves, which were 40.77, 155.50, and 25.84mg/L, respectively. The antimicrobial activity results showed that all three Gedi leaf extracts had activity in inhibiting microbial growth. The inhibitory values for methanol, ethyl acetate, and hexane extracts of Gedi leaves were 7.0, 13.5, and 9.5mm, respectively, for Escherichia coli, and 21.5, 15.0, and 13.0mm, respectively, for Bacillus. The toxicity test results showed that the LC50 values of Gedi leaf extracts for methanol, ethyl acetate, and hexane extracts were 500 ppm, 736ppm, and 2249ppm, respectively.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"463 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140502654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cutoff Point Measurement of the waist circumference for the diagnosis of Metabolic Syndrome in Iraqi university students 用于诊断伊拉克大学生代谢综合征的腰围临界点测量方法

Research Journal of Pharmacy and Technology Pub Date : 2024-01-19 DOI: 10.52711/0974-360x.2024.00020

Alaa Hasoon Zamil, Seenaa Sadiq Amin

引用次数: 0

In Vitro Evaluation of Alpha Amylase and Alpha Glucosidase Inhibitory Activity of Kamadhenu Ark extracts of Climate Smart Crop Millets 体外评价卡玛德努方舟提取物对气候智能型作物黍稷的α-淀粉酶和α-葡萄糖苷酶的抑制活性

Research Journal of Pharmacy and Technology Pub Date : 2024-01-19 DOI: 10.52711/0974-360x.2024.00013

Venkata Lakshmi J, Ch. M. Kumari Chitturi, C. A. Rao

{"title":"In Vitro Evaluation of Alpha Amylase and Alpha Glucosidase Inhibitory Activity of Kamadhenu Ark extracts of Climate Smart Crop Millets","authors":"Venkata Lakshmi J, Ch. M. Kumari Chitturi, C. A. Rao","doi":"10.52711/0974-360x.2024.00013","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00013","url":null,"abstract":"Diabetes Mellitus is characterized by abnormal Metabolism of glucose. The maintenance of glucose levels in blood without any side effects is still challenging in the medication of this disease. Various medical approaches like Allopathy, Homeopathy, Ayurveda, Unani etc are striving a lot for success in this field of medication. Present study is aimed to evaluate the Antidiabetic activity of Millets in combination with Kamadhenu Ark as solvent for the probable alpha Amylase and alpha Glucosidase inhibitory activities to reduce hyperglycemia. The Millets namely Paspalum scrobiculatum, Panicum miliaceum, Panicum sumatrense were selected for the study. The Kamadhenu Ark Extracts were prepared by Maceration method. The extracts were used for the estimation of alpha amylase and alpha-glucosidase inhibition activity. Extract of Panicum miliaceum exhibited highest α amylase and α glucosidase inhibitory potential with IC50 values of 0.239 and 1.526 mg/ml, respectively, which was comparable with acarbose (0.258 and 1.985 mg/ml). Extracts of Paspalum scrobiculatum and Panicum sumatrense have shown low activity. Present study paves a new path revealing the effective inhibition of α amylase and α glucosidase by Kamadhenu Ark extract of Panicum miliaceum (Proso Millet KPM).","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"487 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140502701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum Levels of Fetuin-A, Ischemia-modified Albumin (IMA), and Ferritin in Hospitalized patients with severe COVID-19. A Case-control Study 重症 COVID-19 住院患者血清中的 Fetuin-A、缺血修饰白蛋白 (IMA) 和铁蛋白水平。病例对照研究

Research Journal of Pharmacy and Technology Pub Date : 2024-01-19 DOI: 10.52711/0974-360x.2024.00063

Marwa Younus Fadhil, Eman Saadi Saleh

{"title":"Serum Levels of Fetuin-A, Ischemia-modified Albumin (IMA), and Ferritin in Hospitalized patients with severe COVID-19. A Case-control Study","authors":"Marwa Younus Fadhil, Eman Saadi Saleh","doi":"10.52711/0974-360x.2024.00063","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00063","url":null,"abstract":"Objective: To measure the serum levels of Fetuin-A, ischemia-modified albumin (IMA), and ferritin in hospitalized patients with severe COVID-19in Baghdad, Iraq. Moreover, to determine these biomarkers' cut-off valuesthat differentiate between severely ill patients and control subjects. Methods: This case-control study was done from 15 September to the end of December 2021 and involved a review of the files and collectionof blood samples from patients (n=45, group1) hospitalized in COVID-19 treatment centersbecause of severe symptoms compared tohealthy subjects as controls (n=44, group2). Results: Fetuin-A serum levels were not statistically different between patients and controls. In contrast, IMA and ferritin levels were significantly different between the 2 groups, with patients' levelsbeing greater than control participants' (p 0.05). The critical values for the Fetuin-A, IMA, and ferritin tests were 393.78 mg/L, 59.22 ng/ml, and 126 µg/L, respectively, with concentration curves of 0.58, 0.70, and 0.93 for each. Conclusions: Patients and controls showed no significant difference in Fetuin-A levels in the blood. However, IMA and ferritin levels werehigher in people suffering from acute COVID-19 infection than in controls, with Fetuin-A values less than 393.78 mg/L andIMA and ferritin valueshigher than 59.22 ng/mland 126,000 μg/L, respectively.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"15 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140503313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HPTLC Analysis of Senna occidentalis (L.) in the Methanolic extract of the leaf 西洋番泻叶甲醇提取物的 HPTLC 分析

Research Journal of Pharmacy and Technology Pub Date : 2024-01-19 DOI: 10.52711/0974-360x.2024.00037

Santosh Kumar, Shikhar Verma, Pritt Verma, Anant Kumar Shrivastav

引用次数: 0

Impact of Breathing exercise and Inhalation Technique on patient’s Quality of Life among Asthma and COPD patients 呼吸运动和吸入技术对哮喘和慢性阻塞性肺病患者生活质量的影响

Research Journal of Pharmacy and Technology Pub Date : 2024-01-19 DOI: 10.52711/0974-360x.2024.00057

SM. Biradar, C. V. Kumar, K Sainadh, NS Vinay Kumar, P. Avinash, AP. Ambali, Shashidhar Devaramani, Akram Naikwadi, Abhishek B, SR. Awasthi, Prashanth Jorapur

{"title":"Impact of Breathing exercise and Inhalation Technique on patient’s Quality of Life among Asthma and COPD patients","authors":"SM. Biradar, C. V. Kumar, K Sainadh, NS Vinay Kumar, P. Avinash, AP. Ambali, Shashidhar Devaramani, Akram Naikwadi, Abhishek B, SR. Awasthi, Prashanth Jorapur","doi":"10.52711/0974-360x.2024.00057","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00057","url":null,"abstract":"Around 4 million deaths each year are caused by respiratory disorders worldwide. These diseases are a persistent threat to India as well; the number of people in India who suffer from COPD and asthma has been estimated to be 14.84 million and 17.23 million, respectively.In the present prospective and observational study, a total of 120 patients were enrolled, among 38 patients were diagnosed with Asthma and 82 were COPD patients. All the patients were trained with breathing exercises and inhalation techniques. The patients who have been prescribed with DPI and MDI inhalation devices were checked for their correct usage. The checklist score (DPI and MDI) for Asthma patients, before trainingwas 2.9 and after training was 8.1(<0.001***) and checklist score for COPD patients, before training was 2.8 and after training was 8.5(<0.001***).The results indicate that there is a significant increase (<0.001***) in correct usage after training session, which influences the inspiratory flow rate on Respirometer. Asthma Patient’s QoLwas measured by using ACT score, before training was 9.3 and after training was 19.7(<0.001***) and AQLQ score before training was 35.6 and after training was 74.1 (<0.001***). For COPD Patient’s QoLwas measured by using CAT score, before training was 33.2 and after training was 12.1(<0.001***) and MMRC score before training was 3.6 and after training was 1.4(<0.001***). In the present study breathing exercises increases inhalation capacity and hands on training for inhalation techniques improves the efficacy of drugs, together influences the patient’s Quality of Life (QoL) which reduces the number of days of hospitalization and socio-economic burden.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"77 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140503387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of Red Algae (Eucheuma denticulatum (Burman) Collins et Harvey) Extract Bioactive Compounds using Liquid Chromatography Mass Spectroscopy - Mass Spectroscopy 利用液相色谱质谱法研究红藻（Eucheuma denticulatum (Burman) Collins et Harvey）提取物中的生物活性化合物 - 质谱法

Research Journal of Pharmacy and Technology Pub Date : 2024-01-19 DOI: 10.52711/0974-360x.2024.00056

Syamsuri Syakri, Sartini Sartini, Upik A. Miskad, A. Aminuddin

{"title":"Study of Red Algae (Eucheuma denticulatum (Burman) Collins et Harvey) Extract Bioactive Compounds using Liquid Chromatography Mass Spectroscopy - Mass Spectroscopy","authors":"Syamsuri Syakri, Sartini Sartini, Upik A. Miskad, A. Aminuddin","doi":"10.52711/0974-360x.2024.00056","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00056","url":null,"abstract":"Research has been carried out on the Study of Red Algae (Eucheuma denticulatum (Burman) Collins et Harvey) Extract Bioactive Compounds Using Liquid Chromatography Mass-Mass Spectroscopy (LCMS-MS). This study aims to determine the bioactive compounds produced in this red algae extract by means kinetic maceration of extraction. Reading of bioactive compounds using LCMS-MS to obtain compound results, compound structures, and molecular weights. The results show that there are 22 bioactive compounds contained in this plant. Consist of : 4-Dodecylbenzenesulfonic acid, NP-009092, 6-Hydroxycaproic acid, Xanthine, 3-Phenyilactic acid, Azelaic acid, Apro barbital, Decosahexaenoic acid, Methil hydrogen Phenylphoshonate, (1S)-1-Carboxy-N,N,N-trimethyl-2-(2-thioxo-2,3-dihydro-1H-imidazol-4-yl)ethanaminium, D-(+)-Tryptophan, uracil, ?17-6-keto Prostaglandin F1, Hypoxanthine, 20-hydroxy Prostaglandin F2?, 2-Hydroxy-4-methylthiobutanoic acid, N-(2-Furylmethyl)-3-[(2S,5aS,8aR)-1-methyl-5-oxodecahydropyrrolo[3,2-E][1,4]diazepin-2-yl]propanamide, 6-Hydroxycaproic acid, Methaphenilene, 15(S)-HpEPE, [(4Z)-5-(Methylsulfanyl)-4-penten-2-yn-1-yl]benzene, Suberic acid.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"77 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140503396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0