Research Journal of Pharmacy and Technology最新文献

{"title":"Construction of Peptide Vaccine Candidate Based on β-Cell Epitopes of Indonesian Monkeypox Virus (MPXV) Virulence Protein:A Reverse Vaccinology","authors":"Viol Dhea Kharisma, A. Ansori, Ahmad Affan Ali Murtadlo, Muhammad Hermawan Widyananda, Md. Emdad Ullah, Sin War Naw, V. Jakhmola, K. Dobhal, Tarun Parashar, M. Rebezov, R. Zainul","doi":"10.52711/0974-360x.2024.00045","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00045","url":null,"abstract":"Infection with a DNA virus called monkeypox virus (MPXV) in humans has been identified in the Congo since 1970. Antiviral drugs are not effective for preventing MPXV infection. MPXV infection cases in Indonesia are very low but MPXV has the potential to become a global pandemic so it is very important to do prevention such as vaccine development. This study aims to construct a B cell epitope-based peptide vaccine candidate in Indonesian MPXV through an in silico approach.The development of the MPXV vaccine can be performed through a computational approach for preliminary studies. In silico-based construction of vaccines using B cell epitopes, antigenicity, allergenicity, docking, and molecular dynamics analysis have been used by researchers and scientists in solving viral infection cases. We recommend Pep A and Pep D as vaccine candidates because they allow recognition by B cells, antigenic peptides, non-allergenic and non-toxin. Peptide vaccine candidate can trigger B-cell activation to produce IgM isotype-specific antibodies through BCR interaction. In summary, the results of this study can be used for an initial study of MPXV vaccine development in Indonesia.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"75 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140503417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation Between the Severity of Chronic Periodontitis and Coronary Artery Stenosis Involvement in Coronary Heart Disease (CHD) Patients","authors":"Sanggap Indra Sitompul, Budi Susetyo Pikir, A. Aryati, Shafira Kurnia Supandi, Monika Estherlita Sinta","doi":"10.52711/0974-360x.2024.00035","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00035","url":null,"abstract":"Periodontitis is independently related to coronary heart disease and significantly impacts the quality of human life. The aim of this study was to investigate the relationship between the severity of chronic periodontitis and the severity of coronary artery stenosis in CHD patients. A total of 75 subjects were recruited for this study. The severity of the coronary artery was measured by coronary angiography. Quantitative coronary angiography (QCA) was conducted to determine 1 vessel disease (VD), 2 VD, and 3 VD, followed by stenosis. The severity of chronic periodontitis was determined based on the mean periodontal pocket depth (Mean PPD). The < 4 mm and 4 mm scores were categorized as mild and moderate-severe, respectively. Statistical analysis was conducted to determine the relationship between periodontitis and coronary artery by chi-square and followed by multivariate analysis using an ordinal regression test with a 95% confidence interval. P value < 0.05 was considered statistically significant. The proportion of moderate-severe periodontitis was more dominant than mild periodontitis. 79.20% and 20.80% of subjects were categorized as moderate-severe periodontitis and mild periodontitis in the 3 VD group. In groups 1 and 2 VD, the proportion of mild periodontitis was higher than moderate-severe periodontitis (61.30% vs. 38.7%) and (70% vs. 30%), based on bivariate analysis of the involvement of the coronary artery. Multivariate analysis showed chronic periodontitis and dyslipidemia had estimates of 1.084 (95% CI range 0.162 - 2.006) and 1.141 (95% CI range 0.217 - 2.066) for the severity of coronary artery involvement with p-values of 0.021 and 0.016, respectively. Gender, age, smoking, DM, hypertension, and obesity were not statistically significant. A significant relationship between moderate-severe chronic periodontitis was found, followed by an increase in the severity of coronary artery compared to mild chronic periodontitis.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"72 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140503430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Identified risks in the release of over-the-counter medicines to Teenagers","authors":"Anastasia Sinitsyna, Maria Denisova, Tatyana Litvinova, Irina Glazkova, O.V. Savinova, Dmitrii Babaskin","doi":"10.52711/0974-360x.2024.00046","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00046","url":null,"abstract":"Despite the absence of prohibiting legal norms in Russia, pharmaceutical workers often refuse to sell over-the-counter medicines to teenagers, guided by the unspoken rules of the pharmacy or the internal moral and ethical code. The purpose of this study was to identify and assess the main types of risks faced by pharmaceutical workers when dispensing medicines to teenagers. The assessment and identification of risks were carried out using the Delphi methods and a questionnaire in which 186 respondents took part (the representativeness of the sample was calculated using the statistical method). The probability of occurrence of risks and the degree of criticality of the consequences of the risk was assessed. The experts stated that the most likely event would be a teenager buying the wrong medicine (a medicine other than the one that the teenager or the persons who asked them to buy it were planning to obtain), with a different trade name, dosage, or formulation, followed by a possible conflict with the child's representatives with a request to return or exchange the medicine. Besides, highly probable events include the use of information obtained from unverified sources, including peer advice and information obtained from social networks, by the teenager. The experts named the purchase of medicines by teenagers for self-poisoning the most critical risk factor. The experts considered the risk of using psychoactive substances an event with a high degree of consequences. The experts considered the improper use of medicines by teenagers to be the least risky event. The risks were ranked using a risk matrix by the following categories: negligible, acceptable, moderate, significant, and unacceptable.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140503193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antidiabetic activity of Akar Kuning (Fibraurea tinctoria Lour) Extract in Alloxan-Induced Diabetic White Male Rats","authors":"S. Supomo, Eka Siswanto Syamsul, Hayatus Saadah, K. Kintoko, Hardi Astuti Witasari","doi":"10.52711/0974-360x.2024.00059","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00059","url":null,"abstract":"The Akar kuning (Fibraurea tinctoria Lour) is a well-known plant for its potential for the treatment of liver damage and hyperglycemia. However, the main constituent responsible for the anti-diabetic effect is unknown. This study was conducted to investigate the effect of administration of Fibraurea tinctoria Lour ethanol extract on the blood sugar levels in alloxan-induced diabetic male albino rats. All animals were acclimatized with normal feed, and were subsequently divided into 5groups (3rats/ group). Group 1 (negative control group) was given 0.3% NaCMC; Groups 2, 3, and 4 (treatment groups), were given 50, 100, and 200mg/kg BW of Fibraurea tinctoria Lour ethanol extract, respectively once daily for 15 days, while Group 5(positive control group) received 0.65mg/kg BW glibenclamide. Before the treatments, the rats were induced intraperitoneally with 175mg/kg BW alloxan monohydrate. The blood glucose levels were observed every five days. The results showed that the ethanol extract at the doses of 50, 100, and 200mg/kg BW caused a significant decrease (p <0.05) in the blood glucose levels compared to the negative control group. Also, the highest decrease in the blood glucose level was observed in the 100mg/kg BW treatment group. The findings from this study indicate that the ethanol extract of Fibraurea tinctoria Lour has an antidiabetic effect and the 100mg/kg BW dose was the most effective in lowering blood glucose levels.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"3 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140503227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of Bay Leaf Decoction on reducing Blood pressure in Hypertension patients in The working area of South Lasalimu","authors":"Dewi Sari Pratiwi, Armayani Armayani, Mimi Yati, Ruma Poddar","doi":"10.52711/0974-360x.2024.00027","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00027","url":null,"abstract":"Introduction: South Lasalimu Public Health Center is the third-ranked public health centre in Buton Regency in terms of hypertension prevalence over the last three years. The non-pharmacological treatment of bay leaf decoction in hypertension in South Lasalimu has yet to be developed with the goal of reducing hypertension in hypertension patients in South Lasalimu's working area. Methods: Study used a pre-experimental design. The sample size was 26 people because the population consisted of 35 hypertension patients. The information was gathered between June 3 and July 15, 2022. In order to test the effect of bay leaf decoction, this study used the Wilcoxon test. Results: The study's systolic and diastolic results in hypertension patients were 0.00(p 0.05). Conclusion: There is an effect of bay leaf decoction in the working area of South Lasalimu Public Health Center. It is hoped that hypertension patients can consume a bay leaf decoction if their blood pressure increases.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"78 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140502479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Rhizophora apiculata barks extract on Cholesterol, Triglyceride, LDL, and HDL Levels of Rattus norvegicus (Sprague Dawley) fed high-cholesterol diet","authors":"Syazili Mustofa, Andi Nafisah Tendri Adjeng, E. Kurniawaty, Lovina Ramadhita, Tania Tamara","doi":"10.52711/0974-360x.2024.00062","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00062","url":null,"abstract":"Background:Hypercholesterolemia is a lipid metabolic condition associated with cardiovascular disease (CVD), the leading cause of death worldwide. Rhizophora apiculata is a plant that grows along the Indonesian coast and may provide significant support for using traditional medicine. Bioactive molecules extracted from Rhizophora apiculta can be used in supplementary medicines and considered to be an alternative way to address the ever-increasing requirements of novel drugs. More research is needed to substantiate the usage of Rhizophora apiculate astraditional medicine.This study aims to investigate the effects of Rhizophora apiculata barks (RAB) ethanolic extract on lipid metabolism, namely Total Cholesterol (TC), Triglyceride (TG), LDL, and HDL levels in mice fed a high-cholesterol diet (HCD). Methods: The study comprised 30 white male rats (Rattus norvegicus) of the Sprague Dawley strain, aged 2-3 months and weighing 200-250 grams. Six groups of mice (n = 5) were formed. Group 1 was fed a conventional diet; Group 2 was given the High-Cholesterol Diet (HCD); Group 3 was given HCD and simvastatin 40 mg/kgbwt (p.o. ); and Groups 4, 5, and 6 were treated with HCD and RAB extract 56.55 mg/kgbwt (p.o.), RAB 28.28 mg/kgbwt (p.o.), and RAB 14.14 mg/kgbwt. All of the treatments began on the same day and lasted 30 days. Results: RAB-treated animal groups (56.55; 28.28; and 14.14 mg/kgbwt) had markedly lessened TC levels by 41.18%, 35.57%, and 9.24%, respectively; TG levels declined by 45.85%; 43.77%; and 27.74%, respectively; LDL levels reduced by 26.08%; 16.55%; and 11.21%, respectively; and HDL levels increased by 19.37%; 15.12%; and 13.21%, respectively. Conclusion: RAB-treated animal groups (56.55; 28.28; and 14.14 mg/kg) had markedly lessened total cholesterol, triglyceride, LDL and increased HDL levels (p < 0.01) related to the HCD alone batch. These findings imply that the ethanolic extract of Rhizophora apiculata barks influences lipid metabolism and has potency as traditional medicine.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"102 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140502550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"User Friendly Analytical Method Development and Validation of Salmeterol Xinafoate an Antiasthmatic Drug in Bulk and Formulated Product","authors":"Sonal Nalkar, S. Sutar, Mayuresh. R. Redkar, Priyanka More, Pooja Swami","doi":"10.52711/0974-360x.2024.00055","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00055","url":null,"abstract":"A simple, precise, accurate, sensitive cheap and selective RP-HPLC method has been developed of salmeterol in bulk and pharmaceutical formulation. The method was carried out on an Inertsil ODS C18 (250 x 4.6mm, packed with 5µ). The maximum absorbance was found to be at 203nm. The Salmeterol xinafoate followed linearity in the concentration range 20 to 70ppm and correlation coefficient was found to be 0.999. Injection volume was selected to be 20µl and the flow rate was fixed at 1.0ml/min. Mobile phase selected with the ratio of methanol and water (60:40 v/v). Run time was selected to be 8min. Retention team was found to be 3.521. The LOD and LOQ were found to be 0.00471 and 0.01429.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"60 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140502943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Green UPLC Method for the Simultaneous determination of Tenofovir and Amoxicillin in Biological Fluids and Dosage Forms","authors":"Shaimaa Elsayed, A. El-Gindy, yasser el-shabrawy, ElSayed A. Ibrahim","doi":"10.52711/0974-360x.2024.00012","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00012","url":null,"abstract":"This paper elucidates the process of developing and validating the UPLC technique, aiming to enable the concurrent measurement of tenofovir and amoxicillin. This UPLC method is accurate and sensitive. The experimental setup involved utilizing a Kinetex 1.7μ C18 100A (2.1-mm × 50-mm) column maintained at a temperature of 30°C. The mobile phase employed a combination of methanol and phosphate buffer (0.1% orthophosphoric acid in water, pH 3.5) in a ratio of 30:70 (v/v). The flow rate was set at 0.2mL/min, and the detection wavelength for analysis was 230nm. All these conditions resulted in optimum chromatographic separation. This developed method of UPLC took less than 4 minutes to separate this mixture. The detection limits for two medications were determined to be 0.45µg/mL for tenofovir and 0.26µg/mL for amoxicillin. The calibration plots of both substances exhibited satisfactory linearity within the concentration range of 1.0 - 2.0 µg/mL. Remarkably, the method demonstrated excellent performance with high percentage recoveries ranging from 96.8% to 102.92% and low percentage relative standard deviation (%RSD) values below 2%. Consequently, the proposed methodology proved to be highly effective for detecting the targeted drugs in their respective dosage forms.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"14 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140503316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MMP-7 Levels Decrease in Gingival Epithelium Exposed to Porphyromonas Gingivalis after Curcumin administration","authors":"A. Krismariono, Carolyn Purnama S","doi":"10.52711/0974-360x.2024.00048","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00048","url":null,"abstract":"Periodontitis is an irreversible inflammatory disease of the periodontal tissues. The leading cause of periodontitis is Porphyromonas gingivalis bacteria, which secretes virulence factors, namelyLipopolysaccharide (LPS) and proteases, which cause the inflammation of gingival junctional epithelium and loss of attachment, resulting in the formation of a periodontal pocket. The most common type of MMP found in the junctional epithelium is MMP-7. Invasion of Porphyromonas gingivalis bacteria causes an increase in proteolytic activity on the expression and activation of MMP-7, which causes loss of attachment. Efficacious plant extracts, namely irrigated curcumin 1%, have an anti-inflammatory effect. This research is independent research using experimental white rats (Rattus norvegicus) male strain Wistar, 20 weeks old, with a body weight of 325-350 grams. This research is to see the levels of MMP-7 with the ELISA method. Forty-eight periodontitis model rats were divided into two groups. Twenty-four periodontitis model rats as the control group were exposed to Porphyromonas gingivalis, and another twenty-four periodontitis model rats as the treatment group were exposed to Porphyromonas gingivalis and 1% curcumin irrigation. Each group was observed at 24 hours, on days-7 and days-14. The results showed that MMP-7 levels in the treatment group were significantly lower than in the control group on on days-7 and days-14. In the treatment group, MMP-7 levels at 24 hours were significantly lower than on the on days-7 and days-14. In the treatment group, MMP-7 levels did not experience a significant difference between the on days-7 and days-14. The results of this study indicate that 1% curcumin can reduce MMP-7 levels in cases of chronic periodontitis due to Porphyromonas gingivalis bacteria.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"393 1-3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140502395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory activity of Codium elongatum on Carrageenan-induced paw edema in Wistar male rats","authors":"Himanshi Himanshi, Pushpraj S Gupta","doi":"10.52711/0974-360x.2024.00031","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00031","url":null,"abstract":"Inflammation is the physiological defence system of the body to initiate the healing mechanism for tissue and eliminate injurious stimuli. In the present study, the anti-inflammatory activity of Codium elongatum was evaluated. Water extract (250 mg/kg, b.w.) and isolated polysaccharide (500 mg/kg, b.w.) are investigated in the carrageenan-induced rat paw edema model using phenylbutazone as a standard drug. Both water extract and isolated polysaccharide showed significant anti-inflammatory activity by blocking the cyclooxygenase 2 pathway. This is the first report to demonstrate the in-vivo anti-inflammatory activity of Codium elongatum.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"74 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140503281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}