Research Journal of Pharmacy and Technology最新文献

{"title":"Multitarget Ensemble Docking of Potent Anticancer and Antioxidant Active Compounds from the Acacia auriculiformis and Acacia crassicarpa","authors":"Yanico Hadi Prayogo, Setyanto Tri Wahyudi, Irmanida Batubara, Rita Kartika Sari, W. Syafii","doi":"10.52711/0974-360x.2024.00110","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00110","url":null,"abstract":"Bioactive chemicals derived from Acacia auriculiformis and A. crassicarpa have the potential to be developed as sources of anti-cancer raw materials and antioxidants, given that these plants are fast-growing species with medicinal capability. The in silico method was successful in identifying these bioactive chemicals for the preliminary study. Using an in silico approach, this work aimed to identify the most potent compounds as inhibitors of six cancer and stress oxidative therapy-targeted proteins from these two distinct Acacia species. Seventeen out of the 37 compounds examined exhibited low affinity and satisfied the drug-likeness criterion. Five active chemicals were identified by redocking analysis: auriculoside, 3-(3,4-dihydroxybenzyl)-7-hydroxychroman-4-one, kaempferol 7-O-glucoside, quercetin 7-O-glucoside, and keto-teracacidin. According to simulations of molecular dynamics, molecular motion occurs with a root mean square deviation of less than four and generates at least eleven receptor conformations for 0 to 100 ns. Auriculoside showed the lowest average binding energy against four receptors in colorectal and breast cancer, as determined by ensemble docking, followed by 3-(3,4-dihydroxybenzyl)-7-hydroxychroman-4-one, quercetin 7-O-glucoside, and kaempferol 7-O-glucoside. Auriculoside shown multitarget inhibitory effect against colorectal cancer by inhibiting cyclin dependent kinase-6 and breast cancer by inhibiting epidermal growth factor receptor and mammalian target of rapamycin. Auriculoside has the powerful ability to inhibit glycogen synthase kinase-3 beta, hence regulating oxidative stress. Kaempferol 7-O-glucoside and quercetin 7-O-glucoside also exhibited a possible single protein targeting method against breast cancer. These findings are essential for future research targeted at developing these plants as potent natural therapeutic raw materials and for isolating or synthesizing compounds with anticancer and oxidative stress-regulating antioxidant properties.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"83 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140448627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of the Quantitative Determination Method of the active substance in Angiolin eye drops by spectrophotometry","authors":"Liudmyla I. Kucherenko, Rima R. Akopian, Оlena О. Portna, Natalia V. Derevianko, Dmytro Yu. Skoryna","doi":"10.52711/0974-360x.2024.00117","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00117","url":null,"abstract":"Today, cataracts are the main cause of vision loss. Therefore, the creation of new ophthalmic medicines (drugs), namely eye drops, based on the active substance angiolin ((S)-2,6-diaminohexanoic acid 3-methyl-1,2,4-triazolyl-5-thioacetate) is an urgent task. Previously, we developed a method of standardization of the active substance – by the spectrophotometry. According to the requirements of the SPhU, the next step was the validation of the developed methods. That became the purpose of our work. Validation of the method of quantitative determination of active substances was carried out according to the following indicators: specificity, linearity, range of application, accuracy, correctness and robustness. In the course of the conducted studies, it was established that the method is characterized by sufficient convergence and correctness, as the criterion of insignificance of the systematic error of the method is fulfilled. The systematic error of the method satisfies the requirements of statistical and practical insignificance. The high value of the correlation coefficient r = 1.0000 and 0.99994 satisfies the requirements of the acceptance criterion (r = 0.9998) and confirms the linearity of the dependence between the amount of Angiolin taken and found in the range from 80% to 120%, according to its nominal content in the drug. The requirements for the linear dependence parameters (a, SD0/b, r) of the Angiolin determination method are met in the entire concentration range from 80% to 120% of the nominal value. Thus, the developed method of standardization of active substances in Angiolin eye drops is valid and can be introduced into the project of QCM (quality control methods).","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"191 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140448743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenolic Content and Antioxidant Activity of Hydroethanolic and Aqueous Extracts of the Inflorescences of Allium sphaerocephalon L.","authors":"Chahrazed Kaoudoune, Fatima Benchikh, Cherif Abdennour, Hassiba Benabdallah, Chawki Ben Souici, Ismahane Derafa, Walid Mamache, Smain Amira","doi":"10.52711/0974-360x.2024.00140","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00140","url":null,"abstract":"The objective of this effort is to quantify the amounts of phenolics and the in vitro antioxidant capacities of Allium sphaerocephalon L. flowers’ hydroethanolic (EOH) and aqueous (AQE) extracts. The two extracts' total phenolic (TPC) and flavonoid content (TFC) were determined spectrophotometrically. Results showed the extraction yield obtained by EOH and AQE were 18.30% and 11.80%, respectively. The EOH gave higher amounts of TPC (12.73±0.91µg GAE/mg DW) and TFC (7.67±0.24µg QE/mg DW) than AQE (TPC = 9.71±0.53µg GAE/mg DW and TFC= 5.68±0.90µg QE/ mg DW). Five techniques were used to train antioxidant activity. The EOH extract had high activity in DPPH radical scavenging capacities and β-carotene bleaching assay, while the AQE extract had high activity in metal chelating ions and reducing power assay. However, both extracts had no different significance in cupric-reducing capacity. A moderate quantity of flavonoids and polyphenols, as well as a satisfactory level of antioxidant activity, were observed in both preparations derived from the flowers of A. sphaerocephalon L., where this activity is not all fully related to the amount of phenolic compounds.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"129 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140449071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytoconstituents of Leaves and Roots Ethanolic Extract of Talinum paniculatum and Their Biological Activities","authors":"Susilo Susilo, Fadita Nurul Aini, Etin Diah Permanasari","doi":"10.52711/0974-360x.2024.00105","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00105","url":null,"abstract":"Javanese ginseng (Talinum paniculatum) is known to the people of Indonesia to have properties in medicine and food. People's culture generally uses leaves and roots that, until now, constituent information has yet to bereported. Therefore, variations of phytoconstituents in the leaves and roots of T. paniculatum were investigated using Gas Chromatography-Mass Spectrometry (GC-MS). The roots of T. paniculatum contain 17 active compounds dominated by N,N'-Dibutyl-N,N'-dimethylurea. The leaf has only 16 active compounds, but Python compounds from the diterpenoids class dominate it. In general, T. paniculatum contains a variety of steroid class compounds, namely Stigmasterol; Stigmast-5-en-3-ol; and 9,19-Cycloergost-24(28)-en-3-ol, 4,14-dimethyl-, acetate, (3,β., 4.α., 5.α.) - in the leaves, and stigmasterol and .γ.Sitosterol in the root. Python is known to have high efficacy as an antimicrobial, antifungal, antibacterial, antiparasitic, antimutagenic, and antioxidant. At the same time, steroid compounds are anti-cancer, antioxidant, anti-tumor, antidiabetic, and anti-inflammatory agents. The results of identifying compounds in T. paniculatum can be used as a reference in optimizing the use of T. paniculatumin the future.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"217 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140446009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Dual effect of Citric acid on Preparation and Preservation of Guava Jelly via Physicochemical analysis","authors":"Gul-e-Saba Chaudhry, Abdul Matin, Isfaq Wahid Bin Rahim, Mizanur Rahman, Yeong Yik Sung, Tengku Muhammad Tengku Sifzizul","doi":"10.52711/0974-360x.2024.00080","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00080","url":null,"abstract":"This study was conducted to examined the effects of citric acid concentration on the processing and storage of guava jelly. Six different types of jelly were prepared with 0.15%, 0.2% and 0.25% pectin and with 1.1%, 1.15% and 1.2% citric acid. No preservatives were added during the processing of Jelly. The analysis showed Jelly contains a mean moisture content of 26.5%, acidity of 0.28%, total sugar of 39.1%, total soluble solid of 68.5%, reducing sugar 30.13%, and nonreducing sugar 8.63%. The citric acid had no effect on processed product quality. There is no mentionablechanges were found during 90 days of storage at room temperature (25oC to 30oC). However, moisture content, total soluble solid, acidity, and the reducing sugar of the products were slightly increased.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"685 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140446294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Method development and validation for the simultaneous estimation of Doultegravir and Rilpivirine related impurities (Rilpivirine Z Isomer and Dolutegravir hydroxy impurity) using RP HPLC","authors":"Varada Soujanya, Revu Baby Nalanda","doi":"10.52711/0974-360x.2024.00093","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00093","url":null,"abstract":"The combination of Doultegravir and Rilpivirine is used to treat human immunodeficiency (HIV) virus. A new stability indicating RP-HPLC method has been proposed for the quantification of Doultegravir and Rilpivirine along with its impurities Rilpivirine Z Isomer and Doultegravir hydroxy impurity using Water HPLC System (PDA detector) and auto sampler integrated with Empower 2 Software with Inertsil (250 × 4.6 mm, 5 μ) C18 column (PDA detector) was used for the present study. A mixture of 0.01N phosphate buffer and acetonitrile (50: 50, v/v) (pH adjusted to 4.8 with TEA and ortho phosphoric acid) was used as mobile phase for the chromatographic study (Flow rate: 1.0 ml/min; Injection volume: 10 μl; Detection wavelength: 257 nm) with run time 12 mins. Stress degradation studies were performed and the method was validated as per ICH guidelines. The developed method was found to be precise, specific, accurate, linear, stable and robust for the quantification of Dolutegravir and Rilpivirine along with its impurities Rilpivirine Z Isomer and Dolutegravir hydroxy impurity and its bulk drug formulation. The developed method can be applied successfully to quality control and for other analytical purposes.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"459 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140446902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of the Composition and Technology of Obtaining a Dental film with Portulaca oleracea extract","authors":"Meruyert I. Tleubayeva, Shynar Tukezhan, Gaukhar I. Yeshenkulova, Aibala Daurenkyzy, Raisa М. Abdullabekova","doi":"10.52711/0974-360x.2024.00096","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00096","url":null,"abstract":"According to the concept of the World Health Organization (WHO), oral health is the main indicator of the overall level of health and well-being, quality of life. Currently, improving oral health, treatment and prevention of chronic diseases of the mucous membrane are relevant. The creation of new dosage forms is an urgent problem, since existing dosage forms have both advantages and disadvantages. Dental film based on biologically active compounds of plant materials is a promising dosage form. The article presents the results of a study on the selection of ingredients for the rational composition of dental films based on carbon dioxide extract of Portulacaoleracea. Model formulations with various excipients have been developed for the selection of ingredients. In order to select the rational composition, a study was conducted on such characteristics as description, average mass, deviation from average mass, pH value, solubility, and the results of the study were described. The rational composition of the dental film with the conditional name \"DentaPort\" was established: the active substance is carbon dioxide extract of Portulaca oleracea, the formative substance is sodium alginate, a moisture-saving substance and a plasticizer is glycerin, powdered sugar is a corrigent, purified water is a solvent. The excipients were selected taking into account their compatibility with each other and the extract of Portulacaoleracea.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"426 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140448044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethnobotanical Study of Medicinal plants used for Traditional Diarrhea Treatment in North-East Algeria","authors":"Saliha Torche, Katiba Beroual, Mohamed Zaouani, Sofiane Boujellaba","doi":"10.52711/0974-360x.2024.00126","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00126","url":null,"abstract":"The objective of this study is to aid in the identification and documentation of medicinal plants that are utilized in traditional local pharmacopeia for the treatment of diarrhea in four provinces located in the northeast region of Algeria. The ethnobotanical survey was conducted during 2019-2020. All users of medicinal plants in those regions were interviewed using a guide questionnaire. Stratified sampling was used to collect information on medicinal plants. Furthermore, the study established the relative importance of the species and estimated the informant consensus factor (ICF) for the medicinal plants included in research. The study has found a total of 52 species that are commonly employed in the treatment of diarrhea in four specific provinces of Algeria. These species are categorized into 26 families and 22 genera. The most frequently quoted plants (over 10 quotations) were Artemisia herba alba Asso. (52 quotations) with the highest (FC = 43.33%); Punica granatum L. (50 quotations, FC = 41.67%); Origanum vulgaire L. (32 quotations, FC = 26.67%); Juniperus phoenicea L. (29 quotations, FC = 24.17%), and Ceratonia siliqua L. (19 quotations, FC = 15.83%). The most common plant families were Lamiaceae and Rosaceae (10 and 6 plants, respectively). Species morphological type analyses show that herbs were most represented (46.16%). For different organs used, leaves and fruits were the most used (27.15% and 26.35%, respectively). Although the infusion (55.81%) constituted the prevailing approach to drug preparation, the results showed that many kinds of plants can be used to treat diarrhea in Algeria. The study has enhanced the collection of medicinal plants with anti-diarrheal properties and has contributed to the advancement of knowledge in traditional medicine while also preserving local indigenous knowledge.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"88 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140448595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammation and Anti-tyrosinase effect of Robusta coffee BP-42 extract gel on clinical appearance after skin grafting in long evans rats","authors":"Ulfa Elfiah, David Sontani Perdanakusuma, Iswinarno Doso Saputro, Misnawi Misnawi","doi":"10.52711/0974-360x.2024.00099","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00099","url":null,"abstract":"This study compares the anti-hyperpigmentation activity of kojic acid and Robusta BP-42 coffee bean extract on skin-grafted rats. Kojic acid is one of the standards for reducing pigmentation on the skin. But, kojic acid usage presents side effects for some patients, such as skin irritation, redness, and itching. Robusta BP-42 is one of the best robusta coffee strains found in Indonesia. The Indonesian Coffee and Cocoa Research Institute, Jember Regency, East Java, provides this coffee. BP-42 coffee has the highest quality grain and organoleptic score compared to other strains. Three groups of grafted skin on long Evans rats were treated with 2% kojic acid gel (positive control), 5% robusta BP-42 coffee extract gel, and placebo gel (negative control). Observation occurred for 21 days. The photos of grafted skin were taken on days 0, 4, 7, 11, 18 and 21. Skin pigmentation was measured using ImageJ software. Histological examination was performed on days 7, 14, and 21 for all treatment groups. These histological preparations were stained with Masson Fontana. The result showed that Robusta BP-42 coffee extract gel showed a similar clinical and histological measurement to positive control. Robusta BP-42 coffee can be a new candidate to treat hyperpigmentation after skin graft surgery.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"39 31","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140449245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationships between Ultrasonographic Placental Thickness in the Third Trimester and Foetal Outcomes","authors":"Digvijay Kadam, Yamini Patil, R. Patange, S. Patil, Shiju Sebastian, R. Jarag","doi":"10.52711/0974-360x.2024.00116","DOIUrl":"https://doi.org/10.52711/0974-360x.2024.00116","url":null,"abstract":"Poor neonatal outcomes, including low birth weight (LBW), poor APGAR scores, more NICU hospitalizations, and a higher chance to develop Pre-Eclampsia, IUGR, and Oligo Hydramnios, are all linked to thin placental thickness. While both thin and thick placentae are connected to a greater prevalence of C-sections, thick placentae are linked with a greater possibility of developing GDM and an increase in NICU hospitalizations. Objective of this research was to investigate the association between placental thickness as measured by ultrasonography in the third trimester and foetal outcome, including the relationship between placental histopathology and placental thickness. investigate the link among placental thickness, foetal outcome, and placental histology. Most newborns had fibrinoid necrosis and calcifications. Babies with Macrosomia and IUGR, respectively, were more likely to develop Syncytial knots and thickening of the vessel wall. Patients with normal placenta thickness at 36 weeks' gestation experienced fewer difficulties than those with thin or thick placentas at the same time. The study emphasizes the value of evaluating placental thickness using ultrasound in the third trimester to detect high-risk pregnancies. The study also shows that aberrant foetal and neonatal events are linked to certain placental histological characteristics, like artery wall thickening and infarctions.","PeriodicalId":21141,"journal":{"name":"Research Journal of Pharmacy and Technology","volume":"92 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140449325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}