Multi-Step Maceration Extraction and Pharmacological Evaluation of Abelmoschus manihot (L.) Medik

Q3 Pharmacology, Toxicology and Pharmaceutics

Research Journal of Pharmacy and Technology Pub Date : 2024-01-19 DOI:10.52711/0974-360x.2024.00061

Ardina Purnama Tirta, Imas Solihat, Achmad Nandang Roziafanto, Nurhasanah Nurhasanah, Ratnawati L Djanis, Inda Mapiliandari

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Abstract

A study has been conducted on the phytochemical profile and pharmacological activity of Gedi (Abelmoschus manihot (L.) Medik) using a multistage maceration method. The results of the phytochemical screening test showed that the crude extract of Gedi leaves gave a positive reaction to the presence of alkaloids, tannins, phenols, flavonoids, unsaturated steroids and saponins. The total phenolic analysis showed that the total phenolic concentrations of Gedi leaf extracts in methanol, ethyl acetate, and hexane extracts were 1.70, 1.44, and 0.85mg GAE/g extract, respectively. The results of the antioxidant activity assays obtained an IC50 value for methanol, ethyl acetate, and hexane extracts of Gedi leaves, which were 40.77, 155.50, and 25.84mg/L, respectively. The antimicrobial activity results showed that all three Gedi leaf extracts had activity in inhibiting microbial growth. The inhibitory values for methanol, ethyl acetate, and hexane extracts of Gedi leaves were 7.0, 13.5, and 9.5mm, respectively, for Escherichia coli, and 21.5, 15.0, and 13.0mm, respectively, for Bacillus. The toxicity test results showed that the LC50 values of Gedi leaf extracts for methanol, ethyl acetate, and hexane extracts were 500 ppm, 736ppm, and 2249ppm, respectively.

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Abelmoschus manihot (L.) Medik 的多步浸渍萃取和药理评估

采用多级浸渍法对 Gedi（Abelmoschus manihot (L.) Medik）的植物化学成分和药理活性进行了研究。植物化学筛选试验结果表明，Gedi 叶片的粗提取物中生物碱、单宁、酚类、黄酮类、不饱和类固醇和皂苷的含量呈阳性反应。总酚分析表明，甲醇、乙酸乙酯和正己烷提取物中的总酚浓度分别为 1.70、1.44 和 0.85 毫克 GAE/克。抗氧化活性测定结果表明，Gedi 叶甲醇、乙酸乙酯和己烷提取物的 IC50 值分别为 40.77、155.50 和 25.84 毫克/升。抗菌活性结果表明，三种 Gedi 叶提取物都具有抑制微生物生长的活性。甲醇、乙酸乙酯和正己烷提取物对大肠杆菌的抑制值分别为 7.0、13.5 和 9.5 毫米，对芽孢杆菌的抑制值分别为 21.5、15.0 和 13.0 毫米。毒性试验结果表明，Gedi 叶提取物的甲醇、乙酸乙酯和正己烷提取物的半数致死浓度分别为 500 ppm、736ppm 和 2249ppm。

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来源期刊

Research Journal of Pharmacy and Technology Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal, devoted to pharmaceutical sciences. The aim of RJPT is to increase the impact of pharmaceutical research both in academia and industry, with strong emphasis on quality and originality. RJPT publishes Original Research Articles, Short Communications, Review Articles in all areas of pharmaceutical sciences from the discovery of a drug up to clinical evaluation. Topics covered are: Pharmaceutics and Pharmacokinetics; Pharmaceutical chemistry including medicinal and analytical chemistry; Pharmacognosy including herbal products standardization and Phytochemistry; Pharmacology: Allied sciences including drug regulatory affairs, Pharmaceutical Marketing, Pharmaceutical Microbiology, Pharmaceutical biochemistry, Pharmaceutical Education and Hospital Pharmacy.