Research最新文献

{"title":"Light-Programmable g-C₃N₄ Microrobots with Negative Photogravitaxis for Photocatalytic Antibiotic Degradation.","authors":"Yunhuan Yuan, Xianghua Wu, Bindu Kalleshappa, Martin Pumera","doi":"10.34133/research.0565","DOIUrl":"10.34133/research.0565","url":null,"abstract":"<p><p>Microrobots enhance contact with pollutants through their movement and flow-induced mixing, substantially improving wastewater treatment efficiency beyond traditional diffusion-limited methods. g-C₃N₄ is an affordable and environmentally friendly photocatalyst that has been extensively researched in various fields such as biomedicine and environmental remediation. However, compared to other photocatalytic materials like TiO₂ and ZnO, which are widely used in the fabrication of micro- and nanorobots, research on g-C₃N₄ for these applications is still in its early stages. This work presents microrobots entirely based on g-C₃N₄ microtubes, which can initiate autonomous movement when exposed to ultraviolet and visible light. We observed distinct motion behaviors of the microrobots under light irradiation of different wavelengths. Specifically, under ultraviolet light, the microrobots exhibit negative photogravitaxis, while under visible light, they demonstrate a combination of 3-dimensional motion and 2-dimensional motion. Therefore, the wavelength of the light can be used for programming the motion style of the microrobots and subsequently their application. We show that the microrobots can effectively degrade the antibiotic tetracycline, displaying their potential for antibiotic removal. This exploration of autonomous motion behaviors under different wavelength conditions helps to expand research on g-C₃N₄-based microrobots and their potential for environmental remediation.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0565"},"PeriodicalIF":11.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11772662/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesenchymal Stem Cells Carrying Viral Fusogenic Protein p14 to Treat Solid Tumors by Inducing Cell-Cell Fusion and Immune Activation.","authors":"Yao Wang, Xunlei Pang, Ruirui Li, Jiuzhou Chen, Chen Wen, Huihuang Zhu, Tingyu Long, Jianjie Li, Lijun Zheng, Youcai Deng, Junnian Zheng, Bo Xu","doi":"10.34133/research.0594","DOIUrl":"10.34133/research.0594","url":null,"abstract":"<p><p><b>Background:</b> Chimeric antigen receptor (CAR)-based immune cell therapies attack neighboring cancer cells after receptor recognition but are unable to directly affect distant tumor cells. This limitation may contribute to their inefficiency in treating solid tumors, given the restricted intratumoral infiltration and immunosuppressive tumor microenvironment. Therefore, cell-cell fusion as a cell-killing mechanism might develop a novel cytotherapy aimed at improving the efficacy against solid tumors. <b>Methods:</b> We constructed a fusogenic protein, fusion-associated small transmembrane (FAST) p14 of reptilian reovirus, into cancer cells and mesenchymal stem cells (MSCs), which cocultured with various colon cancer cells and melenoma cells to validate its ability to induce cell fusion and syncytia formation. RNA sequencing, quantitative reverse transcription polymerase chain reaction, and Western blot were performed to elucidate the mechanism of syncytia death. Cell viability assay was employed to assess the killing effects of MSCs carrying the p14 protein (MSCs-p14), which was also identified in the subcutaneous tumor models. Subsequently, the Tet-On system was introduced to enhance the controllability and safety of therapy. <b>Results:</b> Cancer cells incorporated with fusogenic protein p14 FAST from reovirus fused together to form syncytia and subsequently died through apoptosis and pyroptosis. MSCs-p14 cocultured with different cancer cells and effienctly induced cancer cell fusion and caused widespread cancer cell death in vitro. In mouse tumor models, mMSCs-p14 treatment markedly suppressed tumor growth and also enhanced the activity of natural killer cells and macrophages. Controllability and safety of MSCs-p14 therapy were further improved by introducing the tetracycline-controlled transcriptional system. <b>Conclusion:</b> MSC-based cytotherapy carrying viral fusogenic protein in this study kills cancer cells by inducing cell-cell fusion. It has demonstrated definite efficacy in treating solid tumors and is worth considering for clinical development.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0594"},"PeriodicalIF":11.0,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11770199/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143053456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to \"Sleep Promotion by 3-Hydroxy-4-iminobutyric Acid in Walnut <i>Diaphragma Juglandis Fructus</i>\".","authors":"Jian Ji, Yongli Ye, Lina Sheng, Jiadi Sun, Qianqian Hong, Chang Liu, Jun Ding, Shuxiang Geng, Deping Xu, Yinzhi Zhang, Xiulan Sun","doi":"10.34133/research.0585","DOIUrl":"10.34133/research.0585","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.34133/research.0216.].</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0585"},"PeriodicalIF":11.0,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11770198/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143053453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorescent Particles Based on Aggregation-Induced Emission for Optical Diagnostics of the Central Nervous System.","authors":"Shan Liu, Jinkuan Liu, Xue Li, Xiaoxin Du, Cheng Yin, Yong Luo, Chenzhong Li","doi":"10.34133/research.0564","DOIUrl":"10.34133/research.0564","url":null,"abstract":"<p><p>In 2001, Tang's team discovered a unique type of luminogens with substantial enhanced fluorescence upon aggregation and introduced the concept of \"aggregation-induced emission (AIE)\". Unlike conventional fluorescent materials, AIE luminogens (AIEgens) emit weak or no fluorescence in solution but become highly fluorescent in aggregated or solid states, due to a mechanism known as restriction of intramolecular motions (RIM). Initially considered a purely inorganic chemical phenomenon, AIE was later applied in biomedicine to improve the sensitivity of immunoassays. Subsequently, AIE has been extensively explored in various biomedical applications, especially in cell imaging. Early studies achieved nonspecific cell imaging using nontargeted AIEgens, and later, specific cellular imaging was realized through the design of targeted AIEgens. These advancements have enabled the visualization of various biomacromolecules and intracellular organelles, providing valuable insights into cellular microenvironments and statuses. Neurological disorders affect over 3 billion people worldwide, highlighting the urgent need for advanced diagnostic and therapeutic tools. AIEgens offer promising opportunities for imaging the central nervous system (CNS), including nerve cells, neural tissues, and blood vessels. This review focuses on the application of AIEgens in CNS imaging, exploring their roles in the diagnosis of various neurological diseases. We will discuss the evolution and conclude with an outlook on the future challenges and opportunities for AIEgens in clinical diagnostics and therapeutics of CNS disorders.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0564"},"PeriodicalIF":11.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11757665/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crosstalk between Gut Microbiota and Cancer Immunotherapy: Present Investigations and Future Perspective.","authors":"Yuhui Tang, Qiaoting Cai, Zhi Tian, Wenkuan Chen, Hailin Tang","doi":"10.34133/research.0600","DOIUrl":"10.34133/research.0600","url":null,"abstract":"<p><p>Gut microbiota is crucial for protecting the homeostasis of immune locally and systemically, and its dysbiosis is essentially correlated to tumorigenesis, cancer progression, and refractoriness to cancer treatments, including the novel immunotherapy. Increasing evidence unravel the intricate role of gut microbiota in reshaping tumor microenvironment and affecting the efficacy and toxicities of immunotherapy, which shed more light on the future applications of gut microbiota in efficacious biomarker and combination treatment of immunotherapy. To better grasp the underlying crosstalk between gut microbiota and immunotherapy, more experimental and clinical trials are indispensable for the customized gut microbiota-based treatments in cancer patients undergoing immunotherapy.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0600"},"PeriodicalIF":11.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesis of Lysosomally Escaped Apoptotic Bodies Inhibits Inflammasome Synthesis in Macrophages.","authors":"Jiayi Mao, Wenzheng Xia, Yanglin Wu, Minxiong Li, Yun Zhao, Peisong Zhai, Yuguang Zhang, Tao Zan, Wenguo Cui, Xiaoming Sun","doi":"10.34133/research.0581","DOIUrl":"10.34133/research.0581","url":null,"abstract":"<p><p>Hyperglycemia and bacterial colonization in diabetic wounds aberrantly activate Nod-like receptor protein 3 (NLRP3) in macrophages, resulting in extensive inflammatory infiltration and impaired wound healing. Targeted suppression of the NLRP3 inflammasome shows promise in reducing macrophage inflammatory disruptions. However, challenges such as drug off-target effects and degradation via lysosomal capture remain during treatment. In this study, engineered apoptotic bodies (BHB-dABs) derived from adipose stem cells loaded with β-hydroxybutyric acid (BHB) were synthesized via biosynthesis. These vesicles target M1-type macrophages, which highly express the folic acid receptor in the inflammatory microenvironment, and facilitate lysosomal escape through 1,2-distearoyl-<i>sn</i>-propyltriyl-3-phosphatidylethanolamine-polyethylene glycol functionalization, which may enhance the efficacy of NLRP3 inhibition for managing diabetic wounds. In vitro studies demonstrated the biocompatibility of BHB-dABs, their selective targeting of M1-type macrophages, and their ability to release BHB within the inflammatory microenvironment via folic acid and folic acid receptor signaling. These nanovesicles exhibited lysosomal escape, anti-inflammatory, mitochondrial protection, and endothelial cell vascularization properties. In vivo experiments demonstrated that BHB-dABs enhance the recovery of diabetic wound inflammation and angiogenesis, accelerating wound healing. These functionalized apoptotic bodies efficiently deliver NLRP3 inflammasome inhibitors using a dual strategy of targeting macrophages and promoting lysosomal escape. This approach represents a novel therapeutic strategy for effectively treating chronic diabetic wounds.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0581"},"PeriodicalIF":11.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754539/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Scar Effects of Micropatterned Hydrogel after Glaucoma Drainage Device Implantation.","authors":"Yiling Han, Qiangwang Geng, Aimeng Dong, Menglu Jiang, Jingyi Ma, Wulian Song, Pan Fan, Yuanyuan Li, Jiawen Gao, Fenghua Zhang, Jinsong Leng, Huiping Yuan","doi":"10.34133/research.0561","DOIUrl":"10.34133/research.0561","url":null,"abstract":"<p><p>Excessive fibrosis is the primary factor for the failure of glaucoma drainage device (GDD) implantation. Thus, strategies to suppress scar formation in GDD implantation are crucial. Although it is known that in implanted medical devices, microscale modification of the implant surface can modulate cell behavior and reduce the incidence of fibrosis, in the field of ophthalmic implants, especially the modification and effects of hydrogel micropatterns have rarely been reported. Here, we designed the patterned gelatin/acrylamide double network hydrogel and developed an innovative GDD with micropattern to suppress inflammatory and fibroblast activation after GDD implantation. Pattern topography suppressed F-actin expression and mitigated actin-dependent nuclear migration of myocardin-related transcription factor A (MRTF-A) during the proliferative phase after GDD implantation. Ultimately, the expression of α-smooth muscle actin (α-SMA), a key fibrosis-related gene product, was suppressed. Moreover, the modified GDD effectively controlled intraocular pressure (IOP), mitigated fibrous formation, and remodeled extracellular matrix (ECM) collagen distribution in vivo. Therefore, the novel GDD with surface patterning interventions provides a promising strategy to inhibit scar formation after GDD implantation and raise the efficacy of GDD implantation.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0561"},"PeriodicalIF":11.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751202/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altered Atlas of Exercise-Responsive MicroRNAs Revealing miR-29a-3p Attacks Armored and Cold Tumors and Boosts Anti-B7-H3 Therapy.","authors":"Jie Mei, Zhiwen Luo, Yun Cai, Renwen Wan, Zhiwen Qian, Jiahui Chu, Yaying Sun, Yuxin Shi, Ying Jiang, Yan Zhang, Yongmei Yin, Shiyi Chen","doi":"10.34133/research.0590","DOIUrl":"10.34133/research.0590","url":null,"abstract":"<p><p>Increasing evidence has shown that physical exercise remarkably inhibits oncogenesis and progression of numerous cancers and exercise-responsive microRNAs (miRNAs) exert a marked role in exercise-mediated tumor suppression. In this research, expression and prognostic values of exercise-responsive miRNAs were examined in breast cancer (BRCA) and further pan-cancer types. In addition, multiple independent public and in-house cohorts, in vitro assays involving multiple, macrophages, fibroblasts, and tumor cells, and in vivo models were utilized to uncover the tumor-suppressive roles of miR-29a-3p in cancers. Here, we reported that miR-29a-3p was the exercise-responsive miRNA, which was lowly expressed in tumor tissues and associated with unfavorable prognosis in BRCA. Mechanistically, miR-29a-3p targeted macrophages, fibroblasts, and tumor cells to down-regulate B7 homolog 3 (B7-H3) expression. Single-cell RNA sequencing (scRNA-seq) and cytometry by time-of-flight (CyTOF) demonstrated that miR-29a-3p attacked the armored and cold tumors, thereby shaping an immuno-hot tumor microenvironment (TME). Translationally, liposomes were developed and loaded with miR-29a-3p (lipo@miR-29a-3p), and lipo@miR-29a-3p exhibited promising antitumor effects in a mouse model with great biocompatibility. In conclusion, we uncovered that miR-29a-3p is a critical exercise-responsive miRNA, which attacked armored and cold tumors by inhibiting B7-H3 expression. Thus, miR-29a-3p restoration could be an alternative strategy for antitumor therapy.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0590"},"PeriodicalIF":11.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Networked Intelligent Elderly Care Model Based on Nursing Robots to Achieve Healthy Aging.","authors":"Wenting Ji, Ruzhen Luo, Yumei Sun, Maiping Yang, Yueheng Liu, Hua Chen, Dongmei Lin, Ziyi Su, Guangming Tao, Diansheng Chen, Hongyu Sun","doi":"10.34133/research.0592","DOIUrl":"10.34133/research.0592","url":null,"abstract":"<p><p>As global populations become increasingly aged, existing elderly care models are proving insufficient. The development and application of nursing robots have shown potential in addressing the challenges of elder care in aging societies. This perspective outlines current state and potential applications of nursing robots in promoting healthy aging. Given this background, a networked intelligent elderly care model for nursing robots, which integrates technologies such as big data, artificial intelligence, the Internet of Things, and nursing robotics, is proposed. This model would synergistically combine elderly health monitoring, capability assessment, and intelligent allocation functions to revolutionize global elderly care practices and promote healthy aging.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0592"},"PeriodicalIF":11.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Terahertz Science and Technology in Astronomy, Telecommunications, and Biophysics.","authors":"Jing Li, Xianjin Deng, Yangmei Li, Jie Hu, Wei Miao, Changxing Lin, Jun Jiang, Shengcai Shi","doi":"10.34133/research.0586","DOIUrl":"10.34133/research.0586","url":null,"abstract":"<p><p>This paper reviews recent developments and key advances in terahertz (THz) science, technology, and applications, focusing on 3 core areas: astronomy, telecommunications, and biophysics. In THz astronomy, it highlights major discoveries and ongoing projects, emphasizing the role of advanced superconducting technologies, including superconductor-insulator-superconductor (SIS) mixers, hot electron boundedness spectroscopy (HEB), transition-edge sensors (TESs), and kinetic inductance detectors (KIDs), while exploring prospects in the field. For THz telecommunication, it discusses progress in solid-state sources, new communication technologies operating within the THz band, and diverse modulation methods that enhance transmission capabilities. In THz biophysics, the focus shifts to the physical modulation of THz waves and their impact across biological systems, from whole organisms to cellular and molecular levels, emphasizing nonthermal effects and fundamental mechanisms. This review concludes with an analysis of the challenges and perspectives shaping the future of THz technology.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0586"},"PeriodicalIF":11.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}