Research最新文献

Crosstalk between Gut Microbiota and Cancer Immunotherapy: Present Investigations and Future Perspective.

IF 11 1区综合性期刊

Research Pub Date : 2025-01-23 eCollection Date: 2025-01-01 DOI: 10.34133/research.0600

Yuhui Tang, Qiaoting Cai, Zhi Tian, Wenkuan Chen, Hailin Tang

引用次数: 0

Biosynthesis of Lysosomally Escaped Apoptotic Bodies Inhibits Inflammasome Synthesis in Macrophages.

IF 11 1区综合性期刊

Research Pub Date : 2025-01-23 eCollection Date: 2025-01-01 DOI: 10.34133/research.0581

Jiayi Mao, Wenzheng Xia, Yanglin Wu, Minxiong Li, Yun Zhao, Peisong Zhai, Yuguang Zhang, Tao Zan, Wenguo Cui, Xiaoming Sun

{"title":"Biosynthesis of Lysosomally Escaped Apoptotic Bodies Inhibits Inflammasome Synthesis in Macrophages.","authors":"Jiayi Mao, Wenzheng Xia, Yanglin Wu, Minxiong Li, Yun Zhao, Peisong Zhai, Yuguang Zhang, Tao Zan, Wenguo Cui, Xiaoming Sun","doi":"10.34133/research.0581","DOIUrl":"https://doi.org/10.34133/research.0581","url":null,"abstract":"Hyperglycemia and bacterial colonization in diabetic wounds aberrantly activate Nod-like receptor protein 3 (NLRP3) in macrophages, resulting in extensive inflammatory infiltration and impaired wound healing. Targeted suppression of the NLRP3 inflammasome shows promise in reducing macrophage inflammatory disruptions. However, challenges such as drug off-target effects and degradation via lysosomal capture remain during treatment. In this study, engineered apoptotic bodies (BHB-dABs) derived from adipose stem cells loaded with β-hydroxybutyric acid (BHB) were synthesized via biosynthesis. These vesicles target M1-type macrophages, which highly express the folic acid receptor in the inflammatory microenvironment, and facilitate lysosomal escape through 1,2-distearoyl-sn-propyltriyl-3-phosphatidylethanolamine-polyethylene glycol functionalization, which may enhance the efficacy of NLRP3 inhibition for managing diabetic wounds. In vitro studies demonstrated the biocompatibility of BHB-dABs, their selective targeting of M1-type macrophages, and their ability to release BHB within the inflammatory microenvironment via folic acid and folic acid receptor signaling. These nanovesicles exhibited lysosomal escape, anti-inflammatory, mitochondrial protection, and endothelial cell vascularization properties. In vivo experiments demonstrated that BHB-dABs enhance the recovery of diabetic wound inflammation and angiogenesis, accelerating wound healing. These functionalized apoptotic bodies efficiently deliver NLRP3 inflammasome inhibitors using a dual strategy of targeting macrophages and promoting lysosomal escape. This approach represents a novel therapeutic strategy for effectively treating chronic diabetic wounds.","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0581"},"PeriodicalIF":11.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754539/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-Scar Effects of Micropatterned Hydrogel after Glaucoma Drainage Device Implantation.

IF 11 1区综合性期刊

Research Pub Date : 2025-01-22 eCollection Date: 2025-01-01 DOI: 10.34133/research.0561

Yiling Han, Qiangwang Geng, Aimeng Dong, Menglu Jiang, Jingyi Ma, Wulian Song, Pan Fan, Yuanyuan Li, Jiawen Gao, Fenghua Zhang, Jinsong Leng, Huiping Yuan

{"title":"Anti-Scar Effects of Micropatterned Hydrogel after Glaucoma Drainage Device Implantation.","authors":"Yiling Han, Qiangwang Geng, Aimeng Dong, Menglu Jiang, Jingyi Ma, Wulian Song, Pan Fan, Yuanyuan Li, Jiawen Gao, Fenghua Zhang, Jinsong Leng, Huiping Yuan","doi":"10.34133/research.0561","DOIUrl":"10.34133/research.0561","url":null,"abstract":"Excessive fibrosis is the primary factor for the failure of glaucoma drainage device (GDD) implantation. Thus, strategies to suppress scar formation in GDD implantation are crucial. Although it is known that in implanted medical devices, microscale modification of the implant surface can modulate cell behavior and reduce the incidence of fibrosis, in the field of ophthalmic implants, especially the modification and effects of hydrogel micropatterns have rarely been reported. Here, we designed the patterned gelatin/acrylamide double network hydrogel and developed an innovative GDD with micropattern to suppress inflammatory and fibroblast activation after GDD implantation. Pattern topography suppressed F-actin expression and mitigated actin-dependent nuclear migration of myocardin-related transcription factor A (MRTF-A) during the proliferative phase after GDD implantation. Ultimately, the expression of α-smooth muscle actin (α-SMA), a key fibrosis-related gene product, was suppressed. Moreover, the modified GDD effectively controlled intraocular pressure (IOP), mitigated fibrous formation, and remodeled extracellular matrix (ECM) collagen distribution in vivo. Therefore, the novel GDD with surface patterning interventions provides a promising strategy to inhibit scar formation after GDD implantation and raise the efficacy of GDD implantation.","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0561"},"PeriodicalIF":11.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751202/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Altered Atlas of Exercise-Responsive MicroRNAs Revealing miR-29a-3p Attacks Armored and Cold Tumors and Boosts Anti-B7-H3 Therapy.

IF 11 1区综合性期刊

Research Pub Date : 2025-01-22 eCollection Date: 2025-01-01 DOI: 10.34133/research.0590

Jie Mei, Zhiwen Luo, Yun Cai, Renwen Wan, Zhiwen Qian, Jiahui Chu, Yaying Sun, Yuxin Shi, Ying Jiang, Yan Zhang, Yongmei Yin, Shiyi Chen

{"title":"Altered Atlas of Exercise-Responsive MicroRNAs Revealing miR-29a-3p Attacks Armored and Cold Tumors and Boosts Anti-B7-H3 Therapy.","authors":"Jie Mei, Zhiwen Luo, Yun Cai, Renwen Wan, Zhiwen Qian, Jiahui Chu, Yaying Sun, Yuxin Shi, Ying Jiang, Yan Zhang, Yongmei Yin, Shiyi Chen","doi":"10.34133/research.0590","DOIUrl":"10.34133/research.0590","url":null,"abstract":"Increasing evidence has shown that physical exercise remarkably inhibits oncogenesis and progression of numerous cancers and exercise-responsive microRNAs (miRNAs) exert a marked role in exercise-mediated tumor suppression. In this research, expression and prognostic values of exercise-responsive miRNAs were examined in breast cancer (BRCA) and further pan-cancer types. In addition, multiple independent public and in-house cohorts, in vitro assays involving multiple, macrophages, fibroblasts, and tumor cells, and in vivo models were utilized to uncover the tumor-suppressive roles of miR-29a-3p in cancers. Here, we reported that miR-29a-3p was the exercise-responsive miRNA, which was lowly expressed in tumor tissues and associated with unfavorable prognosis in BRCA. Mechanistically, miR-29a-3p targeted macrophages, fibroblasts, and tumor cells to down-regulate B7 homolog 3 (B7-H3) expression. Single-cell RNA sequencing (scRNA-seq) and cytometry by time-of-flight (CyTOF) demonstrated that miR-29a-3p attacked the armored and cold tumors, thereby shaping an immuno-hot tumor microenvironment (TME). Translationally, liposomes were developed and loaded with miR-29a-3p (lipo@miR-29a-3p), and lipo@miR-29a-3p exhibited promising antitumor effects in a mouse model with great biocompatibility. In conclusion, we uncovered that miR-29a-3p is a critical exercise-responsive miRNA, which attacked armored and cold tumors by inhibiting B7-H3 expression. Thus, miR-29a-3p restoration could be an alternative strategy for antitumor therapy.","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0590"},"PeriodicalIF":11.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Networked Intelligent Elderly Care Model Based on Nursing Robots to Achieve Healthy Aging.

IF 11 1区综合性期刊

Research Pub Date : 2025-01-22 eCollection Date: 2025-01-01 DOI: 10.34133/research.0592

Wenting Ji, Ruzhen Luo, Yumei Sun, Maiping Yang, Yueheng Liu, Hua Chen, Dongmei Lin, Ziyi Su, Guangming Tao, Diansheng Chen, Hongyu Sun

引用次数: 0

Terahertz Science and Technology in Astronomy, Telecommunications, and Biophysics.

IF 11 1区综合性期刊

Research Pub Date : 2025-01-22 eCollection Date: 2025-01-01 DOI: 10.34133/research.0586

Jing Li, Xianjin Deng, Yangmei Li, Jie Hu, Wei Miao, Changxing Lin, Jun Jiang, Shengcai Shi

{"title":"Terahertz Science and Technology in Astronomy, Telecommunications, and Biophysics.","authors":"Jing Li, Xianjin Deng, Yangmei Li, Jie Hu, Wei Miao, Changxing Lin, Jun Jiang, Shengcai Shi","doi":"10.34133/research.0586","DOIUrl":"10.34133/research.0586","url":null,"abstract":"This paper reviews recent developments and key advances in terahertz (THz) science, technology, and applications, focusing on 3 core areas: astronomy, telecommunications, and biophysics. In THz astronomy, it highlights major discoveries and ongoing projects, emphasizing the role of advanced superconducting technologies, including superconductor-insulator-superconductor (SIS) mixers, hot electron boundedness spectroscopy (HEB), transition-edge sensors (TESs), and kinetic inductance detectors (KIDs), while exploring prospects in the field. For THz telecommunication, it discusses progress in solid-state sources, new communication technologies operating within the THz band, and diverse modulation methods that enhance transmission capabilities. In THz biophysics, the focus shifts to the physical modulation of THz waves and their impact across biological systems, from whole organisms to cellular and molecular levels, emphasizing nonthermal effects and fundamental mechanisms. This review concludes with an analysis of the challenges and perspectives shaping the future of THz technology.","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0586"},"PeriodicalIF":11.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep Learning-Enabled Integration of Histology and Transcriptomics for Tissue Spatial Profile Analysis. 深度学习支持组织空间剖面分析的组织学和转录组学集成。

IF 11 1区综合性期刊

Research Pub Date : 2025-01-17 eCollection Date: 2025-01-01 DOI: 10.34133/research.0568

Yongxin Ge, Jiake Leng, Ziyang Tang, Kanran Wang, Kaicheng U, Sophia Meixuan Zhang, Sen Han, Yiyan Zhang, Jinxi Xiang, Sen Yang, Xiang Liu, Yi Song, Xiyue Wang, Yuchen Li, Junhan Zhao

{"title":"Deep Learning-Enabled Integration of Histology and Transcriptomics for Tissue Spatial Profile Analysis.","authors":"Yongxin Ge, Jiake Leng, Ziyang Tang, Kanran Wang, Kaicheng U, Sophia Meixuan Zhang, Sen Han, Yiyan Zhang, Jinxi Xiang, Sen Yang, Xiang Liu, Yi Song, Xiyue Wang, Yuchen Li, Junhan Zhao","doi":"10.34133/research.0568","DOIUrl":"https://doi.org/10.34133/research.0568","url":null,"abstract":"Spatially resolved transcriptomics enable comprehensive measurement of gene expression at subcellular resolution while preserving the spatial context of the tissue microenvironment. While deep learning has shown promise in analyzing SCST datasets, most efforts have focused on sequence data and spatial localization, with limited emphasis on leveraging rich histopathological insights from staining images. We introduce GIST, a deep learning-enabled gene expression and histology integration for spatial cellular profiling. GIST employs histopathology foundation models pretrained on millions of histology images to enhance feature extraction and a hybrid graph transformer model to integrate them with transcriptome features. Validated with datasets from human lung, breast, and colorectal cancers, GIST effectively reveals spatial domains and substantially improves the accuracy of segmenting the microenvironment after denoising transcriptomics data. This enhancement enables more accurate gene expression analysis and aids in identifying prognostic marker genes, outperforming state-of-the-art deep learning methods with a total improvement of up to 49.72%. GIST provides a generalizable framework for integrating histology with spatial transcriptome analysis, revealing novel insights into spatial organization and functional dynamics.","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0568"},"PeriodicalIF":11.0,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11739434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Performance Edge-Contact Monolayer Molybdenum Disulfide Transistors. 高性能边缘接触单层二硫化钼晶体管。

IF 11 1区综合性期刊

Research Pub Date : 2025-01-17 eCollection Date: 2025-01-01 DOI: 10.34133/research.0593

Jiankun Xiao, Xiong Xiong, Xinhang Shi, Shiyuan Liu, Shenwu Zhu, Yue Zhang, Ru Huang, Yanqing Wu

{"title":"High-Performance Edge-Contact Monolayer Molybdenum Disulfide Transistors.","authors":"Jiankun Xiao, Xiong Xiong, Xinhang Shi, Shiyuan Liu, Shenwu Zhu, Yue Zhang, Ru Huang, Yanqing Wu","doi":"10.34133/research.0593","DOIUrl":"https://doi.org/10.34133/research.0593","url":null,"abstract":"Edge contact is essential for achieving the ultimate device pitch scaling of stacked nanosheet transistors with monolayer 2-dimensional (2D) channels. However, due to large edge-contact resistance between 2D channels and contact metal, there is currently a lack of high-performance edge-contact device technology for 2D material channels. Here, we report high-performance edge-contact monolayer molybdenum disulfide (MoS2) field-effect transistors (FETs) utilizing well-controlled plasma etching techniques. Plasma etching with pure argon improves the edge dangling bonds and thus improves the edge-contact quality. Edge-contact monolayer MoS2 FET shows good ohmic contact even at cryogenic temperatures (20 K), achieving a record-low contact resistance (R c) of 1.25 kΩ·μm among all edge-contact MoS2 devices. The record-high on-state current of 436 μA/μm and transconductance of 123 μS/μm at V ds = 1 V are achieved on an edge-contact monolayer MoS2 FET with L ch = 120 nm. This work highlights the great potential of edge contacts for high-performance monolayer transition metal dichalcogenide (TMD) material electronics.","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0593"},"PeriodicalIF":11.0,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11739435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Advances in Indocyanine Green-Based Probes for Second Near-Infrared Fluorescence Imaging and Therapy. 吲哚菁绿基二次近红外荧光成像及治疗探针研究进展。

IF 11 1区综合性期刊

Research Pub Date : 2025-01-17 eCollection Date: 2025-01-01 DOI: 10.34133/research.0583

Dehong Hu, Menglei Zha, Hairong Zheng, Duyang Gao, Zonghai Sheng

{"title":"Recent Advances in Indocyanine Green-Based Probes for Second Near-Infrared Fluorescence Imaging and Therapy.","authors":"Dehong Hu, Menglei Zha, Hairong Zheng, Duyang Gao, Zonghai Sheng","doi":"10.34133/research.0583","DOIUrl":"https://doi.org/10.34133/research.0583","url":null,"abstract":"Fluorescence imaging, a highly sensitive molecular imaging modality, is being increasingly integrated into clinical practice. Imaging within the second near-infrared biological window (NIR-II; 1,000 to 1,700 nm), also referred to as shortwave infrared, has received substantial attention because of its markedly reduced autofluorescence, deeper tissue penetration, and enhanced spatiotemporal resolution as compared to traditional near-infrared (NIR) imaging. Indocyanine green (ICG), a US Food and Drug Administration-approved NIR fluorophore, has long been used in clinical applications, including blood vessel angiography, vascular perfusion monitoring, and tumor detection. Recent advancements in NIR-II imaging technology have revitalized interest in ICG, revealing its extended tail fluorescence beyond 1,000 nm and reaffirming its potential as a clinically translatable NIR-II fluorophore for in vivo imaging and theranostic applications for diagnosing various diseases. This review emphasizes the notable advances in the use of ICG and its derivatives for NIR-II imaging and image-guided therapy from both fundamental and clinical perspectives. We also provide a concise conclusion and discuss the challenges and future opportunities with NIR-II imaging using clinically approved fluorophores.","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0583"},"PeriodicalIF":11.0,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11739436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intratonsillar Immunotherapy: A Convenient and Effective Alternative to Subcutaneous Immunotherapy for Allergic Rhinitis. 扁桃腺内免疫治疗：一种代替皮下免疫治疗变应性鼻炎方便有效的方法。

IF 11 1区综合性期刊

Research Pub Date : 2025-01-16 eCollection Date: 2025-01-01 DOI: 10.34133/research.0573

Tian Gu, Wei Zhang, Lu Tan, Rong Xiang, Peiqiang Liu, Jingyu Huang, Qin Deng, Yuqin Deng, Zezhang Tao, Shiming Chen, Yu Xu

{"title":"Intratonsillar Immunotherapy: A Convenient and Effective Alternative to Subcutaneous Immunotherapy for Allergic Rhinitis.","authors":"Tian Gu, Wei Zhang, Lu Tan, Rong Xiang, Peiqiang Liu, Jingyu Huang, Qin Deng, Yuqin Deng, Zezhang Tao, Shiming Chen, Yu Xu","doi":"10.34133/research.0573","DOIUrl":"https://doi.org/10.34133/research.0573","url":null,"abstract":"Allergen-specific immunotherapy (AIT) is the only treatment that addresses the root cause of immunoglobulin E (IgE)-mediated allergies, but conventional methods face challenges with treatment duration, patient compliance, and adverse effects. In this study, we propose intratonsillar immunotherapy (ITIT) as a new effective and safer route for AIT. Prior to clinical trials, we analyzed tonsil samples from human subjects to assess immune responses, measuring interleukin-4 (IL-4), IL-21, total IgE (tIgE), and allergen-specific IgE concentrations using ELISA and BioIC. Our results indicated that tonsils contained higher levels of allergen-specific IgE compared to peripheral blood. In the clinical phase, 120 allergic rhinitis (AR) patients were treated with either 3 intratonsillar allergen injections over 2 months or conventional subcutaneous immunotherapy (SCIT) over 1 year. ITIT demonstrated superior and faster symptom relief, especially in younger patients, while requiring markedly fewer doses and injections than SCIT. Immunological analysis revealed reduced eosinophil counts, increased regulatory T (Treg) and follicular regulatory T (TFR) cell levels, and a favorable shift in cytokine profiles. Adverse events were minimal, and the treatment showed high patient compliance. These findings suggest that ITIT could provide an effective, safer, and more convenient alternative to AIT.","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0573"},"PeriodicalIF":11.0,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735709/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0