Research最新文献

{"title":"Gecko Toe Pad-Inspired Robotic Gripper with Rapidly and Precisely Tunable Adhesion.","authors":"Shuai Li, Hongmiao Tian, Xijing Zhu, Mingxin Liu, Xiangmeng Li, Jinyou Shao","doi":"10.34133/research.0687","DOIUrl":"https://doi.org/10.34133/research.0687","url":null,"abstract":"<p><p>Gecko-inspired dry adhesives have shown great potential in the field of robotics. However, there is still a large gap between current artificial adhesive-based grippers and natural geckos, especially in terms of precise and fast control of adhesion, which is an important capability for robotic gripper systems, since the targets to be gripped may vary in size and weight (including thin, fragile, soft, and deformable), and manipulation must be fast to meet high productivity requirements. Here, we propose a robotic gripper that is able to switch adhesion rapidly (in less than 0.5 s) to grasp and release objects of various sizes and weights (such as glass substrates, fragile silicon wafers, and deformable polyethylene terephthalate films) by mimicking the self-peeling behavior of gecko toe pads. The gripper retains the fast and stable manipulation of the conventional mechanical gripper, which is more reliable and has a higher load capacity than stimulus-responsive switchable adhesives. Systematic experimental and theoretical studies provide insights into the construction and analysis of the self-peeling model and mechanism to identify certain crucial parameters affecting the self-peeling behavior. Furthermore, a strategy for active adhesion control (i.e., precise adhesion modulation) is integrated by introducing a preset peeling angle <i>θ_B</i> , providing the gripper with a quantitative criterion for adjusting the adhesion strength (0 to 82.77 kPa) according to the requirements of practical applications. The gripper has great potential to be an alternative end-operating gripper for robotic systems, opening an avenue for the development of robotic manipulation.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0687"},"PeriodicalIF":11.0,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12015100/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering Cup-Shaped Nanomotors for Promoting Cell Internalization and Synergistic Tumor Therapy.","authors":"Ning Feng, Longteng Liang, Yufang Liu","doi":"10.34133/research.0623","DOIUrl":"https://doi.org/10.34133/research.0623","url":null,"abstract":"<p><p>Self-propulsion enzymatic nanomotors have shown tremendous potential in the field of diagnostics. In a study led by Wang and coworkers, nanoenzyme-driven cup-shaped nanomotors were designed for enhanced cell penetration and synergistic photodynamic/thermal treatments under single near-infrared laser irradiation. By combining the concepts of self-propulsion enzymatic nanomotors and synergistic dual-modal therapy, this work provides a new idea and tool for the application of nanomotors in the biomedical field.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0623"},"PeriodicalIF":11.0,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12012295/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144034192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Freeze-Derived Anisotropic Porous Microparticles for Engineered Mesenchymal Stem Cell Loading and Wound Healing.","authors":"Rongwei Cai, Shuangshuang Miao, Xinyue Cao, Min Nie, Yuanjin Zhao","doi":"10.34133/research.0668","DOIUrl":"https://doi.org/10.34133/research.0668","url":null,"abstract":"<p><p>Hydrogel microparticles that can effectively deliver mesenchymal stem cells (MSCs) are expected to accelerate wound repair progress. Attempts in the area are focusing on improving the functions of the microparticles and MSCs to promote the therapeutic effect. Here, inspired by the topological morphology of ice branches, we propose novel freeze-derived anisotropic porous microparticles for hepatocyte growth factor (HGF)-overexpressing MSCs (MSCs^HGF) loading and wound healing. The microparticles were fabricated by introducing microfluidic methacrylated gelatin pre-gel droplets into low-temperature silicone oil, followed by photo-cross-linking and freeze-drying processes. Drawing an advantage from the biocompatible chemical composition and the structured pore arrangement of the microparticles, MSCs^HGF can be efficiently encapsulated and released, maintaining continuous HGF secretion to enhance cell migration and support vascular regeneration. Leveraging these characteristics, we have shown that MSCs^HGF-loaded porous microparticles could substantially promote angiogenesis, polarize macrophages toward the M2 phenotype, and reduce inflammation during the wound repair process, consequently enhancing skin wound repair efficiency. Thus, we believe that our MSCs^HGF-integrated freeze-derived anisotropic porous microparticles hold promising prospects for clinical wound-healing applications.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0668"},"PeriodicalIF":11.0,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12012297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Perilla frutescens</i> Leaf-Derived Extracellular Vesicle-Like Particles Carry Pab-miR-396a-5p to Alleviate Psoriasis by Modulating IL-17 Signaling.","authors":"Yali Liu, Shanmin Tao, Zhengwei Zhang, Tianjiao Li, Haoran Wang, Jiankang Mu, Yunke Wu, Ziheng He, Cheng Zhang, Dominique Jasmin Lunter, Peng Cao","doi":"10.34133/research.0675","DOIUrl":"https://doi.org/10.34133/research.0675","url":null,"abstract":"<p><p>Psoriasis, a chronic inflammatory skin disorder, remains challenging to treat due to poor skin barrier penetration, limited efficacy, and adverse effects of current therapies. Natural plant-derived extracellular vesicle-like particles (EVPs) have emerged as biocompatible carriers for bioactive molecules. Among various medicinal plants screened, <i>Perilla frutescens</i> leaf-derived EVPs (PLEVPs) exhibited strong anti-inflammatory and antioxidant effects. By incorporating PLEVPs into a hydrogel formulation, we enhanced their stability, retention at psoriatic lesions, and transdermal delivery efficiency. In vivo studies demonstrated that the PLEVPs markedly alleviated psoriasis symptoms in both preventive and therapeutic mouse models, outperforming conventional treatments. This effect was attributed to reduced oxidative stress, modulation of Treg cells, and promotion of keratinocyte apoptosis. Transcriptomic analysis revealed enrichment of the interleukin-17 (IL-17) signaling pathway, a major driver of psoriasis, while small RNA sequencing identified pab-miR396a-5p, an endogenous microRNA (miRNA) within PLEVPs, as a key regulator. Mechanistic studies showed that pab-miR396a-5p targets the 3'-untranslated region of plant heat shock protein 83a, a homolog of mammalian heat shock protein 90, leading to the suppression of nuclear factor-kappa B and Janus kinase/signal transducers and activators of transcription signaling, inhibiting the IL-17 signaling pathway. Validation using lipid nanoparticles encapsulating pab-miR396a-5p mimics confirmed comparable therapeutic effects. This study highlights the potential of plant-derived EVPs as carriers of endogenous miRNAs, enabling interkingdom communication and offering a scalable platform for psoriasis therapy.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0675"},"PeriodicalIF":11.0,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12003952/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Photoelectrocatalytic Water Purification System for Simultaneous Removal of Organic Pollutants and Heavy Metal Ions in Water.","authors":"Peng Zhou, Yingming Xu, Tianhong Cui","doi":"10.34133/research.0654","DOIUrl":"https://doi.org/10.34133/research.0654","url":null,"abstract":"<p><p>A new photoelectrocatalytic water purification system was investigated by combining photocatalysis and electrochemistry. This configuration achieves simultaneous removal of both organic compounds and inorganic heavy metal ions from water by taking a carbon electrode as the working electrode and another electrode coated with a photocatalyst as the counter electrode. A negative bias potential is imparted onto the working electrode to induce the reduction of heavy metal ions, whereas the photocatalytic degradation of organic pollutants on the counter electrode is amplified via the transfer of photoexcited electrons from the counter electrode to the working electrode. Evaluations conducted in bulk solutions demonstrated that photoelectrocatalysis surpassed photocatalysis by yielding an organic matter degradation efficiency 2.3 times higher, successfully degrading 98% of a 10 μM methylene blue solution within 2 h. Simultaneously, the system realized the recovery of heavy metal ions, including copper, lead, and cadmium. This new photoelectrocatalytic water purification system was further integrated with microchannels, and the testing data affirm the substantial potential for system miniaturization.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0654"},"PeriodicalIF":11.0,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12000651/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143981190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gas-Generating Photocatalytic Agents for Bacterial Infection Treatment.","authors":"Yanling Hu, Kaiqi Yang, Ning Li, Dongliang Yang, Heng Dong","doi":"10.34133/research.0672","DOIUrl":"https://doi.org/10.34133/research.0672","url":null,"abstract":"<p><p>Bacterial infections markedly strain healthcare systems financially, compounded by the rise of drug-resistant strains and biofilm-associated infections. Gas therapy has emerged as a notable solution, disrupting biofilms and targeting resistant bacteria through controlled gas release mechanisms. However, achieving precise and controlled gas release remains a critical challenge for the successful implementation of gas therapy. In this perspective, we summarize recent advancements in photocatalytic gas release for treating bacterial infections. It also outlines crucial challenges that must be addressed to fully leverage this promising therapeutic strategy, enhancing its precision and effectiveness in clinical settings.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0672"},"PeriodicalIF":11.0,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12000652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144046229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"B-Lymphoid Tyrosine Kinase Crosslinks Redox and Apoptosis Signaling Networks to Promote the Survival of Transplanted Bone Marrow Mesenchymal Stem Cells.","authors":"Fei Zhang, Tao Wang, Lei Wei, Zhihong Xie, Lijun Wang, Hong Luo, Fanchao Li, Qinglin Kang, Wentao Dong, Jian Zhang, Xuesong Zhu, Chuan Wang, Liang Liang, Wuxun Peng","doi":"10.34133/research.0660","DOIUrl":"https://doi.org/10.34133/research.0660","url":null,"abstract":"<p><p>Stress-induced apoptosis presents an obstacle to bone marrow mesenchymal stem cell (BMSC) transplantation to repair steroid-induced osteonecrosis of the femoral head (SONFH). Thus, appropriate intervention strategies should be explored to mitigate this. In our previous study, we discovered a new subgroup of BMSCs-the oxidative stress-resistant BMSCs (OSR-BMSCs)-which can survive the oxidative stress microenvironment in the osteonecrotic area, through a mechanism that currently remains unclear. In this study, we found that B-lymphoid tyrosine kinase (BLK) may be the crucial factor regulating the oxidative stress resistance of OSR-BMSCs, as it is highly expressed in these cells. Knockdown of BLK eliminated oxidative stress resistance, aggravated oxidative stress-induced apoptosis, reduced the survival of OSR-BMSCs in the oxidative stress microenvironment of the osteonecrotic area, and greatly weakened the transplantation efficacy of OSR-BMSCs for SONFH. By contrast, BLK was weakly expressed in oxidative stress-sensitive BMSCs (OSS-BMSCs). Overexpression of BLK in susceptible OSS-BMSCs allowed them to acquire oxidative stress resistance, inhibited oxidative stress-induced apoptosis, promoted their survival in the osteonecrotic area, and improved the transplantation efficacy of OSS-BMSCs for SONFH. Mechanistically, BLK concurrently activates redox and apoptotic signaling networks through its tyrosine kinase activity, which confers oxidative stress resistance to BMSCs and inhibits their stress-induced apoptosis of BMSCs. Herein, we report that OSR-BMSCs have intrinsic oxidative stress resistance that is conferred and mediated by BLK. This finding provides a potential new intervention strategy for improving the survival of transplanted BMSCs and the therapeutic efficacy of BMSC transplantation for SONFH.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0660"},"PeriodicalIF":11.0,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11999575/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ProstaNet: A Novel Geometric Vector Perceptrons-Graph Neural Network Algorithm for Protein Stability Prediction in Single- and Multiple-Point Mutations with Experimental Validation.","authors":"Tianjian Liang, Ze-Yu Sun, Rieko Ishima, Xiang-Qun Xie, Ying Xue, Wei Li, Zhiwei Feng","doi":"10.34133/research.0674","DOIUrl":"https://doi.org/10.34133/research.0674","url":null,"abstract":"<p><p>Proteins play a critical role in biology and biopharma due to their specificity and minimal side effects. Predicting the effects of mutations on protein stability is vital but experimentally challenging. Deep learning offers an efficient solution to this problem. In the present work, we introduced ProstaNet, a deep learning framework that predicts stability changes resulting from single- and multiple-point mutations using geometric vector perceptrons-graph neural network for 3-dimensional feature processing. For training ProstaNet, we meticulously crafted ProstaDB, a comprehensive and pristine thermodynamics repository, including 3,784 single-point mutations and 1,642 multiple-point mutations. We also created thermodynamic looping for enlarging the limited data size of multiple-point mutation and applied an innovative clustering method to generate a standard testing set of multiple-point mutation. Besides, we identified residue scoring as the most important encoding method in protein properties prediction. With these innovations, ProstaNet accurately predicts thermostability changes for both single-point and multiple-point mutations without showing any bias. ProstaNet achieves an accuracy of 0.75, outperforming existing methods for single-point mutation prediction, including ThermoMPNN (0.63), PoPMuSiC^sym (0.66), MUPRO (0.52), and FoldX (0.71). ProstaNet also achieves a 1.3-fold increase in accuracy compared to FoldX for multiple-point mutation predictions. Validated by experiment, 4 out of 5 single-point mutation predictions (80%) and all multiple-point mutation predictions (100%) for HuJ3 mutants were accurate, demonstrating the potential benefits of ProstaNet for protein engineering and drug development.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0674"},"PeriodicalIF":11.0,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11997553/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144043910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptive Multiobjective Evolutionary Generative Adversarial Network for Metaverse Network Intrusion Detection.","authors":"Dikai Xu, Bin Cao","doi":"10.34133/research.0665","DOIUrl":"https://doi.org/10.34133/research.0665","url":null,"abstract":"<p><p>The convergence of the Metaverse and the Internet of Things (IoT) paves the way for extensive data interaction between connected devices and digital twins; however, this simultaneously introduces considerable cybersecurity threats, including data breaches, ransomware, and device tampering. Existing intrusion detection algorithms struggle to effectively defend against emerging cyberattacks in the rapidly evolving Metaverse environment. Designing effective neural networks for intrusion detection algorithms relies heavily on expert experience, making the manual process time-consuming and often yielding suboptimal results. This paper addresses a critical gap in cybersecurity for Metaverse devices, which are often overlooked in traditional detection methods, and proposes an adaptive multiobjective evolutionary generative adversarial network (AME-GAN) as a novel, scalable solution for optimizing network intrusion detection. An inversely proportional hybrid attention-based long short-term memory GAN is proposed, combining GANs to generate minority class samples and alleviate the imbalance problem in training datasets, which has long hindered accurate intrusion detection. Additionally, an adaptive evolutionary neural architecture search algorithm for the supernet of the GAN is designed to guide the mutation direction of the supernet, enhancing the training stability. This paper further introduces a double mutation multiobjective evolutionary neural architecture search algorithm, integrating both the multiobjective evolutionary algorithm and the neural architecture search to optimize accuracy, real-time performance, and model diversity-a crucial aspect for Metaverse devices with diverse hardware constraints. Experiments conducted on 3 well-known datasets-NSL-KDD, UNSW-NB15, and CIC-IDS2017-demonstrate that AME-GAN outperforms state-of-the-art approaches, with improvements of 0.32% in accuracy, 0.31% in F1 score, 0.47% in precision, and 0.37% in recall. This paper offers a promising, adaptive framework to enhance cybersecurity in the Metaverse, improving detection performance and real-time applicability, and contributing to the future of network intrusion detection in next-generation digital environments.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0665"},"PeriodicalIF":11.0,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11997310/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144049887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early Growth Response Gene 1 Benefits Autoimmune Disease by Promoting Regulatory T Cell Differentiation as a Regulator of Foxp3.","authors":"Liu Yang, Xinyan Han, Mengxue Wang, Xiaojuan Zhang, Lupeng Wang, Nuo Xu, Hui Wu, Hailian Shi, Weidong Pan, Fei Huang, Xiaojun Wu","doi":"10.34133/research.0662","DOIUrl":"https://doi.org/10.34133/research.0662","url":null,"abstract":"<p><p>Foxp3⁺ regulatory T (T_reg) cells, as one of the subtypes of CD4⁺ T cells, are the crucial gatekeeper in the pathogenesis of self-antigen reactive diseases. In this context, we demonstrated that the selective ablation of early growth response gene 1 (Egr-1) in CD4⁺ T cells exacerbated experimental autoimmune encephalomyelitis (EAE) in murine models. The absence of Egr-1 in CD4⁺ T cells, obtained from EAE mice and naïve CD4⁺ T cells, impeded the differentiation and influence of T_reg. Importantly, in CD4⁺ T cells of multiple sclerosis patients, both Egr-1 and Foxp3 were found to decrease. Further studies showed that distinct from the classical Smad3 route, TGF-β could activate Egr-1 through the Raf-Erk signaling route to promote Foxp3 genetic modulation, thereby promoting T_reg cell differentiation and reducing EAE inflammation. A novel natural Egr-1 agonist, calycosin, was found to attenuate EAE progression by regulating the differentiation of T_reg. Together, the above results indicate the value of Egr-1, as a novel Foxp3 transactivator, for the differentiation of T_reg cells in the development of self-antigen reactive diseases.</p>","PeriodicalId":21120,"journal":{"name":"Research","volume":"8 ","pages":"0662"},"PeriodicalIF":11.0,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11997311/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}