Research最新文献_第5页

Ultrasensitive Terahertz Label-Free Metasensors Enabled by Quasi-Bound States in the Continuum. 超灵敏太赫兹无标签元传感器由连续体中的准束缚态实现

IF 11 1区综合性期刊

Research Pub Date : 2024-09-26 eCollection Date: 2024-01-01 DOI: 10.34133/research.0483

Ride Wang, Lingyu Song, Hao Ruan, Quanlong Yang, Xiao Yang, Xiaobao Zhang, Rundong Jiang, Xiangmin Shi, Alexander P Shkurinov

{"title":"Ultrasensitive Terahertz Label-Free Metasensors Enabled by Quasi-Bound States in the Continuum.","authors":"Ride Wang, Lingyu Song, Hao Ruan, Quanlong Yang, Xiao Yang, Xiaobao Zhang, Rundong Jiang, Xiangmin Shi, Alexander P Shkurinov","doi":"10.34133/research.0483","DOIUrl":"https://doi.org/10.34133/research.0483","url":null,"abstract":"Advanced sensing devices based on metasurfaces have emerged as a revolutionary platform for innovative label-free biosensors, holding promise for early diagnostics and the detection of low-concentration analytes. Here, we developed a chip-based ultrasensitive terahertz (THz) metasensor, leveraging a quasi-bound state in the continuum (q-BIC) to address the challenges associated with intricate operations in trace biochemical detection. The metasensor design features an open-ring resonator metasurface, which supports magnetic dipole q-BIC combining functionalized gold nanoparticles (AuNPs) bound with a specific antibody. The substantial enhancement in THz-analyte interactions, facilitated by the potent near-field enhancement enabled by the q-BICs, results in a substantial boost in biosensor sensitivity by up to 560 GHz/refractive index units. This methodology allows for the detection of conjugated antibody-AuNPs for cardiac troponin I at concentrations as low as 0.5 pg/ml. These discoveries deliver valuable insight for AuNP-based trace biomolecule sensing and pave the path for the development of chip-scale biosensors with profound light-matter interactions.","PeriodicalId":21120,"journal":{"name":"Research","volume":"7 ","pages":"0483"},"PeriodicalIF":11.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11425342/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary "Beigeing" Fat Contains More Phosphatidylserine and Enhances Mitochondrial Function while Counteracting Obesity. 膳食中的 "米色 "脂肪含有更多磷脂酰丝氨酸，可增强线粒体功能，同时抵消肥胖。

IF 11 1区综合性期刊

Research Pub Date : 2024-09-26 eCollection Date: 2024-01-01 DOI: 10.34133/research.0492

Yanbing Zhou, Defeng Ling, Liyi Wang, Ziye Xu, Wenjing You, Wentao Chen, Qiuyun Nong, Teresa G Valencak, Tizhong Shan

{"title":"Dietary \"Beigeing\" Fat Contains More Phosphatidylserine and Enhances Mitochondrial Function while Counteracting Obesity.","authors":"Yanbing Zhou, Defeng Ling, Liyi Wang, Ziye Xu, Wenjing You, Wentao Chen, Qiuyun Nong, Teresa G Valencak, Tizhong Shan","doi":"10.34133/research.0492","DOIUrl":"https://doi.org/10.34133/research.0492","url":null,"abstract":"Activation of mitochondrial function and heat production in adipose tissue by the modification of dietary fat is a promising strategy against obesity. However, as an important source of lipids for ketogenic and daily diets, the function of fats extracted from different adipose tissue sites was largely unknown. In this study, we illustrated the function of fats extracted from adipose tissues with different \"beigeing\" properties in the ketogenic diet and identified lipid profiles of fats that facilitate energy expenditure. We found that the anti-obesity effect of ketogenic diets was potentiated by using \"beigeing\" fat [porcine subcutaneous adipose tissue (SAT)] as a major energy-providing ingredient. Through lipidomic analyses, phosphatidylserine (PS) was identified as a functional lipid activating thermogenesis in adipose tissue. Moreover, in vivo studies showed that PS induces adipose tissue thermogenesis and alleviates diet-induced obesity in mice. In vitro studies showed that PS promotes UCP1 expression and lipolysis of adipocytes. Mechanistically, PS promoted mitochondrial function in adipocytes via the ADCY3-cAMP-PKA-PGC1α pathway. In addition, PS-PGC1a binding may affect the stability of the PGC1α protein, which further augments PS-induced thermogenesis. These results demonstrated the efficacy of dietary SAT fats in diminishing lipid accumulation and the underlying molecular mechanism of PS in enhancing UCP1 expression and mitochondrial function. Thus, our findings suggest that as dietary fat, \"beigeing\" fat provides more beneficial lipids that contribute to the improvement of mitochondrial function, including PS, which may become a novel, nonpharmacological therapy to increase energy expenditure and counteract obesity and its related diseases.","PeriodicalId":21120,"journal":{"name":"Research","volume":"7 ","pages":"0492"},"PeriodicalIF":11.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11425158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine Learning Enables Comprehensive Prediction of the Relative Protein Abundance of Multiple Proteins on the Protein Corona. 机器学习可全面预测蛋白质日冕上多种蛋白质的相对蛋白丰度

IF 11 1区综合性期刊

Research Pub Date : 2024-09-25 eCollection Date: 2024-01-01 DOI: 10.34133/research.0487

Xiuhao Fu, Chao Yang, Yunyun Su, Chunling Liu, Haoye Qiu, Yanyan Yu, Gaoxing Su, Qingchen Zhang, Leyi Wei, Feifei Cui, Quan Zou, Zilong Zhang

{"title":"Machine Learning Enables Comprehensive Prediction of the Relative Protein Abundance of Multiple Proteins on the Protein Corona.","authors":"Xiuhao Fu, Chao Yang, Yunyun Su, Chunling Liu, Haoye Qiu, Yanyan Yu, Gaoxing Su, Qingchen Zhang, Leyi Wei, Feifei Cui, Quan Zou, Zilong Zhang","doi":"10.34133/research.0487","DOIUrl":"https://doi.org/10.34133/research.0487","url":null,"abstract":"Understanding protein corona composition is essential for evaluating their potential applications in biomedicine. Relative protein abundance (RPA), accounting for the total proteins in the corona, is an important parameter for describing the protein corona. For the first time, we comprehensively predicted the RPA of multiple proteins on the protein corona. First, we used multiple machine learning algorithms to predict whether a protein adsorbs to a nanoparticle, which is dichotomous prediction. Then, we selected the top 3 performing machine learning algorithms in dichotomous prediction to predict the specific value of RPA, which is regression prediction. Meanwhile, we analyzed the advantages and disadvantages of different machine learning algorithms for RPA prediction through interpretable analysis. Finally, we mined important features about the RPA prediction, which provided effective suggestions for the preliminary design of protein corona. The service for the prediction of RPA is available at http://www.bioai-lab.com/PC_ML.","PeriodicalId":21120,"journal":{"name":"Research","volume":"7 ","pages":"0487"},"PeriodicalIF":11.0,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11423712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142336565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of PCSK9: A Promising Enhancer for Anti-PD-1/PD-L1 Immunotherapy. 抑制 PCSK9：抗-PD-1/PD-L1 免疫疗法的希望增强剂。

IF 11 1区综合性期刊

Research Pub Date : 2024-09-25 eCollection Date: 2024-01-01 DOI: 10.34133/research.0488

Shengbo Sun, Jingxin Ma, Tingting Zuo, Jinyao Shi, Liting Sun, Cong Meng, Wenlong Shu, Zhengyang Yang, Hongwei Yao, Zhongtao Zhang

{"title":"Inhibition of PCSK9: A Promising Enhancer for Anti-PD-1/PD-L1 Immunotherapy.","authors":"Shengbo Sun, Jingxin Ma, Tingting Zuo, Jinyao Shi, Liting Sun, Cong Meng, Wenlong Shu, Zhengyang Yang, Hongwei Yao, Zhongtao Zhang","doi":"10.34133/research.0488","DOIUrl":"https://doi.org/10.34133/research.0488","url":null,"abstract":"Immune checkpoint therapy, such as programmed cell death protein 1/programmed death-ligand 1 (PD-1/PD-L1) blockade, has achieved remarkable results in treating various tumors. However, most cancer patients show a low response rate to PD-1/PD-L1 blockade, especially those with microsatellite stable/mismatch repair-proficient colorectal cancer subtypes, which indicates an urgent need for new approaches to augment the efficacy of PD-1/PD-L1 blockade. Cholesterol metabolism, which involves generating multifunctional metabolites and essential membrane components, is also instrumental in tumor development. In recent years, inhibiting proprotein convertase subtilisin/kexin type 9 (PCSK9), a serine proteinase that regulates cholesterol metabolism, has been demonstrated to be a method enhancing the antitumor effect of PD-1/PD-L1 blockade to some extent. Mechanistically, PCSK9 inhibition can maintain the recycling of major histocompatibility protein class I, promote low-density lipoprotein receptor-mediated T-cell receptor recycling and signaling, and modulate the tumor microenvironment (TME) by affecting the infiltration and exclusion of immune cells. These mechanisms increase the quantity and enhance the antineoplastic effect of cytotoxic T lymphocyte, the main functional immune cells involved in anti-PD-1/PD-L1 immunotherapy, in the TME. Therefore, combining PCSK9 inhibition therapy with anti-PD-1/PD-L1 immunotherapy may provide a novel option for improving antitumor effects and may constitute a promising research direction. This review concentrates on the relationship between PCSK9 and cholesterol metabolism, systematically discusses how PCSK9 inhibition potentiates PD-1/PD-L1 blockade for cancer treatment, and highlights the research directions in this field.","PeriodicalId":21120,"journal":{"name":"Research","volume":"7 ","pages":"0488"},"PeriodicalIF":11.0,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11423609/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Summer-Wet Hydrologic Cycle during the Middle Miocene of the United States: New Evidence from Fossil Fungi. 美国中新世的夏季-湿润水文循环：来自真菌化石的新证据。

IF 11 1区综合性期刊

Research Pub Date : 2024-09-24 eCollection Date: 2024-01-01 DOI: 10.34133/research.0481

Jennifer M K O'Keefe, Matthew J Pound, Ingrid C Romero, Noelia B Nuñez Otaño, Martha E Gibson, Jessica McCoy, Margaret E Alden, C Jolene Fairchild, Julia Fitzpatrick, Emily Hodgson, Taylor Horsfall, Savannah Jones, June E Lennex-Stone, Christopher A Marsh, Alyssa A Patel, Tyler M Spears, Laikin Tarlton, Liberty F Smallwood, O L VanderEspt, Jeremyah R Cabrera, Cortland F Eble, William C Rember, James E Starnes, Mac H Alford, Alyson Brink, Sophie Warny

{"title":"Summer-Wet Hydrologic Cycle during the Middle Miocene of the United States: New Evidence from Fossil Fungi.","authors":"Jennifer M K O'Keefe, Matthew J Pound, Ingrid C Romero, Noelia B Nuñez Otaño, Martha E Gibson, Jessica McCoy, Margaret E Alden, C Jolene Fairchild, Julia Fitzpatrick, Emily Hodgson, Taylor Horsfall, Savannah Jones, June E Lennex-Stone, Christopher A Marsh, Alyssa A Patel, Tyler M Spears, Laikin Tarlton, Liberty F Smallwood, O L VanderEspt, Jeremyah R Cabrera, Cortland F Eble, William C Rember, James E Starnes, Mac H Alford, Alyson Brink, Sophie Warny","doi":"10.34133/research.0481","DOIUrl":"https://doi.org/10.34133/research.0481","url":null,"abstract":"Hydrologic reconstructions from North America are largely unknown for the Middle Miocene. Examination of fungal palynomorph assemblages coupled with traditional plant-based palynology permits delineation of local, as opposed to regional, climate signals and provides a baseline for study of ancient fungas. Here, the Fungi in a Warmer World project presents paleoecology and paleoclimatology of 351 fungal morphotypes from 3 sites in the United States: the Clarkia Konservat-Lagerstätte site (Idaho), the Alum Bluff site (Florida), and the Bouie River site (Mississippi). Of these, 83 fungi are identified as extant taxa and 41 are newly reported from the Miocene. Combining new plant-based paleoclimatic reconstructions with funga-based paleoclimate reconstructions, we demonstrate cooling and hydrologic changes from the Miocene climate optimum to the Serravallian. In the southeastern United States, this is comparable to that reconstructed with pollen and paleobotany alone. In the northwestern United States, cooling is greater than indicated by other reconstructions and hydrology shifts seasonally, from no dry season to a dry summer season. Our results demonstrate the utility of fossil fungi as paleoecologic and paleoclimatic proxies and that warmer than modern geological time intervals do not match the \"wet gets wetter, dry gets drier\" paradigm. Instead, both plants and fungi show an invigorated hydrological cycle across mid-latitude North America.","PeriodicalId":21120,"journal":{"name":"Research","volume":"7 ","pages":"0481"},"PeriodicalIF":11.0,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11420851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blind Brush Biopsy: Quantification of Epstein-Barr Virus and Its Host DNA Methylation in the Detection of Nasopharyngeal Carcinoma. 盲刷活检：定量检测鼻咽癌中的 Epstein-Barr 病毒及其宿主 DNA 甲基化。

IF 11 1区综合性期刊

Research Pub Date : 2024-09-24 eCollection Date: 2024-01-01 DOI: 10.34133/research.0475

Caoli Tang, Xizhao Li, Yumeng Zhang, Ting Zhou, Xiaojing Yang, Ying Liao, Tongmin Wang, Yongqiao He, Wenqiong Xue, Weihua Jia, Xiaohui Zheng

{"title":"Blind Brush Biopsy: Quantification of Epstein-Barr Virus and Its Host DNA Methylation in the Detection of Nasopharyngeal Carcinoma.","authors":"Caoli Tang, Xizhao Li, Yumeng Zhang, Ting Zhou, Xiaojing Yang, Ying Liao, Tongmin Wang, Yongqiao He, Wenqiong Xue, Weihua Jia, Xiaohui Zheng","doi":"10.34133/research.0475","DOIUrl":"https://doi.org/10.34133/research.0475","url":null,"abstract":"Background: The nasopharyngeal brush sampling can effectively collect samples from the nasopharynx. The blind brush sampling does not require the guidance of endoscopy, which is favorable for implementation and dissemination in the community. This study explored methylation markers for nasopharyngeal carcinoma (NPC) at both Epstein-Barr virus (EBV) and its host genome levels, aiming to construct a blind brushing diagnostic method. Methods: EBV DNA capture and methylation sequencing and GEO Illumina 450K methylation array data were used respectively for the discovery of EBV and host methylation markers. The diagnostic method was built in training cohort (n = 347) and validated in an independent validation cohort (n = 155). Results: A total of 1 EBV methylation marker (BILF2) and 6 host methylation markers (ITGA4, IMPA2, ITPKB, PI9, AMIGO2, and VAV3) were identified. Both EBV and host methylation markers were almost exclusively detected in NPC samples, with negligible detection in control samples. In validation cohort, the diagnostic method that included only the EBV BILF2 marker showed a sensitivity and specificity of 80.22% and 98.44%, respectively. When combining the EBV-derived marker BILF2 with the host-derived marker IMPA2, the diagnostic method's sensitivity increased to 84.62%, while the specificity remained unchanged (IDI = 4.4%, P = 0.0419). Conclusion: Overall, the blind nasopharyngeal brushing diagnostic method, combining EBV and host methylation markers, showed great potential in NPC detection and could promote its application in nonclinical screening of NPC.","PeriodicalId":21120,"journal":{"name":"Research","volume":"7 ","pages":"0475"},"PeriodicalIF":11.0,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11420652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioinspired Wet Adhesive Proanthocyanidins Microneedles for Ocular Wound Healing. 用于眼部伤口愈合的生物启发湿式粘合剂原花青素微针。

IF 11 1区综合性期刊

Research Pub Date : 2024-09-24 eCollection Date: 2024-01-01 DOI: 10.34133/research.0485

Bin Kong, Rui Liu, Tiantian Kong, Yuanjin Zhao

{"title":"Bioinspired Wet Adhesive Proanthocyanidins Microneedles for Ocular Wound Healing.","authors":"Bin Kong, Rui Liu, Tiantian Kong, Yuanjin Zhao","doi":"10.34133/research.0485","DOIUrl":"https://doi.org/10.34133/research.0485","url":null,"abstract":"Microneedles have shown considerable potential in treating ocular diseases, yet enhancing their architecture and functionality to improve therapeutic efficacy poses marked challenges. Here, inspired by the antioxidant strategy of blueberries and the wet adhesive mechanism of clingfish, we construct hierarchical and multifunctional microneedles. These microneedles possess both wet adhesive and antioxidant properties, making them highly effective for ocular wound healing. Constructed using polyacrylic acid-N-hydroxysuccinimide-based hydrogel with hexagonal structures, these generated microneedles ensure strong adhesion in wet environments. Furthermore, by incorporating proanthocyanidins (pAc) into the tips, the microneedle is imparted with excellent competence to scavenge reactive oxygen species (ROS). In the rat model of ocular alkali burns, the designed microneedle not only exhibited robust adhesion and desirable antioxidant properties in the moist ocular environment but also facilitated sustained drug release and effective treatment. These results suggest that our bioinspired microneedles with multifunctional properties offer substantial advancement over conventional approaches, positioning them as promising candidates for versatile wound healing applications.","PeriodicalId":21120,"journal":{"name":"Research","volume":"7 ","pages":"0485"},"PeriodicalIF":11.0,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11420907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142336564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melting and Rapid Solidification of Lunar Regolith Particles Returned by Chang'E-5 Mission. 嫦娥五号任务返回的月球碎屑的熔化和快速凝固。

IF 11 1区综合性期刊

Research Pub Date : 2024-09-23 eCollection Date: 2024-01-01 DOI: 10.34133/research.0486

Xian Zhang, Yiwei Liu, Shaofan Zhao, Jian Song, Wei Yao, Weihua Wang, Zhigang Zou, Mengfei Yang

{"title":"Melting and Rapid Solidification of Lunar Regolith Particles Returned by Chang'E-5 Mission.","authors":"Xian Zhang, Yiwei Liu, Shaofan Zhao, Jian Song, Wei Yao, Weihua Wang, Zhigang Zou, Mengfei Yang","doi":"10.34133/research.0486","DOIUrl":"10.34133/research.0486","url":null,"abstract":"Melting and solidification of lunar regolith are pivotal for comprehending the evolutionary dynamics of lunar volcanism, geology, and impact history. Additionally, insights gained from these processes can contribute to the advancement of in situ resource utilization technologies, for instance additive manufacturing and resource extraction systems. Herein, we conduct the direct observation of the melting and rapid solidification of lunar particles returned by the Chang'E 5 mission. The melting temperature and melting sequence were obtained. Bubble generation, growth, and release were clearly observed, with a maximum bubble diameter of 5 µm, which is supposed to be according to the release of volatiles that embedded in the particles. During the solidification process, evident crystallization occurred with incremental crystal growth rate approximately of 27 nm/s. Scanning electron microscopy and energy-dispersive x-ray spectroscopy results verified that the Fe-rich mineral crystalizes first. These results would improve the understanding of the evolution of lunar volcanism, geology, and impact history.","PeriodicalId":21120,"journal":{"name":"Research","volume":"7 ","pages":"0486"},"PeriodicalIF":11.0,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11417502/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142308533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amino Turbo Chirality and Its Asymmetric Control. 氨基酸涡轮手性及其不对称控制

IF 11 1区综合性期刊

Research Pub Date : 2024-09-19 eCollection Date: 2024-01-01 DOI: 10.34133/research.0474

Ting Xu, Yu Wang, Shengzhou Jin, Anis U Rahman, Xianghua Yan, Qingkai Yuan, Hao Liu, Jia-Yin Wang, Wenxin Yan, Yinchun Jiao, Ruibin Liang, Guigen Li

{"title":"Amino Turbo Chirality and Its Asymmetric Control.","authors":"Ting Xu, Yu Wang, Shengzhou Jin, Anis U Rahman, Xianghua Yan, Qingkai Yuan, Hao Liu, Jia-Yin Wang, Wenxin Yan, Yinchun Jiao, Ruibin Liang, Guigen Li","doi":"10.34133/research.0474","DOIUrl":"10.34133/research.0474","url":null,"abstract":"A series of new targets containing 3 chiral elements of central, orientational, and turbo chirality have been designed and synthesized asymmetrically. The absolute configurations and conformations of these types of chirality were concurrently controlled by using chiral sulfonimine auxiliary and unambiguously determined by x-ray diffraction analysis. These targets include alpha unnatural amino acid derivatives, which may play an important role for drug design, discovery, and development. Three propellers of turbo framework are covalently connected to a chiral C(sp3) center via C(sp2)-C(sp3) bonding along with a C-N axis, while one of them is orientated away from the same carbon chiral center. The turbo or propeller chirality is characterized by 2 types of molecular arrangements of propellers, clockwise (PPP) and counterclockwise (MMM), respectively. The turbo stereogenicity was found to depend on the center chirality of sulfonimine auxiliary instead of the chiral C(sp3) center, i.e., (S)- and (R)-sulfinyl centers led to the asymmetric formation of PPP- and MMM-configurations, respectively. Computational studies were conducted on relative energies for rotational barriers of a turbo target along the C-N anchor and the transition pathway between 2 enantiomers meeting our experimental observations. This work is anticipated to have a broad impact on chemical, biomedical, and materials sciences in the future.","PeriodicalId":21120,"journal":{"name":"Research","volume":"7 ","pages":"0474"},"PeriodicalIF":11.0,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11411161/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut Microbiota-Metabolite-Brain Axis Reconstitution Reverses Sevoflurane-Induced Social and Synaptic Deficits in Neonatal Mice. 肠道微生物群-代谢产物-脑轴重建可逆转七氟醚诱导的新生小鼠社交和突触缺陷

IF 11 1区综合性期刊

Research Pub Date : 2024-09-19 eCollection Date: 2024-01-01 DOI: 10.34133/research.0482

Youyi Zhao, Sanxing Ma, Lirong Liang, Shuhui Cao, Ze Fan, Danyi He, Xiaotong Shi, Yao Zhang, Bing Liu, Meiting Zhai, Shengxi Wu, Fang Kuang, Hui Zhang

{"title":"Gut Microbiota-Metabolite-Brain Axis Reconstitution Reverses Sevoflurane-Induced Social and Synaptic Deficits in Neonatal Mice.","authors":"Youyi Zhao, Sanxing Ma, Lirong Liang, Shuhui Cao, Ze Fan, Danyi He, Xiaotong Shi, Yao Zhang, Bing Liu, Meiting Zhai, Shengxi Wu, Fang Kuang, Hui Zhang","doi":"10.34133/research.0482","DOIUrl":"https://doi.org/10.34133/research.0482","url":null,"abstract":"Background: The mechanisms underlying social dysfunction caused by repeated sevoflurane in early life remain unclear. Whether the gut microbiota-metabolite-brain axis is involved in the mechanism of sevoflurane developmental neurotoxicity still lacks report. Methods: Mice received 3% sevoflurane at postnatal day (PND) 6, 7, and 8 for 2 h per day. Metagenomic sequencing and untargeted metabolomic analysis were applied to investigate the effects of sevoflurane on gut microbiota and metabolism. The animal social behavior and the synaptic development were analyzed during PND 35. Subsequently, fecal microbiota transplantation (FMT) from the control group and bile acid administration were performed to see the expected rescuing effect on socially related behaviors that were impaired by repeated sevoflurane exposure in the mice. Results: In the 3-chamber test, sevoflurane-exposed mice spent less time with stranger mice compared with the control group. The density of both the apical and basal spine decreased in mice exposed to sevoflurane. In addition, repeated sevoflurane exposure led to a notable alteration in the gut microbiota and metabolite synthesis, particularly bile acid. FMT reduced the production of intestinal bile acid and attenuated the effect of sevoflurane exposure on social function and synaptic development. Cholestyramine treatment mimics the protective effects of FMT. Conclusions: The gut microbiota-metabolite-brain axis underlies social dysfunction caused by sevoflurane exposure in early age, and bile acid regulation may be a promising intervention to this impairment.","PeriodicalId":21120,"journal":{"name":"Research","volume":"7 ","pages":"0482"},"PeriodicalIF":11.0,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11411162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0