Research in microbiology最新文献_第4页

d-aspartate, an amino-acid important for human health, supports anaerobic respiration in several Campylobacter species D- 天门冬氨酸是一种对人体健康非常重要的氨基酸，它支持弯曲杆菌中多种细菌的厌氧呼吸。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-09-01 DOI: 10.1016/j.resmic.2024.104219

{"title":"d-aspartate, an amino-acid important for human health, supports anaerobic respiration in several Campylobacter species","authors":"","doi":"10.1016/j.resmic.2024.104219","DOIUrl":"10.1016/j.resmic.2024.104219","url":null,"abstract":"<div>Despite being classified as microaerophilic microorganisms, most Campylobacter species can grow anaerobically, using formate or molecular hydrogen (H2) as electron donors, and various nitrogenous and sulfurous compounds as electron acceptors. Herein, we showed that both l-asparagine (l-Asn) and l-aspartic acid (l-Asp) bolster H2-driven anaerobic growth in several Campylobacter species, whereas the d-enantiomer form of both asparagine (d-Asn) and aspartic acid (d-Asp) only increased anaerobic growth in Campylobacter concisus strain 13826 and Campylobacter ureolyticus strain NCTC10941. A gene annotated as racD encoding for a putative d/l-Asp racemase was identified in the genome of both strains. Disruption of racD in Cc13826 resulted in the inability of the mutant strain to use either d-enantiomer during anaerobic growth. Hence, our results suggest that the racD gene is required for campylobacters to use either d-Asp or d-Asn. The use of d-Asp by various human opportunistic bacterial pathogens, including C. concisus, C. ureolyticus, and also possibly select strains of Campylobacter gracilis, Campylobacter rectus and Campylobacter showae, is significant, because d-Asp is an important signal molecule for both human nervous and neuroendocrine systems. To our knowledge, this is the first report of pathogens scavenging a d-amino acid essential for human health.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 7","pages":"Article 104219"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141470480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofilms in soils: The evidence about sessile versus planktonic microorganisms needs revisiting 土壤中的生物膜：需要重新审视有关无柄微生物与浮游微生物的证据。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-09-01 DOI: 10.1016/j.resmic.2024.104204

引用次数: 0

Effects of ultraviolet and photosynthetically active radiation on morphogenesis, antioxidants and photoprotective defense mechanism in a hot-spring cyanobacterium Nostoc sp. strain VKB02 紫外线和光合有效辐射对温泉蓝藻 Nostoc sp. 菌株 VKB02 的形态发生、抗氧化剂和光保护防御机制的影响

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-07-01 DOI: 10.1016/j.resmic.2024.104180

Nasreen Amin , Rajeshwar P. Sinha , Vinod K. Kannaujiya

{"title":"Effects of ultraviolet and photosynthetically active radiation on morphogenesis, antioxidants and photoprotective defense mechanism in a hot-spring cyanobacterium Nostoc sp. strain VKB02","authors":"Nasreen Amin , Rajeshwar P. Sinha , Vinod K. Kannaujiya","doi":"10.1016/j.resmic.2024.104180","DOIUrl":"10.1016/j.resmic.2024.104180","url":null,"abstract":"<div>The continuous increase in global temperature and ultraviolet radiation (UVR) causes profound impacts on the growth and physiology of photosynthetic microorganisms. The hot-spring cyanobacteria have a wide range of mitigation mechanisms to cope up against current unsustainable environmental conditions. In the present investigation, we have explored the indispensable mitigation strategies of an isolated hot-spring cyanobacterium Nostoc sp. strain VKB02 under simulated ultraviolet (UV-A, UV-B) and photosynthetically active radiation (PAR). The adaptive morphological changes were more significantly observed under PAB (PAR, UV-A, and UV-B) exposure as compared to P and PA (PAR and UV-A) irradiations. PAB exposure also exhibited a marked decline in pigment composition and photosynthetic efficiency by multi-fold increment of free radicals. To counteract the oxidative stress, enzymatic and non-enzymatic antioxidants defense were significantly enhanced many folds under PAB exposure as compared to the control. In addition, the cyanobacterium has also produced shinorine as a strong free radicals scavenger and excellent UV absorber for effective photoprotection against UV radiation. Therefore, the hot-spring cyanobacterium Nostoc sp. strain VKB02 has unique defense strategies for survival under prolonged lethal UVR conditions. This study will help in the understanding of environment-induced defense strategies and production of highly value-added green photo-protectants for commercial applications.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 5","pages":"Article 104180"},"PeriodicalIF":2.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139417962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The murein endopeptidase MepA regulated by MtrAB and MprAB participate in cell wall homeostasis 受 MtrAB 和 MprAB 调控的粘蛋白内肽酶 MepA 参与细胞壁的平衡。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-07-01 DOI: 10.1016/j.resmic.2024.104188

Feng Peng , Yu Zou , Xiuxia Liu , Yankun Yang , Jing Chen , Jianqi Nie , Danni Huang , Zhonghu Bai

引用次数: 0

Multiple SigB homologues govern the transcription of the ssgBp promoter in the sporulation–specific ssgB gene in Streptomyces coelicolor A3(2) 多个 SigB 同源物控制着 A3(2)链霉菌孢子特异性 ssgB 基因中 ssgBp 启动子的转录。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-07-01 DOI: 10.1016/j.resmic.2024.104201

Rachel Javorova , Beatrica Sevcikova , Bronislava Rezuchova, Renata Novakova, Filip Opaterny, Dominika Csolleiova, Lubomira Feckova, Jan Kormanec

{"title":"Multiple SigB homologues govern the transcription of the ssgBp promoter in the sporulation–specific ssgB gene in Streptomyces coelicolor A3(2)","authors":"Rachel Javorova , Beatrica Sevcikova , Bronislava Rezuchova, Renata Novakova, Filip Opaterny, Dominika Csolleiova, Lubomira Feckova, Jan Kormanec","doi":"10.1016/j.resmic.2024.104201","DOIUrl":"10.1016/j.resmic.2024.104201","url":null,"abstract":"<div>Unlike Bacillus subtilis, Streptomyces coelicolor contains nine SigB homologues of the stress-response sigma factor SigB. By using a two-plasmid system, we previously identified promoters recognized by these sigma factors. Almost all promoters were recognized by several SigB homologues. However, no specific sequences of these promoters were found. One of these promoters, ssgBp, was selected to examine this cross-recognition in the native host. It controls the expression of the sporulation-specific gene ssgB. Using a luciferase reporter, the activity of this promoter in S. coelicolor and nine mutant strains lacking individual sigB homologous genes showed that sgBp is dependent on three sigma factors, SigH, SigN, and SigI. To determine which nucleotides in the-10 region are responsible for the selection of a specific SigB homologue, promoters mutated at the last three nucleotide positions were tested in the two-plasmid system. Some mutant promoters were specifically recognized by a distinct set of SigB homologues. Analysis of these mutant promoters in the native host showed the role of these nucleotides. A conserved nucleotide A at position 5 was essential for promoter activity, and two variable nucleotides at positions 4 and 6 were responsible for the partial selectivity of promoter recognition by SigB homologues.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 5","pages":"Article 104201"},"PeriodicalIF":2.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The influence of the phylum Planctomycetota in the environmental resistome Planctomycetota 门在环境抗性组中的影响。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-07-01 DOI: 10.1016/j.resmic.2024.104196

Ofélia Godinho , Damien P. Devos , Sandra Quinteira , Olga M. Lage

引用次数: 0

Whole genome analysis, thermal and UV-tolerance of Lactococcus phage BIM BV-114 isolated from cheese brine 从奶酪盐水中分离出的乳球菌噬菌体 BIM BV-114 的全基因组分析、耐热性和耐紫外线性。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-07-01 DOI: 10.1016/j.resmic.2024.104203

Aliaksandra Herasimovich, Artur Akhremchuk, Leonid Valentovich, Anastasiya Sidarenka

{"title":"Whole genome analysis, thermal and UV-tolerance of Lactococcus phage BIM BV-114 isolated from cheese brine","authors":"Aliaksandra Herasimovich, Artur Akhremchuk, Leonid Valentovich, Anastasiya Sidarenka","doi":"10.1016/j.resmic.2024.104203","DOIUrl":"10.1016/j.resmic.2024.104203","url":null,"abstract":"<div>Lactococcus phages that belong to the genus Ceduovirus are among the three most frequently isolated phage groups infecting Lactococcus lactis starter strains in dairy plants. In this study, we characterized virulent Lactococcus phage BIM BV-114 isolated from industrial cheese brine in Belarus and identified as Ceduovirus. The bacteriophage demonstrated a relatively short lytic cycle (latent period of 23 ± 5 min, lysis time of 90 ± 5 min), high thermal stability (inactivation after 7 min at 95 °C in skimmed milk) and tolerance to UV radiation (inactivation time – 15 min), indicating adaptation for better persistence in dairy facilities. The genome of the phage BIM BV-114 (21 499 bp; 37 putative open reading frames) has a similar organization to that of other Ceduovirus phages. RLf1_00140 and RLf_00050 gene products, found in the early genes region, may be involved in the sensitivity of phage to the lactococcal abortive infection mechanisms AbiV and AbiQ, respectively. Furthermore, nucleotide deletion, observed in the middle region of the gene encoding putative tape measure protein (RLf1_00300), is possibly responsible for increased thermal tolerance of phage BIM BV-114. Together, these findings will contribute to a better knowledge of virulent Lactococcus phages and the development of effective methods of their control for dairy technologies.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 5","pages":"Article 104203"},"PeriodicalIF":2.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140857949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical characterization and mutational analysis of the NurA protein from the hyperthermophilic euryarchaeon Thermococcus barophilus Ch5 超嗜热真菌嗜热球菌 Ch5 的 NurA 蛋白的生化特征和突变分析。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-07-01 DOI: 10.1016/j.resmic.2024.104189

Guangyu Ma , Tan Lin , Peng Cao , Philippe Oger , Kunming Dong , Li Miao , Likui Zhang

{"title":"Biochemical characterization and mutational analysis of the NurA protein from the hyperthermophilic euryarchaeon Thermococcus barophilus Ch5","authors":"Guangyu Ma , Tan Lin , Peng Cao , Philippe Oger , Kunming Dong , Li Miao , Likui Zhang","doi":"10.1016/j.resmic.2024.104189","DOIUrl":"10.1016/j.resmic.2024.104189","url":null,"abstract":"<div>Archaeal NurA protein plays a key role in producing 3′-single stranded DNA used for homologous recombination repair, together with HerA, Mre11, and Rad50. Herein, we describe biochemical characteristics and roles of key amino acid residues of the NurA protein from the hyperthermophilic euryarchaeon Thermococcus barophilus Ch5 (Tba-NurA). Tba-NurA possesses 5′–3′ exonuclease activity for degrading DNA, displaying maximum efficiency at 45 °C–65 °C and at pH 8.0 in the presence of Mn2+. The thermostable Tba-NurA also possesses endonuclease activity capable of nicking plasmid DNA and circular ssDNA. Mutational data demonstrate that residue D49 of Tba-NurA is essential for exonuclease activity and is involved in binding ssDNA since the D49A mutant lacked exonuclease activity and reduced ssDNA binding. The R96A and R129A mutants had no detectable dsDNA binding, suggesting that residues R96 and R129 are important for binding dsDNA. The abolished degradation activity and reduced dsDNA binding of the D120A mutant suggest that residue D120 is essential for degradation activity and dsDNA binding. Additionally, residues Y392 and H400 are important for exonuclease activity since these mutations resulted in exonuclease activity loss. To our knowledge, it is the first report on biochemical characterization and mutational analysis of the NurA protein from Thermococcus.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 5","pages":"Article 104189"},"PeriodicalIF":2.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139973333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of a PRD1-like phage uncovers the carriage of three putative conjugative plasmids in clinical Burkholderia contaminans 一种类似 PRD1 的噬菌体的分离发现了临床伯克霍尔德菌污染菌中携带的三种假定共轭质粒

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-07-01 DOI: 10.1016/j.resmic.2024.104202

Cassandra R. Stanton , Steve Petrovski , Steven Batinovic

{"title":"Isolation of a PRD1-like phage uncovers the carriage of three putative conjugative plasmids in clinical Burkholderia contaminans","authors":"Cassandra R. Stanton , Steve Petrovski , Steven Batinovic","doi":"10.1016/j.resmic.2024.104202","DOIUrl":"10.1016/j.resmic.2024.104202","url":null,"abstract":"<div>The Burkholderia cepacia complex (Bcc) is a group of increasingly multi-drug resistant opportunistic bacteria. This resistance is driven through a combination of intrinsic factors and the carriage of a broad range of conjugative plasmids harbouring virulence determinants. Therefore, novel treatments are required to treat and prevent further spread of these virulence determinants. In the search for phages infective for clinical Bcc isolates, CSP1 phage, a PRD1-like phage was isolated. CSP1 phage was found to require pilus machinery commonly encoded on conjugative plasmids to facilitate infection of Gram-negative bacteria genera including Escherichia and Pseudomonas. Whole genome sequencing and characterisation of one of the clinical Burkholderia isolates revealed it to be Burkholderia contaminans. B. contaminans 5080 was found to contain a genome of over 8 Mbp encoding multiple intrinsic resistance factors, such as efflux pump systems, but more interestingly, carried three novel plasmids encoding multiple putative virulence factors for increased host fitness, including antimicrobial resistance. Even though PRD1-like phages are broad host range, their use in novel antimicrobial treatments shouldn't be dismissed, as the dissemination potential of conjugative plasmids is extensive. Continued survey of clinical bacterial strains is also key to understanding the spread of antimicrobial resistance determinants and plasmid evolution.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 5","pages":"Article 104202"},"PeriodicalIF":2.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0923250824000330/pdfft?md5=db2b5303bfc7ffe5081639e2734bdbd5&pid=1-s2.0-S0923250824000330-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140613764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Termitidicoccus mucosus gen. nov. sp. nov. a novel Verrucomicrobiota species isolated from Reticulitermes chinensis gives insights of high adaptability of symbiotic bacteria to termite gut ecosystem 从网纹蚁中分离出的新的白蚁微生物群（Termitidicoccus mucosus gen.

IF 2.6 4区生物学

Research in microbiology Pub Date : 2024-05-01 DOI: 10.1016/j.resmic.2023.104173

Cheng Mei , Yu Shi , Yu Wang , Zhengyong Qiu , Hong Yang

{"title":"Termitidicoccus mucosus gen. nov. sp. nov. a novel Verrucomicrobiota species isolated from Reticulitermes chinensis gives insights of high adaptability of symbiotic bacteria to termite gut ecosystem","authors":"Cheng Mei , Yu Shi , Yu Wang , Zhengyong Qiu , Hong Yang","doi":"10.1016/j.resmic.2023.104173","DOIUrl":"10.1016/j.resmic.2023.104173","url":null,"abstract":"<div>Verrucomicrobiota is widely distributed in various habitats including insect guts. It was found to be prevalent in almost all investigated termite guts, whereas their physiological functions are not very clear. In this study we characterized the physiological and genomic properties of Verrucomicrobiota strain TSB47T isolated from Reticulitermes chinensis. The cells of strain TSB47T were Gram-stain-negative, non-motile, and non-spore-forming coccoid with one or more warts. 16S rRNA gene analysis showed that the closest relatives of strain TSB47T were Opitutaceae strain TAV1 and Ereboglobus luteus Ho45T (98.3% and 95.4% sequence similarity, respectively). Whole genome analysis revealed that there are a large number of glycoside hydrolase genes, amino acid metabolism genes, complete Mo-Fe nitrogenase and Fe-Fe nitrogenase gene clusters, as well as cbb3-type cytochrome oxidase gene in the genome of strain TSB47T. Strain TSB47T grows well under anaerobic and microaerophilic conditions with a strong tolerance to oxygen. Physiological and genomic characters of strain TSB47T indicated its high adaptability to termite gut ecosystem. Based on phenotypic and phylogenetic evidence, we suggest strain TSB47T as the type species of a novel genus in the family Opitutaceae, for which the name Termitidicoccus mucosus sp. nov. is proposed. The type strain is TSB47T (CCTCC AB2022447T; KCTC 102044T).</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 4","pages":"Article 104173"},"PeriodicalIF":2.6,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139061872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0