The murein endopeptidase MepA regulated by MtrAB and MprAB participate in cell wall homeostasis

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Research in microbiology Pub Date : 2024-07-01 DOI:10.1016/j.resmic.2024.104188

Feng Peng , Yu Zou , Xiuxia Liu , Yankun Yang , Jing Chen , Jianqi Nie , Danni Huang , Zhonghu Bai

引用次数: 0

Abstract

The complete genome of Corynebacterium glutamicum contain a gene encoding murein endopeptidase MepA which maintain cell wall homeostasis by regulating peptidoglycan biosynthesis. In this study, we investigate the physiological function, localization and regulator of MepA. The result shows that mepA overexpression lead to peptidoglycan degradation and the defects in cell division. MepA-EGFP was shown to localizes exclusively at the cell cell septum. In addition, mepA overexpression increased cell permeability and reduced the resistance of cells to isoniazid, an antibiotic used to treat Mycobacterium tuberculosis infection. Furthermore, transcription analysis showed that mepA affected cell division and membrane transport pathways, and was coordinately regulated by the two-component systems MtrAB and MprAB(CgtS/R2).

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受 MtrAB 和 MprAB 调控的粘蛋白内肽酶 MepA 参与细胞壁的平衡。

谷氨酸棒状杆菌的完整基因组中含有一个编码金黄色葡萄蛋白内肽酶 MepA 的基因，该基因通过调节肽聚糖的生物合成来维持细胞壁的平衡。本研究对 MepA 的生理功能、定位和调节因子进行了研究。结果表明，过表达 MepA 会导致肽聚糖降解和细胞分裂缺陷。结果表明，MepA-mCherry只定位在细胞隔。此外，mepA 的过表达增加了细胞的通透性，降低了细胞对异烟肼（一种用于治疗结核分枝杆菌感染的抗生素）的抗性。此外，转录分析表明，mepA影响细胞分裂和膜运输途径，并受双组分系统MtrAB和MprAB（CgtS/R2）的协调调控。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research in microbiology 生物-微生物学

CiteScore

4.10

自引率

3.80%

发文量

审稿时长

16 days

期刊介绍： Research in Microbiology is the direct descendant of the original Pasteur periodical entitled Annales de l''Institut Pasteur, created in 1887 by Emile Duclaux under the patronage of Louis Pasteur. The Editorial Committee included Chamberland, Grancher, Nocard, Roux and Straus, and the first issue began with Louis Pasteur''s "Lettre sur la Rage" which clearly defines the spirit of the journal:"You have informed me, my dear Duclaux, that you intend to start a monthly collection of articles entitled "Annales de l''Institut Pasteur". You will be rendering a service that will be appreciated by the ever increasing number of young scientists who are attracted to microbiological studies. In your Annales, our laboratory research will of course occupy a central position, but the work from outside groups that you intend to publish will be a source of competitive stimulation for all of us."That first volume included 53 articles as well as critical reviews and book reviews. From that time on, the Annales appeared regularly every month, without interruption, even during the two world wars. Although the journal has undergone many changes over the past 100 years (in the title, the format, the language) reflecting the evolution in scientific publishing, it has consistently maintained the Pasteur tradition by publishing original reports on all aspects of microbiology.