Research in microbiology最新文献_第10页

Prevalence, antimicrobial resistance, and genetic lineages of nasal Staphylococcus aureus among medical students at a Spanish University: Detection of the MSSA-CC398-IEC-type-C subclade. 西班牙一所大学医学生鼻腔金黄色葡萄球菌的流行率、抗菌药耐药性和基因系：发现 MSSA-CC398-IEC-C 型亚支系。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-12-01 DOI: 10.1016/j.resmic.2023.104176

Nerea C. Rosales-González, Margarita González-Martín, Idris Nasir Abdullahi, Ma Teresa Tejedor-Junco, Javier Latorre-Fernández, Carmen Torres

引用次数: 0

Title page 标题页

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/S0923-2508(23)00136-5

引用次数: 0

Phosphate starvation is accompanied by disturbance of intracellular cysteine homeostasis in Escherichia coli 在大肠杆菌中，磷酸盐饥饿伴随着细胞内半胱氨酸稳态的紊乱。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104108

Galina V. Smirnova, Aleksey V. Tyulenev, Kseniya V. Bezmaternykh, Nadezda G. Muzyka, Vadim Y. Ushakov, Oleg N. Oktyabrsky

{"title":"Phosphate starvation is accompanied by disturbance of intracellular cysteine homeostasis in Escherichia coli","authors":"Galina V. Smirnova, Aleksey V. Tyulenev, Kseniya V. Bezmaternykh, Nadezda G. Muzyka, Vadim Y. Ushakov, Oleg N. Oktyabrsky","doi":"10.1016/j.resmic.2023.104108","DOIUrl":"10.1016/j.resmic.2023.104108","url":null,"abstract":"<div>Metabolic rearrangements that occur during depletion of essential nutrients can lead to accumulation of potentially dangerous metabolites. Here we showed that depletion of phosphate (Pi), accompanied by a sharp inhibition of growth and respiration, caused a transient excess of intracellular cysteine due to a decrease in the rate of protein synthesis. High cysteine level can be dangerous due to its ability to produce ROS and reduce Fe3+ to Fenton-reactive Fe2+. To prevent these negative effects, excess cysteine was mainly incorporated into glutathione (GSH), the intracellular level of which increased by 3 times, and was also exported to the medium and partially degraded to form H2S with participation of 3-mercaptopyruvate sulfotransferase (3MST). The addition of Pi to starving cells led to a sharp recovery of respiration and growth, GSH efflux into the medium and K+ influx into the cells. A pronounced coupling of Pi, GSH, and K+ fluxes was shown upon Pi depletion and addition, which may be necessary to maintain the ionic balance in the cytoplasm. We suggest that processes aimed at restoring cysteine homeostasis may be an integral part of the universal response to stress under different types of stress and for different types of bacteria.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 8","pages":"Article 104108"},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10247813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Programmed cell death in Xanthomonas axonopodis pv. glycines is associated with modulation of gene expression resulting in altered states of motility, biofilm and virulence 轴足黄单胞菌的程序性细胞死亡。甘氨酸与基因表达的调节有关，导致运动性、生物膜和毒力状态的改变。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104137

Nilantana C. Bandyopadhyay , Satyendra Gautam

{"title":"Programmed cell death in Xanthomonas axonopodis pv. glycines is associated with modulation of gene expression resulting in altered states of motility, biofilm and virulence","authors":"Nilantana C. Bandyopadhyay , Satyendra Gautam","doi":"10.1016/j.resmic.2023.104137","DOIUrl":"10.1016/j.resmic.2023.104137","url":null,"abstract":"<div>One of the foremost report of apoptosis-like programmed cell death (PCD) came from Xanthomonas axonopodis pv. glycines (Xag), which displayed rapid post-exponential cell death in PCD inducing media (PIM) but not in a non-inducing media (PNIM). The current study aims to decipher for the first time, the advantages of the existence of PCD in this phytopathogenic microorganism. Analysis of RNA-seq under inducing and non-inducing conditions, revealed differential expression of a number of genes related to key physiology of Xag, such as, motility, xanthan biosynthesis and export as well as virulence. A PCD negative mutant Xag M42 displayed diminished virulence and a contrasting transcriptome pattern. In vitro experiments revealed that under PCD inducing condition, Xag produced negligible xanthan gum as well as extracellular amylase, displayed enhanced swarming motility, released copious e-DNA and formed scanty biofilm. Lack of ‘diffusible signalling factor’ production was eliminated as possible reason for PCD-induction. Altogether, it appears that, in planta existence of the pathogen metabolically resembles PNIM, and on being transferred to PIM, the cells experience oxidative stress and circumvents it by adopting PCD as an altruistic response. Survival of the remaining population is encouraged by upregulating motility, detachment from the fragile biofilm to achieve dispersal.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 8","pages":"Article 104137"},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10269190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

trans-translation system is important for maintaining genome integrity during DNA damage in bacteria 反翻译系统在细菌DNA损伤过程中对维持基因组完整性非常重要。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104136

Nagarajan T , Sutharsan Govindarajan , M. Hussain Munavar

{"title":"trans-translation system is important for maintaining genome integrity during DNA damage in bacteria","authors":"Nagarajan T , Sutharsan Govindarajan , M. Hussain Munavar","doi":"10.1016/j.resmic.2023.104136","DOIUrl":"10.1016/j.resmic.2023.104136","url":null,"abstract":"<div>DNA integrity in bacteria is regulated by various factors that act on the DNA. trans-translation has previously been shown to be important for the survival of Escherichia coli cells exposed to certain DNA-damaging agents. However, the mechanisms underlying this sensitivity are poorly understood. In this study, we explored the involvement of the trans-translation system in the maintenance of genome integrity using various DNA-damaging agents and mutant backgrounds. Relative viability assays showed that SsrA-defective cells were sensitive to DNA-damaging agents, such as nalidixic acid (NA), ultraviolet radiation (UV), and methyl methanesulfonate (MMS). The viability of SsrA-defective cells was rescued by deleting sulA, although the expression of SulA was not more pronounced in SsrA-defective cells than in wild-type cells. Live cell imaging using a Gam-GFP fluorescent reporter showed increased double-strand breaks (DSBs) in SsrA-defective cells during DNA damage. We also showed that the ribosome rescue function of SsrA was sufficient for DNA damage tolerance. DNA damage sensitivity can be alleviated by partial uncoupling of transcription and translation by using sub-lethal concentrations of ribosome inhibiting antibiotic (tetracycline) or by mutating the gene coding for RNase H (rnhA). Taken together, our results highlight the importance of trans-translation system in maintaining genome integrity and bacterial survival during DNA damage.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 8","pages":"Article 104136"},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10201239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Skin microbiome bacteria enriched following long sun exposure can reduce oxidative damage 长时间暴露在阳光下后富含的皮肤微生物组细菌可以减少氧化损伤。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104138

Nurit Harel , Navit Ogen-Shtern , Leah Reshef , Dvora Biran , Eliora Z. Ron , Uri Gophna

{"title":"Skin microbiome bacteria enriched following long sun exposure can reduce oxidative damage","authors":"Nurit Harel , Navit Ogen-Shtern , Leah Reshef , Dvora Biran , Eliora Z. Ron , Uri Gophna","doi":"10.1016/j.resmic.2023.104138","DOIUrl":"10.1016/j.resmic.2023.104138","url":null,"abstract":"<div>Sun exposure is harmful to the skin and increases the risk of skin aging and skin cancer. Here we examined the effects of daily exposure to sun radiation on the skin microbiome in order to determine whether skim microbiome bacteria can contribute to protection from solar damage. Skin swabs were collected from ten lifeguards before and after the summer to analyse the skin microbiome. The results indicate that specific skin microbiome bacteria were enriched following the seasonal sun exposure. Especially interesting were two bacterial families - Sphingomonas and Erythrobacteraceae – which may have the ability to protect against UV radiation as they produce potentially protective compounds. We concentrated on a Sphingomonas strain and could show that it was highly resistant to UV irradiation and was able to reduce reactive oxygen species levels in human keratinocytes. These results provide a proof-of-concept for the role of the skin microbiome in protection from solar radiation.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 8","pages":"Article 104138"},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10309411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The importance of the PapR7 C-terminus and amide protons in mediating quorum sensing in Bacillus cereus PapR7 C末端和酰胺质子在介导Bacilluscereus群体感应中的重要性。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104139

Michael Gorgan , Shahar Vanunu Ofri , Emilee R. Engler , Avishag Yehuda , Elizabeth Hutnick , Zvi Hayouka , Michael A. Bertucci

{"title":"The importance of the PapR7 C-terminus and amide protons in mediating quorum sensing in Bacillus cereus","authors":"Michael Gorgan , Shahar Vanunu Ofri , Emilee R. Engler , Avishag Yehuda , Elizabeth Hutnick , Zvi Hayouka , Michael A. Bertucci","doi":"10.1016/j.resmic.2023.104139","DOIUrl":"10.1016/j.resmic.2023.104139","url":null,"abstract":"<div>The opportunistic human pathogen Bacillus cereus controls the expression of key infection-promoting phenotypes using bacterial quorum sensing (QS). QS signal transduction within the species is controlled by an autoinducing peptide, PapR7, and its cognate receptor, PlcR, indicating that the PlcR:PapR interface is a prime target for QS inhibitor development. The C-terminal region of the peptide (PapR7; ADLPFEF) has been successfully employed as a scaffold to develop potent QS modulators. Despite the noted importance of the C-terminal carboxylate and amide protons in crystallographic data, their role in QS activity has yet to be explored. In this study, an N-methyl scan of PapR7 was conducted in conjunction with a C-terminal modification of previously identified B. cereus QS inhibitors. The results indicate that the amide proton at Glu6 and the C-terminal carboxylate are important for effective QS inhibition of the PlcR regulon. Through β-galactosidase and hemolysis assays, a series of QS inhibitors were discovered, including several capable of inhibiting QS with nanomolar potency. These inhibitors, along with the structure–activity data reported, will serve as valuable tools for disrupting the B. cereus QS pathway towards developing novel anti-infective strategies.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 8","pages":"Article 104139"},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41132503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heat shock response in Sulfolobus acidocaldarius and first implications for cross-stress adaptation 嗜酸硫酸藻的热休克反应和交叉应激适应的初步意义。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104106

Arghya Bhowmick , Koustav Bhakta , Mousam Roy , Sayandeep Gupta , Jagriti Das , Shirsha Samanta , Somi Patranabis , Abhrajyoti Ghosh

{"title":"Heat shock response in Sulfolobus acidocaldarius and first implications for cross-stress adaptation","authors":"Arghya Bhowmick , Koustav Bhakta , Mousam Roy , Sayandeep Gupta , Jagriti Das , Shirsha Samanta , Somi Patranabis , Abhrajyoti Ghosh","doi":"10.1016/j.resmic.2023.104106","DOIUrl":"10.1016/j.resmic.2023.104106","url":null,"abstract":"<div>Sulfolobus acidocaldarius, a thermoacidophilic crenarchaeon, frequently encounters temperature fluctuations, oxidative stress, and nutrient limitations in its environment. Here, we employed a high-throughput transcriptomic analysis to examine how the gene expression of S. acidocaldarius changes when exposed to high temperatures (92 °C). The data obtained was subsequently validated using quantitative reverse transcription-PCR (qRT-PCR) analysis. Our particular focus was on genes that are involved in the heat shock response, type-II Toxin-Antitoxin systems, and putative transcription factors. To investigate how S. acidocaldarius adapts to multiple stressors, we assessed the expression of these selected genes under oxidative and nutrient stresses using qRT-PCR analysis. The results demonstrated that the gene thβ encoding the β subunit of the thermosome, as well as hsp14 and hsp20, play crucial roles in the majority of stress conditions. Furthermore, we observed overexpression of at least eight different TA pairs belonging to the type II TA systems under all stress conditions. Additionally, four common transcription factors: FadR, TFEβ, CRISPR loci binding protein, and HTH family protein were consistently overexpressed across all stress conditions, indicating their significant role in managing stress. Overall, this work provides the first insight into molecular players involved in the cross-stress adaptation of S. acidocaldarius.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 8","pages":"Article 104106"},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10247814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Leptospiral cell wall hydrolase (LIC_10271) binding peptidoglycan, lipopolysaccharide, and laminin and the protein show LysM and M23 domains are co-existing in pathogenic species 钩端螺旋体细胞壁水解酶（LIC_10271）结合肽聚糖、脂多糖和层粘连蛋白，该蛋白显示LysM和M23结构域在致病物种中共存。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104107

Abhijit Sarma , Gunasekaran Dhandapani , Homen Phukan , Prasun Kumar Bhunia , Arun Kumar De , Debasis Bhattacharya , T. Jebasingh , Madathiparambil G. Madanan

{"title":"Leptospiral cell wall hydrolase (LIC_10271) binding peptidoglycan, lipopolysaccharide, and laminin and the protein show LysM and M23 domains are co-existing in pathogenic species","authors":"Abhijit Sarma , Gunasekaran Dhandapani , Homen Phukan , Prasun Kumar Bhunia , Arun Kumar De , Debasis Bhattacharya , T. Jebasingh , Madathiparambil G. Madanan","doi":"10.1016/j.resmic.2023.104107","DOIUrl":"10.1016/j.resmic.2023.104107","url":null,"abstract":"<div>Leptospirosis, a global reemerging zoonosis caused by the spirochete Leptospira, has severe human and veterinary implications. Cell wall hydrolase (LIC_10271) with LytM (peptidase M23) and LysM domains are found to be associated with various pathogenic bacteria. These domains regulate effects on extracellular matrix and biofilm components, which promote cell wall remodeling and pathogen dissemination in the host. In this study, we present the cloning, expression, purification, and characterization of LIC_10271. To determine the localization of LIC_10271 within the inner membrane of Leptospira, Triton X-114 subcellular fractionation and immunoblot studies were performed. Furthermore, r-LIC_10271 binds with peptidoglycan, lipopolysaccharide, and laminin in a dose-dependent manner. Analysis of the signal peptide, M23, and LysM domains revealed conservation primarily within the P1 group of Leptospira, which encompasses the most pathogenic species. Moreover, the presence of native-LIC_10271 in the inner membrane and the distribution of M23 and LysM domains across pathogenic strains indicates their potential involvement in the interaction between the host and Leptospira.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 8","pages":"Article 104107"},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9951818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deficient Pseudomonas aeruginosa in MlaA/VacJ outer membrane lipoprotein shows decrease in rhamnolipids secretion, motility, and biofilm formation, and increase in fluoroquinolones susceptibility and innate immune response MlaA/VacJ外膜脂蛋白中的铜绿假单胞菌缺陷显示鼠李糖脂分泌、运动性和生物膜形成减少，氟喹诺酮类药物敏感性和先天免疫反应增加。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104132

M. Kaur , J.M. Buyck , F. Goormaghtigh , J.-L. Decout , N. Mozaheb , M.-P. Mingeot-Leclercq

{"title":"Deficient Pseudomonas aeruginosa in MlaA/VacJ outer membrane lipoprotein shows decrease in rhamnolipids secretion, motility, and biofilm formation, and increase in fluoroquinolones susceptibility and innate immune response","authors":"M. Kaur , J.M. Buyck , F. Goormaghtigh , J.-L. Decout , N. Mozaheb , M.-P. Mingeot-Leclercq","doi":"10.1016/j.resmic.2023.104132","DOIUrl":"10.1016/j.resmic.2023.104132","url":null,"abstract":"<div>Pseudomonas aeruginosa, a Gram-negative bacterium that causes severe hospital acquired infections poses threat by its ability for adaptation to various growth modes and environmental conditions and by its intrinsic resistance to antibiotics. The latter is mainly due to the outer membrane (OM) asymmetry which is maintained by the Mla pathway resulting in the retrograde transport of glycerophospholipids from the OM to the inner membrane. It comprises six Mla proteins, including MlaA, an OM lipoprotein involved in the removal of glycerophospholipids mislocalized at the outer leaflet of OM.To investigate the role of P. aeruginosa OM asymmetry especially MlaA, this study investigated the effect of mlaA deletion on (i) the susceptibility to antibiotics, (ii) the secretion of virulence factors, the motility, biofilm formation, and (iii) the inflammatory response.mlaA deletion in P. aeruginosa ATCC27853 results in phenotypic changes including, an increase in fluoroquinolones susceptibility and in PQS (Pseudomonas Quinolone Signal) and TNF-α release and a decrease in rhamnolipids secretion, motility and biofilm formation.Investigating how the mlaA knockout impacts on antibiotic susceptibility, bacterial virulence and innate immune response will help to elucidate the biological significance of the Mla system and contribute to the understanding of MlaA in P. aeruginosa OM asymmetry.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 8","pages":"Article 104132"},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0923250823001079/pdfft?md5=0ddbed0060c793d80f891fde818c0b30&pid=1-s2.0-S0923250823001079-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10146831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0