Research in microbiology最新文献

Cutibacterium acnes and its complex host interaction in prosthetic joint infection: Current insights and future directions.

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-12-17 DOI: 10.1016/j.resmic.2024.104265

Léa Thoraval, Jennifer Varin-Simon, Xavier Ohl, Frédéric Velard, Fany Reffuveille, Min Tang-Fichaux

引用次数: 0

Novel insights into Bacillus thuringiensis: Beyond its role as a bioinsecticide. 苏云金芽孢杆菌的新见解：超越其作为生物杀虫剂的作用。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-12-13 DOI: 10.1016/j.resmic.2024.104264

Gholamreza Salehi Jouzani, Reza Sharafi, Leandris Argentel-Martínez, Ofelda Peñuelas-Rubio, Ceyda Ozkan, Bengisu Incegul, Rana Goksu, Zehra Hayta, Deniz Yilki, Beyza Yazici, Vildan Hancer, Estibaliz Sansinenea, Jae-Ho Shin, A El-Shabasy, Ugur Azizoglu

引用次数: 0

Characterization and identification of Pseudomonas sp. AW4, an arsenic-resistant and plant growth-promoting bacteria isolated from the soybean (Glycine max L.) rhizosphere.

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-12-06 DOI: 10.1016/j.resmic.2024.104263

Ana L Wevar Oller, Gonzalo Torres Tejerizo, Paola P Pereira, Romina Del Pilar Pramparo, Elizabeth Agostini

{"title":"Characterization and identification of Pseudomonas sp. AW4, an arsenic-resistant and plant growth-promoting bacteria isolated from the soybean (Glycine max L.) rhizosphere.","authors":"Ana L Wevar Oller, Gonzalo Torres Tejerizo, Paola P Pereira, Romina Del Pilar Pramparo, Elizabeth Agostini","doi":"10.1016/j.resmic.2024.104263","DOIUrl":"10.1016/j.resmic.2024.104263","url":null,"abstract":"<p><p>Pseudomonas sp. AW4 is a highly arsenic (As) resistant bacterium with plant growth promoting properties, originally isolated from the soybean (Glycine max L.) rhizosphere. In order to safely use this isolate in diverse bioformulations, its characterization needs to be completed and a reliable identification must be provided. In the present work, we analyzed the morpho-physiological, biochemical and genomic characteristics of Pseudomonas sp. AW4. Identification of the isolate varied according to the parameters analyzed, mainly biochemical and physiological tests or individual genes and phylogenetic analyses. In this regard, we performed massive sequencing of its genome, in order to consistently complete its characterization and identification. Pseudomonas sp. AW4 formed a monophyletic clade with P. urmiensis SWRI10, presenting 3.08 % of unique genes against this reference isolate. More than 70 % of AW4 genes were also shared with P. oryziphila strain 1257 NZ and with P. reidholzensis strain CCOS 865. The search for genes related to As resistance evidenced the presence of the operon arsHRBC. Taken together, results of the present work allow identification of this bacterium as Pseudomonas urmiensis AW4 and open up a number of opportunities to study this strain and understand the mechanisms of arsenic resistance and plant growth promotion.</p>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":" ","pages":"104263"},"PeriodicalIF":2.5,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142795021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The roots of the Institut Pasteur's "Grand Cours".

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-12-05 DOI: 10.1016/j.resmic.2024.104259

Antoine Dampierre, Sandra Legout, Emmanuel Drouin

{"title":"The roots of the Institut Pasteur's \"Grand Cours\".","authors":"Antoine Dampierre, Sandra Legout, Emmanuel Drouin","doi":"10.1016/j.resmic.2024.104259","DOIUrl":"https://doi.org/10.1016/j.resmic.2024.104259","url":null,"abstract":"<p><p>The article presents an analysis of the history of the microbiology course delivered during the inaugural operational year of the Institut Pasteur in Paris. The year 1889 is examined through the lens of three hitherto unknown volumes that bring together the microbiology lectures delivered at the end of the 19th century. The course was entirely independent from the teaching provided by the universities or faculties of medicine and rapidly gained international recognition. Indeed, the course provided the students with the theoretical knowledge of Pasteurian theories regarding the completely new discipline of microbiology and the specific techniques used to cultivate, conserve, and observe microbes. The steady increase in the number of lectures between 1889 and 1914 reflects the expansion of microbiological knowledge during this period. The contributions of researchers such as Émile Roux (1853-1933), Élie Metchnikoff (1845-1916), and Amédée Borrel (1867-1936) illuminated the collaboration and the growing diversification of expertise at the heart of the Institut Pasteur (IP). Furthermore, this study highlights the international influence of the course, as evidenced by the participation of foreign students. It examines the history of the course as a powerful tool for disseminating knowledge of new microbiological techniques and the results of research carried out in Pasteur's laboratories. It also examines how the course served as a political instrument, asserting the authority of the Institut Pasteur in the field of microbiology in France and extending its influence worldwide.</p>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":" ","pages":"104259"},"PeriodicalIF":2.5,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Navigating dual-species fungal biofilms: The competitive and cooperative dynamics of Candidaalbicans.

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-11-28 DOI: 10.1016/j.resmic.2024.104262

Ouassila Bekkal Brikci-Benhabib

引用次数: 0

Characterization of tailocins of Pragia fontium 24613 and the tailocin loci within the family Budviciaceae. Pragia fontium 24613 的尾丝素特征及芽苣苔科（Budviciaceae）内的尾丝素基因座。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-11-23 DOI: 10.1016/j.resmic.2024.104261

Kateřina Snopková, Eva Chaloupková, Matěj Hrala, David Šmajs

{"title":"Characterization of tailocins of Pragia fontium 24613 and the tailocin loci within the family Budviciaceae.","authors":"Kateřina Snopková, Eva Chaloupková, Matěj Hrala, David Šmajs","doi":"10.1016/j.resmic.2024.104261","DOIUrl":"10.1016/j.resmic.2024.104261","url":null,"abstract":"<p><p>Tailocins are nano-scale phage tail-like protein complexes that can mediate antagonistic interactions between closely related bacterial species. While the capacity to produce R-type tailocin was found widely across Gammaproteobacteria, the production of F-type tailocins seems comparatively rare. In this study, we examined the freshwater isolate, Pragia fontium 24613, which can produce both R- and F-type tailocins. We investigated their inhibition spectrum, focusing on clinically relevant enterobacteria, and identified the associated tailocin gene cluster. Transmission electron microscopy confirmed that inactivation of the tape measure protein within the tailocin cluster disrupted R-tailocin production. Comparative analysis of Budviciaceae gene clusters showed high conservation of R-type tailocin genes, whereas F-type tailocin genes were found in only a few species, with little conservation. Our findings indicate a high prevalence of bacteriocin production among underexplored Enterobacteriales species. Detected tailocins showed potential as antimicrobials targeting clinically significant pathogens.</p>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":" ","pages":"104261"},"PeriodicalIF":2.5,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of 5'-UTR nucleotide composition on translation efficiency in Escherichiacoli. 5'-UTR 核苷酸组成对大肠杆菌翻译效率的影响

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-11-15 DOI: 10.1016/j.resmic.2024.104260

Jinjin Li, Jiaojiao Li, Peixian Li, Jie Zhang, Qian Liu, Hao Qi

{"title":"Influence of 5'-UTR nucleotide composition on translation efficiency in Escherichiacoli.","authors":"Jinjin Li, Jiaojiao Li, Peixian Li, Jie Zhang, Qian Liu, Hao Qi","doi":"10.1016/j.resmic.2024.104260","DOIUrl":"10.1016/j.resmic.2024.104260","url":null,"abstract":"<p><p>Translation initiation for 5'-UTR contributes primarily to the efficient protein expression in Escherichia coli. Many studies have focused on constructing random 5'-UTR libraries to investigate the impact of mRNA features on protein translation efficiency. However, the study on the effect of the absence of specific types of nucleotides in the entire 5'-UTR region on translation efficiency has not yet been reported. Here, we constructed four reporter plasmid libraries encoding the sfGFP fluorescent protein, each preceded by 5'-UTRs that lack one specific nucleotide (25B, 25D, 25H, 25V). Each library was transformed into E. coli cells, and the fluorescence distribution among the different libraries was analyzed by flow cytometer. Additionally, we quantified the activity of 256 unique 5'-UTR sequences and analyzed the impact of the corresponding mRNA sequence features on translation efficiency. We found that the 25D library, which lacks the C nucleotide, exhibited the highest overall translation efficiency compared to the other three libraries. Moreover, the minimum free energy and 16S rRNA hybridization energy of the 5'-UTR sequence could work coordinately to influence translation efficiency. The 5'-UTR sequences lacking the C nucleotide also achieve efficient protein translation. These findings may provide several guiding principles for precisely tuning protein expression.</p>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":" ","pages":"104260"},"PeriodicalIF":2.5,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting MurG enzyme in Klebsiella pneumoniae: An in silico approach to novel antimicrobial discovery. 以肺炎克雷伯氏菌中的 MurG 酶为靶标：新型抗菌药发现的硅学方法。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-11-06 DOI: 10.1016/j.resmic.2024.104257

Pragati Mahur, Amit Kumar Singh, Jayaraman Muthukumaran, Monika Jain

{"title":"Targeting MurG enzyme in Klebsiella pneumoniae: An in silico approach to novel antimicrobial discovery.","authors":"Pragati Mahur, Amit Kumar Singh, Jayaraman Muthukumaran, Monika Jain","doi":"10.1016/j.resmic.2024.104257","DOIUrl":"https://doi.org/10.1016/j.resmic.2024.104257","url":null,"abstract":"<p><p>Antibiotic resistance poses a global crisis fuelled by widespread antibiotic use, particularly against Gram-negative bacteria like Klebsiella pneumoniae, a leading cause of hospital-acquired infections with high mortality rates. Urgent identification of effective drug targets is imperative, with a focus on metabolic pathways to inhibit bacterial growth. Targeting the crucial metabolic pathways of K. pneumoniae would be a more efficient way to prevent its growth and the diseases that it causes. The present study focused on inhibiting the UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (MurG) enzyme, which is a key enzyme in peptidoglycan biosynthesis pathway. A high throughput virtual screening was used to find possible lead molecules from Enamine -High-Throughput Screening Center library. The resulting high binding affinity ligands were further assessed for their drug-likeness and other pharmacokinetic properties. Based on these analyses, the three ligands Z95813755_1, Z324718246_1 and Z324718246_2 were selected for further molecular dynamic simulation studies. The molecular dynamic simulation results and MM/PBSA analysis predicted that both Z95813755_1 and Z324718246_2, molecules show higher binding affinity towards MurG. For the first time we are reporting potential candidate inhibitors against MurG from K. pneumoniae, providing new insights in management of multi drug resistant K. pneumoniae infections.</p>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":" ","pages":"104257"},"PeriodicalIF":2.5,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolutionary trends indicate a coherent organization of sap operons 进化趋势表明，树液操作子的组织是一致的。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-11-01 DOI: 10.1016/j.resmic.2024.104228

Pratik Dasgupta, Kavya Vinil, Shankar Prasad Kanaujia

{"title":"Evolutionary trends indicate a coherent organization of sap operons","authors":"Pratik Dasgupta, Kavya Vinil, Shankar Prasad Kanaujia","doi":"10.1016/j.resmic.2024.104228","DOIUrl":"10.1016/j.resmic.2024.104228","url":null,"abstract":"<div><div><span>Human hosts possess a complex network of immune responses against microbial pathogens. The production of antimicrobial peptides (AMPs), which target the pathogen cell membranes and inhibit them from inhabiting the hosts, is one such mechanism. However, pathogens have evolved systems that encounter these host-produced AMPs. The Sap (</span><u>s</u>ensitivity to <u>a</u>ntimicrobial <u>p</u>eptides) transporter uptakes AMPs inside the microbial cell and proteolytically degrades them. The Sap transporters comprise five subunits encoded by genes in an operon. Despite its ubiquitous nature, its subunits are not found to be in tandem with many organisms. In this study, a total of 421 Sap transporters were analyzed for their operonic arrangement. Out of 421, a total of 352 operons were found to be in consensus arrangement, while the remaining 69 show a varying arrangement of genes. The analysis of the intergenic distance between the subunits of the <em>sap</em> operon suggests a signature pattern with <em>sapAB</em> (-4), <em>sapBC</em> (-14), <em>sapCD</em> (-1), and <em>sapDF</em><span> (-4 to 1). An evolutionary analysis of these operons favors the consensus arrangement of the Sap transporter systems, substantiating its prevalence in most of the Gram-negative pathogens. Overall, this study provides insight into bacterial evolution, favoring the maintenance of the genetic organization of essential pathogenicity factors.</span></div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 8","pages":"Article 104228"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthetic capabilities of Antarctic yeast Sporobolomyces roseus AL103: Temperature influence on intracellular metabolites and characterization of the exopolysaccharide 南极酵母 Sporobolomyces roseus AL103 的生物合成能力：温度对胞内代谢物的影响和外多糖的特征。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-11-01 DOI: 10.1016/j.resmic.2024.104247

Snezhana Rusinova-Videva , Manol Ognyanov , Kalina Alipieva , Stefka Nachkova , Dessislava Gerginova , Ani Petrova , Maria Marudova , Sofia Milenkova , Tsvetelina Paunova-Krasteva , Dragomir Mateev

{"title":"Biosynthetic capabilities of Antarctic yeast Sporobolomyces roseus AL103: Temperature influence on intracellular metabolites and characterization of the exopolysaccharide","authors":"Snezhana Rusinova-Videva , Manol Ognyanov , Kalina Alipieva , Stefka Nachkova , Dessislava Gerginova , Ani Petrova , Maria Marudova , Sofia Milenkova , Tsvetelina Paunova-Krasteva , Dragomir Mateev","doi":"10.1016/j.resmic.2024.104247","DOIUrl":"10.1016/j.resmic.2024.104247","url":null,"abstract":"<div><div>The purpose of the study was to investigate the biosynthetic properties of the Antarctic yeast strain <em>Sporobolomyces roseus</em> AL<sub>103</sub> in response to temperature changes, to perform intracellular metabolic profiling, and to reveal the chemical and functional characteristics of the synthesized exopolysaccharide (EPS). The results show that the yeast strain needed a shorter time to reach a stationary phase at 22 °C contrary to that of 5 °C. An NMR analysis revealed differences in metabolic profiles of amino acids, glucose, trehalose, glycerol, uridine, etc. EPS (2.9 g/L) was characterized by high–molecular-weight with total carbohydrate, uronic acids, and protein content of 66 %, 10.5 %, and 2.5 %, respectively. Mannose (74 mol%) and galactose (19 mol%) were the major constituents. The FT-IR data suggested the presence of β-(1–4)-mannan. DSC thermogram, WVTR, mechanical properties, and moisture sorption of the EPS film showed thermal stability up to 220 °C and hydrophilic behavior. The newly obtained polymer film was studied for the first time and the data showed possibilities for its successful application as a film-forming material in the preparation of packaging materials. In conclusion, the temperature influenced the metabolic profile of the Antarctic yeast producer. The biotechnological process could be directed to obtain the target intracellular or extracellular metabolites.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 8","pages":"Article 104247"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0