Research in microbiology最新文献

Transposition of transposable element IS1 in Edwardsiella piscicida mutant generated by CRISPR/Cas9 along with λ-Red recombineering system.

IF 2.5 4区生物学

Research in microbiology Pub Date : 2025-04-02 DOI: 10.1016/j.resmic.2025.104297

Eun Gyeong Lee, Ki Hong Kim

{"title":"Transposition of transposable element IS1 in Edwardsiella piscicida mutant generated by CRISPR/Cas9 along with λ-Red recombineering system.","authors":"Eun Gyeong Lee, Ki Hong Kim","doi":"10.1016/j.resmic.2025.104297","DOIUrl":"10.1016/j.resmic.2025.104297","url":null,"abstract":"This study aimed to investigate unintended mutations introduced by the CRISPR/Cas9 genome editing system in Edwardsiella piscicida. Whole-genome sequencing was conducted on the wild-type E. piscicida NH1 and its alanine racemase knockout mutants (E. piscicida Δalr325 NH1 and E. piscicida Δalr50 NH1) generated using CRISPR/Cas9 with a λ-Red recombineering system. Comparative genomic analyses revealed that the insertion sequence 1 (IS1) transpositions occurred in the CRISPR/Cas9-edited mutants, disrupting the type I restriction-modification system subunit M gene, in addition to the targeted gene deletion. Interestingly, no IS1 transpositions were detected in mutants produced via conventional plasmid-based allelic exchange, indicating the potential link between CRISPR/Cas9-mediated editing and transposition events. These results suggest that genome editing via CRISPR/Cas9 could trigger IS1 transposition, potentially due to double-stranded DNA breaks. The lack of sequence similarity between the single guide RNA (sgRNA) and the transposed regions suggests that transpositions are not CRISPR/Cas9 off-target effects. This study provides evidence of interactions between mobile genetic elements and genome editing systems, requiring further investigation into their underlying mechanisms.","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":" ","pages":"104297"},"PeriodicalIF":2.5,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional diversity of AI-2/LuxS system in lactic acid bacteria: Impacts on biofilm formation and environmental resilience.

IF 2.5 4区生物学

Research in microbiology Pub Date : 2025-03-22 DOI: 10.1016/j.resmic.2025.104296

Hopeful Tusalifye Kanthenga, Riza Jane S Banicod, Wilson Ntege, Moses Njeru Njiru, Aqib Javaid, Nazia Tabassum, Young-Mog Kim, Fazlurrahman Khan

引用次数: 0

MibR and LibR are involved in the transcriptional regulation of the ipdC gene in Azospirillum brasilense Sp7.

IF 2.5 4区生物学

Research in microbiology Pub Date : 2025-03-22 DOI: 10.1016/j.resmic.2025.104295

Sandra R Reyes-Carmona, Saúl Jijón Moreno, Alberto Ramírez-Mata, María Luisa Xiqui Vázquez, Beatriz Eugenia Baca

{"title":"MibR and LibR are involved in the transcriptional regulation of the ipdC gene in Azospirillum brasilense Sp7.","authors":"Sandra R Reyes-Carmona, Saúl Jijón Moreno, Alberto Ramírez-Mata, María Luisa Xiqui Vázquez, Beatriz Eugenia Baca","doi":"10.1016/j.resmic.2025.104295","DOIUrl":"https://doi.org/10.1016/j.resmic.2025.104295","url":null,"abstract":"Azospirillum brasilense is a PGPR that produces the phytohormone IAA, a signaling molecule involved in bacteria-plant interaction processes. IAA biosynthesis in Azospirillum is mainly carried out via the IPyA pathway in which the enzyme phenylpyruvate decarboxylase encoded by the ipdC gene is the main. The promoter region of ipdC gene contains cis elements that are highly conserved among different Azospirillum strains. In this work, we identified two proteins that interact with the promoter region of the ipdC gene, named MibR and LibR that belong to the MarR and LuxR transcriptional regulators family, respectively. Both proteins have an HTH domain, and in the case of LibR, it has a REC domain, with aspartic acid residue conserved in positions 7, 8 and 54, this last as a possible phosphorylation target. To explore their participation in the regulation of the ipdC gene, mutants of libR, mibR, and libR-mibR double mutant were generated. The results showed a decrease in IAA biosynthesis that was related to the observed decrease in ipdC gene expression mostly in the doble mutant compared with the wild type. In this work we suggest that ipdC transcription is regulated by LibR and MibR, providing new findings insight into the mechanism employed by A. brasilense to control IAA biosynthesis.","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":" ","pages":"104295"},"PeriodicalIF":2.5,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exacerbation of virulence of multi-drug resistant Escherichia coli O104:H4 by subinhibitory concentrations of ampicillin 氨苄西林亚抑制浓度加重多重耐药大肠杆菌O104:H4的毒力。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2025-03-01 DOI: 10.1016/j.resmic.2025.104266

Yaraymi Ortiz, Vianey Lechuga, Carolina Ortiz, Eduardo Palomino, Eduardo Franco, Norma Heredia, Santos García

{"title":"Exacerbation of virulence of multi-drug resistant Escherichia coli O104:H4 by subinhibitory concentrations of ampicillin","authors":"Yaraymi Ortiz, Vianey Lechuga, Carolina Ortiz, Eduardo Palomino, Eduardo Franco, Norma Heredia, Santos García","doi":"10.1016/j.resmic.2025.104266","DOIUrl":"10.1016/j.resmic.2025.104266","url":null,"abstract":"<div><div>Little is known about how subinhibitory concentrations of antibiotics to which bacteria are resistant affect bacterial virulence. In this study, the effect of subinhibitory concentrations of ampicillin on the virulence of E. coli O104:H4 was analyzed. Bacteria were pre-exposed to 0.1, 0.3, or 0.5 mg/mL of ampicillin in LB media and incubated for 4 h at 37 °C. Transformation capacity (using plasmids and PCR-amplified DNA sequences), swarming motility, biofilm production, curli formation, and virulence gene expression were determined. Ampicillin increased the transformation of E. coli O104:H4, with the highest number of transformants (>104 CFU/ng DNA; p ≤ 0.05) detected after exposure to DNA sequences of spectinomycin. In addition, bacteria pre-treated with 0.5 mg/mL of ampicillin exhibited higher swarming motility (7.6 cm, vs 6.0 cm for control; p ≤ 0.05) and biofilm production (up to 1.9-fold; p ≤ 0.05) when subsequently exposed to 0.1 and 0.3 mg/mL of antibiotic compared with the control. Also, significant overexpression of the virulence-related genes flhC (≤16.1-fold), fliA (≤22.1-fold), csgA (≤3.6-fold), csgD (≤9.1-fold), stx2a (≤32.2-fold), and the antibiotic resistance gene blaTEM-1 (≤5.5-fold) was observed. In conclusion, ampicillin-resistant E. coli O104:H4 increased the expression of its virulence factors when exposed to most subinhibitory concentrations of ampicillin analyzed in this study.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"176 3","pages":"Article 104266"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142966467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cutibacterium acnes and its complex host interaction in prosthetic joint infection: Current insights and future directions 痤疮表皮杆菌及其复杂的宿主相互作用在假体关节感染：目前的见解和未来的方向。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2025-03-01 DOI: 10.1016/j.resmic.2024.104265

Léa Thoraval , Jennifer Varin-Simon , Xavier Ohl , Frédéric Velard , Fany Reffuveille , Min Tang-Fichaux

引用次数: 0

Staphylococcus aureus: Dynamics of pathogenicity and antimicrobial-resistance in hospital and community environments - Comprehensive overview 金黄色葡萄球菌：医院和社区环境中致病性和抗菌药耐药性的动态 - 综合概述。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2025-03-01 DOI: 10.1016/j.resmic.2025.104267

Giorgio Silva-Santana

引用次数: 0

Actinomycetes studies in Tunisia 突尼斯放线菌研究。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2025-03-01 DOI: 10.1016/j.resmic.2025.104279

Souleima Dhahbi, Jeonghee Lee, Dohee Ryu, Ganiyu Akinniyi, Inho Yang

引用次数: 0

Characterization and identification of Pseudomonas sp. AW4, an arsenic-resistant and plant growth-promoting bacteria isolated from the soybean (Glycine max L.) rhizosphere 大豆（Glycine max L.）根际抗砷促进植物生长细菌Pseudomonas sp. AW4的鉴定与鉴定

IF 2.5 4区生物学

Research in microbiology Pub Date : 2025-03-01 DOI: 10.1016/j.resmic.2024.104263

Ana L. Wevar Oller , Gonzalo Torres Tejerizo , Paola P. Pereira , Romina del Pilar Pramparo , Elizabeth Agostini

{"title":"Characterization and identification of Pseudomonas sp. AW4, an arsenic-resistant and plant growth-promoting bacteria isolated from the soybean (Glycine max L.) rhizosphere","authors":"Ana L. Wevar Oller , Gonzalo Torres Tejerizo , Paola P. Pereira , Romina del Pilar Pramparo , Elizabeth Agostini","doi":"10.1016/j.resmic.2024.104263","DOIUrl":"10.1016/j.resmic.2024.104263","url":null,"abstract":"<div><div>Pseudomonas sp. AW4 is a highly arsenic (As) resistant bacterium with plant growth promoting properties, originally isolated from the soybean (Glycine max L.) rhizosphere. In order to safely use this isolate in diverse bioformulations, its characterization needs to be completed and a reliable identification must be provided. In the present work, we analyzed the morpho-physiological, biochemical and genomic characteristics of Pseudomonas sp. AW4. Identification of the isolate varied according to the parameters analyzed, mainly biochemical and physiological tests or individual genes and phylogenetic analyses. In this regard, we performed massive sequencing of its genome, in order to consistently complete its characterization and identification. Pseudomonas sp. AW4 formed a monophyletic clade with P. urmiensis SWRI10, presenting 3.08 % of unique genes against this reference isolate. More than 70 % of AW4 genes were also shared with P. oryziphila strain 1257 NZ and with P. reidholzensis strain CCOS 865. The search for genes related to As resistance evidenced the presence of the operon arsHRBC. Taken together, results of the present work allow identification of this bacterium as Pseudomonas urmiensis AW4 and open up a number of opportunities to study this strain and understand the mechanisms of arsenic resistance and plant growth promotion.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"176 3","pages":"Article 104263"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142795021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel insights into Bacillus thuringiensis: Beyond its role as a bioinsecticide 苏云金芽孢杆菌的新见解：超越其作为生物杀虫剂的作用。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2025-03-01 DOI: 10.1016/j.resmic.2024.104264

Gholamreza Salehi Jouzani , Reza Sharafi , Leandris Argentel-Martínez , Ofelda Peñuelas-Rubio , Ceyda Ozkan , Bengisu Incegul , Rana Goksu , Zehra Hayta , Deniz Yilki , Beyza Yazici , Vildan Hancer , Estibaliz Sansinenea , Jae-Ho Shin , A. El-Shabasy , Ugur Azizoglu

引用次数: 0

Navigating dual-species fungal biofilms: The competitive and cooperative dynamics of Candida albicans 导航双种真菌生物膜：念珠菌的竞争与合作动态。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2025-03-01 DOI: 10.1016/j.resmic.2024.104262

Ouassila Bekkal Brikci-Benhabib

引用次数: 0