Research in microbiology最新文献

BSC2 modulates AmB resistance via the maintenance of intracellular sodium/potassium ion homeostasis in Saccharomyces cerevisiae. BSC2 通过维持酿酒酵母细胞内钠/钾离子平衡调节对 AmB 的抗性

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-09-07 DOI: 10.1016/j.resmic.2024.104245

Zhiwei Huang, Fulong Xiao, Qiao Wang, Xiaojuan Zhang, Yuhu Shen, Yunxia Deng, Ping Shi

{"title":"BSC2 modulates AmB resistance via the maintenance of intracellular sodium/potassium ion homeostasis in Saccharomyces cerevisiae.","authors":"Zhiwei Huang, Fulong Xiao, Qiao Wang, Xiaojuan Zhang, Yuhu Shen, Yunxia Deng, Ping Shi","doi":"10.1016/j.resmic.2024.104245","DOIUrl":"10.1016/j.resmic.2024.104245","url":null,"abstract":"Previous studies on BSC2 have shown that it enhances yeast cell resistance to AmB via antioxidation and induces multidrug resistance by contributing to biofilm formation. Herein, we found that BSC2 overexpression could reverse the sensitivity of pmp3Δ to AmB and help the tested strains restore the intracellular sodium/potassium balance under exposure to AmB. Meanwhile, overexpression of the chitin gene CHS2 could simulate BSC2 to reverse the sensitivity of pmp3Δ and nha1Δ to high salt or AmB. However, BSC2 overexpression in flo11Δ failed to induce AmB resistance, form biofilms, and affect cell wall biogenesis, while CHS2 overexpression compensated the resistance of flo11Δ to AmB. Additionally, BSC2 levels were positively correlated with maintaining cell membrane integrity under exposure to AmB, CAS, or a combination of both. BSC2 overexpression in nha1Δ exhibited a similar function of CHS2, which can compensate for the sensitivity of the mutant to high salt. Altogether, the results demonstrate for the first time that BSC2 may promote ion equilibrium by strengthening cell walls and inhibiting membrane damage in a FLO path-dependent manner, thus enhancing the resistance of yeast cells to AmB. This study also reveals the possible mechanism of antifungal drugs CAS and AmB combined to inhibit fungi.","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142154911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Roles of Pseudomonas aeruginosa siderophores in interaction with prokaryotic and eukaryotic organisms 铜绿假单胞菌苷元在与原核生物和真核生物相互作用中的作用。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-09-01 DOI: 10.1016/j.resmic.2024.104211

{"title":"Roles of Pseudomonas aeruginosa siderophores in interaction with prokaryotic and eukaryotic organisms","authors":"","doi":"10.1016/j.resmic.2024.104211","DOIUrl":"10.1016/j.resmic.2024.104211","url":null,"abstract":"<div>Pseudomonas aeruginosa is an opportunistic pathogen that produces two types of siderophores, pyoverdine and pyochelin, that play pivotal roles in iron scavenging from the environment and host cells. P. aeruginosa siderophores can serve as virulence factors and perform various functions. Several bacterial and fungal species are likely to interact with P. aeruginosa due to its ubiquity in soil and water as well as its potential to cause infections in plants, animals, and humans. Siderophores produced by P. aeruginosa play critical roles in iron scavenging for prokaryotic species (bacteria) and eukaryotic hosts (fungi, animals, insects, invertebrates, and plants) as well. This review provides a comprehensive discussion of the role of P. aeruginosa siderophores in interaction with prokaryotes and eukaryotes as well as their underlying mechanisms of action. The evolutionary relationship between P. aeruginosa siderophore recognition receptors, such as FpvA, FpvB, and FptA, and those of other bacterial species has also been investigated.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140909218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of β-ionone on bacterial cells: the use of specific lux-biosensors β-酮对细菌细胞的影响：特定勒克斯生物传感器的使用。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-09-01 DOI: 10.1016/j.resmic.2024.104214

{"title":"The effect of β-ionone on bacterial cells: the use of specific lux-biosensors","authors":"","doi":"10.1016/j.resmic.2024.104214","DOIUrl":"10.1016/j.resmic.2024.104214","url":null,"abstract":"<div>The diversity of the biological activity of volatile organic compounds (VOCs), including unsaturated ketone β-ionone, promising pharmacological, biotechnological, and agricultural agent, has aroused considerable interest. However, the functional role and mechanisms of action of VOCs remain insufficiently studied. In this work, the response of bacterial cells to the action of β-ionone was studied using specific bioluminescent lux-biosensors containing stress-sensitive promoters. We determined that in Escherichia coli cells, β-ionone induces oxidative stress (PkatG and Pdps promoters) through a specific response mediated by the OxyR/OxyS regulon, but not SoxR/SoxS (PsoxS promoter). It has been shown that β-ionone at high concentrations (50 μM and above) causes a weak induction of the expression from the PibpA promoter and slightly induces the PcolD promoter in the E. coli biosensors; the observed effect is enhanced in the ΔoxyR mutants. This indicates the presence of some damage to proteins and DNA. β-Ionone was found to inhibit the bichaperone-dependent DnaKJE-ClpB refolding of heat-inactivated bacterial luciferase in E. coli wild-type and ΔibpB mutant strains. In the cells of the Gram-positive bacterium Bacillus subtilis 168 pNK-MrgA β-ionone does not cause oxidative stress. Thus, in this work, the specificity of bacterial cell stress responses to the action of β-ionone was shown.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140916647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular insights into PGPR fluorescent Pseudomonads complex mediated intercellular and interkingdom signal transduction mechanisms in promoting plant's immunity PGPR荧光假单胞菌复合体介导的细胞间和王国间信号转导机制在促进植物免疫方面的分子见解。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-09-01 DOI: 10.1016/j.resmic.2024.104218

引用次数: 0

Metagenomics approaches in unveiling the dynamics of Plant Growth-Promoting Microorganisms (PGPM) vis-à-vis Phytophthora sp. suppression in various crop ecological systems 元基因组学方法揭示了各种作物生态系统中植物生长促进微生物 (PGPM) 与抑制疫霉菌 (Phytophthora sp.) 的动态关系。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-09-01 DOI: 10.1016/j.resmic.2024.104217

引用次数: 0

Listeria monocytogenes in beef: a hidden risk 牛肉中的李斯特菌：隐藏的风险

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-09-01 DOI: 10.1016/j.resmic.2024.104215

引用次数: 0

d-aspartate, an amino-acid important for human health, supports anaerobic respiration in several Campylobacter species D- 天门冬氨酸是一种对人体健康非常重要的氨基酸，它支持弯曲杆菌中多种细菌的厌氧呼吸。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-09-01 DOI: 10.1016/j.resmic.2024.104219

{"title":"d-aspartate, an amino-acid important for human health, supports anaerobic respiration in several Campylobacter species","authors":"","doi":"10.1016/j.resmic.2024.104219","DOIUrl":"10.1016/j.resmic.2024.104219","url":null,"abstract":"<div>Despite being classified as microaerophilic microorganisms, most Campylobacter species can grow anaerobically, using formate or molecular hydrogen (H2) as electron donors, and various nitrogenous and sulfurous compounds as electron acceptors. Herein, we showed that both l-asparagine (l-Asn) and l-aspartic acid (l-Asp) bolster H2-driven anaerobic growth in several Campylobacter species, whereas the d-enantiomer form of both asparagine (d-Asn) and aspartic acid (d-Asp) only increased anaerobic growth in Campylobacter concisus strain 13826 and Campylobacter ureolyticus strain NCTC10941. A gene annotated as racD encoding for a putative d/l-Asp racemase was identified in the genome of both strains. Disruption of racD in Cc13826 resulted in the inability of the mutant strain to use either d-enantiomer during anaerobic growth. Hence, our results suggest that the racD gene is required for campylobacters to use either d-Asp or d-Asn. The use of d-Asp by various human opportunistic bacterial pathogens, including C. concisus, C. ureolyticus, and also possibly select strains of Campylobacter gracilis, Campylobacter rectus and Campylobacter showae, is significant, because d-Asp is an important signal molecule for both human nervous and neuroendocrine systems. To our knowledge, this is the first report of pathogens scavenging a d-amino acid essential for human health.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141470480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endopeptidase activities of Clostridium botulinum toxins in the development of this bacterium 肉毒梭状芽孢杆菌毒素在该细菌发展过程中的内肽酶活性。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-09-01 DOI: 10.1016/j.resmic.2024.104216

引用次数: 0

Biofilms in soils: The evidence about sessile versus planktonic microorganisms needs revisiting 土壤中的生物膜：需要重新审视有关无柄微生物与浮游微生物的证据。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-09-01 DOI: 10.1016/j.resmic.2024.104204

引用次数: 0

Lipid lysination by MprF contributes to hemolytic pigment retention in group B Streptococcus. MprF 的脂质裂解作用有助于 B 群链球菌中溶血性色素的保留。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-08-26 DOI: 10.1016/j.resmic.2024.104231

Elise Caliot, Arnaud Firon, Audrey Solgadi, Patrick Trieu-Cuot, Shaynoor Dramsi

引用次数: 0