Research in microbiology最新文献

{"title":"Transcriptome analysis during thermal-induced endogenous mutagenesis in a strict psychrotrophic Aeromonas salmonicida subp. salmonicida strain.","authors":"Javier Santander, Ahmed Hossain, Katherine Valderrama, Jules Hermet, Trung Cao, Oluwatoyin Onireti, Ignacio Vasquez, Hajarooba Gnanagobal","doi":"10.1016/j.resmic.2025.104305","DOIUrl":"10.1016/j.resmic.2025.104305","url":null,"abstract":"<p><p>Aeromonas salmonicida is one of the earliest identified fish pathogens and the causative agent of furunculosis. A. salmonicida can be cultivated at temperatures as high as 30 °C, but at temperatures over 24 °C, insertion sequence (IS) elements cause endogenous mutagenesis. Here, we used a phenotypical and transcriptomics analysis to study the molecular aspects of A. salmonicida endogenous mutagenesis. Endogenous mutagenesis became evident after 12 h post-thermal induction and reached the highest level after 24 h. RNA was extracted from A. salmonicida grown at 15 and 28 °C after 16 h. Differential gene expression analysis showed that 344 differential expressed genes were up-regulated and 364 were down-regulated at 28 °C. Several ISs were upregulated in the chromosome and virulence plasmid. Virulence genes, including the type 3 secretion system, A-layer, and melanin synthesis, were downregulated. Overall, our result indicates that A. salmonicida incubated at 28 °C was under moderate heat stress but under strong stress caused by ISs mutagenesis. These results align with the hypothesis that ISs constrain virulent A. salmonicida to a psychrotrophic lifestyle, potentially transitioning to an attenuated mesophilic lifestyle.</p>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":" ","pages":"104305"},"PeriodicalIF":2.5,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-drug resistance and diversity of mobile genetic elements in Escherichia coli isolated from migratory bird in Poyang Lake.","authors":"Haixin Deng, Huiping Liang, Qinghua Zeng, Peng Huang, Hui Yan, Huabin Cao, Huansheng Wu","doi":"10.1016/j.resmic.2025.104306","DOIUrl":"10.1016/j.resmic.2025.104306","url":null,"abstract":"<p><p>With the spread of antibiotic resistance genes such as blaCTX-M-2, dfrA1 and blaNDM-1, the problem of drug resistance in E. coli is becoming increasingly serious [1]. This study aimed to identify integrons genes and MGEs in E. coli isolated from migratory birds' feces at Poyang Lake, Jiangxi Province, focusing on their role in antimicrobial resistance (AMR). The 114 isolated E. coli strains were tested by standard disk diffusion method and genetic testing method. Results showed 64.04 % (73/114) of isolates were multi-drug resistance (MDR), mainly resistant to 3-6 antibiotics. Common resistances included neomycin (50 %) and streptomycin (48.25 %). We detected 21 mobile genetic elements, including IS903 (92.11 %), traA (72.81 %), ISCR3 (64.91 %), and ISpa7 (50 %). These elements were present in all isolates, forming 112 combinations. Significant differences in resistance rates were found between class I integron-positive and negative strains for doxycycline, tetracycline, bacitracin, and streptomycin (P < 0.01), and for neomycin (P < 0.05). Class II integron-positive bacteria showed higher resistance to doxycycline (P < 0.01) and ceftizoxime (P < 0.05). No significant differences were observed for class III integron-positive strains. This study underscores the prevalence of multidrug-resistant and the diversity of mobile genetic elements in E. coli, emphasizing the need for continuous monitoring.</p>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":" ","pages":"104306"},"PeriodicalIF":2.5,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144619949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional evaluation of functional genes of resistance, biofilm and quorum sensing in CTX-M15 ESBL-producing Klebsiella pneumoniae after meropenem concentration based on serum level.","authors":"Suzana Carstensen, Paula Hansen Suss, Gabriel Burato Ortis, Rita Estrela, Edlaine Rijo Costa, Fernanda L Moreira, Thalissa Colodiano Martins, Joao Paulo Telles, Felipe Francisco Tuon","doi":"10.1016/j.resmic.2025.104304","DOIUrl":"10.1016/j.resmic.2025.104304","url":null,"abstract":"<p><strong>Background: </strong>Klebsiella pneumoniae is a leading cause of multidrug-resistant hospital-acquired infections. Resistance to carbapenems, particularly meropenem, is increasingly reported and often linked to β-lactamase production, porin alterations, and efflux pump overexpression. However, the immediate transcriptional response of clinical isolates to meropenem remains poorly characterized.</p><p><strong>Aim: </strong>To investigate the transcriptomic response of a CTX-M-15-producing K. pneumoniae clinical isolate to clinically relevant meropenem exposure and identify potential non-enzymatic resistance and survival mechanisms.</p><p><strong>Methods: </strong>A meropenem-susceptible K. pneumoniae isolate was recovered from a bacteremic patient and confirmed to carry CTX-M-15, SHV-182, and OXA-1 β-lactamase genes. Time-kill assays were performed using serum-level meropenem concentrations. RNA was extracted at multiple time points (0, 10, 30, and 60 min) post-exposure, followed by RNA-sequencing. Differential gene expression was analyzed using DESeq2. Resistance genes were identified via genome sequencing and annotated using CARD and VFDB databases.</p><p><strong>Results: </strong>Meropenem exhibited a concentration-dependent bactericidal effect, with full inhibition sustained only at serum-level concentrations. Transcriptomic analysis revealed significant upregulation of genes linked to biofilm formation (e.g., osmB, lipoproteins), efflux pumps (mepA, acrB), and cell wall remodeling (murJ, lpoB). No differential expression was observed for blaCTX-M-15, blaOXA-1, or blaSHV-182. The transcriptional regulator ompR was induced, suggesting membrane permeability adjustments.</p><p><strong>Conclusion: </strong>K. pneumoniae rapidly activates adaptive stress responses under meropenem exposure, primarily through biofilm-related genes, efflux pumps, and membrane remodeling rather than increased β-lactamase expression. These findings underscore the complexity of antimicrobial tolerance mechanisms and may inform novel therapeutic strategies targeting transcriptional plasticity in multidrug-resistant pathogens.</p>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":" ","pages":"104304"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Pseudomonas aeruginosa ExoU on airway epithelial cells through MUC1-C nuclear translocation","authors":"Alexandre Costa Monteiro ,&nbsp;Karen Rosalino Emmerick ,&nbsp;Lhousseine Touqui ,&nbsp;Alessandra Mattos Saliba","doi":"10.1016/j.resmic.2025.104301","DOIUrl":"10.1016/j.resmic.2025.104301","url":null,"abstract":"<div><div>The opportunistic pathogen <em>Pseudomonas aeruginosa</em> plays a significant role in hospital-acquired pneumonia, with the secretion of ExoU - a virulence factor expressed by select <em>P. aeruginosa</em> strains - linked to poor clinical outcomes. This is due to ExoU's phospholipase A₂ activity, which triggers an uncontrolled inflammatory response, leading to severe pathologies. Here, we delve into ExoU's impact on mucin-1 (MUC1), a critical mucus component with immunomodulatory properties on pulmonary cell surfaces. Our findings reveal that infection of human respiratory epithelial cells by an ExoU-negative <em>P. aeruginosa</em> strain boosts MUC1 expression, yet ExoU secretion by the bacteria diminishes MUC1 mRNA and protein levels through JNK pathway disruption. Interestingly, despite reduced overall cell-associated MUC1 levels, ExoU prompts MUC1 accumulation within the nucleus, where the MUC1 cytoplasmic tail (MUC1-C) may act as a transcriptional coactivator. Notably, treating epithelial respiratory cell cultures with GO-201, a specific MUC1-C inhibitor, diminished CXCL-8 secretion induced by ExoU. In essence, our study highlights how infection by ExoU-producing <em>P. aeruginosa</em> strains suppresses MUC1 expression during infection, while enhancing MUC1-C translocation to the nucleus, where it plays a pro-inflammatory role. This unique mechanism sheds light on how ExoU can impact the host's defense against <em>P. aeruginosa</em>, potentially compromising host health.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"176 5","pages":"Article 104301"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional diversity of AI-2/LuxS system in lactic acid bacteria: Impacts on biofilm formation and environmental resilience","authors":"Hopeful Tusalifye Kanthenga ,&nbsp;Riza Jane S. Banicod ,&nbsp;Wilson Ntege ,&nbsp;Moses Njeru Njiru ,&nbsp;Aqib Javaid ,&nbsp;Nazia Tabassum ,&nbsp;Young-Mog Kim ,&nbsp;Fazlurrahman Khan","doi":"10.1016/j.resmic.2025.104296","DOIUrl":"10.1016/j.resmic.2025.104296","url":null,"abstract":"<div><div>A key component of microbial communication, autoinducer-2 (AI-2) signaling, affects several physiological processes, including environmental adaptation and biofilm formation in lactic acid bacteria (LAB). The multifarious contribution of AI-2, synthesized by LuxS, in improving biofilms and tolerance to hostile conditions in LAB has been investigated in this review. The evolutionary conservation and diversity of AI-2 are shown by a phylogenetic analysis of <em>luxS</em> gene among several LAB species. Furthermore, AI-2 signaling in LAB improves resistance to unfavorable environmental factors, including pH fluctuations, temperature extremes, and antimicrobial agents. Lactic acid bacteria could set off defenses against harmful impacts from environmental stresses.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"176 5","pages":"Article 104296"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143693125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"2-Chloromethyl anthraquinone inhibits Candida albicans biofilm formation by inhibiting the Ras1-cAMP-Efg1 pathway","authors":"Haoying Zhang,&nbsp;Qi Zhang,&nbsp;Ting Zuo,&nbsp;Ziqi Wang,&nbsp;Jianmin Liao,&nbsp;Yuanyuan Lu","doi":"10.1016/j.resmic.2025.104280","DOIUrl":"10.1016/j.resmic.2025.104280","url":null,"abstract":"<div><div><em>Candida albicans</em> is an opportunistic pathogen, and the formation of its biofilm makes it resistant to traditional antifungal therapy. Anthraquinones have universal antibacterial activity. We evaluated the inhibitory effects of 2-chloromethyl anthraquinone on <em>C. albicans</em> adhesion, mycelial morphology transformation, and biofilm formation. The results showed that 2-chloromethyl anthraquinone could inhibit <em>C. albicans</em> adhesion, mycelium formation, and biofilm formation in a dose-dependent manner at 2 μg/mL. In addition, 2-chloromethyl anthraquinone significantly inhibited the expression of biofilm formation-related genes in <em>C. albicans</em>, including <em>ALS1</em>, <em>CPH1</em>, <em>ECE1</em>, <em>HWP1</em>, <em>TEC1</em>, <em>BCR1</em>, and <em>UME6</em>. In addition, Ras1-cAMP-Efg1 pathway-related genes (<em>RAC1</em>, <em>CYR1</em>, and <em>TPK2</em>) were also significantly down-regulated, indicating that the inhibitory effect of 2-chloromethyl anthraquinone on <em>C. albicans</em> biofilms may be related to the Ras1-cAMP-Efg1 signaling pathway. In summary, the results of this study confirmed the inhibitory mechanism of 2-chloromethyl anthraquinone on the virulence factors of <em>C. albicans</em>, which laid a theoretical foundation for its use as an anti-biofilm agent against <em>C. albicans</em>.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"176 5","pages":"Article 104280"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Switching off the yeast-to-hyphae transition in Yarrowia lipolytica through histone deacetylase inhibitors","authors":"Martha Viviana Roa-Cordero ,&nbsp;Christian Alfonso Arenas-Sepúlveda ,&nbsp;María Cristina Herrera-Plata ,&nbsp;Sandra Milena Leal-Pinto ,&nbsp;Nubia Andrea Villota-Salazar ,&nbsp;Juan Manuel González-Prieto","doi":"10.1016/j.resmic.2025.104299","DOIUrl":"10.1016/j.resmic.2025.104299","url":null,"abstract":"<div><div>Fungi can develop a variety of morphotypes to survive, colonize, adapt and prevail under different environmental conditions. In general, two morphological shapes encompass the others: yeast (unicellular) and hyphae (multicellular). Under specific conditions, some fungi can adopt these two cellular morphologies, and for this reason, they are called dimorphic. Histone acetylation and deacetylation are well-known important mechanisms of chromatin remodelling that control cell differentiation processes as dimorphism. The reactions involved are catalysed by histone acetyltransferases (HATs) and histone deacetylases (HDACs), respectively. In the present work, we used <em>Yarrowia lipolytica</em> as a dimorphic fungal model to investigate the effect of HDAC chemical inhibition on the growth and yeast-to-hyphae switch of fungi. For this purpose, we tested the compounds sodium butyrate (SB) and valproic acid (VPA) as epigenetic modulators. Our results indicated that <em>Y. lipolytica</em> tolerates high doses of these inhibitors due to its lipolytic nature. However, once the metabolic capability of the fungus is overcome, SB and VPA strongly suppress hyphal growth, suggesting that histone acetylation plays a pivotal role in the regulation of this process.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"176 5","pages":"Article 104299"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MibR and LibR are involved in the transcriptional regulation of the ipdC gene in Azospirillum brasilense Sp7","authors":"Sandra R. Reyes-Carmona ,&nbsp;Saúl Jijón Moreno ,&nbsp;Alberto Ramírez-Mata ,&nbsp;María Luisa Xiqui Vázquez ,&nbsp;Beatriz Eugenia Baca","doi":"10.1016/j.resmic.2025.104295","DOIUrl":"10.1016/j.resmic.2025.104295","url":null,"abstract":"<div><div><em>Azospirillum brasilense</em> is a PGPR that produces the phytohormone IAA, a signaling molecule involved in bacteria-plant interaction processes. IAA biosynthesis in <em>Azospirillum</em> is mainly carried out via the IPyA pathway in which the enzyme phenylpyruvate decarboxylase encoded by the <em>ipdC</em> gene is the main. The promoter region of <em>ipdC</em> gene contains <em>cis</em> elements that are highly conserved among different <em>Azospirillum</em> strains. In this work, we identified two proteins that interact with the promoter region of the <em>ipdC</em> gene, named MibR and LibR that belong to the MarR and LuxR transcriptional regulators family, respectively. Both proteins have an HTH domain, and in the case of LibR, it has a REC domain, with aspartic acid residue conserved in positions 7, 8 and 54, this last as a possible phosphorylation target. To explore their participation in the regulation of the <em>ipdC</em> gene, mutants of <em>libR</em>, <em>mibR</em>, and <em>libR-mibR</em> double mutant were generated. The results showed a decrease in IAA biosynthesis that was related to the observed decrease in <em>ipdC</em> gene expression mostly in the doble mutant compared with the wild type. In this work we suggest that <em>ipdC</em> transcription is regulated by LibR and MibR, providing new findings insight into the mechanism employed by <em>A. brasilense</em> to control IAA biosynthesis.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"176 5","pages":"Article 104295"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial resistance in coagulase negative staphylococci: Genome analysis and role of horizontal gene transfer","authors":"Tansu Dündar,&nbsp;Fatma Köksal Çakırlar","doi":"10.1016/j.resmic.2025.104298","DOIUrl":"10.1016/j.resmic.2025.104298","url":null,"abstract":"<div><div>Coagulase-negative staphylococci (CNS) are emerging as significant contributors to antimicrobial resistance, yet their genomic characteristics remain incompletely understood. This study presents a whole-genome analysis of 12 multidrug-resistant CNS strains (<em>Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus hominis</em>) isolated from blood cultures, focusing on antimicrobial resistance genes, mobile genetic elements (MGEs), and horizontal gene transfer (HGT) mechanisms.</div><div>We identified 22 resistance genes conferring resistance to 11 antimicrobial classes, many of which were plasmid-associated. Notably, we report the first detection of the ISSha1 insertion sequence in <em>S. hominis</em>, along with novel resistance plasmids, including pGO1 and VRSAp in <em>S. haemolyticus</em> and pAMα1 in <em>S. hominis</em>. The identification of bacteriophage-derived sequences in <em>S. haemolyticus</em> and <em>S. hominis</em> suggests a role for phages in genetic exchange. CRISPR sequences and a Cas gene were detected in <em>S. hominis</em>, suggesting a potential but unconfirmed role in restricting gene transfer. Additionally, pGO1 was identified as a conjugative plasmid, while pAMα1 and VRSAp were determined to be mobilizable, reinforcing the role of CNS in resistance dissemination.</div><div>These results highlight CNS as reservoirs of antimicrobial resistance genes and emphasize the importance of species-specific genomic surveillance. Proactive monitoring of CNS is crucial for controlling antimicrobial resistance in clinical settings.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"176 5","pages":"Article 104298"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transposition of transposable element IS1 in Edwardsiella piscicida mutant generated by CRISPR/Cas9 along with λ-Red recombineering system","authors":"Eun Gyeong Lee,&nbsp;Ki Hong Kim","doi":"10.1016/j.resmic.2025.104297","DOIUrl":"10.1016/j.resmic.2025.104297","url":null,"abstract":"<div><div>This study aimed to investigate unintended mutations introduced by the CRISPR/Cas9 genome editing system in <em>Edwardsiella piscicida</em>. Whole-genome sequencing was conducted on the wild-type <em>E. piscicida NH1</em> and its alanine racemase knockout mutants (<em>E. piscicida Δalr325 NH1</em> and <em>E. piscicida Δalr50 NH1</em>) generated using CRISPR/Cas9 with a λ-Red recombineering system. Comparative genomic analyses revealed that the insertion sequence 1 (IS1) transpositions occurred in the CRISPR/Cas9-edited mutants, disrupting the type I restriction-modification system subunit M gene, in addition to the targeted gene deletion. Interestingly, no IS1 transpositions were detected in mutants produced via conventional plasmid-based allelic exchange, indicating the potential link between CRISPR/Cas9-mediated editing and transposition events. These results suggest that genome editing via CRISPR/Cas9 could trigger IS1 transposition, potentially due to double-stranded DNA breaks. The lack of sequence similarity between the single guide RNA (sgRNA) and the transposed regions suggests that transpositions are not CRISPR/Cas9 off-target effects. This study provides evidence of interactions between mobile genetic elements and genome editing systems, requiring further investigation into their underlying mechanisms.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"176 5","pages":"Article 104297"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}