Research in microbiology最新文献

Actinomycetes studies in Tunisia. 突尼斯放线菌研究。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2025-01-17 DOI: 10.1016/j.resmic.2025.104279

Souleima Dhahbi, Jeonghee Lee, Dohee Ryu, Ganiyu Akinniyi, Inho Yang

引用次数: 0

Staphylococcus aureus: Dynamics of pathogenicity and antimicrobial-resistance in hospital and community environments - Comprehensive overview. 金黄色葡萄球菌：医院和社区环境中致病性和抗菌药耐药性的动态 - 综合概述。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2025-01-11 DOI: 10.1016/j.resmic.2025.104267

Giorgio Silva-Santana

引用次数: 0

Exacerbation of virulence of multi-drug resistant Escherichia coli O104:H4 by subinhibitory concentrations of ampicillin. 氨苄西林亚抑制浓度加重多重耐药大肠杆菌O104:H4的毒力。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2025-01-08 DOI: 10.1016/j.resmic.2025.104266

Yaraymi Ortiz, Vianey Lechuga, Carolina Ortiz, Eduardo Palomino, Eduardo Franco, Norma Heredia, Santos García

{"title":"Exacerbation of virulence of multi-drug resistant Escherichia coli O104:H4 by subinhibitory concentrations of ampicillin.","authors":"Yaraymi Ortiz, Vianey Lechuga, Carolina Ortiz, Eduardo Palomino, Eduardo Franco, Norma Heredia, Santos García","doi":"10.1016/j.resmic.2025.104266","DOIUrl":"10.1016/j.resmic.2025.104266","url":null,"abstract":"<p><p>Little is known about how subinhibitory concentrations of antibiotics to which bacteria are resistant affect bacterial virulence. In this study, the effect of subinhibitory concentrations of ampicillin on the virulence of E. coli O104:H4 was analyzed. Bacteria were pre-exposed to 0.1, 0.3, or 0.5 mg/mL of ampicillin in LB media and incubated for 4 h at 37 °C. Transformation capacity (using plasmids and PCR-amplified DNA sequences), swarming motility, biofilm production, curli formation, and virulence gene expression were determined. Ampicillin increased the transformation of E. coli O104:H4, with the highest number of transformants (>10<sup>4</sup> CFU/ng DNA; p ≤ 0.05) detected after exposure to DNA sequences of spectinomycin. In addition, bacteria pre-treated with 0.5 mg/mL of ampicillin exhibited higher swarming motility (7.6 cm, vs 6.0 cm for control; p ≤ 0.05) and biofilm production (up to 1.9-fold; p ≤ 0.05) when subsequently exposed to 0.1 and 0.3 mg/mL of antibiotic compared with the control. Also, significant overexpression of the virulence-related genes flhC (≤16.1-fold), fliA (≤22.1-fold), csgA (≤3.6-fold), csgD (≤9.1-fold), stx2a (≤32.2-fold), and the antibiotic resistance gene blaTEM-1 (≤5.5-fold) was observed. In conclusion, ampicillin-resistant E. coli O104:H4 increased the expression of its virulence factors when exposed to most subinhibitory concentrations of ampicillin analyzed in this study.</p>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":" ","pages":"104266"},"PeriodicalIF":2.5,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142966467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting MurG enzyme in Klebsiella pneumoniae: An in silico approach to novel antimicrobial discovery 以肺炎克雷伯氏菌中的 MurG 酶为靶标：新型抗菌药发现的硅学方法。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2025-01-01 DOI: 10.1016/j.resmic.2024.104257

Pragati Mahur, Amit Kumar Singh, Jayaraman Muthukumaran, Monika Jain

{"title":"Targeting MurG enzyme in Klebsiella pneumoniae: An in silico approach to novel antimicrobial discovery","authors":"Pragati Mahur, Amit Kumar Singh, Jayaraman Muthukumaran, Monika Jain","doi":"10.1016/j.resmic.2024.104257","DOIUrl":"10.1016/j.resmic.2024.104257","url":null,"abstract":"<div><div>Antibiotic resistance poses a global crisis fuelled by widespread antibiotic use, particularly against Gram-negative bacteria like <em>Klebsiella pneumoniae</em>, a leading cause of hospital-acquired infections with high mortality rates. Urgent identification of effective drug targets is imperative, with a focus on metabolic pathways to inhibit bacterial growth. Targeting the crucial metabolic pathways of <em>K. pneumoniae</em> would be a more efficient way to prevent its growth and the diseases that it causes. The present study focused on inhibiting the UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide)pyrophosphoryl-undecaprenol <em>N</em>-acetylglucosamine transferase (MurG) enzyme, which is a key enzyme in peptidoglycan biosynthesis pathway. A high throughput virtual screening was used to find possible lead molecules from Enamine -High-Throughput Screening Center library. The resulting high binding affinity ligands were further assessed for their drug-likeness and other pharmacokinetic properties. Based on these analyses, the three ligands Z95813755_1, Z324718246_1 and Z324718246_2 were selected for further molecular dynamic simulation studies. The molecular dynamic simulation results and MM/PBSA analysis predicted that both Z95813755_1 and Z324718246_2, molecules show higher binding affinity towards MurG. For the first time we are reporting potential candidate inhibitors against MurG from <em>K. pneumoniae</em>, providing new insights in management of multi drug resistant <em>K. pneumoniae</em> infections.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"176 1","pages":"Article 104257"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterisation of a new virulent phage isolated from Hainan Island with potential against multidrug-resistant Pseudomonas aeruginosa infections 从海南岛分离出的一种新型毒性噬菌体的特征，该噬菌体具有抗耐多药型铜绿假单胞菌感染的潜力。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2025-01-01 DOI: 10.1016/j.resmic.2024.104250

Anyang Li , Chen Chen , Yanmei Li , Yanshuang Wang , Xuemiao Li , Qiao Zhu , Yue Zhang , Shen Tian , Qianfeng Xia

{"title":"Characterisation of a new virulent phage isolated from Hainan Island with potential against multidrug-resistant Pseudomonas aeruginosa infections","authors":"Anyang Li , Chen Chen , Yanmei Li , Yanshuang Wang , Xuemiao Li , Qiao Zhu , Yue Zhang , Shen Tian , Qianfeng Xia","doi":"10.1016/j.resmic.2024.104250","DOIUrl":"10.1016/j.resmic.2024.104250","url":null,"abstract":"<div><div>Multidrug-resistant (MDR) <em>Pseudomonas aeruginosa</em> is a serious life-threatening pathogen. The rise in <em>P. aeruginosa</em> resistance rates has renewed interest in phages as an alternative therapeutic approach for treating bacterial infections. In this study, we investigated the characteristics of the first <em>Pseudomonas</em> phage, vB_PaP_HN01, isolated from Hainan, the only tropical island in China. The lytic rate of this phage against <em>P. aeruginosa</em> reached 64.3 % (27/42). Under the optimal multiplicity of infection (MOI) of 0.1, more than 90 % of phage particles absorb onto the host cell within 10 min, with an eclipse period of around 15 min, and a high titer phage production (10<sup>11</sup> PFU/ml) within 90 min was demonstrated. vB_PaP_HN01 maintains a robust titer after 1 h exposure to pH values and temperatures (up to 50 °C). Genome annotation revealed that vB_PaP_HN01 did not contain drug-resistance or lysogeny-associated genes. It can effectively inhibit the formation of biofilms of MDR <em>P. aeruginosa</em> and eliminated aggressive biofilms (removal rate about 70 %). In the in vivo infection models, it was demonstrated that the survival rate and lifespan of <em>Galleria mellonella</em> larvae were increased alongside the injection of vB_PaP_HN01. These data revealed the potential of vB_PaP_HN01 against <em>P. aeruginosa</em> in clinic.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"176 1","pages":"Article 104250"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142547123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Both GacS-regulated lipopeptides and the type three secretion system contribute to Pseudomonas cichorii induced necrosis in lettuce and chicory GacS调控的脂肽和三型分泌系统都有助于cichorii假单胞菌诱导莴苣和菊苣坏死。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2025-01-01 DOI: 10.1016/j.resmic.2024.104249

Chien-Jui Huang , Ellen Pauwelyn , Marc Ongena , Peter Bleyaert , Monica Höfte

{"title":"Both GacS-regulated lipopeptides and the type three secretion system contribute to Pseudomonas cichorii induced necrosis in lettuce and chicory","authors":"Chien-Jui Huang , Ellen Pauwelyn , Marc Ongena , Peter Bleyaert , Monica Höfte","doi":"10.1016/j.resmic.2024.104249","DOIUrl":"10.1016/j.resmic.2024.104249","url":null,"abstract":"<div><div><em>Pseudomonas cichorii</em> SF1-54, the causal agent of lettuce midrib rot disease, produces lipopeptides cichofactins and cichopeptins which are important virulence factors. The GacS/GacA two-component system is well known to regulate production of lipopeptides in pseudomonads. Additionally, the functions of the type three secretion system (T3SS) in <em>P. cichorii</em>-plant interactions are not clarified. In this study, we investigated the role of the GacS-regulated lipopeptides and the T3SS in pathogenicity of <em>P. cichorii</em> SF1-54 on two host plants, chicory and lettuce, by constructing mutants in <em>hrpL</em>, which encodes the key sigma factor to control T3SS expression, and <em>gacS</em>. Compared with the wildtype, the <em>hrpL</em> mutant produced lipopeptides at a similar level but the <em>gacS</em> mutant was strongly impaired in lipopeptide production. The mutant deficient in <em>hrpL</em> did not significantly differ from the wildtype in virulence on chicory and lettuce. The <em>gacS</em> mutant exhibited significantly less symptoms on both host plants compared to the wildtype and the <em>hrpL</em> mutant. Intriguingly, the <em>gacS hrpL</em>-double mutant no longer produced lipopeptides, lost virulence and showed impaired colonization on chicory, but was still weakly virulent on lettuce. Thus, contribution of both the GacS-regulated lipopeptides and T3SS to virulence of <em>P. cichorii</em> SF1-54 is host plant dependent.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"176 1","pages":"Article 104249"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of tailocins of Pragia fontium 24613 and the tailocin loci within the family Budviciaceae Pragia fontium 24613 的尾丝素特征及芽苣苔科（Budviciaceae）内的尾丝素基因座。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2025-01-01 DOI: 10.1016/j.resmic.2024.104261

Kateřina Snopková , Eva Chaloupková , Matěj Hrala , David Šmajs

{"title":"Characterization of tailocins of Pragia fontium 24613 and the tailocin loci within the family Budviciaceae","authors":"Kateřina Snopková , Eva Chaloupková , Matěj Hrala , David Šmajs","doi":"10.1016/j.resmic.2024.104261","DOIUrl":"10.1016/j.resmic.2024.104261","url":null,"abstract":"<div><div>Tailocins are nano-scale phage tail-like protein complexes that can mediate antagonistic interactions between closely related bacterial species. While the capacity to produce R-type tailocin was found widely across Gammaproteobacteria, the production of F-type tailocins seems comparatively rare. In this study, we examined the freshwater isolate, <em>Pragia fontium</em> 24613, which can produce both R- and F-type tailocins. We investigated their inhibition spectrum, focusing on clinically relevant enterobacteria, and identified the associated tailocin gene cluster. Transmission electron microscopy confirmed that inactivation of the tape measure protein within the tailocin cluster disrupted R-tailocin production. Comparative analysis of <em>Budviciaceae</em> gene clusters showed high conservation of R-type tailocin genes, whereas F-type tailocin genes were found in only a few species, with little conservation. Our findings indicate a high prevalence of bacteriocin production among underexplored Enterobacteriales species. Detected tailocins showed potential as antimicrobials targeting clinically significant pathogens.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"176 1","pages":"Article 104261"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The roots of the Institut Pasteur's “Grand Cours” 巴斯德研究所“大课程”的根源。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2025-01-01 DOI: 10.1016/j.resmic.2024.104259

Antoine Dampierre , Sandra Legout , Emmanuel Drouin

{"title":"The roots of the Institut Pasteur's “Grand Cours”","authors":"Antoine Dampierre , Sandra Legout , Emmanuel Drouin","doi":"10.1016/j.resmic.2024.104259","DOIUrl":"10.1016/j.resmic.2024.104259","url":null,"abstract":"<div><div>The article presents an analysis of the history of the microbiology course delivered during the inaugural operational year of the Institut Pasteur in Paris. The year 1889 is examined through the lens of three hitherto unknown volumes that bring together the microbiology lectures delivered at the end of the 19th century.</div><div>The course was entirely independent from the teaching provided by the universities or faculties of medicine and rapidly gained international recognition. Indeed, the course provided the students with the theoretical knowledge of Pasteurian theories regarding the completely new discipline of microbiology and the specific techniques used to cultivate, conserve, and observe microbes. The steady increase in the number of lectures between 1889 and 1914 reflects the expansion of microbiological knowledge during this period. The contributions of researchers such as Émile Roux (1853–1933), Élie Metchnikoff (1845–1916), and Amédée Borrel (1867–1936) illuminated the collaboration and the growing diversification of expertise at the heart of the Institut Pasteur (IP).</div><div>Furthermore, this study highlights the international influence of the course, as evidenced by the participation of foreign students. It examines the history of the course as a powerful tool for disseminating knowledge of new microbiological techniques and the results of research carried out in Pasteur's laboratories. It also examines how the course served as a political instrument, asserting the authority of the Institut Pasteur in the field of microbiology in France and extending its influence worldwide.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"176 1","pages":"Article 104259"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic complexities and heterogeneity in quorum sensing signaling molecules in bacteria isolated from black band disease in a Caribbean coral 从加勒比珊瑚黑带病中分离出的细菌的代谢复杂性和法定量感应信号分子的异质性。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2025-01-01 DOI: 10.1016/j.resmic.2024.104248

Laura Ripe-Jaime , Erika Díaz , Ángel G. Franco , Catherine Keim , Daniela Burgos , Valeria Pizarro , Luis F. Cadavid , Anny Cárdenas , Catalina Arévalo-Ferro

{"title":"Metabolic complexities and heterogeneity in quorum sensing signaling molecules in bacteria isolated from black band disease in a Caribbean coral","authors":"Laura Ripe-Jaime , Erika Díaz , Ángel G. Franco , Catherine Keim , Daniela Burgos , Valeria Pizarro , Luis F. Cadavid , Anny Cárdenas , Catalina Arévalo-Ferro","doi":"10.1016/j.resmic.2024.104248","DOIUrl":"10.1016/j.resmic.2024.104248","url":null,"abstract":"<div><div>Coral diseases contribute to the worldwide loss of coral reefs, with the Black Band Disease (BBD) being a prominent example. BBD is an infectious condition with lesions with a pigmented mat composed of cyanobacteria, sulphate-reducing, sulphide-oxidizing, and heterotrophic bacteria. We compared the heterotrophic bacterial communities of healthy and BBD-affected colonies of the Caribbean coral <em>Orbicella faveolat</em><em>a</em> using culture-dependent and -independent techniques. Twenty and 23 bacterial isolates were identified from healthy and diseased tissues, respectively, which differed in their capacities to metabolize carbohydrates and citrate, either anaerobically or aerobically. They also differed in their quorum-sensing (QS) activity, as QS signaling molecules were found exclusively, and QS-inhibition was found primarily, in isolates from diseased tissues. Screening of bacterial diversity by 16SrDNA metabarcoding showed that members of the bacterial genera <em>Muricauda</em> and <em>Maritimimonas</em> were dominant in healthy tissues whereas members of the cyanobacterial genus <em>Roseofilum</em> were dominant in diseased tissues. These results suggest that bacterial dysbiosis can be linked with altered bacterial communication, likely leading to diachrony and imbalance that may participate in the progression of BBD. Investigating physiological traits and QS-based communication offers insights into the onset and progression of coral infections, paving the way for novel strategies to mitigate their impact.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"176 1","pages":"Article 104248"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of 5′-UTR nucleotide composition on translation efficiency in Escherichia coli 5'-UTR 核苷酸组成对大肠杆菌翻译效率的影响

IF 2.5 4区生物学

Research in microbiology Pub Date : 2025-01-01 DOI: 10.1016/j.resmic.2024.104260

Jinjin Li , Jiaojiao Li , Peixian Li , Jie Zhang , Qian Liu , Hao Qi

{"title":"Influence of 5′-UTR nucleotide composition on translation efficiency in Escherichia coli","authors":"Jinjin Li , Jiaojiao Li , Peixian Li , Jie Zhang , Qian Liu , Hao Qi","doi":"10.1016/j.resmic.2024.104260","DOIUrl":"10.1016/j.resmic.2024.104260","url":null,"abstract":"<div><div>Translation initiation for 5′-UTR contributes primarily to the efficient protein expression in <em>Escherichia coli</em>. Many studies have focused on constructing random 5′-UTR libraries to investigate the impact of mRNA features on protein translation efficiency. However, the study on the effect of the absence of specific types of nucleotides in the entire 5′-UTR region on translation efficiency has not yet been reported. Here, we constructed four reporter plasmid libraries encoding the sfGFP fluorescent protein, each preceded by 5′-UTRs that lack one specific nucleotide (25B, 25D, 25H, 25V). Each library was transformed into <em>E. coli</em> cells, and the fluorescence distribution among the different libraries was analyzed by flow cytometer. Additionally, we quantified the activity of 256 unique 5′-UTR sequences and analyzed the impact of the corresponding mRNA sequence features on translation efficiency. We found that the 25D library, which lacks the C nucleotide, exhibited the highest overall translation efficiency compared to the other three libraries. Moreover, the minimum free energy and 16S rRNA hybridization energy of the 5′-UTR sequence could work coordinately to influence translation efficiency. The 5′-UTR sequences lacking the C nucleotide also achieve efficient protein translation. These findings may provide several guiding principles for precisely tuning protein expression.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"176 1","pages":"Article 104260"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0