Research in microbiology最新文献_第2页

Cutibacterium acnes and its complex host interaction in prosthetic joint infection: Current insights and future directions. 痤疮表皮杆菌及其复杂的宿主相互作用在假体关节感染：目前的见解和未来的方向。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-12-17 DOI: 10.1016/j.resmic.2024.104265

Léa Thoraval, Jennifer Varin-Simon, Xavier Ohl, Frédéric Velard, Fany Reffuveille, Min Tang-Fichaux

引用次数: 0

Novel insights into Bacillus thuringiensis: Beyond its role as a bioinsecticide. 苏云金芽孢杆菌的新见解：超越其作为生物杀虫剂的作用。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-12-13 DOI: 10.1016/j.resmic.2024.104264

Gholamreza Salehi Jouzani, Reza Sharafi, Leandris Argentel-Martínez, Ofelda Peñuelas-Rubio, Ceyda Ozkan, Bengisu Incegul, Rana Goksu, Zehra Hayta, Deniz Yilki, Beyza Yazici, Vildan Hancer, Estibaliz Sansinenea, Jae-Ho Shin, A El-Shabasy, Ugur Azizoglu

引用次数: 0

Characterization and identification of Pseudomonas sp. AW4, an arsenic-resistant and plant growth-promoting bacteria isolated from the soybean (Glycine max L.) rhizosphere. 大豆（Glycine max L.）根际抗砷促进植物生长细菌Pseudomonas sp. AW4的鉴定与鉴定

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-12-06 DOI: 10.1016/j.resmic.2024.104263

Ana L Wevar Oller, Gonzalo Torres Tejerizo, Paola P Pereira, Romina Del Pilar Pramparo, Elizabeth Agostini

{"title":"Characterization and identification of Pseudomonas sp. AW4, an arsenic-resistant and plant growth-promoting bacteria isolated from the soybean (Glycine max L.) rhizosphere.","authors":"Ana L Wevar Oller, Gonzalo Torres Tejerizo, Paola P Pereira, Romina Del Pilar Pramparo, Elizabeth Agostini","doi":"10.1016/j.resmic.2024.104263","DOIUrl":"10.1016/j.resmic.2024.104263","url":null,"abstract":"<p><p>Pseudomonas sp. AW4 is a highly arsenic (As) resistant bacterium with plant growth promoting properties, originally isolated from the soybean (Glycine max L.) rhizosphere. In order to safely use this isolate in diverse bioformulations, its characterization needs to be completed and a reliable identification must be provided. In the present work, we analyzed the morpho-physiological, biochemical and genomic characteristics of Pseudomonas sp. AW4. Identification of the isolate varied according to the parameters analyzed, mainly biochemical and physiological tests or individual genes and phylogenetic analyses. In this regard, we performed massive sequencing of its genome, in order to consistently complete its characterization and identification. Pseudomonas sp. AW4 formed a monophyletic clade with P. urmiensis SWRI10, presenting 3.08 % of unique genes against this reference isolate. More than 70 % of AW4 genes were also shared with P. oryziphila strain 1257 NZ and with P. reidholzensis strain CCOS 865. The search for genes related to As resistance evidenced the presence of the operon arsHRBC. Taken together, results of the present work allow identification of this bacterium as Pseudomonas urmiensis AW4 and open up a number of opportunities to study this strain and understand the mechanisms of arsenic resistance and plant growth promotion.</p>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":" ","pages":"104263"},"PeriodicalIF":2.5,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142795021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Navigating dual-species fungal biofilms: The competitive and cooperative dynamics of Candidaalbicans. 导航双种真菌生物膜：念珠菌的竞争与合作动态。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-11-28 DOI: 10.1016/j.resmic.2024.104262

Ouassila Bekkal Brikci-Benhabib

引用次数: 0

Evolutionary trends indicate a coherent organization of sap operons 进化趋势表明，树液操作子的组织是一致的。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-11-01 DOI: 10.1016/j.resmic.2024.104228

Pratik Dasgupta, Kavya Vinil, Shankar Prasad Kanaujia

{"title":"Evolutionary trends indicate a coherent organization of sap operons","authors":"Pratik Dasgupta, Kavya Vinil, Shankar Prasad Kanaujia","doi":"10.1016/j.resmic.2024.104228","DOIUrl":"10.1016/j.resmic.2024.104228","url":null,"abstract":"<div><div><span>Human hosts possess a complex network of immune responses against microbial pathogens. The production of antimicrobial peptides (AMPs), which target the pathogen cell membranes and inhibit them from inhabiting the hosts, is one such mechanism. However, pathogens have evolved systems that encounter these host-produced AMPs. The Sap (</span><u>s</u>ensitivity to <u>a</u>ntimicrobial <u>p</u>eptides) transporter uptakes AMPs inside the microbial cell and proteolytically degrades them. The Sap transporters comprise five subunits encoded by genes in an operon. Despite its ubiquitous nature, its subunits are not found to be in tandem with many organisms. In this study, a total of 421 Sap transporters were analyzed for their operonic arrangement. Out of 421, a total of 352 operons were found to be in consensus arrangement, while the remaining 69 show a varying arrangement of genes. The analysis of the intergenic distance between the subunits of the <em>sap</em> operon suggests a signature pattern with <em>sapAB</em> (-4), <em>sapBC</em> (-14), <em>sapCD</em> (-1), and <em>sapDF</em><span> (-4 to 1). An evolutionary analysis of these operons favors the consensus arrangement of the Sap transporter systems, substantiating its prevalence in most of the Gram-negative pathogens. Overall, this study provides insight into bacterial evolution, favoring the maintenance of the genetic organization of essential pathogenicity factors.</span></div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 8","pages":"Article 104228"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthetic capabilities of Antarctic yeast Sporobolomyces roseus AL103: Temperature influence on intracellular metabolites and characterization of the exopolysaccharide 南极酵母 Sporobolomyces roseus AL103 的生物合成能力：温度对胞内代谢物的影响和外多糖的特征。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-11-01 DOI: 10.1016/j.resmic.2024.104247

Snezhana Rusinova-Videva , Manol Ognyanov , Kalina Alipieva , Stefka Nachkova , Dessislava Gerginova , Ani Petrova , Maria Marudova , Sofia Milenkova , Tsvetelina Paunova-Krasteva , Dragomir Mateev

{"title":"Biosynthetic capabilities of Antarctic yeast Sporobolomyces roseus AL103: Temperature influence on intracellular metabolites and characterization of the exopolysaccharide","authors":"Snezhana Rusinova-Videva , Manol Ognyanov , Kalina Alipieva , Stefka Nachkova , Dessislava Gerginova , Ani Petrova , Maria Marudova , Sofia Milenkova , Tsvetelina Paunova-Krasteva , Dragomir Mateev","doi":"10.1016/j.resmic.2024.104247","DOIUrl":"10.1016/j.resmic.2024.104247","url":null,"abstract":"<div><div>The purpose of the study was to investigate the biosynthetic properties of the Antarctic yeast strain <em>Sporobolomyces roseus</em> AL<sub>103</sub> in response to temperature changes, to perform intracellular metabolic profiling, and to reveal the chemical and functional characteristics of the synthesized exopolysaccharide (EPS). The results show that the yeast strain needed a shorter time to reach a stationary phase at 22 °C contrary to that of 5 °C. An NMR analysis revealed differences in metabolic profiles of amino acids, glucose, trehalose, glycerol, uridine, etc. EPS (2.9 g/L) was characterized by high–molecular-weight with total carbohydrate, uronic acids, and protein content of 66 %, 10.5 %, and 2.5 %, respectively. Mannose (74 mol%) and galactose (19 mol%) were the major constituents. The FT-IR data suggested the presence of β-(1–4)-mannan. DSC thermogram, WVTR, mechanical properties, and moisture sorption of the EPS film showed thermal stability up to 220 °C and hydrophilic behavior. The newly obtained polymer film was studied for the first time and the data showed possibilities for its successful application as a film-forming material in the preparation of packaging materials. In conclusion, the temperature influenced the metabolic profile of the Antarctic yeast producer. The biotechnological process could be directed to obtain the target intracellular or extracellular metabolites.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 8","pages":"Article 104247"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of core genome multi-locus sequence typing schemes for population structure analyses of Nocardia species 构建用于诺卡氏菌种群结构分析的核心基因组多焦点序列分型方案。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-11-01 DOI: 10.1016/j.resmic.2024.104246

Yizhak Hershko , Matan Slutzkin , Daniel Barkan , Amos Adler

{"title":"Construction of core genome multi-locus sequence typing schemes for population structure analyses of Nocardia species","authors":"Yizhak Hershko , Matan Slutzkin , Daniel Barkan , Amos Adler","doi":"10.1016/j.resmic.2024.104246","DOIUrl":"10.1016/j.resmic.2024.104246","url":null,"abstract":"<div><div>Nocardia, a member of the Actinobacteria phylum, populates diverse habitats globally, with certain species being the cause of various clinical infections in humans. There is paucity of data regarding the population structure of this genus and of established genomic-based phylogenetic methods. We examined the whole genome sequences of 193 isolates spanning five major pathogenic <em>Nocardia</em> species sourced from public databases, encompassing diverse geographic regions. Using the chewBBACA pipeline, a species-specific core genome multilocus sequence typing (cgMLST) schema was created for <em>N. cyriacigeorgica</em>, <em>N. farcinica</em>, <em>N. brasiliensis</em>, <em>N. wallacei</em>, and <em>N. abscessus</em>. Additional genomic features that were examined included virulence factor (VF) profile, total length and open-reading frame count, the core genome length and core gene count, and GC content. Our findings indicated that: (i) <em>N. brasiliensis</em> diverges significantly from the other four species, underscoring its distinct evolutionary trajectory; (ii) the population structures of all species were polyclonal, with phylogenetic clustering occurring in the minority of isolates; (iii) clonal complexes were largely restricted to specific geographical locations, rather than demonstrating a global distribution, and (iv) initial evidence suggests no direct common-source transmission amongst the studied strains. Our study establishes a comprehensive genome-based phylogenetic methodology for population structure of <em>Nocardia</em> species.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 8","pages":"Article 104246"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid lysination by MprF contributes to hemolytic pigment retention in group B Streptococcus MprF 的脂质裂解作用有助于 B 群链球菌中溶血性色素的保留。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-11-01 DOI: 10.1016/j.resmic.2024.104231

Elise Caliot , Arnaud Firon , Audrey Solgadi , Patrick Trieu-Cuot , Shaynoor Dramsi

引用次数: 0

BSC2 modulates AmB resistance via the maintenance of intracellular sodium/potassium ion homeostasis in Saccharomyces cerevisiae BSC2 通过维持酿酒酵母细胞内钠/钾离子平衡调节对 AmB 的抗性

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-11-01 DOI: 10.1016/j.resmic.2024.104245

Zhiwei Huang , Fulong Xiao , Qiao Wang , Xiaojuan Zhang , Yuhu Shen , Yunxia Deng , Ping Shi

{"title":"BSC2 modulates AmB resistance via the maintenance of intracellular sodium/potassium ion homeostasis in Saccharomyces cerevisiae","authors":"Zhiwei Huang , Fulong Xiao , Qiao Wang , Xiaojuan Zhang , Yuhu Shen , Yunxia Deng , Ping Shi","doi":"10.1016/j.resmic.2024.104245","DOIUrl":"10.1016/j.resmic.2024.104245","url":null,"abstract":"<div><div>Previous studies on <em>BSC2</em> have shown that it enhances yeast cell resistance to AmB via antioxidation and induces multidrug resistance by contributing to biofilm formation. Herein, we found that <em>BSC2</em> overexpression could reverse the sensitivity of <em>pmp3</em>Δ to AmB and help the tested strains restore the intracellular sodium/potassium balance under exposure to AmB. Meanwhile, overexpression of the chitin gene <em>CHS2</em> could simulate <em>BSC2</em> to reverse the sensitivity of <em>pmp3</em>Δ and <em>nha1</em>Δ to high salt or AmB. However, <em>BSC2</em> overexpression in <em>flo11</em>Δ failed to induce AmB resistance, form biofilms, and affect cell wall biogenesis, while <em>CHS2</em> overexpression compensated the resistance of <em>flo11</em>Δ to AmB. Additionally, <em>BSC2</em> levels were positively correlated with maintaining cell membrane integrity under exposure to AmB, CAS, or a combination of both. <em>BSC2</em> overexpression in <em>nha1</em>Δ exhibited a similar function of <em>CHS2</em>, which can compensate for the sensitivity of the mutant to high salt. Altogether, the results demonstrate for the first time that <em>BSC2</em> may promote ion equilibrium by strengthening cell walls and inhibiting membrane damage in a FLO path-dependent manner, thus enhancing the resistance of yeast cells to AmB. This study also reveals the possible mechanism of antifungal drugs CAS and AmB combined to inhibit fungi.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 8","pages":"Article 104245"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142154911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of gamma irradiation on the proteogenome of cold-acclimated Kocuria rhizophila PT10 伽马辐照对寒冷气候条件下根瘤梭菌 PT10 蛋白质基因组的影响

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-11-01 DOI: 10.1016/j.resmic.2024.104230

Sihem Guesmi , Kais Ghedira , Petar Pujic , Afef Najjari , Guylaine Miotello , Ameur Cherif , Issay Narumi , Jean Armengaud , Philippe Normand , Haïtham Sghaier

{"title":"Effect of gamma irradiation on the proteogenome of cold-acclimated Kocuria rhizophila PT10","authors":"Sihem Guesmi , Kais Ghedira , Petar Pujic , Afef Najjari , Guylaine Miotello , Ameur Cherif , Issay Narumi , Jean Armengaud , Philippe Normand , Haïtham Sghaier","doi":"10.1016/j.resmic.2024.104230","DOIUrl":"10.1016/j.resmic.2024.104230","url":null,"abstract":"<div><div>The effects of ionizing radiation (IR) on the protein dynamics of cold-stressed cells of a radioresistant actinobacterium, <em>Kocuria rhizophila</em> PT10, isolated from the rhizosphere of the desert plant <em>Panicum turgidum</em> were investigated using a shotgun methodology based on nanoflow liquid chromatography coupled to tandem mass spectrometry. Overall, 1487 proteins were certified, and their abundances were compared between the irradiated condition and control. IR of cold-acclimated PT10 triggered the over-abundance of proteins involved in (1) a strong transcriptional regulation, (2) amidation of peptidoglycan and preservation of cell envelope integrity, (3) detoxification of reactive electrophiles and regulation of the redox status of proteins, (4) base excision repair and prevention of mutagenesis and (5) the tricarboxylic acid (TCA) cycle and production of fatty acids. Also, one of the more significant findings to emerge from this study is the SOS response of stressed PT10. Moreover, a comparison of top hits radio-modulated proteins of cold-acclimated PT10 with proteomics data from gamma-irradiated <em>Deinococcus deserti</em> showed that stressed PT10 has a specific response characterised by a high over-abundance of NemA, GatD, and UdgB.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 8","pages":"Article 104230"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141875819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0