{"title":"Molecular insights into PGPR fluorescent Pseudomonads complex mediated intercellular and interkingdom signal transduction mechanisms in promoting plant's immunity","authors":"","doi":"10.1016/j.resmic.2024.104218","DOIUrl":"10.1016/j.resmic.2024.104218","url":null,"abstract":"<div><p><span><span>The growth-promoting and immune modulatory properties of different strains of plant growth promoting rhizobacteria<span> (PGPR) fluorescent Pseudomonads complex (PFPC) can be explored to combat food security challenges. These PFPC prime plants through induced systemic resistance, fortify plants to overcome future pathogen-mediated vulnerability by eliciting robust systemic acquired resistance through regulation by nonexpressor of pathogenesis-related genes 1. Moreover, outer </span></span>membrane vesicles released from </span><span><span>Pseudomonas fluorescens</span></span><span> also elicit a broad spectrum of immune responses, presenting a rapid viable alternative to whole cells. Thus, PFPC can help the host to maintain an equilibrium between growth and immunity, ultimately leads to increased crop yield.</span></p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 7","pages":"Article 104218"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141327739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Metagenomics approaches in unveiling the dynamics of Plant Growth-Promoting Microorganisms (PGPM) vis-à-vis Phytophthora sp. suppression in various crop ecological systems","authors":"","doi":"10.1016/j.resmic.2024.104217","DOIUrl":"10.1016/j.resmic.2024.104217","url":null,"abstract":"<div><p><span><span>Phytophthora</span></span><span> species are destructive pathogens causing yield losses in different ecological systems, such as potato, black pepper, pepper, avocado, citrus, and tobacco. The diversity of plant growth-promoting microorganisms (PGPM) plays a crucial role in disease suppression. Knowledge of metagenomics<span> approaches is essential for assessing the dynamics of PGPM and </span></span><em>Phytophthora</em><span> species across various ecosystems, facilitating effective management strategies for better crop protection. This review discusses the dynamic interplay between PGPM and </span><em>Phytophthora</em><span> sp. using metagenomics approaches that sheds light on the potential of PGPM strains tailored to specific crop ecosystems to bolster pathogen suppressiveness.</span></p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 7","pages":"Article 104217"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141301512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Listeria monocytogenes in beef: a hidden risk","authors":"","doi":"10.1016/j.resmic.2024.104215","DOIUrl":"10.1016/j.resmic.2024.104215","url":null,"abstract":"<div><p><span><span>Listeria monocytogenes</span></span> in beef receives less attention compared to other pathogens such as <em>Salmonella</em> and <span><em>Escherichia coli</em></span>. To address this gap, we conducted a literature review focusing on the presence of <em>L. monocytogenes</em><span> in beef. This review encompasses the pathogenic mechanisms, routes of contamination, prevalence rates, and the laws and regulations employed in various countries. Our findings reveal a prevalence of </span><em>L. monocytogenes</em><span><span> in beef and beef products ranging from 2.5% to 59.4%. Notably, serotype 4b was most frequently isolated in cases of beef contamination during food processing, with the skinning and </span>evisceration stages identified as critical points of contamination.</span></p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 7","pages":"Article 104215"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141238113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Endopeptidase activities of Clostridium botulinum toxins in the development of this bacterium","authors":"","doi":"10.1016/j.resmic.2024.104216","DOIUrl":"10.1016/j.resmic.2024.104216","url":null,"abstract":"<div><p><span>By-products like CO₂ and organic acids, produced during </span><span><span>Clostridium botulinum</span></span> growth, appear to inhibit its development and reduce ATP production. A decrease in ATP production creates an imbalance in the ATP/GTP ratio. GTP activates CodY, which regulates <span><em>BoNT</em></span><span><span><span><span> expression. This toxin is released into the extracellular medium. Its light chains act as a specific endopeptidase, targeting </span>SNARE proteins. The specific </span>amino acids released enter the cells and are metabolized by the Stickland reaction, resulting in the synthesis of ATP. This ATP might then be used by </span>histidine<span> kinases to activate Spo0A, the main regulator initiating sporulation, through phosphorylation.</span></span></p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 7","pages":"Article 104216"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141427445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"d-aspartate, an amino-acid important for human health, supports anaerobic respiration in several Campylobacter species","authors":"","doi":"10.1016/j.resmic.2024.104219","DOIUrl":"10.1016/j.resmic.2024.104219","url":null,"abstract":"<div><p>Despite being classified as microaerophilic microorganisms, most <span><span>Campylobacter</span></span> species can grow anaerobically, using formate or molecular hydrogen (H<sub>2</sub>) as electron donors, and various nitrogenous and sulfurous compounds as electron acceptors. Herein, we showed that both <span>l</span>-asparagine (<span>l</span>-Asn) and <span>l</span>-aspartic acid (<span>l</span>-Asp) bolster H<sub>2</sub><span>-driven anaerobic growth in several </span><span><em>Campylobacter</em></span> species, whereas the <span>d-</span><span><span>enantiomer form of both </span>asparagine (</span><span>d</span><span>-Asn) and aspartic acid (</span><span>d</span>-Asp) only increased anaerobic growth in <span><span>Campylobacter concisus</span></span> strain 13826 and <span><em>Campylobacter ureolyticus</em></span> strain NCTC10941. A gene annotated as <em>racD</em> encoding for a putative <span>d</span>/<span>l</span><span>-Asp racemase was identified in the genome of both strains. Disruption of </span><em>racD</em> in <em>Cc</em>13826 resulted in the inability of the mutant strain to use either <span>d-</span>enantiomer during anaerobic growth. Hence, our results suggest that the <em>racD</em> gene is required for campylobacters to use either <span>d</span>-Asp or <span>d</span>-Asn. The use of <span>d</span>-Asp by various human opportunistic bacterial pathogens, including <em>C. concisus</em>, <em>C. ureolyticus</em>, and also possibly select strains of <em>Campylobacter gracilis</em>, <span><span>Campylobacter rectus</span></span> and <em>Campylobacter showae</em>, is significant, because <span>d</span><span>-Asp is an important signal molecule for both human nervous and neuroendocrine systems. To our knowledge, this is the first report of pathogens scavenging a </span><span>d-</span><span>amino acid essential for human health.</span></p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 7","pages":"Article 104219"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141470480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biofilms in soils: The evidence about sessile versus planktonic microorganisms needs revisiting","authors":"","doi":"10.1016/j.resmic.2024.104204","DOIUrl":"10.1016/j.resmic.2024.104204","url":null,"abstract":"","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 7","pages":"Article 104204"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140896930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nasreen Amin , Rajeshwar P. Sinha , Vinod K. Kannaujiya
{"title":"Effects of ultraviolet and photosynthetically active radiation on morphogenesis, antioxidants and photoprotective defense mechanism in a hot-spring cyanobacterium Nostoc sp. strain VKB02","authors":"Nasreen Amin , Rajeshwar P. Sinha , Vinod K. Kannaujiya","doi":"10.1016/j.resmic.2024.104180","DOIUrl":"10.1016/j.resmic.2024.104180","url":null,"abstract":"<div><p><span><span>The continuous increase in global temperature and ultraviolet radiation (UVR) causes profound impacts on the growth and physiology of photosynthetic microorganisms. The hot-spring </span>cyanobacteria have a wide range of mitigation mechanisms to cope up against current unsustainable environmental conditions. In the present investigation, we have explored the indispensable mitigation strategies of an isolated hot-spring cyanobacterium </span><span><em>Nostoc</em></span><span> sp. strain VKB02 under simulated ultraviolet (UV-A, UV-B) and photosynthetically active radiation (PAR). The adaptive morphological changes were more significantly observed under PAB (PAR, UV-A, and UV-B) exposure as compared to P and PA (PAR and UV-A) irradiations. PAB exposure also exhibited a marked decline in pigment composition and photosynthetic efficiency by multi-fold increment of free radicals. To counteract the oxidative stress, enzymatic and non-enzymatic antioxidants defense were significantly enhanced many folds under PAB exposure as compared to the control. In addition, the cyanobacterium has also produced shinorine as a strong free radicals scavenger and excellent UV absorber for effective photoprotection against UV radiation. Therefore, the hot-spring cyanobacterium </span><em>Nostoc</em> sp. strain VKB02 has unique defense strategies for survival under prolonged lethal UVR conditions. This study will help in the understanding of environment-induced defense strategies and production of highly value-added green photo-protectants for commercial applications.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 5","pages":"Article 104180"},"PeriodicalIF":2.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139417962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Feng Peng , Yu Zou , Xiuxia Liu , Yankun Yang , Jing Chen , Jianqi Nie , Danni Huang , Zhonghu Bai
{"title":"The murein endopeptidase MepA regulated by MtrAB and MprAB participate in cell wall homeostasis","authors":"Feng Peng , Yu Zou , Xiuxia Liu , Yankun Yang , Jing Chen , Jianqi Nie , Danni Huang , Zhonghu Bai","doi":"10.1016/j.resmic.2024.104188","DOIUrl":"10.1016/j.resmic.2024.104188","url":null,"abstract":"<div><p>The complete genome of <span><em>Corynebacterium glutamicum</em></span><span> contain a gene encoding murein endopeptidase MepA which maintain cell wall homeostasis<span> by regulating peptidoglycan biosynthesis. In this study, we investigate the physiological function, localization and regulator of MepA. The result shows that </span></span><em>mepA</em> overexpression lead to peptidoglycan degradation and the defects in cell division. MepA-EGFP was shown to localizes exclusively at the cell cell septum. In addition, <em>mepA</em><span> overexpression increased cell permeability and reduced the resistance of cells to isoniazid, an antibiotic used to treat </span><span><em>Mycobacterium tuberculosis</em></span> infection. Furthermore, transcription analysis showed that <em>mepA</em><span> affected cell division and membrane transport pathways, and was coordinately regulated by the two-component systems MtrAB and MprAB(CgtS/R2).</span></p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 5","pages":"Article 104188"},"PeriodicalIF":2.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139576630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rachel Javorova , Beatrica Sevcikova , Bronislava Rezuchova, Renata Novakova, Filip Opaterny, Dominika Csolleiova, Lubomira Feckova, Jan Kormanec
{"title":"Multiple SigB homologues govern the transcription of the ssgBp promoter in the sporulation–specific ssgB gene in Streptomyces coelicolor A3(2)","authors":"Rachel Javorova , Beatrica Sevcikova , Bronislava Rezuchova, Renata Novakova, Filip Opaterny, Dominika Csolleiova, Lubomira Feckova, Jan Kormanec","doi":"10.1016/j.resmic.2024.104201","DOIUrl":"10.1016/j.resmic.2024.104201","url":null,"abstract":"<div><p>Unlike <em>Bacillus subtilis</em>, <em>Streptomyces coelicolor</em> contains nine SigB homologues of the stress-response sigma factor SigB. By using a two-plasmid system, we previously identified promoters recognized by these sigma factors. Almost all promoters were recognized by several SigB homologues. However, no specific sequences of these promoters were found. One of these promoters, <em>ssgBp</em>, was selected to examine this cross-recognition in the native host. It controls the expression of the sporulation-specific gene <em>ssgB</em>. Using a luciferase reporter, the activity of this promoter in <em>S. coelicolor</em> and nine mutant strains lacking individual <em>sigB</em> homologous genes showed that <em>sgBp</em> is dependent on three sigma factors, SigH, SigN, and SigI. To determine which nucleotides in the-10 region are responsible for the selection of a specific SigB homologue, promoters mutated at the last three nucleotide positions were tested in the two-plasmid system. Some mutant promoters were specifically recognized by a distinct set of SigB homologues. Analysis of these mutant promoters in the native host showed the role of these nucleotides. A conserved nucleotide A at position 5 was essential for promoter activity, and two variable nucleotides at positions 4 and 6 were responsible for the partial selectivity of promoter recognition by SigB homologues.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 5","pages":"Article 104201"},"PeriodicalIF":2.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ofélia Godinho , Damien P. Devos , Sandra Quinteira , Olga M. Lage
{"title":"The influence of the phylum Planctomycetota in the environmental resistome","authors":"Ofélia Godinho , Damien P. Devos , Sandra Quinteira , Olga M. Lage","doi":"10.1016/j.resmic.2024.104196","DOIUrl":"10.1016/j.resmic.2024.104196","url":null,"abstract":"<div><p>Antimicrobial resistance is one of the leading causes of death worldwide and research on this topic has been on the spotlight for a long time. More recently and in agreement with the One Health Approach, the focus has moved towards the environmental resistome. Members of the phylum <em>Planctomycetota</em> are ubiquitously present in the environment including in hotspots for antimicrobial resistance selection and dissemination. Furthermore, phenotypic broad-range resistance has been observed in diverse members of this phylum. Here we review the evidence available on antimicrobial resistance in the underexploited <em>Planctomycetota</em> and highlight key aspects for future studies.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 5","pages":"Article 104196"},"PeriodicalIF":2.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0923250824000226/pdfft?md5=44d481a10d48dfcd6c3b140cfe329afb&pid=1-s2.0-S0923250824000226-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140102356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}