Research in microbiology最新文献_第3页

Construction of core genome multi-locus sequence typing schemes for population structure analyses of Nocardia species 构建用于诺卡氏菌种群结构分析的核心基因组多焦点序列分型方案。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-11-01 DOI: 10.1016/j.resmic.2024.104246

Yizhak Hershko , Matan Slutzkin , Daniel Barkan , Amos Adler

{"title":"Construction of core genome multi-locus sequence typing schemes for population structure analyses of Nocardia species","authors":"Yizhak Hershko , Matan Slutzkin , Daniel Barkan , Amos Adler","doi":"10.1016/j.resmic.2024.104246","DOIUrl":"10.1016/j.resmic.2024.104246","url":null,"abstract":"<div><div>Nocardia, a member of the Actinobacteria phylum, populates diverse habitats globally, with certain species being the cause of various clinical infections in humans. There is paucity of data regarding the population structure of this genus and of established genomic-based phylogenetic methods. We examined the whole genome sequences of 193 isolates spanning five major pathogenic Nocardia species sourced from public databases, encompassing diverse geographic regions. Using the chewBBACA pipeline, a species-specific core genome multilocus sequence typing (cgMLST) schema was created for N. cyriacigeorgica, N. farcinica, N. brasiliensis, N. wallacei, and N. abscessus. Additional genomic features that were examined included virulence factor (VF) profile, total length and open-reading frame count, the core genome length and core gene count, and GC content. Our findings indicated that: (i) N. brasiliensis diverges significantly from the other four species, underscoring its distinct evolutionary trajectory; (ii) the population structures of all species were polyclonal, with phylogenetic clustering occurring in the minority of isolates; (iii) clonal complexes were largely restricted to specific geographical locations, rather than demonstrating a global distribution, and (iv) initial evidence suggests no direct common-source transmission amongst the studied strains. Our study establishes a comprehensive genome-based phylogenetic methodology for population structure of Nocardia species.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 8","pages":"Article 104246"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid lysination by MprF contributes to hemolytic pigment retention in group B Streptococcus MprF 的脂质裂解作用有助于 B 群链球菌中溶血性色素的保留。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-11-01 DOI: 10.1016/j.resmic.2024.104231

Elise Caliot , Arnaud Firon , Audrey Solgadi , Patrick Trieu-Cuot , Shaynoor Dramsi

引用次数: 0

BSC2 modulates AmB resistance via the maintenance of intracellular sodium/potassium ion homeostasis in Saccharomyces cerevisiae BSC2 通过维持酿酒酵母细胞内钠/钾离子平衡调节对 AmB 的抗性

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-11-01 DOI: 10.1016/j.resmic.2024.104245

Zhiwei Huang , Fulong Xiao , Qiao Wang , Xiaojuan Zhang , Yuhu Shen , Yunxia Deng , Ping Shi

{"title":"BSC2 modulates AmB resistance via the maintenance of intracellular sodium/potassium ion homeostasis in Saccharomyces cerevisiae","authors":"Zhiwei Huang , Fulong Xiao , Qiao Wang , Xiaojuan Zhang , Yuhu Shen , Yunxia Deng , Ping Shi","doi":"10.1016/j.resmic.2024.104245","DOIUrl":"10.1016/j.resmic.2024.104245","url":null,"abstract":"<div><div>Previous studies on BSC2 have shown that it enhances yeast cell resistance to AmB via antioxidation and induces multidrug resistance by contributing to biofilm formation. Herein, we found that BSC2 overexpression could reverse the sensitivity of pmp3Δ to AmB and help the tested strains restore the intracellular sodium/potassium balance under exposure to AmB. Meanwhile, overexpression of the chitin gene CHS2 could simulate BSC2 to reverse the sensitivity of pmp3Δ and nha1Δ to high salt or AmB. However, BSC2 overexpression in flo11Δ failed to induce AmB resistance, form biofilms, and affect cell wall biogenesis, while CHS2 overexpression compensated the resistance of flo11Δ to AmB. Additionally, BSC2 levels were positively correlated with maintaining cell membrane integrity under exposure to AmB, CAS, or a combination of both. BSC2 overexpression in nha1Δ exhibited a similar function of CHS2, which can compensate for the sensitivity of the mutant to high salt. Altogether, the results demonstrate for the first time that BSC2 may promote ion equilibrium by strengthening cell walls and inhibiting membrane damage in a FLO path-dependent manner, thus enhancing the resistance of yeast cells to AmB. This study also reveals the possible mechanism of antifungal drugs CAS and AmB combined to inhibit fungi.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 8","pages":"Article 104245"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142154911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of gamma irradiation on the proteogenome of cold-acclimated Kocuria rhizophila PT10 伽马辐照对寒冷气候条件下根瘤梭菌 PT10 蛋白质基因组的影响

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-11-01 DOI: 10.1016/j.resmic.2024.104230

Sihem Guesmi , Kais Ghedira , Petar Pujic , Afef Najjari , Guylaine Miotello , Ameur Cherif , Issay Narumi , Jean Armengaud , Philippe Normand , Haïtham Sghaier

{"title":"Effect of gamma irradiation on the proteogenome of cold-acclimated Kocuria rhizophila PT10","authors":"Sihem Guesmi , Kais Ghedira , Petar Pujic , Afef Najjari , Guylaine Miotello , Ameur Cherif , Issay Narumi , Jean Armengaud , Philippe Normand , Haïtham Sghaier","doi":"10.1016/j.resmic.2024.104230","DOIUrl":"10.1016/j.resmic.2024.104230","url":null,"abstract":"<div><div>The effects of ionizing radiation (IR) on the protein dynamics of cold-stressed cells of a radioresistant actinobacterium, Kocuria rhizophila PT10, isolated from the rhizosphere of the desert plant Panicum turgidum were investigated using a shotgun methodology based on nanoflow liquid chromatography coupled to tandem mass spectrometry. Overall, 1487 proteins were certified, and their abundances were compared between the irradiated condition and control. IR of cold-acclimated PT10 triggered the over-abundance of proteins involved in (1) a strong transcriptional regulation, (2) amidation of peptidoglycan and preservation of cell envelope integrity, (3) detoxification of reactive electrophiles and regulation of the redox status of proteins, (4) base excision repair and prevention of mutagenesis and (5) the tricarboxylic acid (TCA) cycle and production of fatty acids. Also, one of the more significant findings to emerge from this study is the SOS response of stressed PT10. Moreover, a comparison of top hits radio-modulated proteins of cold-acclimated PT10 with proteomics data from gamma-irradiated Deinococcus deserti showed that stressed PT10 has a specific response characterised by a high over-abundance of NemA, GatD, and UdgB.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 8","pages":"Article 104230"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141875819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endophyte mediated biocontrol mechanisms of phytopathogens in agriculture 内生菌介导的农业植物病原体生物控制机制。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-11-01 DOI: 10.1016/j.resmic.2024.104229

Muhammad Fazle Rabbee , Md. Sarafat Ali , Md. Nurul Islam , Mohammed M. Rahman , Md. Mohidul Hasan , Kwang-Hyun Baek

{"title":"Endophyte mediated biocontrol mechanisms of phytopathogens in agriculture","authors":"Muhammad Fazle Rabbee , Md. Sarafat Ali , Md. Nurul Islam , Mohammed M. Rahman , Md. Mohidul Hasan , Kwang-Hyun Baek","doi":"10.1016/j.resmic.2024.104229","DOIUrl":"10.1016/j.resmic.2024.104229","url":null,"abstract":"<div><div>The global human population is growing and demand for food is increasing. Global agriculture faces numerous challenges, including excessive application of synthetic pesticides, emergence of herbicide-and pesticide-resistant pathogenic microbes, and more frequent natural disasters associated with global warming. Searches for valuable endophytes have increased, with the aim of making agriculture more sustainable and environmentally friendly. Endophytic microbes are known to have a variety of beneficial effects on plants. They can effectively transfer nutrients from the soil into plants, promote plant growth and development, increase disease resistance, increase stress tolerance, prevent herbivore feeding, reduce the virulence of pathogens, and inhibit the growth of rival plant species. Endophytic microbes can considerably minimize the need for agrochemicals, such as fertilizers, fungicides, bactericides, insecticides, and herbicides in the cultivation of crop plants. This review summarizes current knowledge on the roles of endophytes focusing on their mechanisms of disease control against phytopathogens through the secretion of antimicrobial substances and volatile organic compounds, and the induction of systemic resistance in plants. Additionally, the beneficial roles of these endophytes and their metabolites in the control of postharvest diseases in plants have been summarized.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 8","pages":"Article 104229"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141591208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Roles of Pseudomonas aeruginosa siderophores in interaction with prokaryotic and eukaryotic organisms 铜绿假单胞菌苷元在与原核生物和真核生物相互作用中的作用。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-09-01 DOI: 10.1016/j.resmic.2024.104211

{"title":"Roles of Pseudomonas aeruginosa siderophores in interaction with prokaryotic and eukaryotic organisms","authors":"","doi":"10.1016/j.resmic.2024.104211","DOIUrl":"10.1016/j.resmic.2024.104211","url":null,"abstract":"<div>Pseudomonas aeruginosa is an opportunistic pathogen that produces two types of siderophores, pyoverdine and pyochelin, that play pivotal roles in iron scavenging from the environment and host cells. P. aeruginosa siderophores can serve as virulence factors and perform various functions. Several bacterial and fungal species are likely to interact with P. aeruginosa due to its ubiquity in soil and water as well as its potential to cause infections in plants, animals, and humans. Siderophores produced by P. aeruginosa play critical roles in iron scavenging for prokaryotic species (bacteria) and eukaryotic hosts (fungi, animals, insects, invertebrates, and plants) as well. This review provides a comprehensive discussion of the role of P. aeruginosa siderophores in interaction with prokaryotes and eukaryotes as well as their underlying mechanisms of action. The evolutionary relationship between P. aeruginosa siderophore recognition receptors, such as FpvA, FpvB, and FptA, and those of other bacterial species has also been investigated.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 7","pages":"Article 104211"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140909218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of β-ionone on bacterial cells: the use of specific lux-biosensors β-酮对细菌细胞的影响：特定勒克斯生物传感器的使用。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-09-01 DOI: 10.1016/j.resmic.2024.104214

{"title":"The effect of β-ionone on bacterial cells: the use of specific lux-biosensors","authors":"","doi":"10.1016/j.resmic.2024.104214","DOIUrl":"10.1016/j.resmic.2024.104214","url":null,"abstract":"<div>The diversity of the biological activity of volatile organic compounds (VOCs), including unsaturated ketone β-ionone, promising pharmacological, biotechnological, and agricultural agent, has aroused considerable interest. However, the functional role and mechanisms of action of VOCs remain insufficiently studied. In this work, the response of bacterial cells to the action of β-ionone was studied using specific bioluminescent lux-biosensors containing stress-sensitive promoters. We determined that in Escherichia coli cells, β-ionone induces oxidative stress (PkatG and Pdps promoters) through a specific response mediated by the OxyR/OxyS regulon, but not SoxR/SoxS (PsoxS promoter). It has been shown that β-ionone at high concentrations (50 μM and above) causes a weak induction of the expression from the PibpA promoter and slightly induces the PcolD promoter in the E. coli biosensors; the observed effect is enhanced in the ΔoxyR mutants. This indicates the presence of some damage to proteins and DNA. β-Ionone was found to inhibit the bichaperone-dependent DnaKJE-ClpB refolding of heat-inactivated bacterial luciferase in E. coli wild-type and ΔibpB mutant strains. In the cells of the Gram-positive bacterium Bacillus subtilis 168 pNK-MrgA β-ionone does not cause oxidative stress. Thus, in this work, the specificity of bacterial cell stress responses to the action of β-ionone was shown.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 7","pages":"Article 104214"},"PeriodicalIF":2.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140916647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular insights into PGPR fluorescent Pseudomonads complex mediated intercellular and interkingdom signal transduction mechanisms in promoting plant's immunity PGPR荧光假单胞菌复合体介导的细胞间和王国间信号转导机制在促进植物免疫方面的分子见解。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-09-01 DOI: 10.1016/j.resmic.2024.104218

引用次数: 0

Metagenomics approaches in unveiling the dynamics of Plant Growth-Promoting Microorganisms (PGPM) vis-à-vis Phytophthora sp. suppression in various crop ecological systems 元基因组学方法揭示了各种作物生态系统中植物生长促进微生物 (PGPM) 与抑制疫霉菌 (Phytophthora sp.) 的动态关系。

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-09-01 DOI: 10.1016/j.resmic.2024.104217

引用次数: 0

Listeria monocytogenes in beef: a hidden risk 牛肉中的李斯特菌：隐藏的风险

IF 2.5 4区生物学

Research in microbiology Pub Date : 2024-09-01 DOI: 10.1016/j.resmic.2024.104215

引用次数: 0