Effect of gamma irradiation on the proteogenome of cold-acclimated Kocuria rhizophila PT10

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Research in microbiology Pub Date : 2024-11-01 DOI:10.1016/j.resmic.2024.104230

Sihem Guesmi , Kais Ghedira , Petar Pujic , Afef Najjari , Guylaine Miotello , Ameur Cherif , Issay Narumi , Jean Armengaud , Philippe Normand , Haïtham Sghaier

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Abstract

The effects of ionizing radiation (IR) on the protein dynamics of cold-stressed cells of a radioresistant actinobacterium, Kocuria rhizophila PT10, isolated from the rhizosphere of the desert plant Panicum turgidum were investigated using a shotgun methodology based on nanoflow liquid chromatography coupled to tandem mass spectrometry. Overall, 1487 proteins were certified, and their abundances were compared between the irradiated condition and control. IR of cold-acclimated PT10 triggered the over-abundance of proteins involved in (1) a strong transcriptional regulation, (2) amidation of peptidoglycan and preservation of cell envelope integrity, (3) detoxification of reactive electrophiles and regulation of the redox status of proteins, (4) base excision repair and prevention of mutagenesis and (5) the tricarboxylic acid (TCA) cycle and production of fatty acids. Also, one of the more significant findings to emerge from this study is the SOS response of stressed PT10. Moreover, a comparison of top hits radio-modulated proteins of cold-acclimated PT10 with proteomics data from gamma-irradiated Deinococcus deserti showed that stressed PT10 has a specific response characterised by a high over-abundance of NemA, GatD, and UdgB.

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伽马辐照对寒冷气候条件下根瘤梭菌 PT10 蛋白质基因组的影响

本文采用基于纳米液相色谱-串联质谱（nano LC-MS/MS）的霰弹枪方法，研究了电离辐射（IR）对抗辐射放线菌 Kocuria rhizophila PT10 冷应激细胞蛋白质动态的影响。共认证了 1,487 种蛋白质，并比较了辐照条件和对照条件下的蛋白质丰度。对冷螯合 PT10 进行红外辐照会导致参与以下活动的蛋白质过度富集：(1) 强有力的转录调控；(2) 肽聚糖的酰胺化和细胞包膜完整性的保护；(3) 活性亲电子物的解毒和蛋白质氧化还原状态的调控；(4) 碱基切除修复和突变的预防；(5) 三羧酸（TCA）循环和脂肪酸的产生。此外，这项研究的一个重要发现是受压 PT10 的 SOS 响应。此外，将冷气候条件下 PT10 的最高命中率放射性调制蛋白与伽马辐照沙漠化德氏球菌的蛋白质组学数据进行比较后发现，受胁迫的 PT10 有一种特殊的反应，其特征是 NemA、GatD 和 UdgB 这两种转录调节因子的高过丰度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research in microbiology 生物-微生物学

CiteScore

4.10

自引率

3.80%

发文量

审稿时长

16 days

期刊介绍： Research in Microbiology is the direct descendant of the original Pasteur periodical entitled Annales de l''Institut Pasteur, created in 1887 by Emile Duclaux under the patronage of Louis Pasteur. The Editorial Committee included Chamberland, Grancher, Nocard, Roux and Straus, and the first issue began with Louis Pasteur''s "Lettre sur la Rage" which clearly defines the spirit of the journal:"You have informed me, my dear Duclaux, that you intend to start a monthly collection of articles entitled "Annales de l''Institut Pasteur". You will be rendering a service that will be appreciated by the ever increasing number of young scientists who are attracted to microbiological studies. In your Annales, our laboratory research will of course occupy a central position, but the work from outside groups that you intend to publish will be a source of competitive stimulation for all of us."That first volume included 53 articles as well as critical reviews and book reviews. From that time on, the Annales appeared regularly every month, without interruption, even during the two world wars. Although the journal has undergone many changes over the past 100 years (in the title, the format, the language) reflecting the evolution in scientific publishing, it has consistently maintained the Pasteur tradition by publishing original reports on all aspects of microbiology.