GefC, a protein possessing a GGDEF domain, regulates biofilm formation, swimming motility, and pathogenesis in Vibrio parahaemolyticus.

IF 3.4 4区生物学 Q3 MICROBIOLOGY

Research in microbiology Pub Date : 2025-09-11 DOI:10.1016/j.resmic.2025.104338

Yining Zhou, Yiquan Zhang, Bin Ni, Jingyang Chang, Renfei Lu, Qing Xu

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引用次数: 0

Abstract

Cyclic di-guanosine monophosphate (c-di-GMP), a bacterial second messenger, regulates diverse key processes including virulence, biofilm formation, and motility. Its synthesis involves diguanylate cyclases (DGCs) with GGDEF domains, while degradation is mediated by phosphodiesterases (PDEs) containing EAL or HD-GYP domains. This study examines the role of GefC (VP0486), a GGDEF domain protein in Vibrio parahaemolyticus, in c-di-GMP signaling, biofilm dynamics, motility, and virulence. Deletion of gefC markedly reduced cellular c-di-GMP, impaired biofilm formation, and reduced matrix components (exopolysaccharides, extracellular proteins, and extracellular DNA). Conversely, the ΔgefC mutant exhibited enhanced swimming motility and increased cytotoxicity and hemolytic activity, while zebrafish infection assays revealed attenuated lethality. Transcriptional analysis showed GefC differentially regulates EPS-related gene (cpsA), polar flagellar gene (flgM), type III secretion system 1 (vopN and vp1687), type VI secretion system 2 (hcp2, vpa1043, and vpa1044), and the TDH-encoding tdh2. Genetic evidence confirmed vp0485 and gefC form an operon. These findings establish GefC as a critical regulator of c-di-GMP-dependent biofilm development, motility, and virulence in V. parahaemolyticus, highlighting its multifaceted role in pathogenicity.

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GefC是一种具有GGDEF结构域的蛋白，调节副溶血性弧菌的生物膜形成、游动运动和发病机制。

环二鸟苷单磷酸（c-di-GMP）是细菌的第二信使，调节多种关键过程，包括毒力、生物膜形成和运动。其合成涉及具有GGDEF结构域的二胍酸环化酶（DGCs），而降解则由含有EAL或HD-GYP结构域的磷酸二酯酶（PDEs）介导。本研究探讨了副溶血性弧菌GGDEF结构域蛋白GefC （VP0486）在c-di-GMP信号、生物膜动力学、运动和毒力中的作用。gefC的缺失显著降低了细胞c-二gmp，破坏了生物膜的形成，减少了基质成分（胞外多糖、细胞外蛋白和细胞外DNA）。相反，ΔgefC突变体表现出增强的游泳运动性和增加的细胞毒性和溶血活性，而斑马鱼感染试验显示毒性减弱。转录分析显示，GefC差异调控eps相关基因（cpsA）、极性鞭毛基因（flgM）、III型分泌系统1 （vopN和vp1687）、VI型分泌系统2 （hcp2、vpa1043和vpa1044）以及tdh编码的tdh2。遗传证据证实vp0485和gefC形成一个操纵子。这些发现表明GefC是副溶血性弧菌c-di- gmp依赖性生物膜发育、运动和毒力的关键调节因子，突出了其在致病性中的多方面作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research in microbiology 生物-微生物学

CiteScore

4.10

自引率

3.80%

发文量

审稿时长

16 days

期刊介绍： Research in Microbiology is the direct descendant of the original Pasteur periodical entitled Annales de l''Institut Pasteur, created in 1887 by Emile Duclaux under the patronage of Louis Pasteur. The Editorial Committee included Chamberland, Grancher, Nocard, Roux and Straus, and the first issue began with Louis Pasteur''s "Lettre sur la Rage" which clearly defines the spirit of the journal:"You have informed me, my dear Duclaux, that you intend to start a monthly collection of articles entitled "Annales de l''Institut Pasteur". You will be rendering a service that will be appreciated by the ever increasing number of young scientists who are attracted to microbiological studies. In your Annales, our laboratory research will of course occupy a central position, but the work from outside groups that you intend to publish will be a source of competitive stimulation for all of us."That first volume included 53 articles as well as critical reviews and book reviews. From that time on, the Annales appeared regularly every month, without interruption, even during the two world wars. Although the journal has undergone many changes over the past 100 years (in the title, the format, the language) reflecting the evolution in scientific publishing, it has consistently maintained the Pasteur tradition by publishing original reports on all aspects of microbiology.