Research in microbiology最新文献

{"title":"IS257-mediated amplification of tet(L) variant as a novel mechanism of enhanced tigecycline resistance in Staphylococcus cohnii","authors":"Hong Yao ,&nbsp;Hongjie Xing ,&nbsp;Nannan Wang ,&nbsp;Likuan Zhang ,&nbsp;Stefan Schwarz ,&nbsp;Chenglong Li ,&nbsp;Chang Cai ,&nbsp;Chunyan Xu ,&nbsp;Xiang-Dang Du","doi":"10.1016/j.resmic.2023.104114","DOIUrl":"10.1016/j.resmic.2023.104114","url":null,"abstract":"<div><p>The mechanism of enhanced tigecycline MIC in <span><em>Staphylococcus cohnii</em></span> after <em>in vitro</em> tigecycline exposure was investigated. <em>S. cohnii</em><span> 11-B-312 was exposed to incremental concentrations of tigecycline (2–32 mg/L) and the mutants growing at 8, 16 and 32 mg/L were determined by AST<span> and WGS. Copy number and relative transcription level of the </span></span><em>tet</em>(L) gene were determined by quantitative PCR. The fitness cost was evaluated by growth kinetics and competition assays. The results revealed that enhanced tigecycline MIC was identified in <em>S. cohnii</em> mutants. Copy number and relative transcription level of <em>tet</em><span>(L) in the mutants increased 8-, 20-, and 23-fold and 20-, 34-, and 39-fold in the presence of 8, 16, and 32 mg/L tigecycline, respectively. The read-mapping depth ratio analysis indicated that a multidrug resistance region carrying the </span><em>tet</em>(L) variant has a gradually increased copy number, correlating with the tigecycline selection pressure. <em>S. cohnii</em><span> strain 11-B-312_32 had a fitness cost, and enhanced tigecycline MIC can revert to the initial level in the absence of tigecycline. In summary, enhanced tigecycline MIC develops with extensive amplification of an IS</span><em>257</em>-flanked <em>tet</em>(L)-carrying segment in <em>S. cohnii</em>. IS<em>257</em> seems to play a vital role in the gain and loss of the amplification product.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 8","pages":"Article 104114"},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9984927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-based analyses of family Oxalobacteraceae reveal the taxonomic classification","authors":"Tengfei Ma,&nbsp;Han Xue,&nbsp;Chungen Piao,&nbsp;Ning Jiang,&nbsp;Yong Li","doi":"10.1016/j.resmic.2023.104076","DOIUrl":"10.1016/j.resmic.2023.104076","url":null,"abstract":"<div><p>Family <span><em>Oxalobacteraceae</em></span> is known for the indicator of bacterial diversity in the environment and many of which are important beneficial bacteria. Previous studies on the taxonomic structure of family <em>Oxalobacteraceae</em><span> mostly relied on 16S rRNA gene analysis, or core-genome phylogeny of a limited number of species and resulted in taxonomic confusion within several genera. Developments in sequencing technologies have allowed more genome sequences to be obtained, enabling the revision of family </span><em>Oxalobacteraceae</em><span><span>. Here, we report a comprehensive analysis of phylogenomic trees, concatenated protein and up-to-date bacterial core gene </span>phylogenetic trees, and genomic metrics for genus demarcation on 135 genomes of </span><em>Oxalobacteraceae</em> species to elucidate their interrelationships. Following this framework for classification of species in family <em>Oxalobacteraceae</em><span>, all the proposed genera formed monophyletic lineages in the phylogenomic trees and could also be clearly separated from others in the genomic similarity indexes of average amino acid identity, percentage of conserved proteins and core-proteome average amino acid identity.</span></p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 7","pages":"Article 104076"},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10616052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corynebacterium ramonii sp. nov., a novel toxigenic member of the Corynebacterium diphtheriae species complex","authors":"Chiara Crestani ,&nbsp;Gabriele Arcari ,&nbsp;Annie Landier ,&nbsp;Virginie Passet ,&nbsp;Dorian Garnier ,&nbsp;Sylvie Brémont ,&nbsp;Nathalie Armatys ,&nbsp;Annick Carmi-Leroy ,&nbsp;Julie Toubiana ,&nbsp;Edgar Badell ,&nbsp;Sylvain Brisse","doi":"10.1016/j.resmic.2023.104113","DOIUrl":"10.1016/j.resmic.2023.104113","url":null,"abstract":"<div><p>The <span><em>Corynebacterium</em><em> diphtheriae</em></span> species complex comprises seven bacterial species, including <span><em>Corynebacterium ulcerans</em></span><span>, a zoonotic pathogen from multiple animal species. In this work, we characterise phenotypically and genotypically isolates belonging to two </span><em>C. ulcerans</em><span> lineages<span>. Results from phylogenetic analyses, </span></span><em>in silico</em> DNA–DNA hybridization (DDH) and MALDI-TOF spectra differentiate lineage 2 from <em>C. ulcerans</em> lineage 1, which, together with their distinct transmission dynamics (probable human-to-human vs animal-to-human), indicates that lineage 2 is a separate <em>Corynebacterium</em> species, which we propose to name <em>Corynebacterium ramonii</em>. This species is of particular medical interest considering that its human-to-human transmission is likely, and that some <em>C. ramonii</em> isolates carry the diphtheria toxin gene.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 7","pages":"Article 104113"},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10320457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus pfraonensis sp. nov., a new strain isolated from a probiotic feed additive with low cytotoxicity and antimicrobial activity","authors":"Fengru Deng ,&nbsp;Yunsheng Chen ,&nbsp;Huiying Xiao ,&nbsp;Chuying Yao ,&nbsp;Jiaqi Chen ,&nbsp;Yuanting Zhao ,&nbsp;Yapei Jiang ,&nbsp;Jiahang Chen ,&nbsp;Yiqun Deng ,&nbsp;Jikai Wen","doi":"10.1016/j.resmic.2023.104090","DOIUrl":"10.1016/j.resmic.2023.104090","url":null,"abstract":"<div><p><span>Probiotic products containing living microorganisms are gaining popularity, increasing the importance of their taxonomic status. A </span><span><em>Bacillus</em></span>-like isolate, 70 b, cultured from a probiotic feed additive, was ambiguity in taxonomic assignment and could be a novel member of <span><em>Bacillus cereus</em></span><span><span> group. The results of colony and cellular morphology, physiological and biochemical analysis mainly including growth performance, carbon source utilization, and rMLST<span> and MLST were not conclusive. Fatty acids profile and molecular genetic analysis especially ANI, DDH, and core genome SNPs-based </span></span>phylogenetic tree confirmed 70 b as one novel species of </span><em>B. cereus</em> group and proposed as <em>Bacillus pfraonensis</em> sp. nov. Comparative genomic analysis revealed the genetic differences between 70 b and other species of <em>B. cereus</em><span><span> group. Pseudomycoicidin was identified in 70 b. 70 b was active against multidrug-resistant pathogenic strains MRSA. The findings support 70 b is a novel species with low cytotoxicity and </span>antimicrobial activity, and provides a better understanding of its unique characteristics and probiotic potential, and exploration of bioactive potential.</span></p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 7","pages":"Article 104090"},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10270906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of the optrA gene among Streptococcus suis isolates from diseased pigs and identification of a novel integrative conjugative element ICESsu988S","authors":"Junkai Zhang ,&nbsp;Yingying Yang ,&nbsp;Huarun Sun ,&nbsp;Xingwei Luo ,&nbsp;Xiaodie Cui ,&nbsp;Qingqing Miao ,&nbsp;Dandan He ,&nbsp;Jinfeng Zhao ,&nbsp;Fengbin Yan ,&nbsp;Yushan Pan ,&nbsp;Yajun Zhai ,&nbsp;Gongzheng Hu","doi":"10.1016/j.resmic.2023.104078","DOIUrl":"10.1016/j.resmic.2023.104078","url":null,"abstract":"<div><p>Aim of this study was to investigate the prevalence and genetic environment of the oxazolidinone resistance gene <em>optrA</em> in <span><em>Streptococcus suis</em></span> (<em>S. suis</em>) isolates from diseased pigs in China. A total of 178 <em>S. suis</em> isolates were screened for the <em>optrA</em> gene by PCR. The phenotypes and genotypes of <em>optrA</em><span>-positive isolates were investigated by antimicrobial susceptibility<span><span> testing, core genome Multilocus Sequence Typing (cgMLST), capsular </span>serotypes determination and whole-genome sequencing (WGS). Fifty-one (28.7%) </span></span><em>S. suis</em> isolates were positive for <em>optrA</em><span>. Phylogenetic analysis indicated that the spread of the </span><em>optrA</em> among <em>S. suis</em> isolates was primarily due to horizontal transfer. Analysis of <em>S. suis</em> serotypes from diseased pigs revealed substantial diversity. The genetic environment of <em>optrA</em> was complex and diverse and could be divided into 12 different types. Interestingly, we identified a novel integrative and conjugative element ICE<em>Ssu</em>988S, carrying <em>optrA</em> and <em>erm</em>(T) genes. This is to the best of our knowledge the first report of the <em>optrA</em> and <em>erm</em>(T) co-located on an ICE in <em>S. suis</em>. Our results showed a high prevalence of <em>optrA</em> gene in <em>S. suis</em> isolates in China. Further research is needed to evaluate the importance of ICEs, as they horizontally propagate important clinical resistance genes.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 7","pages":"Article 104078"},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10279391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genomic approach of antimicrobial resistance of Salmonella Typhimurium isolates from guinea pigs in Lima, Peru","authors":"Raquel Hurtado ,&nbsp;Debmalya Barh ,&nbsp;Luís Cláudio Lima de Jesus ,&nbsp;Marcus Vinicius Canário Viana ,&nbsp;Sandeep Tiwari ,&nbsp;Flávia Figueira Aburjaile ,&nbsp;Dennis E. Carhuaricra Huaman ,&nbsp;Bertram Brenig ,&nbsp;Lenin Maturrano Hernández ,&nbsp;Vasco Azevedo","doi":"10.1016/j.resmic.2023.104086","DOIUrl":"10.1016/j.resmic.2023.104086","url":null,"abstract":"<div><p><em>Salmonella</em> Typhimurium is an important agent of foodborne diseases. In Peru, the emergence of multidrug-resistant isolates of <em>S.</em><span> Typhimurium from the food chain could be linked to guinea pig farming as a potential reservoir and their uncontrolled antibiotic treatment against salmonellosis. In this study, we performed the sequencing, genomic diversity, and characterization of resistance elements transmitted by isolates from farm and meat guinea pigs. The genomic diversity and antimicrobial resistance of </span><em>S.</em><span><span> Typhimurium isolates were performed using nucleotide similarity, cgMLST, serotyping, phylogenomic<span> analyses, and characterization of resistance plasmids. We found at least four populations of isolates from farm guinea pigs and four populations from meat guinea pigs without finding isolated transmission between both resources. Genotypic resistance to antibiotics was observed in at least 50% of the isolates. Among the farm guinea pig isolates, ten were found to be resistant to nalidixic acid, and two isolates exhibited </span></span>multidrug resistance to aminoglycosides, tetracycline-fluoroquinolone (carrying </span><em>strA-strB-tetA-tetB</em> genes and <em>gyrA</em> S83F mutation), or trimethoprim-sulfonamide (carrying <em>AaadA1-drfA15-sul1</em> gene<em>s</em><span><span>). Additionally, two isolates from the meat source were resistant to fluoroquinolones (one of which had enrofloxacin resistance). The transmissible resistance plasmids with insertion sequences (IS) such as IncI-gamma-K1-ISE3-IS6, IncI1-I (alpha)-IS21-Tn10, and Col (pHAD28) were commonly found in isolates belonging to the HC100-9757 cluster from both guinea pigs and human hosts. Altogether, our work provides resistance determinants profiles and Salmonella sp. circulating </span>lineages using WGS data that can promote better sanitary control and adequate antimicrobial prescription.</span></p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 7","pages":"Article 104086"},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9620525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The periplasmic coiled coil formed by the assembly platform proteins PulL and PulM is critical for function of the Klebsiella type II secretion system","authors":"Yuanyuan Li,&nbsp;Javier Santos-Moreno ,&nbsp;Olivera Francetic","doi":"10.1016/j.resmic.2023.104075","DOIUrl":"10.1016/j.resmic.2023.104075","url":null,"abstract":"<div><p>Bacteria use type II secretion systems (T2SS) to secrete to their surface folded proteins that confer diverse functions, from nutrient acquisition to virulence. In the <em>Klebsiella</em> species, T2SS-mediated secretion of pullulanase (PulA) requires assembly of a dynamic filament called the endopilus. The inner membrane assembly platform (AP) subcomplex is essential for endopilus assembly and PulA secretion. AP components PulL and PulM interact with each other through their C-terminal globular domains and transmembrane segments. Here, we investigated the roles of their periplasmic helices, predicted to form a coiled coil, in assembly and function of the PulL–PulM complex. PulL and PulM variants lacking these periplasmic helices were defective for interaction in the bacterial two-hybrid (BACTH) assay. Their functions in PulA secretion and assembly of PulG subunits into endopilus filaments were strongly reduced. Interestingly, deleting the cytoplasmic peptide of PulM nearly abolished the function of variant PulMΔN and its interaction with PulG, but not with PulL, in the BACTH assay. Nevertheless, PulL was specifically proteolyzed in the presence of the PulMΔN variant, suggesting that PulM N-terminal peptide stabilizes PulL in the cytoplasm. We discuss the implications of these results for the T2S endopilus and type IV pilus assembly mechanisms.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 7","pages":"Article 104075"},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10331112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DnaJ, a heat shock protein 40 family member, is essential for the survival and virulence of plant pathogenic Pseudomonas cichorii JBC1","authors":"Anh Tran Thi Ngoc ,&nbsp;Khanh Nguyen Van ,&nbsp;Yong Hoon Lee","doi":"10.1016/j.resmic.2023.104094","DOIUrl":"10.1016/j.resmic.2023.104094","url":null,"abstract":"<div><p><span>Bacterial plant pathogens must cope with various environmental conditions and defenses from their hosts for colonization and infection. Heat shock proteins (HSPs) play critical roles in a variety of cellular processes, such as the maintenance of cellular homeostasis in response to environmental stress. However, the significance of HSP40 family protein DnaJ in virulence of plant pathogenic bacteria has not yet been explored. To elucidate the function of DnaJ in </span><span><em>Pseudomonas</em><em> cichorii</em></span> JBC1 (PcJBC1) virulence, we generated <em>dnaJ</em>-deficient (JBC1^ΔdnaJ) mutant using CRISPR-CAS9. The disease severity by JBC1^ΔdnaJ was significantly reduced compared with wild-type (WT) and <em>dnaJ</em>-complemented (JBC1^Δdna^J<span> + pdnaJ) strain. The defect of DnaJ suppressed siderophore production, extracellular DNA (eDNA) release, biofilm formation, and swarming motility and made the strain sensitive to stresses such as heat and H</span>₂O₂. The supplementation of eDNA recovered the amount of biofilm formation by JBC1^ΔdnaJ. Our results indicate that DnaJ is a key player in the survival and colonization of bacterial plant pathogens on plant surfaces as well as bacterial responses to abiotic and biotic stresses, which are determinative to cause disease. These findings can broaden our understanding of plant and bacterial pathogen interactions.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 7","pages":"Article 104094"},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10271358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the efficacy of endolysins and membrane permeabilizers against Vibrio parahaemolyticus in marine conditions","authors":"Lina Angélica Zermeño-Cervantes ,&nbsp;Sergio Francisco Martínez-Díaz ,&nbsp;Alberto Antony Venancio-Landeros ,&nbsp;César Salvador Cardona-Félix","doi":"10.1016/j.resmic.2023.104104","DOIUrl":"10.1016/j.resmic.2023.104104","url":null,"abstract":"<div><p><span>Endolysins have garnered significant attention as a potential alternative to antibiotics in aquaculture, mainly for combating </span><span><em>Vibrio</em></span><span><span> spp., Gram-negative pathogens responsible for infectious outbreaks. However, endolysin effectiveness against Gram-negative bacteria is limited due to the outer membrane's poor permeability. The combat against marine pathogens poses an additional challenge of finding endolysins that retain their activity in high </span>ionic strength conditions. Thus, this study aimed to demonstrate that certain endolysins retain muralytic activity in seawater and also evaluated outer membrane permeabilizers as endolysin adjuvants. The effectiveness of KZ144 and LysPA26 endolysins, along with EDTA and oregano essential oil, was evaluated against </span><span><em>Vibrio parahaemolyticus</em></span> ATCC-17802 in natural seawater. Results revealed the muralytic activity of both endolysins in seawater. However, the endolysins appeared to counteract the permeabilizers' effect during the initial bactericidal assays. Further investigations revealed that the observed effect was not antagonistic. After the permeabilizer action, <em>V. parahaemolyticus</em><span> likely used endolysins as a growth substrate. Endolysins may not play an indifferent role if they fail to exert a bactericidal effect. Instead, they can serve as a substrate for fast-growing bacteria, such as </span><em>V. parahaemolyticus</em>, increasing bacterial density. It should be considered a potential drawback of endolysins' proteinaceous nature as bactericidal agents.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 7","pages":"Article 104104"},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10280411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond membrane components: uncovering the intriguing world of fungal sphingolipid synthesis and regulation","authors":"Sana Akhtar Usmani ,&nbsp;Mohit Kumar ,&nbsp;Khushboo Arya ,&nbsp;Basharat Ali ,&nbsp;Nitin Bhardwaj ,&nbsp;Naseem Akhtar Gaur ,&nbsp;Rajendra Prasad ,&nbsp;Ashutosh Singh","doi":"10.1016/j.resmic.2023.104087","DOIUrl":"10.1016/j.resmic.2023.104087","url":null,"abstract":"<div><p>Sphingolipids (SLs) are essential to fungal survival and represent a major class of structural and signaling lipids. Unique SL structures and their biosynthetic enzymes in filamentous fungi<span> make them an ideal drug target. Several studies have contributed towards the functional characterization of specific SL metabolism genes, which have been complemented by advanced lipidomics methods which allow accurate identification and quantification of lipid structures and pathway mapping. These studies have provided a better understanding of SL biosynthesis, degradation and regulation networks in filamentous fungi, which are discussed and elaborated here.</span></p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 7","pages":"Article 104087"},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10633121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}