Research in microbiology最新文献_第8页

Programmed cell death in Xanthomonas axonopodis pv. glycines is associated with modulation of gene expression resulting in altered states of motility, biofilm and virulence 轴足黄单胞菌的程序性细胞死亡。甘氨酸与基因表达的调节有关，导致运动性、生物膜和毒力状态的改变。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104137

Nilantana C. Bandyopadhyay , Satyendra Gautam

{"title":"Programmed cell death in Xanthomonas axonopodis pv. glycines is associated with modulation of gene expression resulting in altered states of motility, biofilm and virulence","authors":"Nilantana C. Bandyopadhyay , Satyendra Gautam","doi":"10.1016/j.resmic.2023.104137","DOIUrl":"10.1016/j.resmic.2023.104137","url":null,"abstract":"<div>One of the foremost report of apoptosis-like programmed cell death (PCD) came from Xanthomonas axonopodis pv. glycines (Xag), which displayed rapid post-exponential cell death in PCD inducing media (PIM) but not in a non-inducing media (PNIM). The current study aims to decipher for the first time, the advantages of the existence of PCD in this phytopathogenic microorganism. Analysis of RNA-seq under inducing and non-inducing conditions, revealed differential expression of a number of genes related to key physiology of Xag, such as, motility, xanthan biosynthesis and export as well as virulence. A PCD negative mutant Xag M42 displayed diminished virulence and a contrasting transcriptome pattern. In vitro experiments revealed that under PCD inducing condition, Xag produced negligible xanthan gum as well as extracellular amylase, displayed enhanced swarming motility, released copious e-DNA and formed scanty biofilm. Lack of ‘diffusible signalling factor’ production was eliminated as possible reason for PCD-induction. Altogether, it appears that, in planta existence of the pathogen metabolically resembles PNIM, and on being transferred to PIM, the cells experience oxidative stress and circumvents it by adopting PCD as an altruistic response. Survival of the remaining population is encouraged by upregulating motility, detachment from the fragile biofilm to achieve dispersal.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 8","pages":"Article 104137"},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10269190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

trans-translation system is important for maintaining genome integrity during DNA damage in bacteria 反翻译系统在细菌DNA损伤过程中对维持基因组完整性非常重要。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104136

Nagarajan T , Sutharsan Govindarajan , M. Hussain Munavar

{"title":"trans-translation system is important for maintaining genome integrity during DNA damage in bacteria","authors":"Nagarajan T , Sutharsan Govindarajan , M. Hussain Munavar","doi":"10.1016/j.resmic.2023.104136","DOIUrl":"10.1016/j.resmic.2023.104136","url":null,"abstract":"<div>DNA integrity in bacteria is regulated by various factors that act on the DNA. trans-translation has previously been shown to be important for the survival of Escherichia coli cells exposed to certain DNA-damaging agents. However, the mechanisms underlying this sensitivity are poorly understood. In this study, we explored the involvement of the trans-translation system in the maintenance of genome integrity using various DNA-damaging agents and mutant backgrounds. Relative viability assays showed that SsrA-defective cells were sensitive to DNA-damaging agents, such as nalidixic acid (NA), ultraviolet radiation (UV), and methyl methanesulfonate (MMS). The viability of SsrA-defective cells was rescued by deleting sulA, although the expression of SulA was not more pronounced in SsrA-defective cells than in wild-type cells. Live cell imaging using a Gam-GFP fluorescent reporter showed increased double-strand breaks (DSBs) in SsrA-defective cells during DNA damage. We also showed that the ribosome rescue function of SsrA was sufficient for DNA damage tolerance. DNA damage sensitivity can be alleviated by partial uncoupling of transcription and translation by using sub-lethal concentrations of ribosome inhibiting antibiotic (tetracycline) or by mutating the gene coding for RNase H (rnhA). Taken together, our results highlight the importance of trans-translation system in maintaining genome integrity and bacterial survival during DNA damage.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 8","pages":"Article 104136"},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10201239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Skin microbiome bacteria enriched following long sun exposure can reduce oxidative damage 长时间暴露在阳光下后富含的皮肤微生物组细菌可以减少氧化损伤。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104138

Nurit Harel , Navit Ogen-Shtern , Leah Reshef , Dvora Biran , Eliora Z. Ron , Uri Gophna

{"title":"Skin microbiome bacteria enriched following long sun exposure can reduce oxidative damage","authors":"Nurit Harel , Navit Ogen-Shtern , Leah Reshef , Dvora Biran , Eliora Z. Ron , Uri Gophna","doi":"10.1016/j.resmic.2023.104138","DOIUrl":"10.1016/j.resmic.2023.104138","url":null,"abstract":"<div>Sun exposure is harmful to the skin and increases the risk of skin aging and skin cancer. Here we examined the effects of daily exposure to sun radiation on the skin microbiome in order to determine whether skim microbiome bacteria can contribute to protection from solar damage. Skin swabs were collected from ten lifeguards before and after the summer to analyse the skin microbiome. The results indicate that specific skin microbiome bacteria were enriched following the seasonal sun exposure. Especially interesting were two bacterial families - Sphingomonas and Erythrobacteraceae – which may have the ability to protect against UV radiation as they produce potentially protective compounds. We concentrated on a Sphingomonas strain and could show that it was highly resistant to UV irradiation and was able to reduce reactive oxygen species levels in human keratinocytes. These results provide a proof-of-concept for the role of the skin microbiome in protection from solar radiation.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 8","pages":"Article 104138"},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10309411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The importance of the PapR7 C-terminus and amide protons in mediating quorum sensing in Bacillus cereus PapR7 C末端和酰胺质子在介导Bacilluscereus群体感应中的重要性。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104139

Michael Gorgan , Shahar Vanunu Ofri , Emilee R. Engler , Avishag Yehuda , Elizabeth Hutnick , Zvi Hayouka , Michael A. Bertucci

{"title":"The importance of the PapR7 C-terminus and amide protons in mediating quorum sensing in Bacillus cereus","authors":"Michael Gorgan , Shahar Vanunu Ofri , Emilee R. Engler , Avishag Yehuda , Elizabeth Hutnick , Zvi Hayouka , Michael A. Bertucci","doi":"10.1016/j.resmic.2023.104139","DOIUrl":"10.1016/j.resmic.2023.104139","url":null,"abstract":"<div>The opportunistic human pathogen Bacillus cereus controls the expression of key infection-promoting phenotypes using bacterial quorum sensing (QS). QS signal transduction within the species is controlled by an autoinducing peptide, PapR7, and its cognate receptor, PlcR, indicating that the PlcR:PapR interface is a prime target for QS inhibitor development. The C-terminal region of the peptide (PapR7; ADLPFEF) has been successfully employed as a scaffold to develop potent QS modulators. Despite the noted importance of the C-terminal carboxylate and amide protons in crystallographic data, their role in QS activity has yet to be explored. In this study, an N-methyl scan of PapR7 was conducted in conjunction with a C-terminal modification of previously identified B. cereus QS inhibitors. The results indicate that the amide proton at Glu6 and the C-terminal carboxylate are important for effective QS inhibition of the PlcR regulon. Through β-galactosidase and hemolysis assays, a series of QS inhibitors were discovered, including several capable of inhibiting QS with nanomolar potency. These inhibitors, along with the structure–activity data reported, will serve as valuable tools for disrupting the B. cereus QS pathway towards developing novel anti-infective strategies.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 8","pages":"Article 104139"},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41132503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heat shock response in Sulfolobus acidocaldarius and first implications for cross-stress adaptation 嗜酸硫酸藻的热休克反应和交叉应激适应的初步意义。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104106

Arghya Bhowmick , Koustav Bhakta , Mousam Roy , Sayandeep Gupta , Jagriti Das , Shirsha Samanta , Somi Patranabis , Abhrajyoti Ghosh

{"title":"Heat shock response in Sulfolobus acidocaldarius and first implications for cross-stress adaptation","authors":"Arghya Bhowmick , Koustav Bhakta , Mousam Roy , Sayandeep Gupta , Jagriti Das , Shirsha Samanta , Somi Patranabis , Abhrajyoti Ghosh","doi":"10.1016/j.resmic.2023.104106","DOIUrl":"10.1016/j.resmic.2023.104106","url":null,"abstract":"<div>Sulfolobus acidocaldarius, a thermoacidophilic crenarchaeon, frequently encounters temperature fluctuations, oxidative stress, and nutrient limitations in its environment. Here, we employed a high-throughput transcriptomic analysis to examine how the gene expression of S. acidocaldarius changes when exposed to high temperatures (92 °C). The data obtained was subsequently validated using quantitative reverse transcription-PCR (qRT-PCR) analysis. Our particular focus was on genes that are involved in the heat shock response, type-II Toxin-Antitoxin systems, and putative transcription factors. To investigate how S. acidocaldarius adapts to multiple stressors, we assessed the expression of these selected genes under oxidative and nutrient stresses using qRT-PCR analysis. The results demonstrated that the gene thβ encoding the β subunit of the thermosome, as well as hsp14 and hsp20, play crucial roles in the majority of stress conditions. Furthermore, we observed overexpression of at least eight different TA pairs belonging to the type II TA systems under all stress conditions. Additionally, four common transcription factors: FadR, TFEβ, CRISPR loci binding protein, and HTH family protein were consistently overexpressed across all stress conditions, indicating their significant role in managing stress. Overall, this work provides the first insight into molecular players involved in the cross-stress adaptation of S. acidocaldarius.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 8","pages":"Article 104106"},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10247814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Leptospiral cell wall hydrolase (LIC_10271) binding peptidoglycan, lipopolysaccharide, and laminin and the protein show LysM and M23 domains are co-existing in pathogenic species 钩端螺旋体细胞壁水解酶（LIC_10271）结合肽聚糖、脂多糖和层粘连蛋白，该蛋白显示LysM和M23结构域在致病物种中共存。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104107

Abhijit Sarma , Gunasekaran Dhandapani , Homen Phukan , Prasun Kumar Bhunia , Arun Kumar De , Debasis Bhattacharya , T. Jebasingh , Madathiparambil G. Madanan

{"title":"Leptospiral cell wall hydrolase (LIC_10271) binding peptidoglycan, lipopolysaccharide, and laminin and the protein show LysM and M23 domains are co-existing in pathogenic species","authors":"Abhijit Sarma , Gunasekaran Dhandapani , Homen Phukan , Prasun Kumar Bhunia , Arun Kumar De , Debasis Bhattacharya , T. Jebasingh , Madathiparambil G. Madanan","doi":"10.1016/j.resmic.2023.104107","DOIUrl":"10.1016/j.resmic.2023.104107","url":null,"abstract":"<div>Leptospirosis, a global reemerging zoonosis caused by the spirochete Leptospira, has severe human and veterinary implications. Cell wall hydrolase (LIC_10271) with LytM (peptidase M23) and LysM domains are found to be associated with various pathogenic bacteria. These domains regulate effects on extracellular matrix and biofilm components, which promote cell wall remodeling and pathogen dissemination in the host. In this study, we present the cloning, expression, purification, and characterization of LIC_10271. To determine the localization of LIC_10271 within the inner membrane of Leptospira, Triton X-114 subcellular fractionation and immunoblot studies were performed. Furthermore, r-LIC_10271 binds with peptidoglycan, lipopolysaccharide, and laminin in a dose-dependent manner. Analysis of the signal peptide, M23, and LysM domains revealed conservation primarily within the P1 group of Leptospira, which encompasses the most pathogenic species. Moreover, the presence of native-LIC_10271 in the inner membrane and the distribution of M23 and LysM domains across pathogenic strains indicates their potential involvement in the interaction between the host and Leptospira.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 8","pages":"Article 104107"},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9951818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deficient Pseudomonas aeruginosa in MlaA/VacJ outer membrane lipoprotein shows decrease in rhamnolipids secretion, motility, and biofilm formation, and increase in fluoroquinolones susceptibility and innate immune response MlaA/VacJ外膜脂蛋白中的铜绿假单胞菌缺陷显示鼠李糖脂分泌、运动性和生物膜形成减少，氟喹诺酮类药物敏感性和先天免疫反应增加。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104132

M. Kaur , J.M. Buyck , F. Goormaghtigh , J.-L. Decout , N. Mozaheb , M.-P. Mingeot-Leclercq

{"title":"Deficient Pseudomonas aeruginosa in MlaA/VacJ outer membrane lipoprotein shows decrease in rhamnolipids secretion, motility, and biofilm formation, and increase in fluoroquinolones susceptibility and innate immune response","authors":"M. Kaur , J.M. Buyck , F. Goormaghtigh , J.-L. Decout , N. Mozaheb , M.-P. Mingeot-Leclercq","doi":"10.1016/j.resmic.2023.104132","DOIUrl":"10.1016/j.resmic.2023.104132","url":null,"abstract":"<div>Pseudomonas aeruginosa, a Gram-negative bacterium that causes severe hospital acquired infections poses threat by its ability for adaptation to various growth modes and environmental conditions and by its intrinsic resistance to antibiotics. The latter is mainly due to the outer membrane (OM) asymmetry which is maintained by the Mla pathway resulting in the retrograde transport of glycerophospholipids from the OM to the inner membrane. It comprises six Mla proteins, including MlaA, an OM lipoprotein involved in the removal of glycerophospholipids mislocalized at the outer leaflet of OM.To investigate the role of P. aeruginosa OM asymmetry especially MlaA, this study investigated the effect of mlaA deletion on (i) the susceptibility to antibiotics, (ii) the secretion of virulence factors, the motility, biofilm formation, and (iii) the inflammatory response.mlaA deletion in P. aeruginosa ATCC27853 results in phenotypic changes including, an increase in fluoroquinolones susceptibility and in PQS (Pseudomonas Quinolone Signal) and TNF-α release and a decrease in rhamnolipids secretion, motility and biofilm formation.Investigating how the mlaA knockout impacts on antibiotic susceptibility, bacterial virulence and innate immune response will help to elucidate the biological significance of the Mla system and contribute to the understanding of MlaA in P. aeruginosa OM asymmetry.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 8","pages":"Article 104132"},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0923250823001079/pdfft?md5=0ddbed0060c793d80f891fde818c0b30&pid=1-s2.0-S0923250823001079-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10146831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding the chromosome-scale genome of the nutrient-rich Agaricus subrufescens: a resource for fungal biology and biotechnology 解码营养丰富的亚陆生蘑菇的染色体规模基因组：真菌生物学和生物技术的资源。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104116

Carlos Godinho de Abreu , Luiz Fernando Wurdig Roesch , Fernando Dini Andreote , Saura Rodrigues Silva , Tatiana Silveira Junqueira de Moraes , Diego Cunha Zied , Félix Gonçalves de Siqueira , Eustáquio Souza Dias , Alessandro M. Varani , Victor Satler Pylro

{"title":"Decoding the chromosome-scale genome of the nutrient-rich Agaricus subrufescens: a resource for fungal biology and biotechnology","authors":"Carlos Godinho de Abreu , Luiz Fernando Wurdig Roesch , Fernando Dini Andreote , Saura Rodrigues Silva , Tatiana Silveira Junqueira de Moraes , Diego Cunha Zied , Félix Gonçalves de Siqueira , Eustáquio Souza Dias , Alessandro M. Varani , Victor Satler Pylro","doi":"10.1016/j.resmic.2023.104116","DOIUrl":"10.1016/j.resmic.2023.104116","url":null,"abstract":"<div>Agaricus subrufescens, also known as the “sun mushroom,” has significant nutritional and medicinal value. However, its short shelf life due to the browning process results in post-harvest losses unless it's quickly dehydrated. This restricts its availability to consumers in the form of capsules. A genome sequence of A. subrufescens may lead to new cultivation alternatives or the application of gene editing strategies to delay the browning process. We assembled a chromosome-scale genome using a hybrid approach combining Illumina and Nanopore sequencing. The genome was assembled into 13 chromosomes and 31 unplaced scaffolds, totaling 44.5 Mb with 96.5% completeness and 47.24% GC content. 14,332 protein-coding genes were identified, with 64.6% of the genome covered by genes and 23.41% transposable elements. The mitogenome was circularized and encoded fourteen typical mitochondrial genes. Four polyphenol oxidase (PPO) genes and the Mating-type locus were identified. Phylogenomic analysis supports the placement of A. subrufescens in the Agaricomycetes clade. This is the first available genome sequence of a strain of the “sun mushroom.” Results are available through a Genome Browser (https://plantgenomics.ncc.unesp.br/gen.php?id=Asub) and can support further fungal biological and genomic studies.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 8","pages":"Article 104116"},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10044329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dethiobiotin uptake and utilization by bacteria possessing bioYB operon 具有生物YB操纵子的细菌对脱硫生物素的吸收和利用。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104131

Tomoki Ikeda , Tetsuhiro Ogawa , Toshihiro Aono

{"title":"Dethiobiotin uptake and utilization by bacteria possessing bioYB operon","authors":"Tomoki Ikeda , Tetsuhiro Ogawa , Toshihiro Aono","doi":"10.1016/j.resmic.2023.104131","DOIUrl":"10.1016/j.resmic.2023.104131","url":null,"abstract":"<div>Biotin is an essential vitamin for all organisms. Some bacteria cannot synthesize biotin and live by acquiring biotin from the environment. Bacterial biotin transporters (BioY) are classified into three mechanistic types. The first forms the BioMNY complex with ATPase (BioM) and transmembrane protein (BioN). The second relies on a promiscuous energy coupling module. The third functions independently. One-third of bioY genes spread in bacteria cluster with bioM and bioN on the genomes, and the rest does not. Interestingly, some bacteria have the bioY gene clustering with bioB gene, which encodes biotin synthase, an enzyme that converts dethiobiotin to biotin, on their genome. This bioY-bioB cluster is observed even though these bacteria cannot synthesize biotin. Azorhizobium caulinodans ORS571, a rhizobium of tropical legume Sesbania rostrata, is one of such bacteria. In this study using this bacterium, we demonstrated that the BioY linked to BioB could transport not only biotin but also dethiobiotin, and the combination of BioY and BioB contributed to the growth of A. caulinodans ORS571 in a biotin-deficient but dethiobiotin-sufficient environment. We propose that such environment universally exists in the natural world, and the identification of such environment will be a new subject in the field of microbial ecology.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 8","pages":"Article 104131"},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10484351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modification of the second PEP4-allele facilitates an industrial Saccharomyces cerevisiae to tolerate tartaric acid stress 第二个PEP4等位基因的修饰促进工业酿酒酵母耐受酒石酸应激。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104109

Hongbo Zhang, Xiaomei Yang, Chi Shen, Jianqiu Sun, Yuhang Lu, Wanting Hu, Hongfei Yao, Wenhao Zhao

{"title":"Modification of the second PEP4-allele facilitates an industrial Saccharomyces cerevisiae to tolerate tartaric acid stress","authors":"Hongbo Zhang, Xiaomei Yang, Chi Shen, Jianqiu Sun, Yuhang Lu, Wanting Hu, Hongfei Yao, Wenhao Zhao","doi":"10.1016/j.resmic.2023.104109","DOIUrl":"10.1016/j.resmic.2023.104109","url":null,"abstract":"<div>The practical significance of constructing robust industrial production strains against organic acid stress lies not only in improving fermentation efficiency but also in reducing manufacturing costs. In a previous study, we constructed an industrial Saccharomyces cerevisiae strain by modifying another PEP4-allele of a mutant that already had one PEP4-allele disrupted. This modification enhanced cellular tolerance to citric acid stress during growth. Unlike citric acid, which S. cerevisiae can consume, tartaric acid is often added to grape must during winemaking to increase total acidity and is not metabolizable. The results of the present study indicate that the modification of the second PEP4-allele improves the cellular tolerance of the strain with one PEP4-allele disrupted against tartaric acid stress during growth and contributes to maintaining intracellular pH homeostasis in cells subjected to tartaric acid stress. Moreover, under tartaric acid stress, a significant improvement in glucose-ethanol conversion performance, conferred by the modification of the second PEP4-allele, was observed. This study not only broadens our understanding of the role of the PEP4-allele in cellular regulation but also provides a prospective approach to reducing the concentration of sulfur dioxide used in winemaking.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 8","pages":"Article 104109"},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9951819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0