Research in microbiology最新文献

{"title":"Prevalence of the optrA gene among Streptococcus suis isolates from diseased pigs and identification of a novel integrative conjugative element ICESsu988S","authors":"Junkai Zhang ,&nbsp;Yingying Yang ,&nbsp;Huarun Sun ,&nbsp;Xingwei Luo ,&nbsp;Xiaodie Cui ,&nbsp;Qingqing Miao ,&nbsp;Dandan He ,&nbsp;Jinfeng Zhao ,&nbsp;Fengbin Yan ,&nbsp;Yushan Pan ,&nbsp;Yajun Zhai ,&nbsp;Gongzheng Hu","doi":"10.1016/j.resmic.2023.104078","DOIUrl":"10.1016/j.resmic.2023.104078","url":null,"abstract":"<div><p>Aim of this study was to investigate the prevalence and genetic environment of the oxazolidinone resistance gene <em>optrA</em> in <span><em>Streptococcus suis</em></span> (<em>S. suis</em>) isolates from diseased pigs in China. A total of 178 <em>S. suis</em> isolates were screened for the <em>optrA</em> gene by PCR. The phenotypes and genotypes of <em>optrA</em><span>-positive isolates were investigated by antimicrobial susceptibility<span><span> testing, core genome Multilocus Sequence Typing (cgMLST), capsular </span>serotypes determination and whole-genome sequencing (WGS). Fifty-one (28.7%) </span></span><em>S. suis</em> isolates were positive for <em>optrA</em><span>. Phylogenetic analysis indicated that the spread of the </span><em>optrA</em> among <em>S. suis</em> isolates was primarily due to horizontal transfer. Analysis of <em>S. suis</em> serotypes from diseased pigs revealed substantial diversity. The genetic environment of <em>optrA</em> was complex and diverse and could be divided into 12 different types. Interestingly, we identified a novel integrative and conjugative element ICE<em>Ssu</em>988S, carrying <em>optrA</em> and <em>erm</em>(T) genes. This is to the best of our knowledge the first report of the <em>optrA</em> and <em>erm</em>(T) co-located on an ICE in <em>S. suis</em>. Our results showed a high prevalence of <em>optrA</em> gene in <em>S. suis</em> isolates in China. Further research is needed to evaluate the importance of ICEs, as they horizontally propagate important clinical resistance genes.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10279391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genomic approach of antimicrobial resistance of Salmonella Typhimurium isolates from guinea pigs in Lima, Peru","authors":"Raquel Hurtado ,&nbsp;Debmalya Barh ,&nbsp;Luís Cláudio Lima de Jesus ,&nbsp;Marcus Vinicius Canário Viana ,&nbsp;Sandeep Tiwari ,&nbsp;Flávia Figueira Aburjaile ,&nbsp;Dennis E. Carhuaricra Huaman ,&nbsp;Bertram Brenig ,&nbsp;Lenin Maturrano Hernández ,&nbsp;Vasco Azevedo","doi":"10.1016/j.resmic.2023.104086","DOIUrl":"10.1016/j.resmic.2023.104086","url":null,"abstract":"<div><p><em>Salmonella</em> Typhimurium is an important agent of foodborne diseases. In Peru, the emergence of multidrug-resistant isolates of <em>S.</em><span> Typhimurium from the food chain could be linked to guinea pig farming as a potential reservoir and their uncontrolled antibiotic treatment against salmonellosis. In this study, we performed the sequencing, genomic diversity, and characterization of resistance elements transmitted by isolates from farm and meat guinea pigs. The genomic diversity and antimicrobial resistance of </span><em>S.</em><span><span> Typhimurium isolates were performed using nucleotide similarity, cgMLST, serotyping, phylogenomic<span> analyses, and characterization of resistance plasmids. We found at least four populations of isolates from farm guinea pigs and four populations from meat guinea pigs without finding isolated transmission between both resources. Genotypic resistance to antibiotics was observed in at least 50% of the isolates. Among the farm guinea pig isolates, ten were found to be resistant to nalidixic acid, and two isolates exhibited </span></span>multidrug resistance to aminoglycosides, tetracycline-fluoroquinolone (carrying </span><em>strA-strB-tetA-tetB</em> genes and <em>gyrA</em> S83F mutation), or trimethoprim-sulfonamide (carrying <em>AaadA1-drfA15-sul1</em> gene<em>s</em><span><span>). Additionally, two isolates from the meat source were resistant to fluoroquinolones (one of which had enrofloxacin resistance). The transmissible resistance plasmids with insertion sequences (IS) such as IncI-gamma-K1-ISE3-IS6, IncI1-I (alpha)-IS21-Tn10, and Col (pHAD28) were commonly found in isolates belonging to the HC100-9757 cluster from both guinea pigs and human hosts. Altogether, our work provides resistance determinants profiles and Salmonella sp. circulating </span>lineages using WGS data that can promote better sanitary control and adequate antimicrobial prescription.</span></p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9620525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DnaJ, a heat shock protein 40 family member, is essential for the survival and virulence of plant pathogenic Pseudomonas cichorii JBC1","authors":"Anh Tran Thi Ngoc ,&nbsp;Khanh Nguyen Van ,&nbsp;Yong Hoon Lee","doi":"10.1016/j.resmic.2023.104094","DOIUrl":"10.1016/j.resmic.2023.104094","url":null,"abstract":"<div><p><span>Bacterial plant pathogens must cope with various environmental conditions and defenses from their hosts for colonization and infection. Heat shock proteins (HSPs) play critical roles in a variety of cellular processes, such as the maintenance of cellular homeostasis in response to environmental stress. However, the significance of HSP40 family protein DnaJ in virulence of plant pathogenic bacteria has not yet been explored. To elucidate the function of DnaJ in </span><span><em>Pseudomonas</em><em> cichorii</em></span> JBC1 (PcJBC1) virulence, we generated <em>dnaJ</em>-deficient (JBC1^ΔdnaJ) mutant using CRISPR-CAS9. The disease severity by JBC1^ΔdnaJ was significantly reduced compared with wild-type (WT) and <em>dnaJ</em>-complemented (JBC1^Δdna^J<span> + pdnaJ) strain. The defect of DnaJ suppressed siderophore production, extracellular DNA (eDNA) release, biofilm formation, and swarming motility and made the strain sensitive to stresses such as heat and H</span>₂O₂. The supplementation of eDNA recovered the amount of biofilm formation by JBC1^ΔdnaJ. Our results indicate that DnaJ is a key player in the survival and colonization of bacterial plant pathogens on plant surfaces as well as bacterial responses to abiotic and biotic stresses, which are determinative to cause disease. These findings can broaden our understanding of plant and bacterial pathogen interactions.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10271358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the efficacy of endolysins and membrane permeabilizers against Vibrio parahaemolyticus in marine conditions","authors":"Lina Angélica Zermeño-Cervantes ,&nbsp;Sergio Francisco Martínez-Díaz ,&nbsp;Alberto Antony Venancio-Landeros ,&nbsp;César Salvador Cardona-Félix","doi":"10.1016/j.resmic.2023.104104","DOIUrl":"10.1016/j.resmic.2023.104104","url":null,"abstract":"<div><p><span>Endolysins have garnered significant attention as a potential alternative to antibiotics in aquaculture, mainly for combating </span><span><em>Vibrio</em></span><span><span> spp., Gram-negative pathogens responsible for infectious outbreaks. However, endolysin effectiveness against Gram-negative bacteria is limited due to the outer membrane's poor permeability. The combat against marine pathogens poses an additional challenge of finding endolysins that retain their activity in high </span>ionic strength conditions. Thus, this study aimed to demonstrate that certain endolysins retain muralytic activity in seawater and also evaluated outer membrane permeabilizers as endolysin adjuvants. The effectiveness of KZ144 and LysPA26 endolysins, along with EDTA and oregano essential oil, was evaluated against </span><span><em>Vibrio parahaemolyticus</em></span> ATCC-17802 in natural seawater. Results revealed the muralytic activity of both endolysins in seawater. However, the endolysins appeared to counteract the permeabilizers' effect during the initial bactericidal assays. Further investigations revealed that the observed effect was not antagonistic. After the permeabilizer action, <em>V. parahaemolyticus</em><span> likely used endolysins as a growth substrate. Endolysins may not play an indifferent role if they fail to exert a bactericidal effect. Instead, they can serve as a substrate for fast-growing bacteria, such as </span><em>V. parahaemolyticus</em>, increasing bacterial density. It should be considered a potential drawback of endolysins' proteinaceous nature as bactericidal agents.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10280411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The periplasmic coiled coil formed by the assembly platform proteins PulL and PulM is critical for function of the Klebsiella type II secretion system","authors":"Yuanyuan Li,&nbsp;Javier Santos-Moreno ,&nbsp;Olivera Francetic","doi":"10.1016/j.resmic.2023.104075","DOIUrl":"10.1016/j.resmic.2023.104075","url":null,"abstract":"<div><p>Bacteria use type II secretion systems (T2SS) to secrete to their surface folded proteins that confer diverse functions, from nutrient acquisition to virulence. In the <em>Klebsiella</em> species, T2SS-mediated secretion of pullulanase (PulA) requires assembly of a dynamic filament called the endopilus. The inner membrane assembly platform (AP) subcomplex is essential for endopilus assembly and PulA secretion. AP components PulL and PulM interact with each other through their C-terminal globular domains and transmembrane segments. Here, we investigated the roles of their periplasmic helices, predicted to form a coiled coil, in assembly and function of the PulL–PulM complex. PulL and PulM variants lacking these periplasmic helices were defective for interaction in the bacterial two-hybrid (BACTH) assay. Their functions in PulA secretion and assembly of PulG subunits into endopilus filaments were strongly reduced. Interestingly, deleting the cytoplasmic peptide of PulM nearly abolished the function of variant PulMΔN and its interaction with PulG, but not with PulL, in the BACTH assay. Nevertheless, PulL was specifically proteolyzed in the presence of the PulMΔN variant, suggesting that PulM N-terminal peptide stabilizes PulL in the cytoplasm. We discuss the implications of these results for the T2S endopilus and type IV pilus assembly mechanisms.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10331112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond membrane components: uncovering the intriguing world of fungal sphingolipid synthesis and regulation","authors":"Sana Akhtar Usmani ,&nbsp;Mohit Kumar ,&nbsp;Khushboo Arya ,&nbsp;Basharat Ali ,&nbsp;Nitin Bhardwaj ,&nbsp;Naseem Akhtar Gaur ,&nbsp;Rajendra Prasad ,&nbsp;Ashutosh Singh","doi":"10.1016/j.resmic.2023.104087","DOIUrl":"10.1016/j.resmic.2023.104087","url":null,"abstract":"<div><p>Sphingolipids (SLs) are essential to fungal survival and represent a major class of structural and signaling lipids. Unique SL structures and their biosynthetic enzymes in filamentous fungi<span> make them an ideal drug target. Several studies have contributed towards the functional characterization of specific SL metabolism genes, which have been complemented by advanced lipidomics methods which allow accurate identification and quantification of lipid structures and pathway mapping. These studies have provided a better understanding of SL biosynthesis, degradation and regulation networks in filamentous fungi, which are discussed and elaborated here.</span></p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10633121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alleviating the virulence of Pseudomonas aeruginosa and Staphylococcus aureus by ascorbic acid nanoemulsion","authors":"Farag M. Mosallam ,&nbsp;Hisham A. Abbas ,&nbsp;Ghada H. Shaker ,&nbsp;Salwa E. Gomaa","doi":"10.1016/j.resmic.2023.104084","DOIUrl":"10.1016/j.resmic.2023.104084","url":null,"abstract":"<div><p><span><span>The high incidence of persistent multidrug resistant bacterial infections is a worldwide public health burden. Alternative strategies are required to deal with such issue including the use of drugs with anti-virulence activity. The application of nanotechnology to develop advanced Nano-materials that target quorum sensing regulated </span>virulence factors<span> is an attractive approach. Synthesis of ascorbic acid Nano-emulsion (ASC-NEs) and assessment of its activity </span></span><em>in vitro</em> against the virulence factors and its protective ability against pathogenesis as well as the effect against expression of quorum sensing genes of <span><em>Pseudomonas aeruginosa</em></span> and <span><em>Staphylococcus aureus</em></span><span><span> isolates. Ascorbic acid Nano-emulsion was characterized by DLS Zetasizer Technique, Zeta potential<span>; Transmission Electron Microscopy (TEM) and </span></span>Fourier transform infrared spectroscopy<span><span> (FT-IR). The antibacterial activity of ASC-NEs was tested by the </span>broth microdilution method and the activity of their sub-MIC against the expression of quorum sensing controlled virulence was investigated using phenotypic experiments and RT-PCR. The protective activity of ASC-NEs against </span></span><em>P. aeruginosa</em> as well as <em>S. aureus</em> pathogenesis was tested <em>in vivo</em>. Phenotypically, ASC-NEs had strong virulence inhibitory activity against the tested bacteria. The RT-PCR experiment showed that it exhibited significant QS inhibitory activity. The <em>in vivo</em> results showed that ASC-NEs protected against staphylococcal infection, however, it failed to protect mice against Pseudomonal infection. These results suggest the promising use of nanoformulations against virulence factors in multidrug resistant <em>P. aeruginosa</em> and <em>S. aureus</em>. However, further studies are required concerning the potential toxicity, clearance and phamacokinetics of the nanoformulations.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10270898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Archaea: current and potential biotechnological applications","authors":"David Aparici-Carratalá,&nbsp;Julia Esclapez,&nbsp;Vanesa Bautista,&nbsp;María-José Bonete,&nbsp;Mónica Camacho","doi":"10.1016/j.resmic.2023.104080","DOIUrl":"10.1016/j.resmic.2023.104080","url":null,"abstract":"<div><p>Archaea are microorganisms with great ability to colonize some of the most inhospitable environments in nature, managing to survive in places with extreme characteristics for most microorganisms. Its proteins and enzymes are stable and can act under extreme conditions in which other proteins and enzymes would degrade. These attributes make them ideal candidates for use in a wide range of biotechnological applications. This review describes the most important applications, both current and potential, that archaea present in Biotechnology, classifying them according to the sector to which the application is directed. It also analyzes the advantages and disadvantages of its use.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10259019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteriophages potentiate the effect of antibiotics by eradication of persister cells and killing of biofilm-forming cells","authors":"Javiera Vera-Mansilla ,&nbsp;Cecilia A. Silva-Valenzuela ,&nbsp;Patricio Sánchez ,&nbsp;Roberto C. Molina-Quiroz","doi":"10.1016/j.resmic.2023.104083","DOIUrl":"10.1016/j.resmic.2023.104083","url":null,"abstract":"<div><p>Persister cells and biofilms are associated with chronic urinary infections which are more critical when generated by multi-drug resistant bacteria. In this context, joint administration of phages and antibiotics has been proposed as an alternative approach, since it may decrease the probability to generate resistant mutants to both agents. In this work, we exposed cultures of uropathogenic <em>Escherichia coli</em> conjunctly to antibiotics and phages. We determined that MLP2 combined with antibiotics eradicates persister cells. Similarly, MLP1 and MLP3 impact viability of biofilm-forming cells when administered with ampicillin. Our findings suggest a feasible prophylactic and therapeutic use of these non-transducing phages.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10278484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The AcrAB efflux pump confers self-resistance to stilbenes in Photorhabdus laumondii","authors":"Linda Hadchity ,&nbsp;Jessica Houard ,&nbsp;Anne Lanois ,&nbsp;Amaury Payelleville ,&nbsp;Fida Nassar ,&nbsp;Maxime Gualtieri ,&nbsp;Alain Givaudan ,&nbsp;Ziad Abi Khattar","doi":"10.1016/j.resmic.2023.104081","DOIUrl":"10.1016/j.resmic.2023.104081","url":null,"abstract":"<div><p><span>The Resistance-nodulation-division (RND)-type AcrAB-TolC efflux pump contributes to multidrug resistance in Gram-negative bacteria. Recently, the bacterium </span><span><em>Photorhabdus</em><em> laumondii</em></span> TT01 has emerged as a goldmine for novel anti-infective drug discovery. Outside plants, <em>Photorhabdus</em><span> is the only Gram-negative known to produce stilbene-derivatives including 3,5-dihydroxy-4-ethyl-trans-stilbene and 3,5-dihydroxy-4-isopropyl-trans-stilbene (IPS). IPS is a bioactive polyketide which received considerable attention, mainly because of its antimicrobial properties, and is currently in late-stage clinical development as a topical treatment for psoriasis and dermatitis. To date, little is known about how </span><em>Photorhabdus</em> survives in the presence of stilbenes. We combined genetic and biochemical approaches to assess whether AcrAB efflux pump exports stilbenes in <em>P. laumondii</em>. We demonstrated that the wild-type (WT) exerts an antagonistic activity against its derivative Δ<em>acrA</em> mutant, and that is able to outcompete it in a dual-strain co-culture assay. The Δ<em>acrA</em> mutant also showed high sensitivity to 3,5-dihydroxy-4-ethyl-trans-stilbene and IPS as well as decreased IPS concentrations in its supernatant comparing to the WT. We report here a mechanism of self-resistance against stilbene derivatives of <em>P. laumondii</em> TT01, which enables these bacteria to survive under high concentrations of stilbenes by extruding them out via the AcrAB efflux pump.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10319878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}