Research in microbiology最新文献_第7页

Heat shock response in Sulfolobus acidocaldarius and first implications for cross-stress adaptation 嗜酸硫酸藻的热休克反应和交叉应激适应的初步意义。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104106

Arghya Bhowmick , Koustav Bhakta , Mousam Roy , Sayandeep Gupta , Jagriti Das , Shirsha Samanta , Somi Patranabis , Abhrajyoti Ghosh

{"title":"Heat shock response in Sulfolobus acidocaldarius and first implications for cross-stress adaptation","authors":"Arghya Bhowmick , Koustav Bhakta , Mousam Roy , Sayandeep Gupta , Jagriti Das , Shirsha Samanta , Somi Patranabis , Abhrajyoti Ghosh","doi":"10.1016/j.resmic.2023.104106","DOIUrl":"10.1016/j.resmic.2023.104106","url":null,"abstract":"<div>Sulfolobus acidocaldarius, a thermoacidophilic crenarchaeon, frequently encounters temperature fluctuations, oxidative stress, and nutrient limitations in its environment. Here, we employed a high-throughput transcriptomic analysis to examine how the gene expression of S. acidocaldarius changes when exposed to high temperatures (92 °C). The data obtained was subsequently validated using quantitative reverse transcription-PCR (qRT-PCR) analysis. Our particular focus was on genes that are involved in the heat shock response, type-II Toxin-Antitoxin systems, and putative transcription factors. To investigate how S. acidocaldarius adapts to multiple stressors, we assessed the expression of these selected genes under oxidative and nutrient stresses using qRT-PCR analysis. The results demonstrated that the gene thβ encoding the β subunit of the thermosome, as well as hsp14 and hsp20, play crucial roles in the majority of stress conditions. Furthermore, we observed overexpression of at least eight different TA pairs belonging to the type II TA systems under all stress conditions. Additionally, four common transcription factors: FadR, TFEβ, CRISPR loci binding protein, and HTH family protein were consistently overexpressed across all stress conditions, indicating their significant role in managing stress. Overall, this work provides the first insight into molecular players involved in the cross-stress adaptation of S. acidocaldarius.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10247814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Leptospiral cell wall hydrolase (LIC_10271) binding peptidoglycan, lipopolysaccharide, and laminin and the protein show LysM and M23 domains are co-existing in pathogenic species 钩端螺旋体细胞壁水解酶（LIC_10271）结合肽聚糖、脂多糖和层粘连蛋白，该蛋白显示LysM和M23结构域在致病物种中共存。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104107

Abhijit Sarma , Gunasekaran Dhandapani , Homen Phukan , Prasun Kumar Bhunia , Arun Kumar De , Debasis Bhattacharya , T. Jebasingh , Madathiparambil G. Madanan

{"title":"Leptospiral cell wall hydrolase (LIC_10271) binding peptidoglycan, lipopolysaccharide, and laminin and the protein show LysM and M23 domains are co-existing in pathogenic species","authors":"Abhijit Sarma , Gunasekaran Dhandapani , Homen Phukan , Prasun Kumar Bhunia , Arun Kumar De , Debasis Bhattacharya , T. Jebasingh , Madathiparambil G. Madanan","doi":"10.1016/j.resmic.2023.104107","DOIUrl":"10.1016/j.resmic.2023.104107","url":null,"abstract":"<div>Leptospirosis, a global reemerging zoonosis caused by the spirochete Leptospira, has severe human and veterinary implications. Cell wall hydrolase (LIC_10271) with LytM (peptidase M23) and LysM domains are found to be associated with various pathogenic bacteria. These domains regulate effects on extracellular matrix and biofilm components, which promote cell wall remodeling and pathogen dissemination in the host. In this study, we present the cloning, expression, purification, and characterization of LIC_10271. To determine the localization of LIC_10271 within the inner membrane of Leptospira, Triton X-114 subcellular fractionation and immunoblot studies were performed. Furthermore, r-LIC_10271 binds with peptidoglycan, lipopolysaccharide, and laminin in a dose-dependent manner. Analysis of the signal peptide, M23, and LysM domains revealed conservation primarily within the P1 group of Leptospira, which encompasses the most pathogenic species. Moreover, the presence of native-LIC_10271 in the inner membrane and the distribution of M23 and LysM domains across pathogenic strains indicates their potential involvement in the interaction between the host and Leptospira.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9951818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deficient Pseudomonas aeruginosa in MlaA/VacJ outer membrane lipoprotein shows decrease in rhamnolipids secretion, motility, and biofilm formation, and increase in fluoroquinolones susceptibility and innate immune response MlaA/VacJ外膜脂蛋白中的铜绿假单胞菌缺陷显示鼠李糖脂分泌、运动性和生物膜形成减少，氟喹诺酮类药物敏感性和先天免疫反应增加。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104132

M. Kaur , J.M. Buyck , F. Goormaghtigh , J.-L. Decout , N. Mozaheb , M.-P. Mingeot-Leclercq

{"title":"Deficient Pseudomonas aeruginosa in MlaA/VacJ outer membrane lipoprotein shows decrease in rhamnolipids secretion, motility, and biofilm formation, and increase in fluoroquinolones susceptibility and innate immune response","authors":"M. Kaur , J.M. Buyck , F. Goormaghtigh , J.-L. Decout , N. Mozaheb , M.-P. Mingeot-Leclercq","doi":"10.1016/j.resmic.2023.104132","DOIUrl":"10.1016/j.resmic.2023.104132","url":null,"abstract":"<div>Pseudomonas aeruginosa, a Gram-negative bacterium that causes severe hospital acquired infections poses threat by its ability for adaptation to various growth modes and environmental conditions and by its intrinsic resistance to antibiotics. The latter is mainly due to the outer membrane (OM) asymmetry which is maintained by the Mla pathway resulting in the retrograde transport of glycerophospholipids from the OM to the inner membrane. It comprises six Mla proteins, including MlaA, an OM lipoprotein involved in the removal of glycerophospholipids mislocalized at the outer leaflet of OM.To investigate the role of P. aeruginosa OM asymmetry especially MlaA, this study investigated the effect of mlaA deletion on (i) the susceptibility to antibiotics, (ii) the secretion of virulence factors, the motility, biofilm formation, and (iii) the inflammatory response.mlaA deletion in P. aeruginosa ATCC27853 results in phenotypic changes including, an increase in fluoroquinolones susceptibility and in PQS (Pseudomonas Quinolone Signal) and TNF-α release and a decrease in rhamnolipids secretion, motility and biofilm formation.Investigating how the mlaA knockout impacts on antibiotic susceptibility, bacterial virulence and innate immune response will help to elucidate the biological significance of the Mla system and contribute to the understanding of MlaA in P. aeruginosa OM asymmetry.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0923250823001079/pdfft?md5=0ddbed0060c793d80f891fde818c0b30&pid=1-s2.0-S0923250823001079-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10146831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dethiobiotin uptake and utilization by bacteria possessing bioYB operon 具有生物YB操纵子的细菌对脱硫生物素的吸收和利用。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104131

Tomoki Ikeda , Tetsuhiro Ogawa , Toshihiro Aono

{"title":"Dethiobiotin uptake and utilization by bacteria possessing bioYB operon","authors":"Tomoki Ikeda , Tetsuhiro Ogawa , Toshihiro Aono","doi":"10.1016/j.resmic.2023.104131","DOIUrl":"10.1016/j.resmic.2023.104131","url":null,"abstract":"<div>Biotin is an essential vitamin for all organisms. Some bacteria cannot synthesize biotin and live by acquiring biotin from the environment. Bacterial biotin transporters (BioY) are classified into three mechanistic types. The first forms the BioMNY complex with ATPase (BioM) and transmembrane protein (BioN). The second relies on a promiscuous energy coupling module. The third functions independently. One-third of bioY genes spread in bacteria cluster with bioM and bioN on the genomes, and the rest does not. Interestingly, some bacteria have the bioY gene clustering with bioB gene, which encodes biotin synthase, an enzyme that converts dethiobiotin to biotin, on their genome. This bioY-bioB cluster is observed even though these bacteria cannot synthesize biotin. Azorhizobium caulinodans ORS571, a rhizobium of tropical legume Sesbania rostrata, is one of such bacteria. In this study using this bacterium, we demonstrated that the BioY linked to BioB could transport not only biotin but also dethiobiotin, and the combination of BioY and BioB contributed to the growth of A. caulinodans ORS571 in a biotin-deficient but dethiobiotin-sufficient environment. We propose that such environment universally exists in the natural world, and the identification of such environment will be a new subject in the field of microbial ecology.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10484351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding the chromosome-scale genome of the nutrient-rich Agaricus subrufescens: a resource for fungal biology and biotechnology 解码营养丰富的亚陆生蘑菇的染色体规模基因组：真菌生物学和生物技术的资源。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104116

Carlos Godinho de Abreu , Luiz Fernando Wurdig Roesch , Fernando Dini Andreote , Saura Rodrigues Silva , Tatiana Silveira Junqueira de Moraes , Diego Cunha Zied , Félix Gonçalves de Siqueira , Eustáquio Souza Dias , Alessandro M. Varani , Victor Satler Pylro

{"title":"Decoding the chromosome-scale genome of the nutrient-rich Agaricus subrufescens: a resource for fungal biology and biotechnology","authors":"Carlos Godinho de Abreu , Luiz Fernando Wurdig Roesch , Fernando Dini Andreote , Saura Rodrigues Silva , Tatiana Silveira Junqueira de Moraes , Diego Cunha Zied , Félix Gonçalves de Siqueira , Eustáquio Souza Dias , Alessandro M. Varani , Victor Satler Pylro","doi":"10.1016/j.resmic.2023.104116","DOIUrl":"10.1016/j.resmic.2023.104116","url":null,"abstract":"<div>Agaricus subrufescens, also known as the “sun mushroom,” has significant nutritional and medicinal value. However, its short shelf life due to the browning process results in post-harvest losses unless it's quickly dehydrated. This restricts its availability to consumers in the form of capsules. A genome sequence of A. subrufescens may lead to new cultivation alternatives or the application of gene editing strategies to delay the browning process. We assembled a chromosome-scale genome using a hybrid approach combining Illumina and Nanopore sequencing. The genome was assembled into 13 chromosomes and 31 unplaced scaffolds, totaling 44.5 Mb with 96.5% completeness and 47.24% GC content. 14,332 protein-coding genes were identified, with 64.6% of the genome covered by genes and 23.41% transposable elements. The mitogenome was circularized and encoded fourteen typical mitochondrial genes. Four polyphenol oxidase (PPO) genes and the Mating-type locus were identified. Phylogenomic analysis supports the placement of A. subrufescens in the Agaricomycetes clade. This is the first available genome sequence of a strain of the “sun mushroom.” Results are available through a Genome Browser (https://plantgenomics.ncc.unesp.br/gen.php?id=Asub) and can support further fungal biological and genomic studies.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10044329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modification of the second PEP4-allele facilitates an industrial Saccharomyces cerevisiae to tolerate tartaric acid stress 第二个PEP4等位基因的修饰促进工业酿酒酵母耐受酒石酸应激。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104109

Hongbo Zhang, Xiaomei Yang, Chi Shen, Jianqiu Sun, Yuhang Lu, Wanting Hu, Hongfei Yao, Wenhao Zhao

{"title":"Modification of the second PEP4-allele facilitates an industrial Saccharomyces cerevisiae to tolerate tartaric acid stress","authors":"Hongbo Zhang, Xiaomei Yang, Chi Shen, Jianqiu Sun, Yuhang Lu, Wanting Hu, Hongfei Yao, Wenhao Zhao","doi":"10.1016/j.resmic.2023.104109","DOIUrl":"10.1016/j.resmic.2023.104109","url":null,"abstract":"<div>The practical significance of constructing robust industrial production strains against organic acid stress lies not only in improving fermentation efficiency but also in reducing manufacturing costs. In a previous study, we constructed an industrial Saccharomyces cerevisiae strain by modifying another PEP4-allele of a mutant that already had one PEP4-allele disrupted. This modification enhanced cellular tolerance to citric acid stress during growth. Unlike citric acid, which S. cerevisiae can consume, tartaric acid is often added to grape must during winemaking to increase total acidity and is not metabolizable. The results of the present study indicate that the modification of the second PEP4-allele improves the cellular tolerance of the strain with one PEP4-allele disrupted against tartaric acid stress during growth and contributes to maintaining intracellular pH homeostasis in cells subjected to tartaric acid stress. Moreover, under tartaric acid stress, a significant improvement in glucose-ethanol conversion performance, conferred by the modification of the second PEP4-allele, was observed. This study not only broadens our understanding of the role of the PEP4-allele in cellular regulation but also provides a prospective approach to reducing the concentration of sulfur dioxide used in winemaking.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9951819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IS257-mediated amplification of tet(L) variant as a novel mechanism of enhanced tigecycline resistance in Staphylococcus cohnii IS257介导的tet（L）变异体扩增是提高科氏葡萄球菌对替加环素耐药性的新机制。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-11-01 DOI: 10.1016/j.resmic.2023.104114

Hong Yao , Hongjie Xing , Nannan Wang , Likuan Zhang , Stefan Schwarz , Chenglong Li , Chang Cai , Chunyan Xu , Xiang-Dang Du

{"title":"IS257-mediated amplification of tet(L) variant as a novel mechanism of enhanced tigecycline resistance in Staphylococcus cohnii","authors":"Hong Yao , Hongjie Xing , Nannan Wang , Likuan Zhang , Stefan Schwarz , Chenglong Li , Chang Cai , Chunyan Xu , Xiang-Dang Du","doi":"10.1016/j.resmic.2023.104114","DOIUrl":"10.1016/j.resmic.2023.104114","url":null,"abstract":"<div>The mechanism of enhanced tigecycline MIC in Staphylococcus cohnii after in vitro tigecycline exposure was investigated. S. cohnii 11-B-312 was exposed to incremental concentrations of tigecycline (2–32 mg/L) and the mutants growing at 8, 16 and 32 mg/L were determined by AST and WGS. Copy number and relative transcription level of the tet(L) gene were determined by quantitative PCR. The fitness cost was evaluated by growth kinetics and competition assays. The results revealed that enhanced tigecycline MIC was identified in S. cohnii mutants. Copy number and relative transcription level of tet(L) in the mutants increased 8-, 20-, and 23-fold and 20-, 34-, and 39-fold in the presence of 8, 16, and 32 mg/L tigecycline, respectively. The read-mapping depth ratio analysis indicated that a multidrug resistance region carrying the tet(L) variant has a gradually increased copy number, correlating with the tigecycline selection pressure. S. cohnii strain 11-B-312_32 had a fitness cost, and enhanced tigecycline MIC can revert to the initial level in the absence of tigecycline. In summary, enhanced tigecycline MIC develops with extensive amplification of an IS257-flanked tet(L)-carrying segment in S. cohnii. IS257 seems to play a vital role in the gain and loss of the amplification product.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9984927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corynebacterium ramonii sp. nov., a novel toxigenic member of the Corynebacterium diphtheriae species complex 雷莫尼棒状杆菌（Corynebacterium ramonii sp.nov.），白喉棒状杆菌物种复合体的一个新的产毒成员。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-09-01 DOI: 10.1016/j.resmic.2023.104113

Chiara Crestani , Gabriele Arcari , Annie Landier , Virginie Passet , Dorian Garnier , Sylvie Brémont , Nathalie Armatys , Annick Carmi-Leroy , Julie Toubiana , Edgar Badell , Sylvain Brisse

{"title":"Corynebacterium ramonii sp. nov., a novel toxigenic member of the Corynebacterium diphtheriae species complex","authors":"Chiara Crestani , Gabriele Arcari , Annie Landier , Virginie Passet , Dorian Garnier , Sylvie Brémont , Nathalie Armatys , Annick Carmi-Leroy , Julie Toubiana , Edgar Badell , Sylvain Brisse","doi":"10.1016/j.resmic.2023.104113","DOIUrl":"10.1016/j.resmic.2023.104113","url":null,"abstract":"<div>The Corynebacterium diphtheriae species complex comprises seven bacterial species, including Corynebacterium ulcerans, a zoonotic pathogen from multiple animal species. In this work, we characterise phenotypically and genotypically isolates belonging to two C. ulcerans lineages. Results from phylogenetic analyses, in silico DNA–DNA hybridization (DDH) and MALDI-TOF spectra differentiate lineage 2 from C. ulcerans lineage 1, which, together with their distinct transmission dynamics (probable human-to-human vs animal-to-human), indicates that lineage 2 is a separate Corynebacterium species, which we propose to name Corynebacterium ramonii. This species is of particular medical interest considering that its human-to-human transmission is likely, and that some C. ramonii isolates carry the diphtheria toxin gene.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10320457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Genome-based analyses of family Oxalobacteraceae reveal the taxonomic classification 基于基因组的分析揭示了牛杆菌科的分类。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-09-01 DOI: 10.1016/j.resmic.2023.104076

Tengfei Ma, Han Xue, Chungen Piao, Ning Jiang, Yong Li

{"title":"Genome-based analyses of family Oxalobacteraceae reveal the taxonomic classification","authors":"Tengfei Ma, Han Xue, Chungen Piao, Ning Jiang, Yong Li","doi":"10.1016/j.resmic.2023.104076","DOIUrl":"10.1016/j.resmic.2023.104076","url":null,"abstract":"<div>Family Oxalobacteraceae is known for the indicator of bacterial diversity in the environment and many of which are important beneficial bacteria. Previous studies on the taxonomic structure of family Oxalobacteraceae mostly relied on 16S rRNA gene analysis, or core-genome phylogeny of a limited number of species and resulted in taxonomic confusion within several genera. Developments in sequencing technologies have allowed more genome sequences to be obtained, enabling the revision of family Oxalobacteraceae. Here, we report a comprehensive analysis of phylogenomic trees, concatenated protein and up-to-date bacterial core gene phylogenetic trees, and genomic metrics for genus demarcation on 135 genomes of Oxalobacteraceae species to elucidate their interrelationships. Following this framework for classification of species in family Oxalobacteraceae, all the proposed genera formed monophyletic lineages in the phylogenomic trees and could also be clearly separated from others in the genomic similarity indexes of average amino acid identity, percentage of conserved proteins and core-proteome average amino acid identity.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10616052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacillus pfraonensis sp. nov., a new strain isolated from a probiotic feed additive with low cytotoxicity and antimicrobial activity 芽孢杆菌pfraonensis sp.nov.，一株从益生菌饲料添加剂中分离得到的具有低细胞毒性和抗菌活性的新菌株。

IF 2.6 4区生物学

Research in microbiology Pub Date : 2023-09-01 DOI: 10.1016/j.resmic.2023.104090

Fengru Deng , Yunsheng Chen , Huiying Xiao , Chuying Yao , Jiaqi Chen , Yuanting Zhao , Yapei Jiang , Jiahang Chen , Yiqun Deng , Jikai Wen

{"title":"Bacillus pfraonensis sp. nov., a new strain isolated from a probiotic feed additive with low cytotoxicity and antimicrobial activity","authors":"Fengru Deng , Yunsheng Chen , Huiying Xiao , Chuying Yao , Jiaqi Chen , Yuanting Zhao , Yapei Jiang , Jiahang Chen , Yiqun Deng , Jikai Wen","doi":"10.1016/j.resmic.2023.104090","DOIUrl":"10.1016/j.resmic.2023.104090","url":null,"abstract":"<div>Probiotic products containing living microorganisms are gaining popularity, increasing the importance of their taxonomic status. A Bacillus-like isolate, 70 b, cultured from a probiotic feed additive, was ambiguity in taxonomic assignment and could be a novel member of Bacillus cereus group. The results of colony and cellular morphology, physiological and biochemical analysis mainly including growth performance, carbon source utilization, and rMLST and MLST were not conclusive. Fatty acids profile and molecular genetic analysis especially ANI, DDH, and core genome SNPs-based phylogenetic tree confirmed 70 b as one novel species of B. cereus group and proposed as Bacillus pfraonensis sp. nov. Comparative genomic analysis revealed the genetic differences between 70 b and other species of B. cereus group. Pseudomycoicidin was identified in 70 b. 70 b was active against multidrug-resistant pathogenic strains MRSA. The findings support 70 b is a novel species with low cytotoxicity and antimicrobial activity, and provides a better understanding of its unique characteristics and probiotic potential, and exploration of bioactive potential.</div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10270906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0