Research in microbiology最新文献

{"title":"Rates of iron(III) reduction coupled to elemental sulfur or tetrathionate oxidation by acidophilic microorganisms and detection of sulfur intermediates","authors":"Anja Breuker,&nbsp;Axel Schippers","doi":"10.1016/j.resmic.2023.104110","DOIUrl":"10.1016/j.resmic.2023.104110","url":null,"abstract":"<div><p>Bioleaching processes and acid mine drainage (AMD) generation are mainly driven by aerobic microbial iron(II) and inorganic sulfur/compound oxidation. Dissimilatory iron(III) reduction coupled to sulfur/compound oxidation (DIRSO) by acidophilic microorganisms has been described for anaerobic cultures, but iron reduction was observed under aerobic conditions as well. Aim of this study was to explore reaction rates and mechanisms of this process. Cell-specific iron(III) reduction rates for different <em>Acidithiobacillus</em> (<em>At.</em>) strains during batch culture growth or stationary phase with iron(III) (∼40 mM) as electron acceptor and elemental sulfur or tetrathionate as electron donor (1% or 5 mM, respectively) were determined. The rates were highest under anaerobic conditions for the <em>At. ferrooxidans</em> type strain with 6.8 × 10⁶ and 1.1 × 10⁷ reduced iron(III) ions per second per cell for growth on elemental sulfur and tetrathionate, respectively. The iron(III) reduction rates were somehow lower for the anaerobically sulfur grown archaeon <em>Ferroplasma acidiphilum</em>, and lowest for the sulfur grown <em>At. caldus</em> type strain under aerobic conditions (1.7 × 10⁶ and 7.3 × 10⁴ reduced iron(III) ions per second per cell, respectively). The rates for five strains of <em>At. thiooxidans</em> (aerobe) were in between those for <em>At. ferrooxidans</em> (anaerobe) and <em>At. caldus</em> (aerobe). There was no pronounced pH dependence of iron(III) reduction rates in the range of pH 1.0–1.9 for the type strains of all species but rates increased with increasing pH for four other <em>At. thiooxidans</em> strains. Thiosulfate as sulfur intermediate was found for <em>At. ferrooxidans</em> during anaerobic growths on tetrathionate and iron(III) but not during anaerobic growths on elemental sulfur and iron(III), and a small concentration was measured during aerobic growths on tetrathionate without iron(III). For the <em>At. thiooxidans</em> type strain thiosulfate was found with tetrathionate grown cells under aerobic conditions in presence and absence of iron(III), but not with sulfur grown cells. Evidence for hydrogen sulfide production at low pH was found for the <em>At. ferrooxidans</em> as well as the <em>At. thiooxidans</em> type strains during microaerophilic growth on elemental sulfur and for <em>At. ferrooxidans</em> during anaerobic growths on tetrathionate and iron(III). The occurrence of sulfur compound intermediates supports the hypothesis that chemical reduction of iron(III) ions takes place by sulfur compounds released by the microbial cells.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 1","pages":"Article 104110"},"PeriodicalIF":2.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0923250823000852/pdfft?md5=113ed6974c2a60c49a0f98645f745eb4&pid=1-s2.0-S0923250823000852-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9943400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of element yield, bacterial community structure and the impact of carbon sources for bioleaching rare earth elements from high grade monazite","authors":"Melissa K. Corbett ,&nbsp;April Gifford ,&nbsp;Nick Fimognari ,&nbsp;Elizabeth L.J. Watkin","doi":"10.1016/j.resmic.2023.104133","DOIUrl":"10.1016/j.resmic.2023.104133","url":null,"abstract":"<div><p>Rare earth element (REE) recovery from waste streams, mine tailings or recyclable components using bioleaching is gaining traction due to the shortage and security of REE supply as well as the environmental problems that occur from processing and refining. Four heterotrophic microbial species with known phosphate solubilizing capabilities were evaluated for their ability to leach REE from a high-grade monazite when provided with either galactose, fructose or maltose. Supplying fructose resulted in the greatest amount of REE leached from the ore due to the largest amount of organic acid produced. Gluconic acid was the dominant organic acid identified produced by the cultures, followed by acetic acid. The monazite proved difficult to leach with the different carbon sources, with preferential release of Ce over La, Nd and Pr.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 1","pages":"Article 104133"},"PeriodicalIF":2.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0923250823001080/pdfft?md5=c629c7b62e1c20b070140e1adb7d68e8&pid=1-s2.0-S0923250823001080-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10178287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shedding light on the electron transfer chain of a moderately acidophilic iron oxidizer: characterization of recombinant HiPIP-41, CytC-18 and CytC-78 derived from Ferrovum sp. PN-J47-F6","authors":"Sophie R. Ullrich,&nbsp;Helena Fuchs,&nbsp;Michael Schlömann","doi":"10.1016/j.resmic.2023.104088","DOIUrl":"10.1016/j.resmic.2023.104088","url":null,"abstract":"<div><p>Efficient electron transfer from the donor to the acceptor couple presents a necessary requirement for acidophilic and neutrophilic iron oxidizers due to the low energy yield of aerobic ferrous iron oxidation. Involved periplasmic electron carriers are very diverse in these bacteria and show adaptations to the respective thermodynamic constraints such as a more positive redox potential reported for extreme acidophilic <em>Acidithiobacillus</em> spp. Respiratory chain candidates of moderately acidophilic members of the genus <em>Ferrovum</em> share similarities with both their neutrophilic iron oxidizing relatives and the more distantly related <em>Acidithiobacillus</em> spp. We examined our previous omics-based conclusions on the potential electron transfer chain in <em>Ferrovum</em> spp. by characterizing the three redox protein candidates CytC-18, CytC-78 and HiPIP-41 of strain PN-J47-F6 which were produced as recombinant proteins in <em>Eschericha coli.</em> UV/Vis-based redox assays suggested that HiPIP-41 has a very positive redox potential while redox potentials of CytC-18 and CytC-78 are more negative than their counterparts in <em>Acidithiobacillus</em> spp. Far Western dot blotting demonstrated interactions between all three recombinant redox proteins while redox assays showed the electron transfer from HiPIP-41 to either of the cytochromes. Altogether, CytC-18, CytC-78 and HiPIP-41 indeed represent very likely candidates of the electron transfer in <em>Ferrovum</em> sp. PN-J4-F6.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 1","pages":"Article 104088"},"PeriodicalIF":2.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0923250823000633/pdfft?md5=30ef0e7e14161084490db12e5d7eb88e&pid=1-s2.0-S0923250823000633-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9674595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simulating compatible solute biosynthesis using a metabolic flux model of the biomining acidophile, Acidithiobacillus ferrooxidans ATCC 23270","authors":"Himel Nahreen Khaleque ,&nbsp;Hadi Nazem-Bokaee ,&nbsp;Yosephine Gumulya ,&nbsp;Ross P. Carlson ,&nbsp;Anna H. Kaksonen","doi":"10.1016/j.resmic.2023.104115","DOIUrl":"10.1016/j.resmic.2023.104115","url":null,"abstract":"<div><p>Halotolerant, acidophilic, bioleaching microorganisms are crucial to biomining operations that utilize saline water. Compatible solutes play an important role in the adaptation of these microorganisms to saline environments. <em>Acidithiobacillus ferrooxidans</em> ATCC 23270, an iron- and sulfur-oxidizing acidophilic bacterium, synthesizes trehalose as its native compatible solute but is still sensitive to salinity. Recently, halotolerant bioleaching bacteria were found to use ectoine as their key compatible solute. Previously, bioleaching bacteria were recalcitrant to genetic manipulation; however, recent advancements in genetic tools and techniques allow successful genetic modification of <em>A. ferrooxidans</em> ATCC 23270. Therefore, this study aimed to test, <em>in silico</em>, the effect of native and synthetic compatible solute biosynthesis by <em>A. ferrooxidans</em> ATCC 23270 on its growth and metabolism. Metabolic network flux modelling was used to provide a computational framework for the prediction of metabolic fluxes during production of native and synthetic compatible solutes by <em>A. ferrooxidans</em> ATCC 23270, <em>in silico</em>. Complete pathways for trehalose biosynthesis by the bacterium are proposed and captured in the updated metabolic model including a newly discovered UDP-dependent trehalose synthesis pathway. Finally, the effect of nitrogen sources on compatible solute production was simulated and showed that using nitrogen gas as the sole nitrogen source enables the ectoine-producing ‘engineered’ microbe to oxidize up to 20% more ferrous iron in comparison to the native microbe that only produces trehalose. Therefore, the predictive outcomes of the model have the potential to guide the design and optimization of a halotolerant strain of <em>A. ferrooxidans</em> ATCC 23270 for saline bioleaching operations.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 1","pages":"Article 104115"},"PeriodicalIF":2.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0923250823000906/pdfft?md5=e2ca8dfe1f5c3c7a492bbdffe6ec30ce&pid=1-s2.0-S0923250823000906-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9984930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Special issue on International Biohydrometallurgy Symposium (IBS) 2022","authors":"Elizabeth Watkin,&nbsp;Axel Schippers,&nbsp;Melissa Corbett","doi":"10.1016/j.resmic.2023.104174","DOIUrl":"10.1016/j.resmic.2023.104174","url":null,"abstract":"","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 1","pages":"Article 104174"},"PeriodicalIF":2.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0923250823001511/pdfft?md5=d54181ff290c80ab3c1ac1c0ba1c002c&pid=1-s2.0-S0923250823001511-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138885986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial immobilisation and adaptation to Cu2+ enhances microbial Fe2+ oxidation for bioleaching of printed circuit boards in the presence of mixed metal ions","authors":"Musa D. Maluleke,&nbsp;Athanasios Kotsiopoulos,&nbsp;Elaine Govender-Opitz,&nbsp;Susan T.L. Harrison","doi":"10.1016/j.resmic.2023.104148","DOIUrl":"10.1016/j.resmic.2023.104148","url":null,"abstract":"<div><p>A circular economy requires effective re-use of finite resources, such as metals from waste electrical and electronic equipment (WEEE). Bioleaching for extraction and recovery of base metals from printed circuit boards (PCBs) before recovering precious metals has potential to increase metal circularity. However, inhibition by base metals released from the PCBs and accumulated in PCB leachates on microbial Fe²⁺ oxidation, a critical bioleaching sub-process for Fe³⁺ regeneration, can limit this approach. Here, we explore the potential of microbial immobilisation on polyurethane foam (PUF) and adaptation to cupric ions to minimise inhibition by mixed metals released from PCBs, particularly zinc, nickel, and tin, and enhancing Fe²⁺ oxidation rates in PCB bioleaching systems. A mixed mesophilic culture dominant in <em>Leptospirillum ferriphilum, Acidiplasma cupricumulans</em> and <em>Acidithiobacillus caldus</em> was immobilised on PUF and adapted to 6 g/L Cu²⁺. Tolerance of Cu-adapted immobilised cells to the inhibitory metal ions Zn²⁺, Ni²⁺, and Sn²⁺, as individual (0–10 g/L) and mixed metal ions at concentrations typically leached from PCBs at solids loadings of 0–20% (mass/volume) was compared to that of non-adapted immobilised cells. Further, the impact of solutes from PCB leachates was evaluated. Inhibition by individual metal ions decreased in the order Sn²⁺ &gt; Ni²⁺ &gt; Zn²⁺. Inhibition of ferrous iron oxidation by mixed metal ions was synergistic with respect to individual metal ions. PCB leachates were more inhibitory than both mixed and individual metal ions even where metal concentration was low. Cu-adapted immobilised cells exhibited higher tolerance to increasing concentrations of inhibitory metal ions than non-adapted cells. These results are promising for the application of Cu-adapted cells in the bioleaching of PCBs and multi-metal concentrates.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 1","pages":"Article 104148"},"PeriodicalIF":2.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0923250823001237/pdfft?md5=90b029c273a50b5d2921ff92c90ab130&pid=1-s2.0-S0923250823001237-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41183487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alicyclobacillus sp. SO9, a novel halophilic acidophilic iron-oxidizing bacterium isolated from a tailings-contaminated beach, and its effect on copper extraction from chalcopyrite in the presence of high chloride concentration","authors":"Dieu Huynh ,&nbsp;Götz Haferburg ,&nbsp;Boyke Bunk ,&nbsp;Stefan R. Kaschabek ,&nbsp;Wolfgang Sand ,&nbsp;Michael Schlömann","doi":"10.1016/j.resmic.2023.104150","DOIUrl":"10.1016/j.resmic.2023.104150","url":null,"abstract":"<div><p>Many acidophilic iron-oxidizing bacteria used in the mining industry for the bioleaching of sulfidic minerals are intolerant to high chloride concentrations, resulting in problems where chloride occurs in the deposit at high concentrations or only seawater is available. In search for strains tolerating such conditions a tetrathionate- and iron-oxidizing bacterium was isolated from a tailings-contaminated beach sample at Portman Bay, Cartagena-La Union mining district, Spain, in the presence of 20 g l⁻¹ (0.34 M) sodium chloride. The isolate was able to form spores, did not grow in the absence of NaCl, and oxidized ferrous iron in the presence of up to 1.5 M (∼87 g l⁻¹) NaCl. Genome sequencing based on a combination of Illumina and PacBio reads revealed two contigs, a circular bacterial chromosome of 5.2 Mbp and a plasmid of 90 kbp, respectively. The chromosome comprised seven different 16S rRNA genes. Submission of the chromosome to the Type (Strain) Genome Server (TYGS) without preselection of similar sequences revealed exclusively type strains of the genus <em>Alicyclobacillus</em>. In the TYGS analyses the respective most similar species were dependent on whether the final tree was derived from just 16S rRNA, from the genomes, or from the proteomes. Thus, TYGS analysis clearly showed that isolate SO9 represents a novel species of the genus <em>Alicyclobacillus</em>. In the presence of artificial seawater with almost 0.6 M chloride, the addition of <em>Alicyclobacillus</em> sp. SO9 improved copper dissolution from chalcopyrite (CuFeS₂) compared to abiotic leaching without bacteria. The new isolate SO9, therefore, has potential for bioleaching at elevated chloride concentrations.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"175 1","pages":"Article 104150"},"PeriodicalIF":2.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0923250823001250/pdfft?md5=3d3de41ff290b5f4c15f01072742544e&pid=1-s2.0-S0923250823001250-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71485496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence, antimicrobial resistance, and genetic lineages of nasal Staphylococcus aureus among medical students at a Spanish University: Detection of the MSSA-CC398-IEC-type-C subclade.","authors":"Nerea C. Rosales-González, Margarita González-Martín, Idris Nasir Abdullahi, Ma Teresa Tejedor-Junco, Javier Latorre-Fernández, Carmen Torres","doi":"10.1016/j.resmic.2023.104176","DOIUrl":"https://doi.org/10.1016/j.resmic.2023.104176","url":null,"abstract":"","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"76 ","pages":"104176"},"PeriodicalIF":2.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139026010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Title page","authors":"","doi":"10.1016/S0923-2508(23)00136-5","DOIUrl":"https://doi.org/10.1016/S0923-2508(23)00136-5","url":null,"abstract":"","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 8","pages":"Article 104161"},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0923250823001365/pdfft?md5=d0b512ddcb0ab467f08ce93d33603a19&pid=1-s2.0-S0923250823001365-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138465746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphate starvation is accompanied by disturbance of intracellular cysteine homeostasis in Escherichia coli","authors":"Galina V. Smirnova,&nbsp;Aleksey V. Tyulenev,&nbsp;Kseniya V. Bezmaternykh,&nbsp;Nadezda G. Muzyka,&nbsp;Vadim Y. Ushakov,&nbsp;Oleg N. Oktyabrsky","doi":"10.1016/j.resmic.2023.104108","DOIUrl":"10.1016/j.resmic.2023.104108","url":null,"abstract":"<div><p>Metabolic rearrangements that occur during depletion of essential nutrients can lead to accumulation of potentially dangerous metabolites. Here we showed that depletion of phosphate (P_i<span>), accompanied by a sharp inhibition of growth and respiration, caused a transient excess of intracellular cysteine due to a decrease in the rate of protein synthesis. High cysteine level can be dangerous due to its ability to produce ROS and reduce Fe</span>³⁺ to Fenton-reactive Fe²⁺. To prevent these negative effects, excess cysteine was mainly incorporated into glutathione (GSH), the intracellular level of which increased by 3 times, and was also exported to the medium and partially degraded to form H₂S with participation of 3-mercaptopyruvate sulfotransferase (3MST). The addition of P_i to starving cells led to a sharp recovery of respiration and growth, GSH efflux into the medium and K⁺ influx into the cells. A pronounced coupling of P_i, GSH, and K⁺ fluxes was shown upon P_i<span> depletion and addition, which may be necessary to maintain the ionic balance in the cytoplasm. We suggest that processes aimed at restoring cysteine homeostasis may be an integral part of the universal response to stress under different types of stress and for different types of bacteria.</span></p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"174 8","pages":"Article 104108"},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10247813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}